The Effects of the NMR Shift-reagents Dy(PPP)2, Dy(TTHA) and Tm(DOTP) on Developed Pressure in Isolated Perfused Rat Hearts. The Role of Shift-reagent Calcium Complexes
Identifieur interne : 002044 ( Main/Exploration ); précédent : 002043; suivant : 002045The Effects of the NMR Shift-reagents Dy(PPP)2, Dy(TTHA) and Tm(DOTP) on Developed Pressure in Isolated Perfused Rat Hearts. The Role of Shift-reagent Calcium Complexes
Auteurs : Balázs Gaszner [États-Unis] ; Tamás Simor [États-Unis] ; Gábor Hild [États-Unis] ; Gabriel A. Elgavish [États-Unis]Source :
- Journal of Molecular and Cellular Cardiology [ 0022-2828 ] ; 2001.
English descriptors
- KwdEn :
- Cacl2, Caot, Caot range, Cell cardiol, Chelate, Concentration range, Contractile, Contractile function, Control group, Direct inotropic effect, Dissociation constants, Dotp, Dycl3, Dysprosium, Excess ligand, Extracellular, Free dotp, Free dotp signal, Free ttha, Gaszner, Hepes, Hepes buffer, Hyperbolic function, Inotropic, Intracellular, Intracellular sodium, Inverse correlation, Isolated perfused rat hearts, Ligand, Lter membrane, Lvdp, Lvdp values, Lvdpmax, Magn, Magn reson, NMR shift-reagents, Perfusate, Perfused, Perfused hearts, Perfusion, Pore size, Positive inotropic effect, Positive inotropy, Positive modulators, Precipitation, Reagent, Reson, Same lvdpmax, Sarcolemmal calcium transporter, Shift reagent, Shift reagents, Shift-reagent calcium complexes., Spectroscopic titration method, Stock solution, Titration, Titration method, Ttha, Ttha ligand, Upper limit.
- Teeft :
- Cacl2, Caot, Caot range, Cell cardiol, Chelate, Concentration range, Contractile, Contractile function, Control group, Direct inotropic effect, Dissociation constants, Dotp, Dycl3, Dysprosium, Excess ligand, Extracellular, Free dotp, Free dotp signal, Free ttha, Gaszner, Hepes, Hepes buffer, Hyperbolic function, Inotropic, Intracellular, Intracellular sodium, Inverse correlation, Ligand, Lter membrane, Lvdp, Lvdp values, Lvdpmax, Magn, Magn reson, Perfusate, Perfused, Perfused hearts, Perfusion, Pore size, Positive inotropic effect, Precipitation, Reagent, Reson, Same lvdpmax, Sarcolemmal calcium transporter, Shift reagent, Shift reagents, Spectroscopic titration method, Stock solution, Titration, Titration method, Ttha, Ttha ligand, Upper limit.
Abstract
Abstract: The23Na NMR shift-reagent complexes (Dy(PPP)2, Dy(TTHA), and Tm(DOTP)) bind stoichiometric amounts of Ca2+. Thus, in perfused rat heart systems, a supplementation of Ca2+is required to maintain the requisite extracellular free calcium concentration ([Cao]f) and to approximate a physiological level of contractile function. The amount of reagent-bound Ca2+in a heart perfusate that contains a shift-reagent depends on: (1) Ca2+binding by excess ligand used during the preparation of the shift-reagent; and (2) the Ca2+binding affinity of the shift-reagent. To address point 1), we introduced a1H and31P NMR spectroscopic titration method to quantify directly the concentration of the excess ligand. We also used this method to minimize the amount of excess ligand (L) and thus the amount of Ca·L complex. To address point (2), we determined the stepwise Kd(μm) values of the Ca complexes of the three shift-reagents·: Dy(PPP)2, Kd1=0.09, Kd2=7.9; Dy(TTHA),Kd1 =10.66, Kd2=10.12; and Tm(DOTP), Kd1=0.502,Kd2 =4.98. The Kdvalues of the Ca complexes of the phosphonate and triphosphate based shift-reagents, Tm(DOTP) and Dy(PPP)2, respectively, are lower than those of the polyaminocarboxylate-based Dy(TTHA), indicating stronger Ca binding affinities for the former two types of complexes. We have also shown a positive correlation between [Cao]fand left ventricular developed pressure (LVDP) in perfused rat hearts. Dy(TTHA) has shown no effect on LVDP v[Cao]f. The LVDP values in the presence of the phosphonate and triphosphate based shift-reagents, however, were significantly higher than expected from the [Cao]flevels alone. Thus a positive inotropic effect, independent of [Cao]f, is evident in the presence of Tm(DOTP) or Dy(PPP)2.
Url:
DOI: 10.1006/jmcc.2001.1459
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001424
- to stream Istex, to step Curation: 001424
- to stream Istex, to step Checkpoint: 000952
- to stream Main, to step Merge: 002140
- to stream Main, to step Curation: 002044
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">The Effects of the NMR Shift-reagents Dy(PPP)2, Dy(TTHA) and Tm(DOTP) on Developed Pressure in Isolated Perfused Rat Hearts. The Role of Shift-reagent Calcium Complexes</title><author><name sortKey="Gaszner, Balazs" sort="Gaszner, Balazs" uniqKey="Gaszner B" first="Balázs" last="Gaszner">Balázs Gaszner</name></author><author><name sortKey="Simor, Tamas" sort="Simor, Tamas" uniqKey="Simor T" first="Tamás" last="Simor">Tamás Simor</name></author><author><name sortKey="Hild, Gabor" sort="Hild, Gabor" uniqKey="Hild G" first="Gábor" last="Hild">Gábor Hild</name></author><author><name sortKey="Elgavish, Gabriel A" sort="Elgavish, Gabriel A" uniqKey="Elgavish G" first="Gabriel A." last="Elgavish">Gabriel A. Elgavish</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:5B70B3D578596F3BC8D87F9255574C50DF49431D</idno><date when="2001" year="2001">2001</date><idno type="doi">10.1006/jmcc.2001.1459</idno><idno type="url">https://api.istex.fr/document/5B70B3D578596F3BC8D87F9255574C50DF49431D/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001424</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001424</idno><idno type="wicri:Area/Istex/Curation">001424</idno><idno type="wicri:Area/Istex/Checkpoint">000952</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000952</idno><idno type="wicri:doubleKey">0022-2828:2001:Gaszner B:the:effects:of</idno><idno type="wicri:Area/Main/Merge">002140</idno><idno type="wicri:Area/Main/Curation">002044</idno><idno type="wicri:Area/Main/Exploration">002044</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">The Effects of the NMR Shift-reagents Dy(PPP)2, Dy(TTHA) and Tm(DOTP) on Developed Pressure in Isolated Perfused Rat Hearts. The Role of Shift-reagent Calcium Complexes</title><author><name sortKey="Gaszner, Balazs" sort="Gaszner, Balazs" uniqKey="Gaszner B" first="Balázs" last="Gaszner">Balázs Gaszner</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry & Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL 35294</wicri:regionArea><placeName><region type="state">Alabama</region></placeName></affiliation></author><author><name sortKey="Simor, Tamas" sort="Simor, Tamas" uniqKey="Simor T" first="Tamás" last="Simor">Tamás Simor</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry & Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL 35294</wicri:regionArea><placeName><region type="state">Alabama</region></placeName></affiliation></author><author><name sortKey="Hild, Gabor" sort="Hild, Gabor" uniqKey="Hild G" first="Gábor" last="Hild">Gábor Hild</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry & Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL 35294</wicri:regionArea><placeName><region type="state">Alabama</region></placeName></affiliation></author><author><name sortKey="Elgavish, Gabriel A" sort="Elgavish, Gabriel A" uniqKey="Elgavish G" first="Gabriel A." last="Elgavish">Gabriel A. Elgavish</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry & Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL 35294</wicri:regionArea><placeName><region type="state">Alabama</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Molecular and Cellular Cardiology</title><title level="j" type="abbrev">YJMCC</title><idno type="ISSN">0022-2828</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2001">2001</date><biblScope unit="volume">33</biblScope><biblScope unit="issue">11</biblScope><biblScope unit="page" from="1945">1945</biblScope><biblScope unit="page" to="1956">1956</biblScope></imprint><idno type="ISSN">0022-2828</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-2828</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cacl2</term><term>Caot</term><term>Caot range</term><term>Cell cardiol</term><term>Chelate</term><term>Concentration range</term><term>Contractile</term><term>Contractile function</term><term>Control group</term><term>Direct inotropic effect</term><term>Dissociation constants</term><term>Dotp</term><term>Dycl3</term><term>Dysprosium</term><term>Excess ligand</term><term>Extracellular</term><term>Free dotp</term><term>Free dotp signal</term><term>Free ttha</term><term>Gaszner</term><term>Hepes</term><term>Hepes buffer</term><term>Hyperbolic function</term><term>Inotropic</term><term>Intracellular</term><term>Intracellular sodium</term><term>Inverse correlation</term><term>Isolated perfused rat hearts</term><term>Ligand</term><term>Lter membrane</term><term>Lvdp</term><term>Lvdp values</term><term>Lvdpmax</term><term>Magn</term><term>Magn reson</term><term>NMR shift-reagents</term><term>Perfusate</term><term>Perfused</term><term>Perfused hearts</term><term>Perfusion</term><term>Pore size</term><term>Positive inotropic effect</term><term>Positive inotropy</term><term>Positive modulators</term><term>Precipitation</term><term>Reagent</term><term>Reson</term><term>Same lvdpmax</term><term>Sarcolemmal calcium transporter</term><term>Shift reagent</term><term>Shift reagents</term><term>Shift-reagent calcium complexes.</term><term>Spectroscopic titration method</term><term>Stock solution</term><term>Titration</term><term>Titration method</term><term>Ttha</term><term>Ttha ligand</term><term>Upper limit</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Cacl2</term><term>Caot</term><term>Caot range</term><term>Cell cardiol</term><term>Chelate</term><term>Concentration range</term><term>Contractile</term><term>Contractile function</term><term>Control group</term><term>Direct inotropic effect</term><term>Dissociation constants</term><term>Dotp</term><term>Dycl3</term><term>Dysprosium</term><term>Excess ligand</term><term>Extracellular</term><term>Free dotp</term><term>Free dotp signal</term><term>Free ttha</term><term>Gaszner</term><term>Hepes</term><term>Hepes buffer</term><term>Hyperbolic function</term><term>Inotropic</term><term>Intracellular</term><term>Intracellular sodium</term><term>Inverse correlation</term><term>Ligand</term><term>Lter membrane</term><term>Lvdp</term><term>Lvdp values</term><term>Lvdpmax</term><term>Magn</term><term>Magn reson</term><term>Perfusate</term><term>Perfused</term><term>Perfused hearts</term><term>Perfusion</term><term>Pore size</term><term>Positive inotropic effect</term><term>Precipitation</term><term>Reagent</term><term>Reson</term><term>Same lvdpmax</term><term>Sarcolemmal calcium transporter</term><term>Shift reagent</term><term>Shift reagents</term><term>Spectroscopic titration method</term><term>Stock solution</term><term>Titration</term><term>Titration method</term><term>Ttha</term><term>Ttha ligand</term><term>Upper limit</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The23Na NMR shift-reagent complexes (Dy(PPP)2, Dy(TTHA), and Tm(DOTP)) bind stoichiometric amounts of Ca2+. Thus, in perfused rat heart systems, a supplementation of Ca2+is required to maintain the requisite extracellular free calcium concentration ([Cao]f) and to approximate a physiological level of contractile function. The amount of reagent-bound Ca2+in a heart perfusate that contains a shift-reagent depends on: (1) Ca2+binding by excess ligand used during the preparation of the shift-reagent; and (2) the Ca2+binding affinity of the shift-reagent. To address point 1), we introduced a1H and31P NMR spectroscopic titration method to quantify directly the concentration of the excess ligand. We also used this method to minimize the amount of excess ligand (L) and thus the amount of Ca·L complex. To address point (2), we determined the stepwise Kd(μm) values of the Ca complexes of the three shift-reagents·: Dy(PPP)2, Kd1=0.09, Kd2=7.9; Dy(TTHA),Kd1 =10.66, Kd2=10.12; and Tm(DOTP), Kd1=0.502,Kd2 =4.98. The Kdvalues of the Ca complexes of the phosphonate and triphosphate based shift-reagents, Tm(DOTP) and Dy(PPP)2, respectively, are lower than those of the polyaminocarboxylate-based Dy(TTHA), indicating stronger Ca binding affinities for the former two types of complexes. We have also shown a positive correlation between [Cao]fand left ventricular developed pressure (LVDP) in perfused rat hearts. Dy(TTHA) has shown no effect on LVDP v[Cao]f. The LVDP values in the presence of the phosphonate and triphosphate based shift-reagents, however, were significantly higher than expected from the [Cao]flevels alone. Thus a positive inotropic effect, independent of [Cao]f, is evident in the presence of Tm(DOTP) or Dy(PPP)2.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Alabama</li></region></list><tree><country name="États-Unis"><region name="Alabama"><name sortKey="Gaszner, Balazs" sort="Gaszner, Balazs" uniqKey="Gaszner B" first="Balázs" last="Gaszner">Balázs Gaszner</name></region><name sortKey="Elgavish, Gabriel A" sort="Elgavish, Gabriel A" uniqKey="Elgavish G" first="Gabriel A." last="Elgavish">Gabriel A. Elgavish</name><name sortKey="Hild, Gabor" sort="Hild, Gabor" uniqKey="Hild G" first="Gábor" last="Hild">Gábor Hild</name><name sortKey="Simor, Tamas" sort="Simor, Tamas" uniqKey="Simor T" first="Tamás" last="Simor">Tamás Simor</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Terre/explor/ThuliumV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002044 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002044 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Terre
|area= ThuliumV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:5B70B3D578596F3BC8D87F9255574C50DF49431D
|texte= The Effects of the NMR Shift-reagents Dy(PPP)2, Dy(TTHA) and Tm(DOTP) on Developed Pressure in Isolated Perfused Rat Hearts. The Role of Shift-reagent Calcium Complexes
}}
| This area was generated with Dilib version V0.6.21. |  |