Metal-Containing Polystyrene Beads as Standards for Mass Cytometry
Identifieur interne : 000375 ( Ncbi/Merge ); précédent : 000374; suivant : 000376Metal-Containing Polystyrene Beads as Standards for Mass Cytometry
Auteurs : Ahmed I. Abdelrahman ; Olga Ornatsky ; Dmitry Bandura ; Vladimir Baranov ; Robert Kinach ; Sheng Dai ; Stuart C. Thickett ; Scott Tanner ; Mitchell A. WinnikSource :
- Journal of analytical atomic spectrometry [ 0267-9477 ] ; 2010.
Abstract
We examine the suitability of metal-containing polystyrene beads for the calibration of a mass cytometer instrument, a single particle analyser based on an inductively coupled plasma ion source and a time of flight mass spectrometer. These metal-containing beads are also verified for their use as internal standards for this instrument. These beads were synthesized by multiple-stage dispersion polymerization with acrylic acid as a comonomer. Acrylic acid acts as a ligand to anchor the metal ions within the interior of the beads. Mass cytometry enabled the bead-by-bead measurement of the metal-content and determination of the metal-content distribution. Beads synthesized by dispersion polymerization that involved three stages were shown to have narrower bead-to-bead variation in their lanthanide content than beads synthesized by 2-stage dispersion polymerization. The beads exhibited insignificant release of their lanthanide content to aqueous solutions of different pHs over a period of six months. When mixed with KG1a or U937 cell lines, metal-containing polymer beads were shown not to affect the mass cytometry response to the metal content of element-tagged antibodies specifically attached to these cells.
Url:
DOI: 10.1039/b921770c
PubMed: 20390041
PubMed Central: 2852877
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000230
- to stream Pmc, to step Curation: 000230
- to stream Pmc, to step Checkpoint: 000290
Links to Exploration step
PMC:2852877Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Metal-Containing Polystyrene Beads as Standards for Mass Cytometry</title>
<author><name sortKey="Abdelrahman, Ahmed I" sort="Abdelrahman, Ahmed I" uniqKey="Abdelrahman A" first="Ahmed I." last="Abdelrahman">Ahmed I. Abdelrahman</name>
</author>
<author><name sortKey="Ornatsky, Olga" sort="Ornatsky, Olga" uniqKey="Ornatsky O" first="Olga" last="Ornatsky">Olga Ornatsky</name>
</author>
<author><name sortKey="Bandura, Dmitry" sort="Bandura, Dmitry" uniqKey="Bandura D" first="Dmitry" last="Bandura">Dmitry Bandura</name>
</author>
<author><name sortKey="Baranov, Vladimir" sort="Baranov, Vladimir" uniqKey="Baranov V" first="Vladimir" last="Baranov">Vladimir Baranov</name>
</author>
<author><name sortKey="Kinach, Robert" sort="Kinach, Robert" uniqKey="Kinach R" first="Robert" last="Kinach">Robert Kinach</name>
</author>
<author><name sortKey="Dai, Sheng" sort="Dai, Sheng" uniqKey="Dai S" first="Sheng" last="Dai">Sheng Dai</name>
</author>
<author><name sortKey="Thickett, Stuart C" sort="Thickett, Stuart C" uniqKey="Thickett S" first="Stuart C." last="Thickett">Stuart C. Thickett</name>
</author>
<author><name sortKey="Tanner, Scott" sort="Tanner, Scott" uniqKey="Tanner S" first="Scott" last="Tanner">Scott Tanner</name>
</author>
<author><name sortKey="Winnik, Mitchell A" sort="Winnik, Mitchell A" uniqKey="Winnik M" first="Mitchell A." last="Winnik">Mitchell A. Winnik</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">20390041</idno>
<idno type="pmc">2852877</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2852877</idno>
<idno type="RBID">PMC:2852877</idno>
<idno type="doi">10.1039/b921770c</idno>
<date when="2010">2010</date>
<idno type="wicri:Area/Pmc/Corpus">000230</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000230</idno>
<idno type="wicri:Area/Pmc/Curation">000230</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000230</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000290</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000290</idno>
<idno type="wicri:Area/Ncbi/Merge">000375</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Metal-Containing Polystyrene Beads as Standards for Mass Cytometry</title>
<author><name sortKey="Abdelrahman, Ahmed I" sort="Abdelrahman, Ahmed I" uniqKey="Abdelrahman A" first="Ahmed I." last="Abdelrahman">Ahmed I. Abdelrahman</name>
</author>
<author><name sortKey="Ornatsky, Olga" sort="Ornatsky, Olga" uniqKey="Ornatsky O" first="Olga" last="Ornatsky">Olga Ornatsky</name>
</author>
<author><name sortKey="Bandura, Dmitry" sort="Bandura, Dmitry" uniqKey="Bandura D" first="Dmitry" last="Bandura">Dmitry Bandura</name>
</author>
<author><name sortKey="Baranov, Vladimir" sort="Baranov, Vladimir" uniqKey="Baranov V" first="Vladimir" last="Baranov">Vladimir Baranov</name>
</author>
<author><name sortKey="Kinach, Robert" sort="Kinach, Robert" uniqKey="Kinach R" first="Robert" last="Kinach">Robert Kinach</name>
</author>
<author><name sortKey="Dai, Sheng" sort="Dai, Sheng" uniqKey="Dai S" first="Sheng" last="Dai">Sheng Dai</name>
</author>
<author><name sortKey="Thickett, Stuart C" sort="Thickett, Stuart C" uniqKey="Thickett S" first="Stuart C." last="Thickett">Stuart C. Thickett</name>
</author>
<author><name sortKey="Tanner, Scott" sort="Tanner, Scott" uniqKey="Tanner S" first="Scott" last="Tanner">Scott Tanner</name>
</author>
<author><name sortKey="Winnik, Mitchell A" sort="Winnik, Mitchell A" uniqKey="Winnik M" first="Mitchell A." last="Winnik">Mitchell A. Winnik</name>
</author>
</analytic>
<series><title level="j">Journal of analytical atomic spectrometry</title>
<idno type="ISSN">0267-9477</idno>
<imprint><date when="2010">2010</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p id="P1">We examine the suitability of metal-containing polystyrene beads for the calibration of a mass cytometer instrument, a single particle analyser based on an inductively coupled plasma ion source and a time of flight mass spectrometer. These metal-containing beads are also verified for their use as internal standards for this instrument. These beads were synthesized by multiple-stage dispersion polymerization with acrylic acid as a comonomer. Acrylic acid acts as a ligand to anchor the metal ions within the interior of the beads. Mass cytometry enabled the bead-by-bead measurement of the metal-content and determination of the metal-content distribution. Beads synthesized by dispersion polymerization that involved three stages were shown to have narrower bead-to-bead variation in their lanthanide content than beads synthesized by 2-stage dispersion polymerization. The beads exhibited insignificant release of their lanthanide content to aqueous solutions of different pHs over a period of six months. When mixed with KG1a or U937 cell lines, metal-containing polymer beads were shown not to affect the mass cytometry response to the metal content of element-tagged antibodies specifically attached to these cells.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article" xml:lang="EN"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">8608254</journal-id>
<journal-id journal-id-type="pubmed-jr-id">34523</journal-id>
<journal-id journal-id-type="nlm-ta">J Anal At Spectrom</journal-id>
<journal-title>Journal of analytical atomic spectrometry</journal-title>
<issn pub-type="ppub">0267-9477</issn>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">20390041</article-id>
<article-id pub-id-type="pmc">2852877</article-id>
<article-id pub-id-type="doi">10.1039/b921770c</article-id>
<article-id pub-id-type="manuscript">NIHMS178717</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Metal-Containing Polystyrene Beads as Standards for Mass Cytometry</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Abdelrahman</surname>
<given-names>Ahmed I.</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Ornatsky</surname>
<given-names>Olga</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Bandura</surname>
<given-names>Dmitry</given-names>
</name>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Baranov</surname>
<given-names>Vladimir</given-names>
</name>
<email>vladimir.baranov@utoronto.ca</email>
</contrib>
<contrib contrib-type="author"><name><surname>Kinach</surname>
<given-names>Robert</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Dai</surname>
<given-names>Sheng</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Thickett</surname>
<given-names>Stuart C.</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Tanner</surname>
<given-names>Scott</given-names>
</name>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Winnik</surname>
<given-names>Mitchell A.</given-names>
</name>
<email>mwinnik@chem.utoronto.ca</email>
</contrib>
<aff id="A1">Department of Chemistry, University of Toronto, 80 St George Street Toronto ON M5S3H6, Canada</aff>
</contrib-group>
<pub-date pub-type="nihms-submitted"><day>22</day>
<month>2</month>
<year>2010</year>
</pub-date>
<pub-date pub-type="ppub"><year>2010</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>1</day>
<month>1</month>
<year>2011</year>
</pub-date>
<volume>25</volume>
<issue>3</issue>
<fpage>260</fpage>
<lpage>268</lpage>
<abstract><p id="P1">We examine the suitability of metal-containing polystyrene beads for the calibration of a mass cytometer instrument, a single particle analyser based on an inductively coupled plasma ion source and a time of flight mass spectrometer. These metal-containing beads are also verified for their use as internal standards for this instrument. These beads were synthesized by multiple-stage dispersion polymerization with acrylic acid as a comonomer. Acrylic acid acts as a ligand to anchor the metal ions within the interior of the beads. Mass cytometry enabled the bead-by-bead measurement of the metal-content and determination of the metal-content distribution. Beads synthesized by dispersion polymerization that involved three stages were shown to have narrower bead-to-bead variation in their lanthanide content than beads synthesized by 2-stage dispersion polymerization. The beads exhibited insignificant release of their lanthanide content to aqueous solutions of different pHs over a period of six months. When mixed with KG1a or U937 cell lines, metal-containing polymer beads were shown not to affect the mass cytometry response to the metal content of element-tagged antibodies specifically attached to these cells.</p>
</abstract>
<contract-num rid="GM1">R01 GM076127-04
||GM</contract-num>
<contract-sponsor id="GM1">National Institute of General Medical Sciences : NIGMS</contract-sponsor>
</article-meta>
</front>
</pmc>
<affiliations><list></list>
<tree><noCountry><name sortKey="Abdelrahman, Ahmed I" sort="Abdelrahman, Ahmed I" uniqKey="Abdelrahman A" first="Ahmed I." last="Abdelrahman">Ahmed I. Abdelrahman</name>
<name sortKey="Bandura, Dmitry" sort="Bandura, Dmitry" uniqKey="Bandura D" first="Dmitry" last="Bandura">Dmitry Bandura</name>
<name sortKey="Baranov, Vladimir" sort="Baranov, Vladimir" uniqKey="Baranov V" first="Vladimir" last="Baranov">Vladimir Baranov</name>
<name sortKey="Dai, Sheng" sort="Dai, Sheng" uniqKey="Dai S" first="Sheng" last="Dai">Sheng Dai</name>
<name sortKey="Kinach, Robert" sort="Kinach, Robert" uniqKey="Kinach R" first="Robert" last="Kinach">Robert Kinach</name>
<name sortKey="Ornatsky, Olga" sort="Ornatsky, Olga" uniqKey="Ornatsky O" first="Olga" last="Ornatsky">Olga Ornatsky</name>
<name sortKey="Tanner, Scott" sort="Tanner, Scott" uniqKey="Tanner S" first="Scott" last="Tanner">Scott Tanner</name>
<name sortKey="Thickett, Stuart C" sort="Thickett, Stuart C" uniqKey="Thickett S" first="Stuart C." last="Thickett">Stuart C. Thickett</name>
<name sortKey="Winnik, Mitchell A" sort="Winnik, Mitchell A" uniqKey="Winnik M" first="Mitchell A." last="Winnik">Mitchell A. Winnik</name>
</noCountry>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Terre/explor/ThuliumV1/Data/Ncbi/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000375 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 000375 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Terre |area= ThuliumV1 |flux= Ncbi |étape= Merge |type= RBID |clé= PMC:2852877 |texte= Metal-Containing Polystyrene Beads as Standards for Mass Cytometry }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i -Sk "pubmed:20390041" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd \ | NlmPubMed2Wicri -a ThuliumV1
This area was generated with Dilib version V0.6.21. |