The influence of growth medium on serum sensitivity of Bacteroides species.
Identifieur interne : 000C93 ( Main/Corpus ); précédent : 000C92; suivant : 000C94The influence of growth medium on serum sensitivity of Bacteroides species.
Auteurs : E. Allan ; I R PoxtonSource :
- Journal of medical microbiology [ 0022-2615 ] ; 1994.
English descriptors
- KwdEn :
- Bacterial Capsules (isolation & purification), Bacteroides (growth & development), Bacteroides (immunology), Blood Bactericidal Activity (MeSH), Centrifugation, Density Gradient (MeSH), Complement System Proteins (immunology), Culture Media (MeSH), Electrophoresis, Polyacrylamide Gel (MeSH), Humans (MeSH), Lipopolysaccharides (analysis), Silver Staining (MeSH), Specimen Handling (MeSH).
- MESH :
- chemical , analysis : Lipopolysaccharides.
- chemical , immunology : Complement System Proteins.
- growth & development : Bacteroides.
- immunology : Bacteroides.
- isolation & purification : Bacterial Capsules.
- Blood Bactericidal Activity, Centrifugation, Density Gradient, Culture Media, Electrophoresis, Polyacrylamide Gel, Humans, Silver Staining, Specimen Handling.
Abstract
The susceptibility of 12 different Bacteroides strains (representing nine species) to the bactericidal effect of human serum complement was investigated. When grown in nutrient-rich proteose peptone-yeast extract medium, all 12 strains were, to varying degrees, sensitive to serum. However, when grown in Van Tassell and Wilkins's minimal medium, six of the 12 strains became markedly more serum resistant. Five of these six strains became totally resistant to serum when grown in heat-inactivated (56 degrees C, 30 min) sheep serum. By Percoll discontinuous density centrifugation and light microscopy, the ratio of bacteria with large and small capsules was found to vary with the growth medium used. Lipopolysaccharide (LPS) was extracted with aqueous phenol after growth in the three media. Polyacrylamide gel electrophoresis (PAGE) and silver staining of the LPS showed some differences in LPS profiles in all strains tested. Therefore, variation of growth conditions results in alterations of both the expression of surface structures and, in some cases, sensitivity to serum. The biochemical basis for these changes requires further investigation.
DOI: 10.1099/00222615-41-1-45
PubMed: 8006944
Links to Exploration step
pubmed:8006944Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The influence of growth medium on serum sensitivity of Bacteroides species.</title><author><name sortKey="Allan, E" sort="Allan, E" uniqKey="Allan E" first="E" last="Allan">E. Allan</name><affiliation><nlm:affiliation>Department of Medical Microbiology, University of Edinburgh Medical School.</nlm:affiliation></affiliation></author><author><name sortKey="Poxton, I R" sort="Poxton, I R" uniqKey="Poxton I" first="I R" last="Poxton">I R Poxton</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1994">1994</date><idno type="RBID">pubmed:8006944</idno><idno type="pmid">8006944</idno><idno type="doi">10.1099/00222615-41-1-45</idno><idno type="wicri:Area/Main/Corpus">000C93</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000C93</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The influence of growth medium on serum sensitivity of Bacteroides species.</title><author><name sortKey="Allan, E" sort="Allan, E" uniqKey="Allan E" first="E" last="Allan">E. Allan</name><affiliation><nlm:affiliation>Department of Medical Microbiology, University of Edinburgh Medical School.</nlm:affiliation></affiliation></author><author><name sortKey="Poxton, I R" sort="Poxton, I R" uniqKey="Poxton I" first="I R" last="Poxton">I R Poxton</name></author></analytic><series><title level="j">Journal of medical microbiology</title><idno type="ISSN">0022-2615</idno><imprint><date when="1994" type="published">1994</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacterial Capsules (isolation & purification)</term><term>Bacteroides (growth & development)</term><term>Bacteroides (immunology)</term><term>Blood Bactericidal Activity (MeSH)</term><term>Centrifugation, Density Gradient (MeSH)</term><term>Complement System Proteins (immunology)</term><term>Culture Media (MeSH)</term><term>Electrophoresis, Polyacrylamide Gel (MeSH)</term><term>Humans (MeSH)</term><term>Lipopolysaccharides (analysis)</term><term>Silver Staining (MeSH)</term><term>Specimen Handling (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Lipopolysaccharides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Complement System Proteins</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Bacteroides</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Bacteroides</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Bacterial Capsules</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Blood Bactericidal Activity</term><term>Centrifugation, Density Gradient</term><term>Culture Media</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Humans</term><term>Silver Staining</term><term>Specimen Handling</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The susceptibility of 12 different Bacteroides strains (representing nine species) to the bactericidal effect of human serum complement was investigated. When grown in nutrient-rich proteose peptone-yeast extract medium, all 12 strains were, to varying degrees, sensitive to serum. However, when grown in Van Tassell and Wilkins's minimal medium, six of the 12 strains became markedly more serum resistant. Five of these six strains became totally resistant to serum when grown in heat-inactivated (56 degrees C, 30 min) sheep serum. By Percoll discontinuous density centrifugation and light microscopy, the ratio of bacteria with large and small capsules was found to vary with the growth medium used. Lipopolysaccharide (LPS) was extracted with aqueous phenol after growth in the three media. Polyacrylamide gel electrophoresis (PAGE) and silver staining of the LPS showed some differences in LPS profiles in all strains tested. Therefore, variation of growth conditions results in alterations of both the expression of surface structures and, in some cases, sensitivity to serum. The biochemical basis for these changes requires further investigation.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">8006944</PMID><DateCompleted><Year>1994</Year><Month>07</Month><Day>21</Day></DateCompleted><DateRevised><Year>2012</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0022-2615</ISSN><JournalIssue CitedMedium="Print"><Volume>41</Volume><Issue>1</Issue><PubDate><Year>1994</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Journal of medical microbiology</Title><ISOAbbreviation>J Med Microbiol</ISOAbbreviation></Journal><ArticleTitle>The influence of growth medium on serum sensitivity of Bacteroides species.</ArticleTitle><Pagination><MedlinePgn>45-50</MedlinePgn></Pagination><Abstract><AbstractText>The susceptibility of 12 different Bacteroides strains (representing nine species) to the bactericidal effect of human serum complement was investigated. When grown in nutrient-rich proteose peptone-yeast extract medium, all 12 strains were, to varying degrees, sensitive to serum. However, when grown in Van Tassell and Wilkins's minimal medium, six of the 12 strains became markedly more serum resistant. Five of these six strains became totally resistant to serum when grown in heat-inactivated (56 degrees C, 30 min) sheep serum. By Percoll discontinuous density centrifugation and light microscopy, the ratio of bacteria with large and small capsules was found to vary with the growth medium used. Lipopolysaccharide (LPS) was extracted with aqueous phenol after growth in the three media. Polyacrylamide gel electrophoresis (PAGE) and silver staining of the LPS showed some differences in LPS profiles in all strains tested. Therefore, variation of growth conditions results in alterations of both the expression of surface structures and, in some cases, sensitivity to serum. The biochemical basis for these changes requires further investigation.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Allan</LastName><ForeName>E</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Department of Medical Microbiology, University of Edinburgh Medical School.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Poxton</LastName><ForeName>I R</ForeName><Initials>IR</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Med Microbiol</MedlineTA><NlmUniqueID>0224131</NlmUniqueID><ISSNLinking>0022-2615</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003470">Culture Media</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008070">Lipopolysaccharides</NameOfSubstance></Chemical><Chemical><RegistryNumber>9007-36-7</RegistryNumber><NameOfSubstance UI="D003165">Complement System Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D016667" MajorTopicYN="N">Bacterial Capsules</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001439" MajorTopicYN="N">Bacteroides</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001770" MajorTopicYN="Y">Blood Bactericidal Activity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002499" MajorTopicYN="N">Centrifugation, Density Gradient</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003165" MajorTopicYN="N">Complement System Proteins</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003470" MajorTopicYN="N">Culture Media</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004591" MajorTopicYN="N">Electrophoresis, Polyacrylamide Gel</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008070" MajorTopicYN="N">Lipopolysaccharides</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016622" MajorTopicYN="N">Silver Staining</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013048" MajorTopicYN="N">Specimen Handling</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1994</Year><Month>7</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1994</Year><Month>7</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1994</Year><Month>7</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">8006944</ArticleId><ArticleId IdType="doi">10.1099/00222615-41-1-45</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Terre/explor/SilverBacteriV1/Data/Main/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000C93 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 000C93 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Terre
|area= SilverBacteriV1
|flux= Main
|étape= Corpus
|type= RBID
|clé= pubmed:8006944
|texte= The influence of growth medium on serum sensitivity of Bacteroides species.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Corpus/RBID.i -Sk "pubmed:8006944" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a SilverBacteriV1
| This area was generated with Dilib version V0.6.38. |  |