23Na NMR shift reagents enhance cardiac staircase effect in isolated perfused rat hearts.
Identifieur interne : 000002 ( Ncbi/Curation ); précédent : 000001; suivant : 00000323Na NMR shift reagents enhance cardiac staircase effect in isolated perfused rat hearts.
Auteurs : T. Simor [États-Unis] ; T. L Ránd ; A. Szöllösy ; B. Gaszner ; S B Digerness ; G A ElgavishSource :
- NMR in biomedicine [ 0952-3480 ] ; 1999.
Descripteurs français
- KwdFr :
- Acide édétique (analogues et dérivés), Acide édétique (pharmacologie), Animaux, Calcium (métabolisme), Coeur (), Coeur (physiologie), Composés organiques du phosphore (pharmacologie), Composés organométalliques (pharmacologie), Dysprosium (pharmacologie), Fonction ventriculaire gauche (), Indicateurs et réactifs, Myocarde (métabolisme), Mâle, Perfusion, Polyphosphates (pharmacologie), Pression ventriculaire (), Radio-isotopes du sodium, Rat Sprague-Dawley, Rats, Rythme cardiaque (), Résonance magnétique nucléaire biomoléculaire.
- MESH :
- analogues et dérivés : Acide édétique.
- métabolisme : Calcium, Myocarde.
- pharmacologie : Acide édétique, Composés organiques du phosphore, Composés organométalliques, Dysprosium, Polyphosphates.
- physiologie : Coeur.
- Animaux, Coeur, Fonction ventriculaire gauche, Indicateurs et réactifs, Mâle, Perfusion, Pression ventriculaire, Radio-isotopes du sodium, Rat Sprague-Dawley, Rats, Rythme cardiaque, Résonance magnétique nucléaire biomoléculaire.
English descriptors
- KwdEn :
- Animals, Calcium (metabolism), Dysprosium (pharmacology), Edetic Acid (analogs & derivatives), Edetic Acid (pharmacology), Heart (drug effects), Heart (physiology), Heart Rate (drug effects), Indicators and Reagents, Male, Myocardium (metabolism), Nuclear Magnetic Resonance, Biomolecular, Organometallic Compounds (pharmacology), Organophosphorus Compounds (pharmacology), Perfusion, Polyphosphates (pharmacology), Rats, Rats, Sprague-Dawley, Sodium Radioisotopes, Ventricular Function, Left (drug effects), Ventricular Pressure (drug effects).
- MESH :
- chemical , analogs & derivatives : Edetic Acid.
- chemical , metabolism : Calcium.
- chemical , pharmacology : Dysprosium, Edetic Acid, Organometallic Compounds, Organophosphorus Compounds, Polyphosphates.
- drug effects : Heart, Heart Rate, Ventricular Function, Left, Ventricular Pressure.
- metabolism : Myocardium.
- physiology : Heart.
- Animals, Indicators and Reagents, Male, Nuclear Magnetic Resonance, Biomolecular, Perfusion, Rats, Rats, Sprague-Dawley, Sodium Radioisotopes.
Abstract
The effects of the currently used (23)Na NMR shift reagents, dysprosium bis-triphosphate [Dy(PPP)(2)], dysprosium triethylenetriamine hexaacetate [Dy(TTHA)] and thulium 1,4,7, 10-tetraazacyclododecane-N,N',N",N"'-tetra(methylenephosphonate) [Tm(DOTP)] were studied in the rat heart cardiac staircase model. Rat hearts were perfused with low or normal extracellular free calcium ([Ca(o)](f)). At low [Ca(o)](f) (0.34 +/- 0.05 mM), hearts were perfused with Dy(PPP)(2) (group I), Dy(TTHA) (group II) or no shift reagent (group III), while at normal [Ca(o)](f) (1.25 +/- 0.15 mM), hearts were perfused with Tm(DOTP) (group IV), Dy(TTHA) (group V) or no shift reagent (group VI). Left ventricular developed pressure (LVDP) values in group I were significantly higher than in groups II and III (p < 0.01), while no significant differences were found between groups II and III. LVDP values in group IV were significantly higher than in groups V and VI (p < 0.05), while the LVDP values in groups V and VI were almost identical. Also, a positive correlation between pacing rate and intracellular sodium ([Na(i)]) was evident. The [Na(i)] values at high [Ca(o)](f) were significantly lower than at low [Ca(o)](f) at each pacing level (p <0.01), indicating a negative correlation between [Na(i)] and [Ca(o)](f). No statistical differences were found in [Na(i)] between groups I vs II and IV vs V, showing that determination of [Na(i)] is not affected by any of these shift reagents. Thus the different LVDP responses in groups I vs II and IV vs V were not mirrored in [Na(i)] changes. We hypothesize that a direct, sarcolemmal Ca-Dy(PPP)(2)-, or Ca-Tm(DOTP)-induced positive inotropic effect could be responsible for these Na(i)-independent LVDP increases in groups I and IV.
PubMed: 10484815
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pubmed:10484815Le document en format XML
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Szöllösy</name></author><author><name sortKey="Gaszner, B" sort="Gaszner, B" uniqKey="Gaszner B" first="B" last="Gaszner">B. Gaszner</name></author><author><name sortKey="Digerness, S B" sort="Digerness, S B" uniqKey="Digerness S" first="S B" last="Digerness">S B Digerness</name></author><author><name sortKey="Elgavish, G A" sort="Elgavish, G A" uniqKey="Elgavish G" first="G A" last="Elgavish">G A Elgavish</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1999">1999</date><idno type="RBID">pubmed:10484815</idno><idno type="pmid">10484815</idno><idno type="wicri:Area/PubMed/Corpus">000739</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000739</idno><idno type="wicri:Area/PubMed/Curation">000739</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000739</idno><idno type="wicri:Area/PubMed/Checkpoint">000739</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000739</idno><idno type="wicri:Area/Ncbi/Merge">000002</idno><idno type="wicri:Area/Ncbi/Curation">000002</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">23Na NMR shift reagents enhance cardiac staircase effect in isolated perfused rat hearts.</title><author><name sortKey="Simor, T" sort="Simor, T" uniqKey="Simor T" first="T" last="Simor">T. Simor</name><affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry and Molecular Genetics, and the Center for Nuclear Imaging Research, AL 35294, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry and Molecular Genetics, and the Center for Nuclear Imaging Research, AL 35294</wicri:regionArea><placeName><region type="state">Alabama</region></placeName></affiliation></author><author><name sortKey="L Rand, T" sort="L Rand, T" uniqKey="L Rand T" first="T" last="L Ránd">T. L Ránd</name></author><author><name sortKey="Szollosy, A" sort="Szollosy, A" uniqKey="Szollosy A" first="A" last="Szöllösy">A. Szöllösy</name></author><author><name sortKey="Gaszner, B" sort="Gaszner, B" uniqKey="Gaszner B" first="B" last="Gaszner">B. Gaszner</name></author><author><name sortKey="Digerness, S B" sort="Digerness, S B" uniqKey="Digerness S" first="S B" last="Digerness">S B Digerness</name></author><author><name sortKey="Elgavish, G A" sort="Elgavish, G A" uniqKey="Elgavish G" first="G A" last="Elgavish">G A Elgavish</name></author></analytic><series><title level="j">NMR in biomedicine</title><idno type="ISSN">0952-3480</idno><imprint><date when="1999" type="published">1999</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Calcium (metabolism)</term><term>Dysprosium (pharmacology)</term><term>Edetic Acid (analogs & derivatives)</term><term>Edetic Acid (pharmacology)</term><term>Heart (drug effects)</term><term>Heart (physiology)</term><term>Heart Rate (drug effects)</term><term>Indicators and Reagents</term><term>Male</term><term>Myocardium (metabolism)</term><term>Nuclear Magnetic Resonance, Biomolecular</term><term>Organometallic Compounds (pharmacology)</term><term>Organophosphorus Compounds (pharmacology)</term><term>Perfusion</term><term>Polyphosphates (pharmacology)</term><term>Rats</term><term>Rats, Sprague-Dawley</term><term>Sodium Radioisotopes</term><term>Ventricular Function, Left (drug effects)</term><term>Ventricular Pressure (drug effects)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acide édétique (analogues et dérivés)</term><term>Acide édétique (pharmacologie)</term><term>Animaux</term><term>Calcium (métabolisme)</term><term>Coeur ()</term><term>Coeur (physiologie)</term><term>Composés organiques du phosphore (pharmacologie)</term><term>Composés organométalliques (pharmacologie)</term><term>Dysprosium (pharmacologie)</term><term>Fonction ventriculaire gauche ()</term><term>Indicateurs et réactifs</term><term>Myocarde (métabolisme)</term><term>Mâle</term><term>Perfusion</term><term>Polyphosphates (pharmacologie)</term><term>Pression ventriculaire ()</term><term>Radio-isotopes du sodium</term><term>Rat Sprague-Dawley</term><term>Rats</term><term>Rythme cardiaque ()</term><term>Résonance magnétique nucléaire biomoléculaire</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Edetic Acid</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Calcium</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Dysprosium</term><term>Edetic Acid</term><term>Organometallic Compounds</term><term>Organophosphorus Compounds</term><term>Polyphosphates</term></keywords><keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Acide édétique</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Heart</term><term>Heart Rate</term><term>Ventricular Function, Left</term><term>Ventricular Pressure</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Myocardium</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Calcium</term><term>Myocarde</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Acide édétique</term><term>Composés organiques du phosphore</term><term>Composés organométalliques</term><term>Dysprosium</term><term>Polyphosphates</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Coeur</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Heart</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Indicators and Reagents</term><term>Male</term><term>Nuclear Magnetic Resonance, Biomolecular</term><term>Perfusion</term><term>Rats</term><term>Rats, Sprague-Dawley</term><term>Sodium Radioisotopes</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Coeur</term><term>Fonction ventriculaire gauche</term><term>Indicateurs et réactifs</term><term>Mâle</term><term>Perfusion</term><term>Pression ventriculaire</term><term>Radio-isotopes du sodium</term><term>Rat Sprague-Dawley</term><term>Rats</term><term>Rythme cardiaque</term><term>Résonance magnétique nucléaire biomoléculaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The effects of the currently used (23)Na NMR shift reagents, dysprosium bis-triphosphate [Dy(PPP)(2)], dysprosium triethylenetriamine hexaacetate [Dy(TTHA)] and thulium 1,4,7, 10-tetraazacyclododecane-N,N',N",N"'-tetra(methylenephosphonate) [Tm(DOTP)] were studied in the rat heart cardiac staircase model. Rat hearts were perfused with low or normal extracellular free calcium ([Ca(o)](f)). At low [Ca(o)](f) (0.34 +/- 0.05 mM), hearts were perfused with Dy(PPP)(2) (group I), Dy(TTHA) (group II) or no shift reagent (group III), while at normal [Ca(o)](f) (1.25 +/- 0.15 mM), hearts were perfused with Tm(DOTP) (group IV), Dy(TTHA) (group V) or no shift reagent (group VI). Left ventricular developed pressure (LVDP) values in group I were significantly higher than in groups II and III (p < 0.01), while no significant differences were found between groups II and III. LVDP values in group IV were significantly higher than in groups V and VI (p < 0.05), while the LVDP values in groups V and VI were almost identical. Also, a positive correlation between pacing rate and intracellular sodium ([Na(i)]) was evident. The [Na(i)] values at high [Ca(o)](f) were significantly lower than at low [Ca(o)](f) at each pacing level (p <0.01), indicating a negative correlation between [Na(i)] and [Ca(o)](f). No statistical differences were found in [Na(i)] between groups I vs II and IV vs V, showing that determination of [Na(i)] is not affected by any of these shift reagents. Thus the different LVDP responses in groups I vs II and IV vs V were not mirrored in [Na(i)] changes. We hypothesize that a direct, sarcolemmal Ca-Dy(PPP)(2)-, or Ca-Tm(DOTP)-induced positive inotropic effect could be responsible for these Na(i)-independent LVDP increases in groups I and IV.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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