Determination of liposomal encapsulation efficiency using proton NMR spectroscopy.
Identifieur interne : 001A40 ( Main/Exploration ); précédent : 001A39; suivant : 001A41Determination of liposomal encapsulation efficiency using proton NMR spectroscopy.
Auteurs : Xian-Man Zhang [États-Unis] ; Anant B. Patel ; Robin A. De Graaf ; Kevin L. BeharSource :
- Chemistry and physics of lipids [ 0009-3084 ] ; 2004.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , analogs & derivatives : Carnosine.
- chemical , chemistry : Carnosine, Deuterium, Liposomes, Organometallic Compounds, Organophosphorus Compounds, Phospholipids.
- Hydrogen-Ion Concentration, Magnetic Resonance Spectroscopy, Protons.
Abstract
A rapid and simple approach using 1H NMR was developed for determination of liposomal encapsulation efficiency without the need for physical separation of entrapped and non-entrapped marker. Measurements were made using a marker (homocarnosine) with a pH-sensitive 1H chemical shift in the presence of a pH gradient across the phospholipid vesicle membrane, or by addition of the chemical shift reagent, thulium(III)-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetra-(methylene phosphonic acid sodium salt) (TmDOTP5-). The measured encapsulation efficiencies for the liposomal dispersions prepared from 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) through extrusion using 50, 200 and 1000 nm polycarbonate membranes were found to be identical using the two different experimental approaches.
PubMed: 14706745
Affiliations:
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Le document en format XML
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De Graaf</name></author><author><name sortKey="Behar, Kevin L" sort="Behar, Kevin L" uniqKey="Behar K" first="Kevin L" last="Behar">Kevin L. Behar</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:14706745</idno><idno type="pmid">14706745</idno><idno type="wicri:Area/PubMed/Corpus">000684</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000684</idno><idno type="wicri:Area/PubMed/Curation">000684</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000684</idno><idno type="wicri:Area/PubMed/Checkpoint">000684</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000684</idno><idno type="wicri:Area/Ncbi/Merge">000092</idno><idno type="wicri:Area/Ncbi/Curation">000092</idno><idno type="wicri:Area/Ncbi/Checkpoint">000092</idno><idno type="wicri:doubleKey">0009-3084:2004:Zhang X:determination:of:liposomal</idno><idno type="wicri:Area/Main/Merge">001B04</idno><idno type="wicri:Area/Main/Curation">001A40</idno><idno type="wicri:Area/Main/Exploration">001A40</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Determination of liposomal encapsulation efficiency using proton NMR spectroscopy.</title><author><name sortKey="Zhang, Xian Man" sort="Zhang, Xian Man" uniqKey="Zhang X" first="Xian-Man" last="Zhang">Xian-Man Zhang</name><affiliation wicri:level="2"><nlm:affiliation>Department of Psychiatry, New Haven, CT 06520-8043, USA. xian-man.zhang@yale.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Psychiatry, New Haven, CT 06520-8043</wicri:regionArea><placeName><region type="state">Connecticut</region></placeName></affiliation></author><author><name sortKey="Patel, Anant B" sort="Patel, Anant B" uniqKey="Patel A" first="Anant B" last="Patel">Anant B. Patel</name></author><author><name sortKey="De Graaf, Robin A" sort="De Graaf, Robin A" uniqKey="De Graaf R" first="Robin A" last="De Graaf">Robin A. De Graaf</name></author><author><name sortKey="Behar, Kevin L" sort="Behar, Kevin L" uniqKey="Behar K" first="Kevin L" last="Behar">Kevin L. Behar</name></author></analytic><series><title level="j">Chemistry and physics of lipids</title><idno type="ISSN">0009-3084</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Carnosine (analogs & derivatives)</term><term>Carnosine (chemistry)</term><term>Deuterium (chemistry)</term><term>Hydrogen-Ion Concentration</term><term>Liposomes (chemistry)</term><term>Magnetic Resonance Spectroscopy</term><term>Organometallic Compounds (chemistry)</term><term>Organophosphorus Compounds (chemistry)</term><term>Phospholipids (chemistry)</term><term>Protons</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Carnosine ()</term><term>Carnosine (analogues et dérivés)</term><term>Composés organiques du phosphore ()</term><term>Composés organométalliques ()</term><term>Concentration en ions d'hydrogène</term><term>Deutérium ()</term><term>Liposomes ()</term><term>Phospholipides ()</term><term>Protons</term><term>Spectroscopie par résonance magnétique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Carnosine</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Carnosine</term><term>Deuterium</term><term>Liposomes</term><term>Organometallic Compounds</term><term>Organophosphorus Compounds</term><term>Phospholipids</term></keywords><keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Carnosine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Hydrogen-Ion Concentration</term><term>Magnetic Resonance Spectroscopy</term><term>Protons</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Carnosine</term><term>Composés organiques du phosphore</term><term>Composés organométalliques</term><term>Concentration en ions d'hydrogène</term><term>Deutérium</term><term>Liposomes</term><term>Phospholipides</term><term>Protons</term><term>Spectroscopie par résonance magnétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A rapid and simple approach using 1H NMR was developed for determination of liposomal encapsulation efficiency without the need for physical separation of entrapped and non-entrapped marker. Measurements were made using a marker (homocarnosine) with a pH-sensitive 1H chemical shift in the presence of a pH gradient across the phospholipid vesicle membrane, or by addition of the chemical shift reagent, thulium(III)-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetra-(methylene phosphonic acid sodium salt) (TmDOTP5-). The measured encapsulation efficiencies for the liposomal dispersions prepared from 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) through extrusion using 50, 200 and 1000 nm polycarbonate membranes were found to be identical using the two different experimental approaches.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Connecticut</li></region></list><tree><noCountry><name sortKey="Behar, Kevin L" sort="Behar, Kevin L" uniqKey="Behar K" first="Kevin L" last="Behar">Kevin L. Behar</name><name sortKey="De Graaf, Robin A" sort="De Graaf, Robin A" uniqKey="De Graaf R" first="Robin A" last="De Graaf">Robin A. De Graaf</name><name sortKey="Patel, Anant B" sort="Patel, Anant B" uniqKey="Patel A" first="Anant B" last="Patel">Anant B. Patel</name></noCountry><country name="États-Unis"><region name="Connecticut"><name sortKey="Zhang, Xian Man" sort="Zhang, Xian Man" uniqKey="Zhang X" first="Xian-Man" last="Zhang">Xian-Man Zhang</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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