Subwavelength imaging through ion-beam-induced upconversion
Identifieur interne : 000091 ( Main/Exploration ); précédent : 000090; suivant : 000092Subwavelength imaging through ion-beam-induced upconversion
Auteurs : Zhaohong Mi [Singapour] ; Yuhai Zhang [Singapour] ; Sudheer Kumar Vanga [Singapour] ; Ce-Belle Chen [Singapour] ; Hong Qi Tan [Singapour] ; Frank Watt [Singapour] ; Xiaogang Liu [Singapour, République populaire de Chine] ; Andrew A. Bettiol [Singapour]Source :
- Nature Communications [ 2041-1723 ] ; 2015.
Abstract
The combination of an optical microscope and a luminescent probe plays a pivotal role in biological imaging because it allows for probing subcellular structures. However, the optical resolutions are largely constrained by Abbe's diffraction limit, and the common dye probes often suffer from photobleaching. Here we present a new method for subwavelength imaging by combining lanthanide-doped upconversion nanocrystals with the ionoluminescence imaging technique. We experimentally observed that the ion beam can be used as a new form of excitation source to induce photon upconversion in lanthanide-doped nanocrystals. This approach enables luminescence imaging and simultaneous mapping of cellular structures with a spatial resolution of sub-30 nm.
Url:
DOI: 10.1038/ncomms9832
PubMed: 26560858
PubMed Central: 4660043
Affiliations:
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Le document en format XML
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<front><div type="abstract" xml:lang="en"><p>The combination of an optical microscope and a luminescent probe plays a pivotal role in biological imaging because it allows for probing subcellular structures. However, the optical resolutions are largely constrained by Abbe's diffraction limit, and the common dye probes often suffer from photobleaching. Here we present a new method for subwavelength imaging by combining lanthanide-doped upconversion nanocrystals with the ionoluminescence imaging technique. We experimentally observed that the ion beam can be used as a new form of excitation source to induce photon upconversion in lanthanide-doped nanocrystals. This approach enables luminescence imaging and simultaneous mapping of cellular structures with a spatial resolution of sub-30 nm.</p>
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<name sortKey="Liu, Xiaogang" sort="Liu, Xiaogang" uniqKey="Liu X" first="Xiaogang" last="Liu">Xiaogang Liu</name>
<name sortKey="Liu, Xiaogang" sort="Liu, Xiaogang" uniqKey="Liu X" first="Xiaogang" last="Liu">Xiaogang Liu</name>
<name sortKey="Tan, Hong Qi" sort="Tan, Hong Qi" uniqKey="Tan H" first="Hong Qi" last="Tan">Hong Qi Tan</name>
<name sortKey="Vanga, Sudheer Kumar" sort="Vanga, Sudheer Kumar" uniqKey="Vanga S" first="Sudheer Kumar" last="Vanga">Sudheer Kumar Vanga</name>
<name sortKey="Watt, Frank" sort="Watt, Frank" uniqKey="Watt F" first="Frank" last="Watt">Frank Watt</name>
<name sortKey="Zhang, Yuhai" sort="Zhang, Yuhai" uniqKey="Zhang Y" first="Yuhai" last="Zhang">Yuhai Zhang</name>
</country>
<country name="République populaire de Chine"><noRegion><name sortKey="Liu, Xiaogang" sort="Liu, Xiaogang" uniqKey="Liu X" first="Xiaogang" last="Liu">Xiaogang Liu</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
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