Novel type of bicellar disks from a mixture of DMPC and DMPE-DTPA with complexed lanthanides.
Identifieur interne : 000373 ( Ncbi/Merge ); précédent : 000372; suivant : 000374Novel type of bicellar disks from a mixture of DMPC and DMPE-DTPA with complexed lanthanides.
Auteurs : Paul Beck [Suisse] ; Marianne Liebi ; Joachim Kohlbrecher ; Takashi Ishikawa ; Heinz Rüegger ; Peter Fischer ; Peter Walde ; Erich WindhabSource :
- Langmuir : the ACS journal of surfaces and colloids [ 1520-5827 ] ; 2010.
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Abstract
We report on the formation of bicelles from a mixture of dimyristoylphosphatidylcholine (DMPC) and the chelator-lipid dimyristoylphosphatidylethanolamine-diethylenetriaminepentaacetate (DMPE-DTPA) with complexed lanthanides, either thulium (Tm(3+)) or lanthanum (La(3+)). The two phospholipids used have the same acyl-chain length but differ in headgroup size and chemical structure. The total lipid concentration was 15 mM, and the molar ratio of DMPC to DMPE-DTPA was 4:1. The system was studied with small angle neutron scattering (SANS) in a magnetic field, cryo-transmission electron microscopy (cryo-TEM), and (31)P NMR spectroscopy. We found that, after appropriate preparation steps, that is, extrusion through a polycarbonate membrane followed by a cooling step, monodisperse small unilamellar disks (flat cylinders called bicelles) are formed. They have a radius of 20 nm and a bilayer thickness of about 4 nm and are stable in the investigated temperature range of 2.5-30 degrees C. Fitting of SANS data with a form factor for partly aligned flat cylinders shows that the bicelles are slightly orientable in a magnetic field of 8 T if DMPE-DTPA is complexed with Tm(3+).
DOI: 10.1021/la903806a
PubMed: 20384368
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sortKey="Ishikawa, Takashi" sort="Ishikawa, Takashi" uniqKey="Ishikawa T" first="Takashi" last="Ishikawa">Takashi Ishikawa</name></author><author><name sortKey="Ruegger, Heinz" sort="Ruegger, Heinz" uniqKey="Ruegger H" first="Heinz" last="Rüegger">Heinz Rüegger</name></author><author><name sortKey="Fischer, Peter" sort="Fischer, Peter" uniqKey="Fischer P" first="Peter" last="Fischer">Peter Fischer</name></author><author><name sortKey="Walde, Peter" sort="Walde, Peter" uniqKey="Walde P" first="Peter" last="Walde">Peter Walde</name></author><author><name sortKey="Windhab, Erich" sort="Windhab, Erich" uniqKey="Windhab E" first="Erich" last="Windhab">Erich Windhab</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="doi">10.1021/la903806a</idno><idno type="RBID">pubmed:20384368</idno><idno type="pmid">20384368</idno><idno type="wicri:Area/PubMed/Corpus">000490</idno><idno type="wicri:explorRef" wicri:stream="PubMed" 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sort="Windhab, Erich" uniqKey="Windhab E" first="Erich" last="Windhab">Erich Windhab</name></author></analytic><series><title level="j">Langmuir : the ACS journal of surfaces and colloids</title><idno type="eISSN">1520-5827</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cryoelectron Microscopy</term><term>Dimyristoylphosphatidylcholine (chemistry)</term><term>Lanthanoid Series Elements (chemistry)</term><term>Magnetic Resonance Spectroscopy</term><term>Micelles</term><term>Microscopy, Electron, Transmission</term><term>Models, Theoretical</term><term>Pentetic Acid (chemistry)</term><term>Phosphatidylethanolamines (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acide pentétique ()</term><term>Cryomicroscopie électronique</term><term>Dimyristoylphosphatidylcholine ()</term><term>Lanthanides ()</term><term>Micelles</term><term>Microscopie électronique à transmission</term><term>Modèles théoriques</term><term>Phosphatidyléthanolamine ()</term><term>Spectroscopie par résonance magnétique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Dimyristoylphosphatidylcholine</term><term>Lanthanoid Series Elements</term><term>Pentetic Acid</term><term>Phosphatidylethanolamines</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cryoelectron Microscopy</term><term>Magnetic Resonance Spectroscopy</term><term>Micelles</term><term>Microscopy, Electron, Transmission</term><term>Models, Theoretical</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Acide pentétique</term><term>Cryomicroscopie électronique</term><term>Dimyristoylphosphatidylcholine</term><term>Lanthanides</term><term>Micelles</term><term>Microscopie électronique à transmission</term><term>Modèles 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The two phospholipids used have the same acyl-chain length but differ in headgroup size and chemical structure. The total lipid concentration was 15 mM, and the molar ratio of DMPC to DMPE-DTPA was 4:1. The system was studied with small angle neutron scattering (SANS) in a magnetic field, cryo-transmission electron microscopy (cryo-TEM), and (31)P NMR spectroscopy. We found that, after appropriate preparation steps, that is, extrusion through a polycarbonate membrane followed by a cooling step, monodisperse small unilamellar disks (flat cylinders called bicelles) are formed. They have a radius of 20 nm and a bilayer thickness of about 4 nm and are stable in the investigated temperature range of 2.5-30 degrees C. Fitting of SANS data with a form factor for partly aligned flat cylinders shows that the bicelles are slightly orientable in a magnetic field of 8 T if DMPE-DTPA is complexed with Tm(3+).</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">20384368</PMID><DateCreated><Year>2010</Year><Month>04</Month><Day>13</Day></DateCreated><DateCompleted><Year>2010</Year><Month>07</Month><Day>01</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1520-5827</ISSN><JournalIssue CitedMedium="Internet"><Volume>26</Volume><Issue>8</Issue><PubDate><Year>2010</Year><Month>Apr</Month><Day>20</Day></PubDate></JournalIssue><Title>Langmuir : the ACS journal of surfaces and colloids</Title><ISOAbbreviation>Langmuir</ISOAbbreviation></Journal><ArticleTitle>Novel type of bicellar disks from a mixture of DMPC and DMPE-DTPA with complexed lanthanides.</ArticleTitle><Pagination><MedlinePgn>5382-7</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/la903806a</ELocationID><Abstract><AbstractText>We report on the formation of bicelles from a mixture of dimyristoylphosphatidylcholine (DMPC) and the chelator-lipid dimyristoylphosphatidylethanolamine-diethylenetriaminepentaacetate (DMPE-DTPA) with complexed lanthanides, either thulium (Tm(3+)) or lanthanum (La(3+)). The two phospholipids used have the same acyl-chain length but differ in headgroup size and chemical structure. The total lipid concentration was 15 mM, and the molar ratio of DMPC to DMPE-DTPA was 4:1. The system was studied with small angle neutron scattering (SANS) in a magnetic field, cryo-transmission electron microscopy (cryo-TEM), and (31)P NMR spectroscopy. We found that, after appropriate preparation steps, that is, extrusion through a polycarbonate membrane followed by a cooling step, monodisperse small unilamellar disks (flat cylinders called bicelles) are formed. They have a radius of 20 nm and a bilayer thickness of about 4 nm and are stable in the investigated temperature range of 2.5-30 degrees C. Fitting of SANS data with a form factor for partly aligned flat cylinders shows that the bicelles are slightly orientable in a magnetic field of 8 T if DMPE-DTPA is complexed with Tm(3+).</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Beck</LastName><ForeName>Paul</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Laboratory of Food Process Engineering, ETH Zurich, Schmelzbergstrasse 9, 8092 Zurich, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liebi</LastName><ForeName>Marianne</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Kohlbrecher</LastName><ForeName>Joachim</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Ishikawa</LastName><ForeName>Takashi</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Rüegger</LastName><ForeName>Heinz</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Fischer</LastName><ForeName>Peter</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Walde</LastName><ForeName>Peter</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Windhab</LastName><ForeName>Erich</ForeName><Initials>E</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Langmuir</MedlineTA><NlmUniqueID>9882736</NlmUniqueID><ISSNLinking>0743-7463</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D028581">Lanthanoid Series Elements</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008823">Micelles</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010714">Phosphatidylethanolamines</NameOfSubstance></Chemical><Chemical><RegistryNumber>20255-95-2</RegistryNumber><NameOfSubstance UI="C018525">1,2-dimyristoylphosphatidylethanolamine</NameOfSubstance></Chemical><Chemical><RegistryNumber>7A314HQM0I</RegistryNumber><NameOfSubstance UI="D004369">Pentetic Acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>U86ZGC74V5</RegistryNumber><NameOfSubstance UI="D004134">Dimyristoylphosphatidylcholine</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N" UI="D020285">Cryoelectron Microscopy</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D004134">Dimyristoylphosphatidylcholine</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000737">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D028581">Lanthanoid Series Elements</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000737">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D009682">Magnetic Resonance Spectroscopy</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y" UI="D008823">Micelles</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D046529">Microscopy, Electron, Transmission</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D008962">Models, Theoretical</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D004369">Pentetic Acid</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000737">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D010714">Phosphatidylethanolamines</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000737">chemistry</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>4</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>4</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>7</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="doi">10.1021/la903806a</ArticleId><ArticleId IdType="pubmed">20384368</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Suisse</li></country></list><tree><noCountry><name sortKey="Fischer, Peter" sort="Fischer, Peter" uniqKey="Fischer P" first="Peter" last="Fischer">Peter Fischer</name><name sortKey="Ishikawa, Takashi" sort="Ishikawa, Takashi" uniqKey="Ishikawa T" first="Takashi" last="Ishikawa">Takashi Ishikawa</name><name sortKey="Kohlbrecher, Joachim" sort="Kohlbrecher, Joachim" uniqKey="Kohlbrecher J" first="Joachim" last="Kohlbrecher">Joachim Kohlbrecher</name><name sortKey="Liebi, Marianne" sort="Liebi, Marianne" uniqKey="Liebi M" first="Marianne" last="Liebi">Marianne Liebi</name><name sortKey="Ruegger, Heinz" sort="Ruegger, Heinz" uniqKey="Ruegger H" first="Heinz" last="Rüegger">Heinz Rüegger</name><name sortKey="Walde, Peter" sort="Walde, Peter" uniqKey="Walde P" first="Peter" last="Walde">Peter Walde</name><name sortKey="Windhab, Erich" sort="Windhab, Erich" uniqKey="Windhab E" first="Erich" last="Windhab">Erich Windhab</name></noCountry><country name="Suisse"><noRegion><name sortKey="Beck, Paul" sort="Beck, Paul" uniqKey="Beck P" first="Paul" last="Beck">Paul Beck</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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