Inexpensive Method for measuring Tritium in the Presence of Caesium Chloride
Identifieur interne : 002B01 ( Istex/Checkpoint ); précédent : 002B00; suivant : 002B02Inexpensive Method for measuring Tritium in the Presence of Caesium Chloride
Auteurs : A. P. PhillipsSource :
- Nature [ 0028-0836 ] ; 1969-09-06.
Abstract
IT is difficult to assay a weak -emitter such as tritium in the presence of high salt concentrations, for example, after the equilibrium sedimentation of radioactively labelled nucleic acids in density gradients of caesium chloride. The insolubility of DNA in dilute perchloric or trichloroacetic acids allows DNA trapped in filter paper to be washed free of CsCl. Such a convenient precipitation method, however, is not always applicable. A technique has therefore been developed in this laboratory for the assay of tritium in the presence of CsCl. The method allows a counting efficiency comparable with that obtained with a commercial dioxan-based scintillator, but at a fraction of the cost.
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DOI: 10.1038/2231055b0
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