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Magnetically Aligned Phospholipid Bilayers with Positive Ordering: A New Model Membrane System

Identifieur interne : 002779 ( Main/Exploration ); précédent : 002778; suivant : 002780

Magnetically Aligned Phospholipid Bilayers with Positive Ordering: A New Model Membrane System

Auteurs : R. Scott Prosser [États-Unis] ; Jimmy S. Hwang [États-Unis] ; Regitze R. Vold [États-Unis]

Source :

RBID : ISTEX:E23026121987B88C68DD0FA466BF719888E54859

Descripteurs français

English descriptors

Abstract

Abstract: A stable smectic phospholipid bilayer phase aligned with the director parallel to the magnetic field can be generated by the addition of certain trivalent paramagnetic lanthanide ions to a bicellar solution of dimyristoylphosphatidylcholine (DMPC) and dihexanoylphosphatidylcholine (DHPC) in water. Suitable lanthanide ions are those with positive anisotropy of their magnetic susceptibility, namely Eu3+, Er3+, Tm3+, and Yb3+. For samples doped with Tm3+, this phase extends over a wide range of Tm3+ concentrations (6–40mM) and temperatures (35–90°C) and appears to undergo a transition from a fluid nematic discotic to a fluid, but highly ordered, smectic phase at a temperature that depends on the thulium concentration. As a membrane mimetic, these new, positively ordered phospholipid phases have high potential for structural studies using a variety of techniques such as magnetic resonance (EMR and NMR), small-angle x-ray and neutron diffraction, as well as optical and infrared spectroscopy.

Url:
DOI: 10.1016/S0006-3495(98)77949-4


Affiliations:


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Le document en format XML

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<term>Acta</term>
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<term>Anisotropy</term>
<term>Bicellar</term>
<term>Bicellar samples</term>
<term>Bicelle</term>
<term>Bicelles</term>
<term>Bilayer</term>
<term>Bilayered</term>
<term>Bilayers</term>
<term>Biochemistry</term>
<term>Biochim</term>
<term>Biophys</term>
<term>Biophysical</term>
<term>Bleaney</term>
<term>Chem</term>
<term>Chemical shifts</term>
<term>Conformational changes</term>
<term>Deuterium</term>
<term>Deuterium spectra</term>
<term>Deuteron</term>
<term>Dhpc</term>
<term>Dimyristoylphosphatidylcholine (chemistry)</term>
<term>Discotic</term>
<term>Discotic nematic</term>
<term>Dmpc</term>
<term>Dmpc spectra</term>
<term>Headgroup</term>
<term>Ideal bicelle</term>
<term>Ion</term>
<term>Isotropic</term>
<term>Isotropic phase</term>
<term>Isotropic resonance</term>
<term>Lafleur</term>
<term>Lanthanide</term>
<term>Lanthanide ions</term>
<term>Lecithin</term>
<term>Lipid</term>
<term>Lipid Bilayers (chemistry)</term>
<term>Magnetic alignment</term>
<term>Magnetic anisotropy</term>
<term>Magnetic field</term>
<term>Magnetic fields</term>
<term>Magnetic properties</term>
<term>Magnetic resonance</term>
<term>Magnetic susceptibility</term>
<term>Magnetics</term>
<term>Mcgarvey</term>
<term>Membrane</term>
<term>Membrane proteins</term>
<term>Mesogenic unit</term>
<term>Metals, Rare Earth</term>
<term>Methylene</term>
<term>Methylene groups</term>
<term>Micelles</term>
<term>Model membranes</term>
<term>Models, Biological</term>
<term>Models, Molecular</term>
<term>Molecular Conformation</term>
<term>Molecular axis</term>
<term>Negative order</term>
<term>Nematic</term>
<term>Nuclear Magnetic Resonance, Biomolecular</term>
<term>Order parameter</term>
<term>Order parameters</term>
<term>Orientational</term>
<term>Orientational order</term>
<term>Other words</term>
<term>Paramagnetic ions</term>
<term>Paramagnetic shifts</term>
<term>Parameter</term>
<term>Peptide</term>
<term>Phospholipid</term>
<term>Phospholipid Ethers (chemistry)</term>
<term>Phospholipid bicelles</term>
<term>Phospholipid bilayers</term>
<term>Phospholipid membranes</term>
<term>Prestegard</term>
<term>Principal axis</term>
<term>Prosser</term>
<term>Quadrupolar</term>
<term>Quadrupolar splitting</term>
<term>Quadrupolar splittings</term>
<term>Realignment</term>
<term>Room temperature</term>
<term>Sample preparation</term>
<term>Sander</term>
<term>Schwonek</term>
<term>Seelig</term>
<term>Small amount</term>
<term>Smectic</term>
<term>Smectic phase</term>
<term>Spectrum</term>
<term>Splitting</term>
<term>Splittings</term>
<term>Structural studies</term>
<term>Susceptibility</term>
<term>Temperature dependence</term>
<term>Thermodynamics</term>
<term>Thulium</term>
<term>Trivalent</term>
<term>Trivalent ions</term>
<term>Trivalent lanthanide ions</term>
<term>Vold</term>
<term>Wide range</term>
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<keywords scheme="KwdFr" xml:lang="fr">
<term>Conformation moléculaire</term>
<term>Dimyristoylphosphatidylcholine ()</term>
<term>Double couche lipidique ()</term>
<term>Magnétisme</term>
<term>Micelles</term>
<term>Modèles biologiques</term>
<term>Modèles moléculaires</term>
<term>Résonance magnétique nucléaire biomoléculaire</term>
<term>Terres rares</term>
<term>Thermodynamique</term>
<term>Éther-phospholipides ()</term>
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<term>Dimyristoylphosphatidylcholine</term>
<term>Lipid Bilayers</term>
<term>Phospholipid Ethers</term>
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<keywords scheme="Teeft" xml:lang="en">
<term>Acta</term>
<term>Alkyl chains</term>
<term>Anisotropy</term>
<term>Bicellar</term>
<term>Bicellar samples</term>
<term>Bicelle</term>
<term>Bicelles</term>
<term>Bilayer</term>
<term>Bilayered</term>
<term>Bilayers</term>
<term>Biochemistry</term>
<term>Biochim</term>
<term>Biophys</term>
<term>Biophysical</term>
<term>Bleaney</term>
<term>Chem</term>
<term>Chemical shifts</term>
<term>Conformational changes</term>
<term>Deuterium</term>
<term>Deuterium spectra</term>
<term>Deuteron</term>
<term>Dhpc</term>
<term>Discotic</term>
<term>Discotic nematic</term>
<term>Dmpc</term>
<term>Dmpc spectra</term>
<term>Headgroup</term>
<term>Ideal bicelle</term>
<term>Ion</term>
<term>Isotropic</term>
<term>Isotropic phase</term>
<term>Isotropic resonance</term>
<term>Lafleur</term>
<term>Lanthanide</term>
<term>Lanthanide ions</term>
<term>Lecithin</term>
<term>Lipid</term>
<term>Magnetic alignment</term>
<term>Magnetic anisotropy</term>
<term>Magnetic field</term>
<term>Magnetic fields</term>
<term>Magnetic properties</term>
<term>Magnetic resonance</term>
<term>Magnetic susceptibility</term>
<term>Magnetics</term>
<term>Mcgarvey</term>
<term>Membrane</term>
<term>Membrane proteins</term>
<term>Mesogenic unit</term>
<term>Metals, Rare Earth</term>
<term>Methylene</term>
<term>Methylene groups</term>
<term>Micelles</term>
<term>Model membranes</term>
<term>Models, Biological</term>
<term>Models, Molecular</term>
<term>Molecular Conformation</term>
<term>Molecular axis</term>
<term>Negative order</term>
<term>Nematic</term>
<term>Nuclear Magnetic Resonance, Biomolecular</term>
<term>Order parameter</term>
<term>Order parameters</term>
<term>Orientational</term>
<term>Orientational order</term>
<term>Other words</term>
<term>Paramagnetic ions</term>
<term>Paramagnetic shifts</term>
<term>Parameter</term>
<term>Peptide</term>
<term>Phospholipid</term>
<term>Phospholipid bicelles</term>
<term>Phospholipid bilayers</term>
<term>Phospholipid membranes</term>
<term>Prestegard</term>
<term>Principal axis</term>
<term>Prosser</term>
<term>Quadrupolar</term>
<term>Quadrupolar splitting</term>
<term>Quadrupolar splittings</term>
<term>Realignment</term>
<term>Room temperature</term>
<term>Sample preparation</term>
<term>Sander</term>
<term>Schwonek</term>
<term>Seelig</term>
<term>Small amount</term>
<term>Smectic</term>
<term>Smectic phase</term>
<term>Spectrum</term>
<term>Splitting</term>
<term>Splittings</term>
<term>Structural studies</term>
<term>Susceptibility</term>
<term>Temperature dependence</term>
<term>Thermodynamics</term>
<term>Thulium</term>
<term>Trivalent</term>
<term>Trivalent ions</term>
<term>Trivalent lanthanide ions</term>
<term>Vold</term>
<term>Wide range</term>
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<term>Biochimie</term>
<term>Conformation moléculaire</term>
<term>Dimyristoylphosphatidylcholine</term>
<term>Double couche lipidique</term>
<term>Magnétisme</term>
<term>Micelles</term>
<term>Modèles biologiques</term>
<term>Modèles moléculaires</term>
<term>Résonance magnétique nucléaire biomoléculaire</term>
<term>Terres rares</term>
<term>Thermodynamique</term>
<term>Éther-phospholipides</term>
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<front>
<div type="abstract" xml:lang="en">Abstract: A stable smectic phospholipid bilayer phase aligned with the director parallel to the magnetic field can be generated by the addition of certain trivalent paramagnetic lanthanide ions to a bicellar solution of dimyristoylphosphatidylcholine (DMPC) and dihexanoylphosphatidylcholine (DHPC) in water. Suitable lanthanide ions are those with positive anisotropy of their magnetic susceptibility, namely Eu3+, Er3+, Tm3+, and Yb3+. For samples doped with Tm3+, this phase extends over a wide range of Tm3+ concentrations (6–40mM) and temperatures (35–90°C) and appears to undergo a transition from a fluid nematic discotic to a fluid, but highly ordered, smectic phase at a temperature that depends on the thulium concentration. As a membrane mimetic, these new, positively ordered phospholipid phases have high potential for structural studies using a variety of techniques such as magnetic resonance (EMR and NMR), small-angle x-ray and neutron diffraction, as well as optical and infrared spectroscopy.</div>
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