Synergistic effects between silver nanoparticles and antibiotics and the mechanisms involved.
Identifieur interne : 000983 ( Main/Corpus ); précédent : 000982; suivant : 000984Synergistic effects between silver nanoparticles and antibiotics and the mechanisms involved.
Auteurs : In-Sok Hwang ; Ji Hong Hwang ; Hyemin Choi ; Keuk-Jun Kim ; Dong Gun LeeSource :
- Journal of medical microbiology [ 1473-5644 ] ; 2012.
English descriptors
- KwdEn :
- Anti-Bacterial Agents (pharmacology), Bacteria (drug effects), Drug Synergism (MeSH), Enterococcus faecium (drug effects), Escherichia coli (drug effects), Metal Nanoparticles (MeSH), Microbial Sensitivity Tests (MeSH), Pseudomonas aeruginosa (drug effects), Silver (pharmacology), Staphylococcus aureus (drug effects), Streptococcus mutans (drug effects).
- MESH :
- chemical , pharmacology : Anti-Bacterial Agents, Silver.
- drug effects : Bacteria, Enterococcus faecium, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus mutans.
- Drug Synergism, Metal Nanoparticles, Microbial Sensitivity Tests.
Abstract
Silver nanoparticles (nano-Ags), which have well-known antimicrobial properties, are used extensively in various medical and general applications. In this study, the combination effects between nano-Ags and the conventional antibiotics ampicillin, chloramphenicol and kanamycin against various pathogenic bacteria were investigated. The MIC and fractional inhibitory concentration index (FICI) were determined to confirm antibacterial susceptibility and synergistic effects. The results showed that nano-Ags possessed antibacterial effects and synergistic activities. The antibiofilm activities of nano-Ags alone or in combination with antibiotics were also investigated. Formation of biofilm is associated with resistance to antimicrobial agents and chronic bacterial infections. The results indicated that nano-Ags also had antibiofilm activities. To understand these effects of nano-Ags, an ATPase inhibitor assay, permeability assay and hydroxyl radical assay were conducted. The antibacterial activity of nano-Ags was influenced by ATP-associated metabolism rather than by the permeability of the outer membrane. Additionally, nano-Ags generated hydroxyl radicals, a highly reactive oxygen species induced by bactericidal agents. It was concluded that nano-Ags have potential as a combination therapeutic agent for the treatment of infectious diseases by bacteria.
DOI: 10.1099/jmm.0.047100-0
PubMed: 22956753
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pubmed:22956753Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Synergistic effects between silver nanoparticles and antibiotics and the mechanisms involved.</title><author><name sortKey="Hwang, In Sok" sort="Hwang, In Sok" uniqKey="Hwang I" first="In-Sok" last="Hwang">In-Sok Hwang</name><affiliation><nlm:affiliation>School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Daehak-ro 80 Buk-gu Daegu, 702-701, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Hwang, Ji Hong" sort="Hwang, Ji Hong" uniqKey="Hwang J" first="Ji Hong" last="Hwang">Ji Hong Hwang</name><affiliation><nlm:affiliation>School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Daehak-ro 80 Buk-gu Daegu, 702-701, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Choi, Hyemin" sort="Choi, Hyemin" uniqKey="Choi H" first="Hyemin" last="Choi">Hyemin Choi</name><affiliation><nlm:affiliation>School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Daehak-ro 80 Buk-gu Daegu, 702-701, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Kim, Keuk Jun" sort="Kim, Keuk Jun" uniqKey="Kim K" first="Keuk-Jun" last="Kim">Keuk-Jun Kim</name><affiliation><nlm:affiliation>Department of Clinical Pathology, Tae Kyeung College, 24, Danbuk-ri, Jain-myeon, Gyeongsan-si, Gyeongsangbuk-do, 712-719, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Lee, Dong Gun" sort="Lee, Dong Gun" uniqKey="Lee D" first="Dong Gun" last="Lee">Dong Gun Lee</name><affiliation><nlm:affiliation>School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Daehak-ro 80 Buk-gu Daegu, 702-701, Republic of Korea.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22956753</idno><idno type="pmid">22956753</idno><idno type="doi">10.1099/jmm.0.047100-0</idno><idno type="wicri:Area/Main/Corpus">000983</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000983</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Synergistic effects between silver nanoparticles and antibiotics and the mechanisms involved.</title><author><name sortKey="Hwang, In Sok" sort="Hwang, In Sok" uniqKey="Hwang I" first="In-Sok" last="Hwang">In-Sok Hwang</name><affiliation><nlm:affiliation>School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Daehak-ro 80 Buk-gu Daegu, 702-701, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Hwang, Ji Hong" sort="Hwang, Ji Hong" uniqKey="Hwang J" first="Ji Hong" last="Hwang">Ji Hong Hwang</name><affiliation><nlm:affiliation>School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Daehak-ro 80 Buk-gu Daegu, 702-701, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Choi, Hyemin" sort="Choi, Hyemin" uniqKey="Choi H" first="Hyemin" last="Choi">Hyemin Choi</name><affiliation><nlm:affiliation>School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Daehak-ro 80 Buk-gu Daegu, 702-701, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Kim, Keuk Jun" sort="Kim, Keuk Jun" uniqKey="Kim K" first="Keuk-Jun" last="Kim">Keuk-Jun Kim</name><affiliation><nlm:affiliation>Department of Clinical Pathology, Tae Kyeung College, 24, Danbuk-ri, Jain-myeon, Gyeongsan-si, Gyeongsangbuk-do, 712-719, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Lee, Dong Gun" sort="Lee, Dong Gun" uniqKey="Lee D" first="Dong Gun" last="Lee">Dong Gun Lee</name><affiliation><nlm:affiliation>School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Daehak-ro 80 Buk-gu Daegu, 702-701, Republic of Korea.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Journal of medical microbiology</title><idno type="eISSN">1473-5644</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anti-Bacterial Agents (pharmacology)</term><term>Bacteria (drug effects)</term><term>Drug Synergism (MeSH)</term><term>Enterococcus faecium (drug effects)</term><term>Escherichia coli (drug effects)</term><term>Metal Nanoparticles (MeSH)</term><term>Microbial Sensitivity Tests (MeSH)</term><term>Pseudomonas aeruginosa (drug effects)</term><term>Silver (pharmacology)</term><term>Staphylococcus aureus (drug effects)</term><term>Streptococcus mutans (drug effects)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Anti-Bacterial Agents</term><term>Silver</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Bacteria</term><term>Enterococcus faecium</term><term>Escherichia coli</term><term>Pseudomonas aeruginosa</term><term>Staphylococcus aureus</term><term>Streptococcus mutans</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Drug Synergism</term><term>Metal Nanoparticles</term><term>Microbial Sensitivity Tests</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Silver nanoparticles (nano-Ags), which have well-known antimicrobial properties, are used extensively in various medical and general applications. In this study, the combination effects between nano-Ags and the conventional antibiotics ampicillin, chloramphenicol and kanamycin against various pathogenic bacteria were investigated. The MIC and fractional inhibitory concentration index (FICI) were determined to confirm antibacterial susceptibility and synergistic effects. The results showed that nano-Ags possessed antibacterial effects and synergistic activities. The antibiofilm activities of nano-Ags alone or in combination with antibiotics were also investigated. Formation of biofilm is associated with resistance to antimicrobial agents and chronic bacterial infections. The results indicated that nano-Ags also had antibiofilm activities. To understand these effects of nano-Ags, an ATPase inhibitor assay, permeability assay and hydroxyl radical assay were conducted. The antibacterial activity of nano-Ags was influenced by ATP-associated metabolism rather than by the permeability of the outer membrane. Additionally, nano-Ags generated hydroxyl radicals, a highly reactive oxygen species induced by bactericidal agents. It was concluded that nano-Ags have potential as a combination therapeutic agent for the treatment of infectious diseases by bacteria.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22956753</PMID><DateCompleted><Year>2013</Year><Month>01</Month><Day>18</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>04</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1473-5644</ISSN><JournalIssue CitedMedium="Internet"><Volume>61</Volume><Issue>Pt 12</Issue><PubDate><Year>2012</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Journal of medical microbiology</Title><ISOAbbreviation>J Med Microbiol</ISOAbbreviation></Journal><ArticleTitle>Synergistic effects between silver nanoparticles and antibiotics and the mechanisms involved.</ArticleTitle><Pagination><MedlinePgn>1719-1726</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1099/jmm.0.047100-0</ELocationID><Abstract><AbstractText>Silver nanoparticles (nano-Ags), which have well-known antimicrobial properties, are used extensively in various medical and general applications. In this study, the combination effects between nano-Ags and the conventional antibiotics ampicillin, chloramphenicol and kanamycin against various pathogenic bacteria were investigated. The MIC and fractional inhibitory concentration index (FICI) were determined to confirm antibacterial susceptibility and synergistic effects. The results showed that nano-Ags possessed antibacterial effects and synergistic activities. The antibiofilm activities of nano-Ags alone or in combination with antibiotics were also investigated. Formation of biofilm is associated with resistance to antimicrobial agents and chronic bacterial infections. The results indicated that nano-Ags also had antibiofilm activities. To understand these effects of nano-Ags, an ATPase inhibitor assay, permeability assay and hydroxyl radical assay were conducted. The antibacterial activity of nano-Ags was influenced by ATP-associated metabolism rather than by the permeability of the outer membrane. Additionally, nano-Ags generated hydroxyl radicals, a highly reactive oxygen species induced by bactericidal agents. It was concluded that nano-Ags have potential as a combination therapeutic agent for the treatment of infectious diseases by bacteria.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hwang</LastName><ForeName>In-Sok</ForeName><Initials>IS</Initials><AffiliationInfo><Affiliation>School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Daehak-ro 80 Buk-gu Daegu, 702-701, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hwang</LastName><ForeName>Ji Hong</ForeName><Initials>JH</Initials><AffiliationInfo><Affiliation>School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Daehak-ro 80 Buk-gu Daegu, 702-701, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Choi</LastName><ForeName>Hyemin</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Daehak-ro 80 Buk-gu Daegu, 702-701, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Keuk-Jun</ForeName><Initials>KJ</Initials><AffiliationInfo><Affiliation>Department of Clinical Pathology, Tae Kyeung College, 24, Danbuk-ri, Jain-myeon, Gyeongsan-si, Gyeongsangbuk-do, 712-719, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Dong Gun</ForeName><Initials>DG</Initials><AffiliationInfo><Affiliation>School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Daehak-ro 80 Buk-gu Daegu, 702-701, Republic of Korea.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>09</Month><Day>06</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Med Microbiol</MedlineTA><NlmUniqueID>0224131</NlmUniqueID><ISSNLinking>0022-2615</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000900">Anti-Bacterial Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>3M4G523W1G</RegistryNumber><NameOfSubstance UI="D012834">Silver</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000900" MajorTopicYN="N">Anti-Bacterial Agents</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001419" MajorTopicYN="N">Bacteria</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004357" MajorTopicYN="N">Drug Synergism</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016984" MajorTopicYN="N">Enterococcus faecium</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053768" MajorTopicYN="Y">Metal Nanoparticles</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008826" MajorTopicYN="N">Microbial Sensitivity Tests</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011550" MajorTopicYN="N">Pseudomonas aeruginosa</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012834" MajorTopicYN="N">Silver</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013211" MajorTopicYN="N">Staphylococcus aureus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013295" MajorTopicYN="N">Streptococcus mutans</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>9</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>9</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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