Synthesis of biosurfactant stabilized silver nanoparticles, characterization and their potential application for bactericidal purposes.
Identifieur interne : 000151 ( Main/Curation ); précédent : 000150; suivant : 000152Synthesis of biosurfactant stabilized silver nanoparticles, characterization and their potential application for bactericidal purposes.
Auteurs : Fisseha A. Bezza [Afrique du Sud] ; Shepherd M. Tichapondwa [Afrique du Sud] ; Evans M N. Chirwa [Afrique du Sud]Source :
- Journal of hazardous materials [ 1873-3336 ] ; 2020.
Abstract
Uniformly dispersed silver nanoparticles (AgNPs) with remarkable colloidal stability were synthesised using chemical reduction method in lipopeptide biosurfactant reverse micelles. Transmission Electron microscopy (TEM), Scanning electron microscopy (SEM) and UV-vis spectroscopy analysis exhibited monodisperse nanoparticles with spherical morphology of diameter of 21 ± 2. The lipopeptide stabilized AgNPs displayed remarkable antibacterial activity with minimum inhibitory concentration (MIC) value of 15.625 μg/mL against Gram-negative Pseudomonas aeruginosa CB1 and Gram-positive Bacillus subtilis CN2 strains with a significant dose-dependent reduction of cell viability and loss of membrane integrity. Investigation of AgNPs internalization and dissolution assays demonstrated 42-fold higher leaching of the lipopeptide-stabilized AgNPs compared to the bare AgNPs, and concentration dependent increase in cellular uptake with subsequent damage to intracellular organelles. Further ultrastructural observation using TEM revealed internalization and strong binding of considerable amount of AgNPs on the lipopolysaccharide layer of the Gram-negative and peptidoglycans layer of Gram-positive bacteria indiscriminately, demonstrating robust antibacterial activity and potential application to treat multidrug resistant bacteria.
DOI: 10.1016/j.jhazmat.2020.122319
PubMed: 32120206
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Tichapondwa</name><affiliation wicri:level="1"><nlm:affiliation>Water Utilization and Environmental Engineering Division, Department of Chemical Engineering, University of Pretoria, Pretoria 0002, South Africa.</nlm:affiliation><country xml:lang="fr">Afrique du Sud</country><wicri:regionArea>Water Utilization and Environmental Engineering Division, Department of Chemical Engineering, University of Pretoria, Pretoria 0002</wicri:regionArea></affiliation></author><author><name sortKey="Chirwa, Evans M N" sort="Chirwa, Evans M N" uniqKey="Chirwa E" first="Evans M N" last="Chirwa">Evans M N. Chirwa</name><affiliation wicri:level="1"><nlm:affiliation>Water Utilization and Environmental Engineering Division, Department of Chemical Engineering, University of Pretoria, Pretoria 0002, South Africa. Electronic address: evans.chirwa@up.ac.za.</nlm:affiliation><country xml:lang="fr">Afrique du Sud</country><wicri:regionArea>Water Utilization and Environmental Engineering Division, Department of Chemical Engineering, University of Pretoria, Pretoria 0002</wicri:regionArea></affiliation></author></analytic><series><title level="j">Journal of hazardous materials</title><idno type="eISSN">1873-3336</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Uniformly dispersed silver nanoparticles (AgNPs) with remarkable colloidal stability were synthesised using chemical reduction method in lipopeptide biosurfactant reverse micelles. Transmission Electron microscopy (TEM), Scanning electron microscopy (SEM) and UV-vis spectroscopy analysis exhibited monodisperse nanoparticles with spherical morphology of diameter of 21 ± 2. The lipopeptide stabilized AgNPs displayed remarkable antibacterial activity with minimum inhibitory concentration (MIC) value of 15.625 μg/mL against Gram-negative Pseudomonas aeruginosa CB1 and Gram-positive Bacillus subtilis CN2 strains with a significant dose-dependent reduction of cell viability and loss of membrane integrity. Investigation of AgNPs internalization and dissolution assays demonstrated 42-fold higher leaching of the lipopeptide-stabilized AgNPs compared to the bare AgNPs, and concentration dependent increase in cellular uptake with subsequent damage to intracellular organelles. Further ultrastructural observation using TEM revealed internalization and strong binding of considerable amount of AgNPs on the lipopolysaccharide layer of the Gram-negative and peptidoglycans layer of Gram-positive bacteria indiscriminately, demonstrating robust antibacterial activity and potential application to treat multidrug resistant bacteria.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32120206</PMID><DateRevised><Year>2020</Year><Month>06</Month><Day>18</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-3336</ISSN><JournalIssue CitedMedium="Internet"><Volume>393</Volume><PubDate><Year>2020</Year><Month>07</Month><Day>05</Day></PubDate></JournalIssue><Title>Journal of hazardous materials</Title><ISOAbbreviation>J Hazard Mater</ISOAbbreviation></Journal><ArticleTitle>Synthesis of biosurfactant stabilized silver nanoparticles, characterization and their potential application for bactericidal purposes.</ArticleTitle><Pagination><MedlinePgn>122319</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0304-3894(20)30307-1</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jhazmat.2020.122319</ELocationID><Abstract><AbstractText>Uniformly dispersed silver nanoparticles (AgNPs) with remarkable colloidal stability were synthesised using chemical reduction method in lipopeptide biosurfactant reverse micelles. Transmission Electron microscopy (TEM), Scanning electron microscopy (SEM) and UV-vis spectroscopy analysis exhibited monodisperse nanoparticles with spherical morphology of diameter of 21 ± 2. The lipopeptide stabilized AgNPs displayed remarkable antibacterial activity with minimum inhibitory concentration (MIC) value of 15.625 μg/mL against Gram-negative Pseudomonas aeruginosa CB1 and Gram-positive Bacillus subtilis CN2 strains with a significant dose-dependent reduction of cell viability and loss of membrane integrity. Investigation of AgNPs internalization and dissolution assays demonstrated 42-fold higher leaching of the lipopeptide-stabilized AgNPs compared to the bare AgNPs, and concentration dependent increase in cellular uptake with subsequent damage to intracellular organelles. Further ultrastructural observation using TEM revealed internalization and strong binding of considerable amount of AgNPs on the lipopolysaccharide layer of the Gram-negative and peptidoglycans layer of Gram-positive bacteria indiscriminately, demonstrating robust antibacterial activity and potential application to treat multidrug resistant bacteria.</AbstractText><CopyrightInformation>Copyright © 2020 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bezza</LastName><ForeName>Fisseha A</ForeName><Initials>FA</Initials><AffiliationInfo><Affiliation>Water Utilization and Environmental Engineering Division, Department of Chemical Engineering, University of Pretoria, Pretoria 0002, South Africa.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tichapondwa</LastName><ForeName>Shepherd M</ForeName><Initials>SM</Initials><AffiliationInfo><Affiliation>Water Utilization and Environmental Engineering Division, Department of Chemical Engineering, University of Pretoria, Pretoria 0002, South Africa.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chirwa</LastName><ForeName>Evans M N</ForeName><Initials>EMN</Initials><AffiliationInfo><Affiliation>Water Utilization and Environmental Engineering Division, Department of Chemical Engineering, University of Pretoria, Pretoria 0002, South Africa. 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