ThuliumV1, Main, Exploration, bibRecordById, PMC:411546

Conformation of gramicidin A channel in phospholipid vesicles: a 13C and 19F nuclear magnetic resonance study.

Identifieur interne : 004995 ( Main/Exploration ); précédent : 004994; suivant : 004996

Conformation of gramicidin A channel in phospholipid vesicles: a 13C and 19F nuclear magnetic resonance study.

Auteurs : S. Weinstein ; B A Wallace ; E R Blout ; J S Morrow ; W. Veatch

Source :

Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 1979.

RBID : PMC:411546

Descripteurs français

KwdFr :
- Canaux ioniques (), Conformation des protéines, Double couche lipidique, Fluor, Gramicidine, Isotopes du carbone, Lipides membranaires, Manganèse (pharmacologie), Marqueurs de spin, Phosphatidylcholines, Spectroscopie par résonance magnétique, Thulium (pharmacologie).
MESH :
- pharmacologie : Manganèse, Thulium.
- Canaux ioniques, Conformation des protéines, Double couche lipidique, Fluor, Gramicidine, Isotopes du carbone, Lipides membranaires, Marqueurs de spin, Phosphatidylcholines, Spectroscopie par résonance magnétique.

English descriptors

KwdEn :
- Carbon Isotopes, Fluorine, Gramicidin, Ion Channels (drug effects), Lipid Bilayers, Magnetic Resonance Spectroscopy, Manganese (pharmacology), Membrane Lipids, Phosphatidylcholines, Protein Conformation, Spin Labels, Thulium (pharmacology).
MESH :
- chemical , drug effects : Ion Channels.
- chemical , pharmacology : Manganese, Thulium.
- chemical : Carbon Isotopes, Fluorine, Gramicidin, Lipid Bilayers, Membrane Lipids, Phosphatidylcholines, Spin Labels.
- Magnetic Resonance Spectroscopy, Protein Conformation.

Abstract

We have determined the conformation of the channel-forming polypeptide antibiotic gramicidin A in phosphatidylcholine vesicles by using 13C and 19F NMR spectroscopy. The models previously proposed for the conformation of the dimer channel differ in the surface localization of the NH2 and COOH termini. We have incorporated specific 13C and 19F nuclei at both the NH2, and COOH termini of gramicidin and have used 13C and 19F chemical shifts and spin lattice relaxation time measurements to determine the accessibility of these labels to three paramagnetic NMR probes--two in aqueous solution and one attached to the phosphatidylcholine fatty acid chain9 all of our results indicate that the COOH terminus of gramicidin in the channel is located near the surface of the membrane and the NH2 terminus is buried deep within the lipid bilayer. These findings strongly favor an NH2-terminal to NH2-terminal helical dimer as the major conformation for the gramicidin channel in phosphatidylcholine vesicles.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC411546

PubMed: 92025
PubMed Central: 411546

Affiliations:

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Conformation of gramicidin A channel in phospholipid vesicles: a 13C and 19F nuclear magnetic resonance study.</title>
<author><name sortKey="Weinstein, S" sort="Weinstein, S" uniqKey="Weinstein S" first="S" last="Weinstein">S. Weinstein</name>
</author>
<author><name sortKey="Wallace, B A" sort="Wallace, B A" uniqKey="Wallace B" first="B A" last="Wallace">B A Wallace</name>
</author>
<author><name sortKey="Blout, E R" sort="Blout, E R" uniqKey="Blout E" first="E R" last="Blout">E R Blout</name>
</author>
<author><name sortKey="Morrow, J S" sort="Morrow, J S" uniqKey="Morrow J" first="J S" last="Morrow">J S Morrow</name>
</author>
<author><name sortKey="Veatch, W" sort="Veatch, W" uniqKey="Veatch W" first="W" last="Veatch">W. Veatch</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">92025</idno>
<idno type="pmc">411546</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC411546</idno>
<idno type="RBID">PMC:411546</idno>
<date when="1979">1979</date>
<idno type="wicri:Area/Pmc/Corpus">000184</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000184</idno>
<idno type="wicri:Area/Pmc/Curation">000184</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000184</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000414</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000414</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="wicri:Area/PubMed/Corpus">000860</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000860</idno>
<idno type="wicri:Area/PubMed/Curation">000860</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000860</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000860</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000860</idno>
<idno type="wicri:Area/Ncbi/Merge">000C56</idno>
<idno type="wicri:Area/Ncbi/Curation">000C56</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000C56</idno>
<idno type="wicri:doubleKey">0027-8424:1979:Weinstein S:conformation:of:gramicidin</idno>
<idno type="wicri:Area/Main/Merge">004E19</idno>
<idno type="wicri:Area/Main/Curation">004995</idno>
<idno type="wicri:Area/Main/Exploration">004995</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Conformation of gramicidin A channel in phospholipid vesicles: a 13C and 19F nuclear magnetic resonance study.</title>
<author><name sortKey="Weinstein, S" sort="Weinstein, S" uniqKey="Weinstein S" first="S" last="Weinstein">S. Weinstein</name>
</author>
<author><name sortKey="Wallace, B A" sort="Wallace, B A" uniqKey="Wallace B" first="B A" last="Wallace">B A Wallace</name>
</author>
<author><name sortKey="Blout, E R" sort="Blout, E R" uniqKey="Blout E" first="E R" last="Blout">E R Blout</name>
</author>
<author><name sortKey="Morrow, J S" sort="Morrow, J S" uniqKey="Morrow J" first="J S" last="Morrow">J S Morrow</name>
</author>
<author><name sortKey="Veatch, W" sort="Veatch, W" uniqKey="Veatch W" first="W" last="Veatch">W. Veatch</name>
</author>
</analytic>
<series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title>
<idno type="ISSN">0027-8424</idno>
<idno type="eISSN">1091-6490</idno>
<imprint><date when="1979">1979</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Carbon Isotopes</term>
<term>Fluorine</term>
<term>Gramicidin</term>
<term>Ion Channels (drug effects)</term>
<term>Lipid Bilayers</term>
<term>Magnetic Resonance Spectroscopy</term>
<term>Manganese (pharmacology)</term>
<term>Membrane Lipids</term>
<term>Phosphatidylcholines</term>
<term>Protein Conformation</term>
<term>Spin Labels</term>
<term>Thulium (pharmacology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Canaux ioniques ()</term>
<term>Conformation des protéines</term>
<term>Double couche lipidique</term>
<term>Fluor</term>
<term>Gramicidine</term>
<term>Isotopes du carbone</term>
<term>Lipides membranaires</term>
<term>Manganèse (pharmacologie)</term>
<term>Marqueurs de spin</term>
<term>Phosphatidylcholines</term>
<term>Spectroscopie par résonance magnétique</term>
<term>Thulium (pharmacologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="drug effects" xml:lang="en"><term>Ion Channels</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Manganese</term>
<term>Thulium</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Carbon Isotopes</term>
<term>Fluorine</term>
<term>Gramicidin</term>
<term>Lipid Bilayers</term>
<term>Membrane Lipids</term>
<term>Phosphatidylcholines</term>
<term>Spin Labels</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Manganèse</term>
<term>Thulium</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Magnetic Resonance Spectroscopy</term>
<term>Protein Conformation</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Canaux ioniques</term>
<term>Conformation des protéines</term>
<term>Double couche lipidique</term>
<term>Fluor</term>
<term>Gramicidine</term>
<term>Isotopes du carbone</term>
<term>Lipides membranaires</term>
<term>Marqueurs de spin</term>
<term>Phosphatidylcholines</term>
<term>Spectroscopie par résonance magnétique</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>We have determined the conformation of the channel-forming polypeptide antibiotic gramicidin A in phosphatidylcholine vesicles by using 13C and 19F NMR spectroscopy. The models previously proposed for the conformation of the dimer channel differ in the surface localization of the NH2 and COOH termini. We have incorporated specific 13C and 19F nuclei at both the NH2, and COOH termini of gramicidin and have used 13C and 19F chemical shifts and spin lattice relaxation time measurements to determine the accessibility of these labels to three paramagnetic NMR probes--two in aqueous solution and one attached to the phosphatidylcholine fatty acid chain9 all of our results indicate that the COOH terminus of gramicidin in the channel is located near the surface of the membrane and the NH2 terminus is buried deep within the lipid bilayer. These findings strongly favor an NH2-terminal to NH2-terminal helical dimer as the major conformation for the gramicidin channel in phosphatidylcholine vesicles.</p>
</div>
</front>
</TEI>
<affiliations><list></list>
<tree><noCountry><name sortKey="Blout, E R" sort="Blout, E R" uniqKey="Blout E" first="E R" last="Blout">E R Blout</name>
<name sortKey="Morrow, J S" sort="Morrow, J S" uniqKey="Morrow J" first="J S" last="Morrow">J S Morrow</name>
<name sortKey="Veatch, W" sort="Veatch, W" uniqKey="Veatch W" first="W" last="Veatch">W. Veatch</name>
<name sortKey="Wallace, B A" sort="Wallace, B A" uniqKey="Wallace B" first="B A" last="Wallace">B A Wallace</name>
<name sortKey="Weinstein, S" sort="Weinstein, S" uniqKey="Weinstein S" first="S" last="Weinstein">S. Weinstein</name>
</noCountry>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Terre/explor/ThuliumV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004995 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004995 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Terre
   |area=    ThuliumV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     PMC:411546
   |texte=   Conformation of gramicidin A channel in phospholipid vesicles: a 13C and 19F nuclear magnetic resonance study.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:92025" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a ThuliumV1

This area was generated with Dilib version V0.6.21.
Data generation: Thu May 12 08:27:09 2016. Site generation: Thu Mar 7 22:33:44 2024

Serveur d'exploration sur le thulium

Conformation of gramicidin A channel in phospholipid vesicles: a 13C and 19F nuclear magnetic resonance study.

Conformation of gramicidin A channel in phospholipid vesicles: a 13C and 19F nuclear magnetic resonance study.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration sur le thulium
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.