NMR observation of gramicidin A' in phosphatidylcholine vesicles.
Identifieur interne : 000C07 ( Ncbi/Checkpoint ); précédent : 000C06; suivant : 000C08NMR observation of gramicidin A' in phosphatidylcholine vesicles.
Auteurs : G W Feigenson ; P R Meers ; P B KingsleySource :
- Biochimica et biophysica acta [ 0006-3002 ] ; 1977.
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Abstract
Dimyristoyl phosphatidylcholine was prepared with perdeuterated hydrocarbon chains and sonicated into bilayer vesicles together with gramicidin A'. The 1H NMR resonance from the tryptophan residues in the gramicidin has a linewidth of approximately 80 Hz, indicating significant local mobility for these residues. Paramagnetic lanthanides added to the aqueous medium cause a chemical shift of this signal indicating that some of the tryptophans may be located in the interfacial region of the bilayer.
PubMed: 72565
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