ThuliumV1, Main, Exploration, bibRecordById, PMC:93179

In Vivo 23Na Nuclear Magnetic Resonance Study of Maintenance of a Sodium Gradient in the Ruminal Bacterium Fibrobacter succinogenes S85

Identifieur interne : 001F74 ( Main/Exploration ); précédent : 001F73; suivant : 001F75

In Vivo 23Na Nuclear Magnetic Resonance Study of Maintenance of a Sodium Gradient in the Ruminal Bacterium Fibrobacter succinogenes S85

Auteurs : Véronique Schwaab ; Christelle Matheron ; Anne-Marie Delort ; Geneviève Gaudet ; Evelyne Forano

Source :

Applied and Environmental Microbiology [ 0099-2240 ] ; 2001.

RBID : PMC:93179

Descripteurs français

KwdFr :
- Animaux, Bactéries anaérobies (physiologie), Chlorure de sodium (métabolisme), Composés organiques du phosphore (métabolisme), Composés organométalliques (métabolisme), Isotopes du sodium (métabolisme), Rumen (microbiologie), Spectroscopie par résonance magnétique ().
MESH :
- microbiologie : Rumen.
- métabolisme : Chlorure de sodium, Composés organiques du phosphore, Composés organométalliques, Isotopes du sodium.
- physiologie : Bactéries anaérobies.
- Animaux, Spectroscopie par résonance magnétique.

English descriptors

KwdEn :
- Animals, Bacteria, Anaerobic (physiology), Magnetic Resonance Spectroscopy (methods), Organometallic Compounds (metabolism), Organophosphorus Compounds (metabolism), Rumen (microbiology), Sodium Chloride (metabolism), Sodium Isotopes (metabolism).
MESH :
- chemical , metabolism : Organometallic Compounds, Organophosphorus Compounds, Sodium Chloride, Sodium Isotopes.
- methods : Magnetic Resonance Spectroscopy.
- microbiology : Rumen.
- physiology : Bacteria, Anaerobic.
- Animals.

Abstract

Sodium gradients (ΔpNa) were measured in resting cells of Fibrobacter succinogenes by in vivo ²³Na nuclear magnetic resonance using Tm(DOTP)⁵⁻ [thulium(III) 1,4,7,10-tetraazacyclododecane-N′,N′′,N′′′-tetramethylenephosphonate] as the shift reagent. This bacterium was able to maintain a ΔpNa of −55 to −40 mV for extracellular sodium concentrations ranging from 30 to 200 mM. Depletion of Na⁺ ions during the washing steps led to irreversible damage (modification of glucose metabolism and inability to maintain a sodium gradient).

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC93179

DOI: 10.1128/AEM.67.9.4390-4392.2001
PubMed: 11526055
PubMed Central: 93179

Affiliations:

Le document en format XML

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Na Nuclear Magnetic Resonance Study of Maintenance  of a Sodium Gradient in the Ruminal Bacterium  <italic>Fibrobacter succinogenes</italic>
 S85</title>
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 S85</title>
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<front><div type="abstract" xml:lang="en"><p>Sodium gradients (ΔpNa) were measured in resting cells of <italic>Fibrobacter succinogenes</italic>
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′,<italic>N</italic>
′′,<italic>N</italic>
′′′-tetramethylenephosphonate] as the shift reagent. This bacterium was able to maintain a ΔpNa of −55 to −40 mV for extracellular sodium concentrations ranging from 30 to 200 mM. Depletion of Na<sup>+</sup>
 ions during the washing steps led to irreversible damage (modification of glucose metabolism and inability to maintain a sodium gradient).</p>
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In Vivo 23Na Nuclear Magnetic Resonance Study of Maintenance of a Sodium Gradient in the Ruminal Bacterium Fibrobacter succinogenes S85

In Vivo 23Na Nuclear Magnetic Resonance Study of Maintenance of a Sodium Gradient in the Ruminal Bacterium Fibrobacter succinogenes S85

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

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