Label-free multi-photon imaging using a compact femtosecond fiber laser mode-locked by carbon nanotube saturable absorber
Identifieur interne : 000191 ( Pmc/Checkpoint ); précédent : 000190; suivant : 000192Label-free multi-photon imaging using a compact femtosecond fiber laser mode-locked by carbon nanotube saturable absorber
Auteurs : K. Kieu ; S. Mehravar ; R. Gowda ; R. A. Norwood ; N. PeyghambarianSource :
- Biomedical Optics Express [ 2156-7085 ] ; 2013.
Abstract
We demonstrate label-free multi-photon imaging of biological samples using a compact Er3+-doped femtosecond fiber laser mode-locked by a single-walled carbon nanotube (CNT). These compact and low cost lasers have been developed by various groups but they have not been exploited for multiphoton microscopy. Here, it is shown that various multiphoton imaging modalities (e.g. second harmonic generation (SHG), third harmonic generation (THG), two-photon excitation fluorescence (TPEF), and three-photon excitation fluorescence (3PEF)) can be effectively performed on various biological samples using a compact handheld CNT mode-locked femtosecond fiber laser operating in the telecommunication window near 1560nm. We also show for the first time that chlorophyll fluorescence in plant leaves and diatoms can be observed using 1560nm laser excitation via three-photon absorption.
Url:
DOI: 10.1364/BOE.4.002187
PubMed: 24156074
PubMed Central: 3799676
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<front><journal-meta><journal-id journal-id-type="nlm-ta">Biomed Opt Express</journal-id><journal-id journal-id-type="iso-abbrev">Biomed Opt Express</journal-id><journal-id journal-id-type="publisher-id">BOE</journal-id><journal-title-group><journal-title>Biomedical Optics Express</journal-title></journal-title-group><issn pub-type="epub">2156-7085</issn><publisher><publisher-name>Optical Society of America</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">24156074</article-id><article-id pub-id-type="pmc">3799676</article-id><article-id pub-id-type="publisher-id">193937</article-id><article-id pub-id-type="doi">10.1364/BOE.4.002187</article-id><article-categories><subj-group subj-group-type="heading"><subject>Microscopy</subject></subj-group><subj-group subj-group-type="article-type"><subject>Research-Article</subject></subj-group></article-categories><title-group><article-title>Label-free multi-photon imaging using a compact femtosecond fiber laser mode-locked by carbon nanotube saturable absorber</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Kieu</surname><given-names>K.</given-names></name><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib><contrib contrib-type="author"><name><surname>Mehravar</surname><given-names>S.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Gowda</surname><given-names>R.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Norwood</surname><given-names>R. A.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Peyghambarian</surname><given-names>N.</given-names></name></contrib><aff id="aff1">College of Optical Sciences, University of Arizona, Tucson, AZ 85721,<country>USA</country></aff></contrib-group><author-notes><corresp id="cor1"><label>*</label><email xlink:href="kkieu@optics.arizona.edu">kkieu@optics.arizona.edu</email></corresp></author-notes><pub-date pub-type="epub"><day>17</day><month>9</month><year>2013</year></pub-date><pub-date pub-type="collection"><day>01</day><month>10</month><year>2013</year></pub-date><pub-date pub-type="pmc-release"><day>17</day><month>9</month><year>2013</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on the
<volume>4</volume><issue>10</issue><fpage>2187</fpage><lpage>2195</lpage><history><date date-type="received"><day>15</day><month>7</month><year>2013</year></date><date date-type="rev-recd"><day>06</day><month>8</month><year>2013</year></date><date date-type="accepted"><day>21</day><month>8</month><year>2013</year></date></history><permissions><copyright-statement>© 2013 Optical Society of America</copyright-statement><copyright-year>2013</copyright-year><copyright-holder>OSA</copyright-holder></permissions><abstract><p>We demonstrate label-free multi-photon imaging of biological samples using a compact Er<sup>3+</sup>-doped femtosecond fiber laser mode-locked by a single-walled carbon nanotube (CNT). These compact and low cost lasers have been developed by various groups but they have not been exploited for multiphoton microscopy. Here, it is shown that various multiphoton imaging modalities (e.g. second harmonic generation (SHG), third harmonic generation (THG), two-photon excitation fluorescence (TPEF), and three-photon excitation fluorescence (3PEF)) can be effectively performed on various biological samples using a compact handheld CNT mode-locked femtosecond fiber laser operating in the telecommunication window near 1560nm. We also show for the first time that chlorophyll fluorescence in plant leaves and diatoms can be observed using 1560nm laser excitation via three-photon absorption.</p></abstract><kwd-group kwd-group-type="OCIS"><title>OCIS codes: </title><kwd>(170.5810) Scanning microscopy</kwd><kwd>(180.6900) Three-dimensional microscopy</kwd><kwd>(140.7090) Ultrafast lasers</kwd><kwd>(190.4180) Multiphoton processes</kwd></kwd-group><funding-group><award-group id="sp1"><funding-source>Air Force Office of Scientific Research (AFOSR)<named-content content-type="doi">10.13039/100000181</named-content></funding-source><award-id>FA9550-10-1-0555</award-id></award-group><award-group id="sp2"><funding-source>Directorate for Engineering (ENG)<named-content content-type="doi">10.13039/100000084</named-content></funding-source><award-id>EEC-0812072</award-id></award-group></funding-group></article-meta></front></pmc><affiliations><list></list><tree><noCountry><name sortKey="Gowda, R" sort="Gowda, R" uniqKey="Gowda R" first="R." last="Gowda">R. Gowda</name><name sortKey="Kieu, K" sort="Kieu, K" uniqKey="Kieu K" first="K." last="Kieu">K. Kieu</name><name sortKey="Mehravar, S" sort="Mehravar, S" uniqKey="Mehravar S" first="S." last="Mehravar">S. Mehravar</name><name sortKey="Norwood, R A" sort="Norwood, R A" uniqKey="Norwood R" first="R. A." last="Norwood">R. A. Norwood</name><name sortKey="Peyghambarian, N" sort="Peyghambarian, N" uniqKey="Peyghambarian N" first="N." last="Peyghambarian">N. Peyghambarian</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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