ThuliumV1, Istex, Curation, bibRecord, 002368

Isolation and sequence analysis of TGA 1 cDNAs encoding a tomato G protein α subunit

Identifieur interne : 002368 ( Istex/Curation ); précédent : 002367; suivant : 002369

Isolation and sequence analysis of TGA 1 cDNAs encoding a tomato G protein α subunit

Auteurs : Ma Hong ; Martin F. Yanofsky ; Huang Hai

Source :

Gene [ 0378-1119 ] ; 1991.

RBID : ISTEX:A007C09A996CA1DEFEC1710F272AB3149828551C

English descriptors

KwdEn :
- Acad, Bourne, Cdna, Cdna clones, Cholera toxin, Church buffer, Clone, Codon, Cold spring harbor, Cold spring harbor laboratory, Consensus regions, Dgal, Encoding, Gene, Gpai, Gpal, Guanine proteins, Longest cdna, Natl, Pertussis toxin, Proc, Protein gene, Sequence information, Short palindromic sequences, Subunit, Tgai, Tgal, Tgal cdna, Thaliana, Thaliana gpai, Thaliana gpal gene, Tomato, Tomato genome, Tomato genomic, Tomato tgal, Yeast gcn4.
Teeft :
- Acad, Bourne, Cdna, Cdna clones, Cholera toxin, Church buffer, Clone, Codon, Cold spring harbor, Cold spring harbor laboratory, Consensus regions, Dgal, Encoding, Gene, Gpai, Gpal, Guanine proteins, Longest cdna, Natl, Pertussis toxin, Proc, Protein gene, Sequence information, Short palindromic sequences, Subunit, Tgai, Tgal, Tgal cdna, Thaliana, Thaliana gpai, Thaliana gpal gene, Tomato, Tomato genome, Tomato genomic, Tomato tgal, Yeast gcn4.

Abstract

Abstract: We have isolated cDNAs for a gene coding for a G protein a subunit from tomato (Lycopersicon esculentum, cv. VF36). This gene, named TGA1, was isolated using a cDNA of the Arabidopsis thaliana G protein a subunit-encoding gene, GPA1, as a DNA probe. The sequences of four cDNA clones indicate that the deduced amino acid (aa) sequence of the gene product (TGal) has 384 aa (44906 Da). The predicted TGαl protein exhibits similarity to all known G protein α subunits. The aa are 84.6% identical and 93% similar (identical and conservative changes) to A. thaliana GPαl, and 34% identical and 59% similar to mammalian transducins. Furthermore, it has all of the consensus regions for a GTP-binding protein. Finally, hybridizations of tomato genomic DNA indicate that TGA1 is a single-copy gene.

Url:

https://api.istex.fr/document/A007C09A996CA1DEFEC1710F272AB3149828551C/fulltext/pdf

DOI: 10.1016/0378-1119(91)90318-6

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Ma Hong

<affiliation><mods:affiliation>Cold Spring H arbor Laboratory, Cold Spring Harbor, NY 11724-2212 U.S.A. Tel. (516)367-8330</mods:affiliation>
<wicri:noCountry code="subField">(516)367-8330</wicri:noCountry>
</affiliation>

Martin F. Yanofsky

<affiliation><mods:affiliation>Department of Biology, University of California at San Diego, La Jolla, CA 92093 U.S.A. Tel. (619)534-7299</mods:affiliation>
<wicri:noCountry code="subField">(619)534-7299</wicri:noCountry>
</affiliation>

Huang Hai

<affiliation><mods:affiliation>Cold Spring H arbor Laboratory, Cold Spring Harbor, NY 11724-2212 U.S.A. Tel. (516)367-8330</mods:affiliation>
<wicri:noCountry code="subField">(516)367-8330</wicri:noCountry>
</affiliation>

Le document en format XML

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<term>Tgal cdna</term>
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<front><div type="abstract" xml:lang="en">Abstract: We have isolated cDNAs for a gene coding for a G protein a subunit from tomato (Lycopersicon esculentum, cv. VF36). This gene, named TGA1, was isolated using a cDNA of the Arabidopsis thaliana G protein a subunit-encoding gene, GPA1, as a DNA probe. The sequences of four cDNA clones indicate that the deduced amino acid (aa) sequence of the gene product (TGal) has 384 aa (44906 Da). The predicted TGαl protein exhibits similarity to all known G protein α subunits. The aa are 84.6% identical and 93% similar (identical and conservative changes) to A. thaliana GPαl, and 34% identical and 59% similar to mammalian transducins. Furthermore, it has all of the consensus regions for a GTP-binding protein. Finally, hybridizations of tomato genomic DNA indicate that TGA1 is a single-copy gene.</div>
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Isolation and sequence analysis of TGA 1 cDNAs encoding a tomato G protein α subunit

Isolation and sequence analysis of TGA 1 cDNAs encoding a tomato G protein α subunit

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Curation

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Pour manipuler ce document sous Unix (Dilib)

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