A new cDNA encoding a xyloglucan endo -transglycosylase-related polypeptide ( AtXTR8 ) preferentially expressed in seedling, root and stem of Arabidopsis thaliana
Identifieur interne : 002C47 ( Main/Exploration ); précédent : 002C46; suivant : 002C48A new cDNA encoding a xyloglucan endo -transglycosylase-related polypeptide ( AtXTR8 ) preferentially expressed in seedling, root and stem of Arabidopsis thaliana
Auteurs : Dominique Aubert [France] ; Michel Herzog [France]Source :
- Plant Science [ 0168-9452 ] ; 1996.
Descripteurs français
- Wicri :
- topic : Plant.
English descriptors
- KwdEn :
- Amino, Amino acid identity, Amino acid sequence, Amino acid sequences, Arabidopsis, Arubidopsis, Atxtr8, Atxtr8 cdna, Atxtr8 gene, Atxtr8 gene expression, Atxtr8 mrna accumulation, Atxtr8 sequence, Atxtr8 signal, Atxtr8 transcripts, Auxin, Auxin treatment, Bestfit program, Biol, Cdna, Cell wall, Cellulose microfibrils, Clone, Complex cdna probe, Cotyledon, Cucumber hypocotyls, Different expression patterns, Differential screen, Enzymatic activity, Exogenous gibberellins, Expression patterns, Flower buds, Fresh medium, Gapc, Gene, Gene family, Germinated nasturtium seeds, Germinating, Germinating seeds, Germination, Germination process, Gibberellin, Gibberellin biosynthetic inhibitor uniconazol, High levels, Imbibition, Imbibition medium, Lower extent, Maize roots, Mrna, Msar medium, Mutant, Nasturtium, Nasturtium tmnxgl, Novel class, Other hand, Other members, Peptide, Physiol, Plant cell, Plant growth, Plant physiol, Protein family, Protein sequence, Protein sequences, Putative, Seed germination, Seedling, Single fragment, Stringent conditions, Tmnxgl, Tmnxgl protein, Various plant species, Vegetative tissues, Wild type, Wild type seedlings, Wild type seeds, Xyloglucan, Xyloglucan activity, Xyloglucan endotransglycosylase, Xyloglucan molecule, Xyloglucan polymers, Young seedlings.
- Teeft :
- Amino, Amino acid identity, Amino acid sequence, Amino acid sequences, Arabidopsis, Arubidopsis, Atxtr8, Atxtr8 cdna, Atxtr8 gene, Atxtr8 gene expression, Atxtr8 mrna accumulation, Atxtr8 sequence, Atxtr8 signal, Atxtr8 transcripts, Auxin, Auxin treatment, Bestfit program, Biol, Cdna, Cell wall, Cellulose microfibrils, Clone, Complex cdna probe, Cotyledon, Cucumber hypocotyls, Different expression patterns, Differential screen, Enzymatic activity, Exogenous gibberellins, Expression patterns, Flower buds, Fresh medium, Gapc, Gene, Gene family, Germinated nasturtium seeds, Germinating, Germinating seeds, Germination, Germination process, Gibberellin, Gibberellin biosynthetic inhibitor uniconazol, High levels, Imbibition, Imbibition medium, Lower extent, Maize roots, Mrna, Msar medium, Mutant, Nasturtium, Nasturtium tmnxgl, Novel class, Other hand, Other members, Peptide, Physiol, Plant cell, Plant growth, Plant physiol, Protein family, Protein sequence, Protein sequences, Putative, Seed germination, Seedling, Single fragment, Stringent conditions, Tmnxgl, Tmnxgl protein, Various plant species, Vegetative tissues, Wild type, Wild type seedlings, Wild type seeds, Xyloglucan, Xyloglucan activity, Xyloglucan endotransglycosylase, Xyloglucan molecule, Xyloglucan polymers, Young seedlings.
Abstract
Abstract: A new cDNA clone (AtXTR8) encoding a xyloglucan endo-transglycosylase (XET)-related protein was isolated from an Arabidopsis thaliana germinating seed cDNA library, using a differential screen. The predicted amino acid sequence displays only 38 to 42% amino acid identity with other members of the Arabidopsis XTR (XET-related) protein family but shows 64% identity to a xyloglucan endo-transglycosylase enzyme (TmNXG1) expressed in nasturtium (Tropaeolum majus) germinating seeds. The AtXTR8 transcipts are expressed in germinating seeds as early as 24 h after imbibition with highest level of accumulation reached at about 72 h. In adult plants, transcripts are mainly detected in roots and to a lower extent in inflorescence stems. During imbibition of the severely gibberellin-deficient gal-3 mutant seeds, transcripts are detected only if exogenous gibberellins are supplied in the medium. suggesting that gibberellins are directly or indirectly required for AtXTR8 gene expression. Unlike other XET genes such as TCH4, EXT and XTR3 which are rapidly activated by auxins in wild type Arabidopsis, the steady state level of AtXTR8 transcripts is not markedly increased by auxin treatment of 6-day-old seedlings. Phylogenetic analysis confirms that the XET and XTR protein sequences so far identified in various plant species split in three groups and Arabidopsis AtXTR8, which enzymatic activity has not been assessed, belongs to the least related group III.
Url:
DOI: 10.1016/S0168-9452(96)04522-0
Affiliations:
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Le document en format XML
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<front><div type="abstract" xml:lang="en">Abstract: A new cDNA clone (AtXTR8) encoding a xyloglucan endo-transglycosylase (XET)-related protein was isolated from an Arabidopsis thaliana germinating seed cDNA library, using a differential screen. The predicted amino acid sequence displays only 38 to 42% amino acid identity with other members of the Arabidopsis XTR (XET-related) protein family but shows 64% identity to a xyloglucan endo-transglycosylase enzyme (TmNXG1) expressed in nasturtium (Tropaeolum majus) germinating seeds. The AtXTR8 transcipts are expressed in germinating seeds as early as 24 h after imbibition with highest level of accumulation reached at about 72 h. In adult plants, transcripts are mainly detected in roots and to a lower extent in inflorescence stems. During imbibition of the severely gibberellin-deficient gal-3 mutant seeds, transcripts are detected only if exogenous gibberellins are supplied in the medium. suggesting that gibberellins are directly or indirectly required for AtXTR8 gene expression. Unlike other XET genes such as TCH4, EXT and XTR3 which are rapidly activated by auxins in wild type Arabidopsis, the steady state level of AtXTR8 transcripts is not markedly increased by auxin treatment of 6-day-old seedlings. Phylogenetic analysis confirms that the XET and XTR protein sequences so far identified in various plant species split in three groups and Arabidopsis AtXTR8, which enzymatic activity has not been assessed, belongs to the least related group III.</div>
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