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Sequence-specific and hydrolytic scission of DNA and RNA by lanthanide complex-oligoDNA hybrids.

Identifieur interne : 000C39 ( Ncbi/Merge ); précédent : 000C38; suivant : 000C40

Sequence-specific and hydrolytic scission of DNA and RNA by lanthanide complex-oligoDNA hybrids.

Auteurs : M. Komiyama

Source :

Journal of biochemistry [ 0021-924X ] ; 1995.

RBID : pubmed:8576074

Descripteurs français

KwdFr :
- ADN (analyse), ARN (analyse), Analyse de séquence, Animaux, Données de séquences moléculaires, Désoxyribonucléases (synthèse chimique), Humains, Hydrolyse, Ribonucléases (synthèse chimique), Séquence nucléotidique, Terres rares.
MESH :
- analyse : ADN, ARN.
- synthèse chimique : Désoxyribonucléases, Ribonucléases.
- Analyse de séquence, Animaux, Données de séquences moléculaires, Humains, Hydrolyse, Séquence nucléotidique, Terres rares.

English descriptors

KwdEn :
- Animals, Base Sequence, DNA (analysis), Deoxyribonucleases (chemical synthesis), Humans, Hydrolysis, Metals, Rare Earth, Molecular Sequence Data, RNA (analysis), Ribonucleases (chemical synthesis), Sequence Analysis.
MESH :
- chemical , analysis : DNA, RNA.
- chemical , chemical synthesis : Deoxyribonucleases, Ribonucleases.
- Animals, Base Sequence, Humans, Hydrolysis, Metals, Rare Earth, Molecular Sequence Data, Sequence Analysis.

Abstract

Totally synthetic and sequence-specific nucleases and ribonucleases, which hydrolyze DNAs and RNAs selectively at target sites, have been prepared. Lanthanide ions, which efficiently hydrolyze the phosphodiester linkages in nucleic acids, are attached to the 5'-end of synthetic DNA oligomers (sequence-recognizing sites) by use of an iminodiacetate ligand. Under physiological conditions, the hybrids selectively hydrolyze substrate DNA or RNA at the 3'-side of the sequence which is complementary with the DNA oligomers in the hybrids. The cerium(IV) ion is the most active as to DNA scission, whereas the europium(III), thulium(III), and lutetium(III) ions are the most effective for RNA scission. All the scissions proceed totally via hydrolysis of the phosphodiester linkages, in the same way as the scissions by natural nucleases and ribonucleases do. The artificial enzymes, which show far greater sequence-specificities than natural ones, exhibit high potential for application to molecular biology, biotechnology, and therapy.

PubMed: 8576074

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pubmed:8576074

Le document en format XML

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<affiliation><nlm:affiliation>Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo.</nlm:affiliation>
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<term>Données de séquences moléculaires</term>
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<front><div type="abstract" xml:lang="en">Totally synthetic and sequence-specific nucleases and ribonucleases, which hydrolyze DNAs and RNAs selectively at target sites, have been prepared. Lanthanide ions, which efficiently hydrolyze the phosphodiester linkages in nucleic acids, are attached to the 5'-end of synthetic DNA oligomers (sequence-recognizing sites) by use of an iminodiacetate ligand. Under physiological conditions, the hybrids selectively hydrolyze substrate DNA or RNA at the 3'-side of the sequence which is complementary with the DNA oligomers in the hybrids. The cerium(IV) ion is the most active as to DNA scission, whereas the europium(III), thulium(III), and lutetium(III) ions are the most effective for RNA scission. All the scissions proceed totally via hydrolysis of the phosphodiester linkages, in the same way as the scissions by natural nucleases and ribonucleases do. The artificial enzymes, which show far greater sequence-specificities than natural ones, exhibit high potential for application to molecular biology, biotechnology, and therapy.</div>
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<Abstract><AbstractText>Totally synthetic and sequence-specific nucleases and ribonucleases, which hydrolyze DNAs and RNAs selectively at target sites, have been prepared. Lanthanide ions, which efficiently hydrolyze the phosphodiester linkages in nucleic acids, are attached to the 5'-end of synthetic DNA oligomers (sequence-recognizing sites) by use of an iminodiacetate ligand. Under physiological conditions, the hybrids selectively hydrolyze substrate DNA or RNA at the 3'-side of the sequence which is complementary with the DNA oligomers in the hybrids. The cerium(IV) ion is the most active as to DNA scission, whereas the europium(III), thulium(III), and lutetium(III) ions are the most effective for RNA scission. All the scissions proceed totally via hydrolysis of the phosphodiester linkages, in the same way as the scissions by natural nucleases and ribonucleases do. The artificial enzymes, which show far greater sequence-specificities than natural ones, exhibit high potential for application to molecular biology, biotechnology, and therapy.</AbstractText>
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Sequence-specific and hydrolytic scission of DNA and RNA by lanthanide complex-oligoDNA hybrids.

Sequence-specific and hydrolytic scission of DNA and RNA by lanthanide complex-oligoDNA hybrids.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki