Pyocyanine Biosynthetic Genes in Clinical and Environmental Isolates of Pseudomonas aeruginosa and Detection of Pyocyanine's Antimicrobial Effects with or without Colloidal Silver Nanoparticles.
Identifieur interne : 000934 ( Main/Corpus ); précédent : 000933; suivant : 000935Pyocyanine Biosynthetic Genes in Clinical and Environmental Isolates of Pseudomonas aeruginosa and Detection of Pyocyanine's Antimicrobial Effects with or without Colloidal Silver Nanoparticles.
Auteurs : Jamileh Nowroozi ; Abbas Akhavan Sepahi ; Afrooz RashnonejadSource :
- Cell journal [ 2228-5806 ] ; 2012.
Abstract
OBJECTIVE
Pyocyanine plays an important role in the pathogenesis of Pseudomonas aeruginosa, (P. aeruginosa) and is known to have inhibitory and bactericidal effects. This study has aimed to detect the phenazine biosynthetic operon (phz ABCDEFG) and two phenazine modifying genes (phzM and phzS) by polymerase chain reaction (PCR) and detection of its possible protein bands by sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE). The antimicrobial effects of pyocyanine alone and mixed with colloidal silver nanoparticles were studied.
MATERIALS AND METHODS
In this descriptive study, clinical and environmental species of P. aeruginosa were isolated by thioglycollate medium culture and cetrimide agar, respectively. The existence of a phenazine biosynthetic operon and two phenazine modifying genes as well as their protein products were confirmed by PCR and SDS-PAGE, respectively. Pyocyanine was extracted with chloroform and its antimicrobial effects against bacteria such as; Escherichia coli (E. coli), P. aeruginosaand Staphylococcus aureus (S. aureus) bacteria and yeast Candida albicans (C. albicans) were tested using well, spot and disk diffusion methods.
RESULTS
In this study, 3 out of 48 clinical strains were unable to produce pyocyanine on cetrimide and Mueller Hinton (MH) agar. Two strains did not have phenazine modifying gene bands. Another strain did not have the possible protein band of the phzM gene. Pyocyanine had antimicrobial effects against the microbial strains, which increased in the presence of silver nanoparticles.
CONCLUSION
According to the results of the present study, some P. aeruginosa strains are unable to produce pyocyanine due to the absence of the phzM or phzS genes. Therefore, these genes have an important role in pyocyanine production in P. aeruginosa. Pyocyanine shows synergistic antimicrobial effects in the presence of silver nanoparticles against microbial strains.
PubMed: 23626932
PubMed Central: PMC3635824
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Pyocyanine Biosynthetic Genes in Clinical and Environmental Isolates of Pseudomonas aeruginosa and Detection of Pyocyanine's Antimicrobial Effects with or without Colloidal Silver Nanoparticles.</title><author><name sortKey="Nowroozi, Jamileh" sort="Nowroozi, Jamileh" uniqKey="Nowroozi J" first="Jamileh" last="Nowroozi">Jamileh Nowroozi</name><affiliation><nlm:affiliation>1. Department of Microbiology, Islamic Azad University, Tehran North Branch, Tehran, Iran.</nlm:affiliation></affiliation></author><author><name sortKey="Akhavan Sepahi, Abbas" sort="Akhavan Sepahi, Abbas" uniqKey="Akhavan Sepahi A" first="Abbas" last="Akhavan Sepahi">Abbas Akhavan Sepahi</name></author><author><name sortKey="Rashnonejad, Afrooz" sort="Rashnonejad, Afrooz" uniqKey="Rashnonejad A" first="Afrooz" last="Rashnonejad">Afrooz Rashnonejad</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:23626932</idno><idno type="pmid">23626932</idno><idno type="pmc">PMC3635824</idno><idno type="wicri:Area/Main/Corpus">000934</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000934</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Pyocyanine Biosynthetic Genes in Clinical and Environmental Isolates of Pseudomonas aeruginosa and Detection of Pyocyanine's Antimicrobial Effects with or without Colloidal Silver Nanoparticles.</title><author><name sortKey="Nowroozi, Jamileh" sort="Nowroozi, Jamileh" uniqKey="Nowroozi J" first="Jamileh" last="Nowroozi">Jamileh Nowroozi</name><affiliation><nlm:affiliation>1. Department of Microbiology, Islamic Azad University, Tehran North Branch, Tehran, Iran.</nlm:affiliation></affiliation></author><author><name sortKey="Akhavan Sepahi, Abbas" sort="Akhavan Sepahi, Abbas" uniqKey="Akhavan Sepahi A" first="Abbas" last="Akhavan Sepahi">Abbas Akhavan Sepahi</name></author><author><name sortKey="Rashnonejad, Afrooz" sort="Rashnonejad, Afrooz" uniqKey="Rashnonejad A" first="Afrooz" last="Rashnonejad">Afrooz Rashnonejad</name></author></analytic><series><title level="j">Cell journal</title><idno type="ISSN">2228-5806</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>OBJECTIVE</b></p><p>Pyocyanine plays an important role in the pathogenesis of Pseudomonas aeruginosa, (P. aeruginosa) and is known to have inhibitory and bactericidal effects. This study has aimed to detect the phenazine biosynthetic operon (phz ABCDEFG) and two phenazine modifying genes (phzM and phzS) by polymerase chain reaction (PCR) and detection of its possible protein bands by sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE). The antimicrobial effects of pyocyanine alone and mixed with colloidal silver nanoparticles were studied.</p></div><div type="abstract" xml:lang="en"><p><b>MATERIALS AND METHODS</b></p><p>In this descriptive study, clinical and environmental species of P. aeruginosa were isolated by thioglycollate medium culture and cetrimide agar, respectively. The existence of a phenazine biosynthetic operon and two phenazine modifying genes as well as their protein products were confirmed by PCR and SDS-PAGE, respectively. Pyocyanine was extracted with chloroform and its antimicrobial effects against bacteria such as; Escherichia coli (E. coli), P. aeruginosaand Staphylococcus aureus (S. aureus) bacteria and yeast Candida albicans (C. albicans) were tested using well, spot and disk diffusion methods.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>In this study, 3 out of 48 clinical strains were unable to produce pyocyanine on cetrimide and Mueller Hinton (MH) agar. Two strains did not have phenazine modifying gene bands. Another strain did not have the possible protein band of the phzM gene. Pyocyanine had antimicrobial effects against the microbial strains, which increased in the presence of silver nanoparticles.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSION</b></p><p>According to the results of the present study, some P. aeruginosa strains are unable to produce pyocyanine due to the absence of the phzM or phzS genes. Therefore, these genes have an important role in pyocyanine production in P. aeruginosa. Pyocyanine shows synergistic antimicrobial effects in the presence of silver nanoparticles against microbial strains.</p></div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">23626932</PMID><DateCompleted><Year>2013</Year><Month>04</Month><Day>30</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">2228-5806</ISSN><JournalIssue CitedMedium="Print"><Volume>14</Volume><Issue>1</Issue><PubDate><Year>2012</Year><Season>Spring</Season></PubDate></JournalIssue><Title>Cell journal</Title><ISOAbbreviation>Cell J</ISOAbbreviation></Journal><ArticleTitle>Pyocyanine Biosynthetic Genes in Clinical and Environmental Isolates of Pseudomonas aeruginosa and Detection of Pyocyanine's Antimicrobial Effects with or without Colloidal Silver Nanoparticles.</ArticleTitle><Pagination><MedlinePgn>7-18</MedlinePgn></Pagination><Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">Pyocyanine plays an important role in the pathogenesis of Pseudomonas aeruginosa, (P. aeruginosa) and is known to have inhibitory and bactericidal effects. This study has aimed to detect the phenazine biosynthetic operon (phz ABCDEFG) and two phenazine modifying genes (phzM and phzS) by polymerase chain reaction (PCR) and detection of its possible protein bands by sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE). The antimicrobial effects of pyocyanine alone and mixed with colloidal silver nanoparticles were studied.</AbstractText><AbstractText Label="MATERIALS AND METHODS" NlmCategory="METHODS">In this descriptive study, clinical and environmental species of P. aeruginosa were isolated by thioglycollate medium culture and cetrimide agar, respectively. The existence of a phenazine biosynthetic operon and two phenazine modifying genes as well as their protein products were confirmed by PCR and SDS-PAGE, respectively. Pyocyanine was extracted with chloroform and its antimicrobial effects against bacteria such as; Escherichia coli (E. coli), P. aeruginosaand Staphylococcus aureus (S. aureus) bacteria and yeast Candida albicans (C. albicans) were tested using well, spot and disk diffusion methods.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">In this study, 3 out of 48 clinical strains were unable to produce pyocyanine on cetrimide and Mueller Hinton (MH) agar. Two strains did not have phenazine modifying gene bands. Another strain did not have the possible protein band of the phzM gene. Pyocyanine had antimicrobial effects against the microbial strains, which increased in the presence of silver nanoparticles.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">According to the results of the present study, some P. aeruginosa strains are unable to produce pyocyanine due to the absence of the phzM or phzS genes. Therefore, these genes have an important role in pyocyanine production in P. aeruginosa. Pyocyanine shows synergistic antimicrobial effects in the presence of silver nanoparticles against microbial strains.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Nowroozi</LastName><ForeName>Jamileh</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>1. Department of Microbiology, Islamic Azad University, Tehran North Branch, Tehran, Iran.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Akhavan Sepahi</LastName><ForeName>Abbas</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Rashnonejad</LastName><ForeName>Afrooz</ForeName><Initials>A</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>06</Month><Day>13</Day></ArticleDate></Article><MedlineJournalInfo><Country>Iran</Country><MedlineTA>Cell J</MedlineTA><NlmUniqueID>101566618</NlmUniqueID><ISSNLinking>2228-5806</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Colloidal Silver Nanoparticles</Keyword><Keyword MajorTopicYN="N">Phenazine Modifying Gene</Keyword><Keyword MajorTopicYN="N">Pseudomonas aeruginosa</Keyword><Keyword 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