(13) C-TmDOTA as versatile thermometer compound for solid-state NMR of hydrated lipid bilayer membranes.
Identifieur interne : 000A62 ( Ncbi/Curation ); précédent : 000A61; suivant : 000A63(13) C-TmDOTA as versatile thermometer compound for solid-state NMR of hydrated lipid bilayer membranes.
Auteurs : Yuichi Umegawa [Japon] ; Yuya Tanaka [Japon] ; Matsumori Nobuaki [Japon] ; Michio Murata [Japon]Source :
- Magnetic resonance in chemistry : MRC [ 1097-458X ] ; 2016.
Abstract
Recent advances in solid-state nuclear magnetic resonance (NMR) techniques, such as magic angle spinning and high-power decoupling, have dramatically increased the sensitivity and resolution of NMR. However, these NMR techniques generate extra heat, causing a temperature difference between the sample in the rotor and the variable temperature gas. This extra heating is a particularly crucial problem for hydrated lipid membrane samples. Thus, to develop an NMR thermometer that is suitable for hydrated lipid samples, thulium-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (TmDOTA) was synthesized and labeled with (13) C (i.e., (13) C-TmDOTA) to increase the NMR sensitivity. The complex was mixed with a hydrated lipid membrane, and the system was subjected to solid-state NMR and differential scanning calorimetric analyses. The physical properties of the lipid bilayer and the quality of the NMR spectra of the membrane were negligibly affected by the presence of (13) C-TmDOTA, and the (13) C chemical shift of the complex exhibited a large-temperature dependence. The results demonstrated that (13) C-TmDOTA could be successfully used as a thermometer to accurately monitor temperature changes induced by (1) H decoupling pulses and/or by magic angle spinning and the temperature distribution of the sample inside the rotor. Thus, (13) C-TmDOTA was shown to be a versatile thermometer for hydrated lipid assemblies. Copyright © 2015 John Wiley & Sons, Ltd.
DOI: 10.1002/mrc.4371
PubMed: 26460094
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first="Yuya" last="Tanaka">Yuya Tanaka</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka, 560-0043, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Chemistry, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka, 560-0043</wicri:regionArea><wicri:noRegion>560-0043</wicri:noRegion></affiliation></author><author><name sortKey="Nobuaki, Matsumori" sort="Nobuaki, Matsumori" uniqKey="Nobuaki M" first="Matsumori" last="Nobuaki">Matsumori Nobuaki</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka, 560-0043, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Chemistry, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka, 560-0043</wicri:regionArea><wicri:noRegion>560-0043</wicri:noRegion></affiliation></author><author><name sortKey="Murata, Michio" sort="Murata, Michio" uniqKey="Murata M" first="Michio" last="Murata">Michio Murata</name><affiliation wicri:level="1"><nlm:affiliation>JST ERATO, Lipid Active Structure Project, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka, 560-0043, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>JST ERATO, Lipid Active Structure Project, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka, 560-0043</wicri:regionArea><wicri:noRegion>560-0043</wicri:noRegion></affiliation></author></analytic><series><title level="j">Magnetic resonance in chemistry : MRC</title><idno type="eISSN">1097-458X</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Recent advances in solid-state nuclear magnetic resonance (NMR) techniques, such as magic angle spinning and high-power decoupling, have dramatically increased the sensitivity and resolution of NMR. However, these NMR techniques generate extra heat, causing a temperature difference between the sample in the rotor and the variable temperature gas. This extra heating is a particularly crucial problem for hydrated lipid membrane samples. Thus, to develop an NMR thermometer that is suitable for hydrated lipid samples, thulium-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (TmDOTA) was synthesized and labeled with (13) C (i.e., (13) C-TmDOTA) to increase the NMR sensitivity. The complex was mixed with a hydrated lipid membrane, and the system was subjected to solid-state NMR and differential scanning calorimetric analyses. The physical properties of the lipid bilayer and the quality of the NMR spectra of the membrane were negligibly affected by the presence of (13) C-TmDOTA, and the (13) C chemical shift of the complex exhibited a large-temperature dependence. The results demonstrated that (13) C-TmDOTA could be successfully used as a thermometer to accurately monitor temperature changes induced by (1) H decoupling pulses and/or by magic angle spinning and the temperature distribution of the sample inside the rotor. Thus, (13) C-TmDOTA was shown to be a versatile thermometer for hydrated lipid assemblies. Copyright © 2015 John Wiley & Sons, Ltd.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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