UVB‐lnduced DNA Damage and Its Photorepair in Nuclei and Chloroplasts of Spinacia oleracea L.
Identifieur interne : 001486 ( Istex/Corpus ); précédent : 001485; suivant : 001487UVB‐lnduced DNA Damage and Its Photorepair in Nuclei and Chloroplasts of Spinacia oleracea L.
Auteurs : M. Hada ; T. Hashimoto ; O. Nikaido ; M. ShinSource :
- Photochemistry and Photobiology [ 0031-8655 ] ; 1998-09.
English descriptors
- KwdEn :
- Ammonium sulfate, Chloroplast, Cyclobutane pyrimidine dimer, Dark repair, Data show, Deionized water, Dimer, Elisa, Elisa system, Fresh weight equivalent, Hexadecyltrimethylammonium bromide, Immunosorbent assay, Kobe, Kobe university, Model riken, Monoclonal antibodies, Photolyase, Photolyase preparation, Photolyase system, Photoproducts, Photorepair, Photorepair system, Pyrimidine, Pyrimidine pyrimidinone photoproducts, Reaction mixture, Spectral photon distribution, Spinach, Spinacia oleracea, Subsequent exposure, Total volume, White light.
- Teeft :
- Ammonium sulfate, Chloroplast, Cyclobutane pyrimidine dimer, Dark repair, Data show, Deionized water, Dimer, Elisa, Elisa system, Fresh weight equivalent, Hexadecyltrimethylammonium bromide, Immunosorbent assay, Kobe, Kobe university, Model riken, Monoclonal antibodies, Photolyase, Photolyase preparation, Photolyase system, Photoproducts, Photorepair, Photorepair system, Pyrimidine, Pyrimidine pyrimidinone photoproducts, Reaction mixture, Spectral photon distribution, Spinach, Spinacia oleracea, Subsequent exposure, Total volume, White light.
Abstract
Ultraviolet‐B‐induced lesions and their photorepair in nuclear and chloroplast DNA of spinach (Spinacia oleracea L.) leaves were examined with two photoproducts, cyclobutane pyrimidine dimers (CPD) and pyrimidine (6‐4) pyrimidinone photoproducts (6‐4PP). These photoproducts were induced both in nuclear and chloroplast DNA by UVB irradiation and could be detected by enzyme‐linked immunosorbent assay using their respective monoclonal antibodies. Formation of CPD was greater in nuclear DNA than in chloroplast DNA (about 10 to 7), whereas 6‐4PP formation was comparable in both DNA. On subsequent exposure of leaves to blue/UVA after UVB irradiation, photorepair of CPD and 6‐4PP occurred in nuclear DNA but not in chloroplast DNA. When isolated chloroplasts were irradiated with UVB, CPD was also induced in their DNA. But photorepair of CPD did not occur in them by subsequent exposure to blue/UVA, suggesting that no photorepair system operates in chloroplasts.
Url:
DOI: 10.1111/j.1751-1097.1998.tb09688.x
Links to Exploration step
ISTEX:5E0FDC3E1DB5180DA787A11F68846463DEAE3ADFLe document en format XML
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Hada</name><affiliation><mods:affiliation>Department of Biology, Kobe University, Kobe, Japan</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: mhada@biol.kobe‐u.ac.jp</mods:affiliation></affiliation></author><author><name sortKey="Hashimoto, T" sort="Hashimoto, T" uniqKey="Hashimoto T" first="T." last="Hashimoto">T. Hashimoto</name><affiliation><mods:affiliation>Department of Life Science, Kobe Women's University, Kobe, Japan</mods:affiliation></affiliation></author><author><name sortKey="Nikaido, O" sort="Nikaido, O" uniqKey="Nikaido O" first="O." last="Nikaido">O. Nikaido</name><affiliation><mods:affiliation>Division of Radiation Biology, Kanazawa University, Kanazawa, Japan</mods:affiliation></affiliation></author><author><name sortKey="Shin, M" sort="Shin, M" uniqKey="Shin M" first="M." last="Shin">M. 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These photoproducts were induced both in nuclear and chloroplast DNA by UVB irradiation and could be detected by enzyme‐linked immunosorbent assay using their respective monoclonal antibodies. Formation of CPD was greater in nuclear DNA than in chloroplast DNA (about 10 to 7), whereas 6‐4PP formation was comparable in both DNA. On subsequent exposure of leaves to blue/UVA after UVB irradiation, photorepair of CPD and 6‐4PP occurred in nuclear DNA but not in chloroplast DNA. When isolated chloroplasts were irradiated with UVB, CPD was also induced in their DNA. 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On subsequent exposure of leaves to blue/UVA after UVB irradiation, photorepair of CPD and 6‐4PP occurred in nuclear DNA but not in chloroplast DNA. When isolated chloroplasts were irradiated with UVB, CPD was also induced in their DNA. 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Fax: 81‐78‐803‐0444; e‐mail: mhada@biol.kobe‐u.ac.jp</affiliation></author><author xml:id="author-0001"><persName><forename type="first">T.</forename><surname>Hashimoto</surname></persName><affiliation>Department of Life Science, Kobe Women's University, Kobe, Japan<address><country key="JP"></country></address></affiliation></author><author xml:id="author-0002"><persName><forename type="first">O.</forename><surname>Nikaido</surname></persName><affiliation>Division of Radiation Biology, Kanazawa University, Kanazawa, Japan<address><country key="JP"></country></address></affiliation></author><author xml:id="author-0003"><persName><forename type="first">M.</forename><surname>Shin</surname></persName><affiliation>Teru Biolaboratory, Mino‐shi, Osaka, Japan<address><country key="JP"></country></address></affiliation></author><idno type="istex">5E0FDC3E1DB5180DA787A11F68846463DEAE3ADF</idno><idno type="ark">ark:/67375/WNG-MWF99B15-B</idno><idno type="DOI">10.1111/j.1751-1097.1998.tb09688.x</idno><idno type="unit">PHP319</idno><idno type="toTypesetVersion">file:PHP.PHP319.pdf</idno></analytic><monogr><title level="j" type="main">Photochemistry and Photobiology</title><title level="j" type="alt">PHOTOCHEMISTRY PHOTOBIOLOGY</title><idno type="pISSN">0031-8655</idno><idno type="eISSN">1751-1097</idno><idno type="book-DOI">10.1111/(ISSN)1751-1097</idno><idno type="book-part-DOI">10.1111/php.1998.68.issue-3</idno><idno type="product">PHP</idno><idno type="publisherDivision">ST</idno><imprint><biblScope unit="vol">68</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="319">319</biblScope><biblScope unit="page" to="322">322</biblScope><biblScope unit="page-count">4</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1998-09"></date></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><abstract xml:lang="en" style="main"><head>ABSTRACT</head><p>Ultraviolet‐B‐induced lesions and their photorepair in nuclear and chloroplast DNA of spinach (<hi rend="italic">Spinacia oleracea</hi> L.) leaves were examined with two photoproducts, cyclobutane pyrimidine dimers (CPD) and pyrimidine (6‐4) pyrimidinone photoproducts (6‐4PP). These photoproducts were induced both in nuclear and chloroplast DNA by UVB irradiation and could be detected by enzyme‐linked immunosorbent assay using their respective monoclonal antibodies. Formation of CPD was greater in nuclear DNA than in chloroplast DNA (about 10 to 7), whereas 6‐4PP formation was comparable in both DNA. On subsequent exposure of leaves to blue/UVA after UVB irradiation, photorepair of CPD and 6‐4PP occurred in nuclear DNA but not in chloroplast DNA. When isolated chloroplasts were irradiated with UVB, CPD was also induced in their DNA. 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Fax: 81‐78‐803‐0444; e‐mail: <email normalForm="mhada@biol.kobe-u.ac.jp">mhada@biol.kobe‐u.ac.jp</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:PHP.PHP319.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>Received 18 February 1998; accepted 28 May 1998</unparsedEditorialHistory><countGroup><count type="referenceTotal" number="14"></count><count type="linksCrossRef" number="5"></count></countGroup><titleGroup><title type="main">UVB‐lnduced DNA Damage and Its Photorepair in Nuclei and Chloroplasts of Spinacia oleracea L.</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1" corresponding="yes"><personName><givenNames>M.</givenNames><familyName>Hada</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a2"><personName><givenNames>T.</givenNames><familyName>Hashimoto</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a3"><personName><givenNames>O.</givenNames><familyName>Nikaido</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a4"><personName><givenNames>M.</givenNames><familyName>Shin</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="JP"><unparsedAffiliation>Department of Biology, Kobe University, Kobe, Japan</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="JP"><unparsedAffiliation>Department of Life Science, Kobe Women's University, Kobe, Japan</unparsedAffiliation></affiliation><affiliation xml:id="a3" countryCode="JP"><unparsedAffiliation>Division of Radiation Biology, Kanazawa University, Kanazawa, Japan</unparsedAffiliation></affiliation><affiliation xml:id="a4" countryCode="JP"><unparsedAffiliation>Teru Biolaboratory, Mino‐shi, Osaka, Japan</unparsedAffiliation></affiliation></affiliationGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">ABSTRACT</title><p>Ultraviolet‐B‐induced lesions and their photorepair in nuclear and chloroplast DNA of spinach (<i>Spinacia oleracea</i> L.) leaves were examined with two photoproducts, cyclobutane pyrimidine dimers (CPD) and pyrimidine (6‐4) pyrimidinone photoproducts (6‐4PP). These photoproducts were induced both in nuclear and chloroplast DNA by UVB irradiation and could be detected by enzyme‐linked immunosorbent assay using their respective monoclonal antibodies. Formation of CPD was greater in nuclear DNA than in chloroplast DNA (about 10 to 7), whereas 6‐4PP formation was comparable in both DNA. On subsequent exposure of leaves to blue/UVA after UVB irradiation, photorepair of CPD and 6‐4PP occurred in nuclear DNA but not in chloroplast DNA. When isolated chloroplasts were irradiated with UVB, CPD was also induced in their DNA. But photorepair of CPD did not occur in them by subsequent exposure to blue/UVA, suggesting that no photorepair system operates in chloroplasts.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>UVB‐lnduced DNA Damage and Its Photorepair in Nuclei and Chloroplasts of Spinacia oleracea L.</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>UVB‐lnduced DNA Damage and Its Photorepair in Nuclei and Chloroplasts of Spinacia oleracea L.</title></titleInfo><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Hada</namePart><affiliation>Department of Biology, Kobe University, Kobe, Japan</affiliation><affiliation>E-mail: mhada@biol.kobe‐u.ac.jp</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">T.</namePart><namePart type="family">Hashimoto</namePart><affiliation>Department of Life Science, Kobe Women's University, Kobe, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">O.</namePart><namePart type="family">Nikaido</namePart><affiliation>Division of Radiation Biology, Kanazawa University, Kanazawa, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Shin</namePart><affiliation>Teru Biolaboratory, Mino‐shi, Osaka, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">1998-09</dateIssued><edition>Received 18 February 1998; accepted 28 May 1998</edition><copyrightDate encoding="w3cdtf">1998</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><extent unit="references">14</extent><extent unit="linksCrossRef">5</extent></physicalDescription><abstract lang="en">Ultraviolet‐B‐induced lesions and their photorepair in nuclear and chloroplast DNA of spinach (Spinacia oleracea L.) leaves were examined with two photoproducts, cyclobutane pyrimidine dimers (CPD) and pyrimidine (6‐4) pyrimidinone photoproducts (6‐4PP). These photoproducts were induced both in nuclear and chloroplast DNA by UVB irradiation and could be detected by enzyme‐linked immunosorbent assay using their respective monoclonal antibodies. Formation of CPD was greater in nuclear DNA than in chloroplast DNA (about 10 to 7), whereas 6‐4PP formation was comparable in both DNA. On subsequent exposure of leaves to blue/UVA after UVB irradiation, photorepair of CPD and 6‐4PP occurred in nuclear DNA but not in chloroplast DNA. When isolated chloroplasts were irradiated with UVB, CPD was also induced in their DNA. But photorepair of CPD did not occur in them by subsequent exposure to blue/UVA, suggesting that no photorepair system operates in chloroplasts.</abstract><relatedItem type="host"><titleInfo><title>Photochemistry and Photobiology</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><identifier type="ISSN">0031-8655</identifier><identifier type="eISSN">1751-1097</identifier><identifier type="DOI">10.1111/(ISSN)1751-1097</identifier><identifier type="PublisherID">PHP</identifier><part><date>1998</date><detail type="volume"><caption>vol.</caption><number>68</number></detail><detail type="issue"><caption>no.</caption><number>3</number></detail><extent unit="pages"><start>319</start><end>322</end><total>4</total></extent></part></relatedItem><identifier type="istex">5E0FDC3E1DB5180DA787A11F68846463DEAE3ADF</identifier><identifier type="ark">ark:/67375/WNG-MWF99B15-B</identifier><identifier type="DOI">10.1111/j.1751-1097.1998.tb09688.x</identifier><identifier type="ArticleID">PHP319</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-L0C46X92-X">wiley</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/document/5E0FDC3E1DB5180DA787A11F68846463DEAE3ADF/metadata/json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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