Observation of intracellular potassium and sodium in the heart by NMR: A major fraction of potassium is “invisible”
Identifieur interne : 001C16 ( Istex/Checkpoint ); précédent : 001C15; suivant : 001C17Observation of intracellular potassium and sodium in the heart by NMR: A major fraction of potassium is “invisible”
Auteurs : Eric T. Fossel [États-Unis] ; Herbert Hoefeler [États-Unis]Source :
- Magnetic Resonance in Medicine [ 0740-3194 ] ; 1986-08.
Descripteurs français
- Wicri :
- topic : Potassium.
English descriptors
- KwdEn :
- Academic press, Activity measurements, American heart association, Arbitrary area units, Calibration spectra, Cell water, Cellular water, Experimental conditions, Extracellular, Flame photometry, Fossel, Interstitial spaces, Intracellular, Intracellular concentrations, Intracellular potassium, Intracellular sodium, Intracellular sodium concentration, Latex balloon, Linewidth, Magnetic resonance, Major fraction, Microelectrodes, Perfusate, Perfused, Perfused heart, Potassium, Reagent, Shift reagent, Shift reagents, Visible fraction, Visible intracellular potassium, Visible sodium.
- Teeft :
- Academic press, Activity measurements, American heart association, Arbitrary area units, Calibration spectra, Cell water, Cellular water, Experimental conditions, Extracellular, Flame photometry, Fossel, Interstitial spaces, Intracellular, Intracellular concentrations, Intracellular potassium, Intracellular sodium, Intracellular sodium concentration, Latex balloon, Linewidth, Magnetic resonance, Major fraction, Microelectrodes, Perfusate, Perfused, Perfused heart, Potassium, Reagent, Shift reagent, Shift reagents, Visible fraction, Visible intracellular potassium, Visible sodium.
Abstract
Using 39K and 23Na NMR in conjunetion with extracellularly localized shift reagents, we have determined the intracellular concentrations of NMR visible sodium and potassium in isolated, perfused rat hearts. We find this concentration to be 9.9 mM/kg cell water for sodium and 31 mM/kg cell water for potassium. Values of activity determined by ionsensitive microelectrodes are in good agreement with our sodium value but do not agree with our potassium value. Our results mean that a major pool of intracelluar potassium is, on average, signifieantly immobilized and that the mobile NMR visible faction (31mM/kg) is not in exchange with the NMR invisible pool (114mM/kg). The immobilized fraction is characterized by T2 values which are too short to be observed by our conventional spectrometer. This fraction is, therefore, said to be “invisible” under our experimental conditions. © 1986 Academic Press, Inc.
Url:
DOI: 10.1002/mrm.1910030406
Affiliations:
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Fossel</name></author><author><name sortKey="Hoefeler, Herbert" sort="Hoefeler, Herbert" uniqKey="Hoefeler H" first="Herbert" last="Hoefeler">Herbert Hoefeler</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:5C2DC64AE57F5919567E524DF7C2E7A14392B015</idno><date when="1986" year="1986">1986</date><idno type="doi">10.1002/mrm.1910030406</idno><idno type="url">https://api.istex.fr/document/5C2DC64AE57F5919567E524DF7C2E7A14392B015/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001442</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001442</idno><idno type="wicri:Area/Istex/Curation">001442</idno><idno type="wicri:Area/Istex/Checkpoint">001C16</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001C16</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Observation of intracellular potassium and sodium in the heart by NMR: A major fraction of potassium is “invisible”</title><author><name sortKey="Fossel, Eric T" sort="Fossel, Eric T" uniqKey="Fossel E" first="Eric T." last="Fossel">Eric T. 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Dana Research Instiute, Radiology Research Center, Beth Israel Hospital and Harvard Medical School, Boston</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Magnetic Resonance in Medicine</title><title level="j" type="alt">MAGNETIC RESONANCE IN MEDICINE</title><idno type="ISSN">0740-3194</idno><idno type="eISSN">1522-2594</idno><imprint><biblScope unit="vol">3</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="534">534</biblScope><biblScope unit="page" to="540">540</biblScope><biblScope unit="page-count">7</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Baltimore</pubPlace><date type="published" when="1986-08">1986-08</date></imprint><idno type="ISSN">0740-3194</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0740-3194</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Academic press</term><term>Activity measurements</term><term>American heart association</term><term>Arbitrary area units</term><term>Calibration spectra</term><term>Cell water</term><term>Cellular water</term><term>Experimental conditions</term><term>Extracellular</term><term>Flame photometry</term><term>Fossel</term><term>Interstitial spaces</term><term>Intracellular</term><term>Intracellular concentrations</term><term>Intracellular potassium</term><term>Intracellular sodium</term><term>Intracellular sodium concentration</term><term>Latex balloon</term><term>Linewidth</term><term>Magnetic resonance</term><term>Major fraction</term><term>Microelectrodes</term><term>Perfusate</term><term>Perfused</term><term>Perfused heart</term><term>Potassium</term><term>Reagent</term><term>Shift reagent</term><term>Shift reagents</term><term>Visible fraction</term><term>Visible intracellular potassium</term><term>Visible sodium</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Academic press</term><term>Activity measurements</term><term>American heart association</term><term>Arbitrary area units</term><term>Calibration spectra</term><term>Cell water</term><term>Cellular water</term><term>Experimental conditions</term><term>Extracellular</term><term>Flame photometry</term><term>Fossel</term><term>Interstitial spaces</term><term>Intracellular</term><term>Intracellular concentrations</term><term>Intracellular potassium</term><term>Intracellular sodium</term><term>Intracellular sodium concentration</term><term>Latex balloon</term><term>Linewidth</term><term>Magnetic resonance</term><term>Major fraction</term><term>Microelectrodes</term><term>Perfusate</term><term>Perfused</term><term>Perfused heart</term><term>Potassium</term><term>Reagent</term><term>Shift reagent</term><term>Shift reagents</term><term>Visible fraction</term><term>Visible intracellular potassium</term><term>Visible sodium</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Potassium</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Using 39K and 23Na NMR in conjunetion with extracellularly localized shift reagents, we have determined the intracellular concentrations of NMR visible sodium and potassium in isolated, perfused rat hearts. We find this concentration to be 9.9 mM/kg cell water for sodium and 31 mM/kg cell water for potassium. Values of activity determined by ionsensitive microelectrodes are in good agreement with our sodium value but do not agree with our potassium value. Our results mean that a major pool of intracelluar potassium is, on average, signifieantly immobilized and that the mobile NMR visible faction (31mM/kg) is not in exchange with the NMR invisible pool (114mM/kg). The immobilized fraction is characterized by T2 values which are too short to be observed by our conventional spectrometer. This fraction is, therefore, said to be “invisible” under our experimental conditions. © 1986 Academic Press, Inc.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Massachusetts</li></region></list><tree><country name="États-Unis"><region name="Massachusetts"><name sortKey="Fossel, Eric T" sort="Fossel, Eric T" uniqKey="Fossel E" first="Eric T." last="Fossel">Eric T. Fossel</name></region><name sortKey="Hoefeler, Herbert" sort="Hoefeler, Herbert" uniqKey="Hoefeler H" first="Herbert" last="Hoefeler">Herbert Hoefeler</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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