Green synthesis of silver nanoparticles using glucan from mushroom and study of antibacterial activity.
Identifieur interne : 000897 ( Main/Corpus ); précédent : 000896; suivant : 000898Green synthesis of silver nanoparticles using glucan from mushroom and study of antibacterial activity.
Auteurs : Ipsita Kumar Sen ; Amit Kumar Mandal ; Soumyananda Chakraborti ; Biswajit Dey ; Ranadhir Chakraborty ; Syed Sirajul IslamSource :
- International journal of biological macromolecules [ 1879-0003 ] ; 2013.
English descriptors
- KwdEn :
- Agaricales (chemistry), Anti-Bacterial Agents (administration & dosage), Anti-Bacterial Agents (chemistry), Antioxidants (pharmacology), Bacterial Load (MeSH), DNA Damage (drug effects), Drug Synergism (MeSH), Glucans (administration & dosage), Glucans (chemistry), Hemolysis (drug effects), Metal Nanoparticles (chemistry), Metal Nanoparticles (ultrastructure), Microbial Sensitivity Tests (MeSH), Silver (chemistry), Spectroscopy, Fourier Transform Infrared (MeSH), X-Ray Diffraction (MeSH).
- MESH :
- chemical , administration & dosage : Anti-Bacterial Agents, Glucans.
- chemistry : Agaricales, Anti-Bacterial Agents, Glucans, Metal Nanoparticles, Silver.
- drug effects : DNA Damage, Hemolysis.
- chemical , pharmacology : Antioxidants.
- ultrastructure : Metal Nanoparticles.
- Bacterial Load, Drug Synergism, Microbial Sensitivity Tests, Spectroscopy, Fourier Transform Infrared, X-Ray Diffraction.
Abstract
This work demonstrates synthesis of silver nanoparticles (AgNPs) using glucan isolated from a mushroom Pleurotus florida blue variant. UV-vis spectroscopy showed maximum absorbance at 425 nm due to surface plasmon resonance of AgNPs. Average diameter of the synthesized AgNPs was 2.445 ± 1.08 nm as revealed from TEM analysis. XRD analysis confirmed the face-centered cubic (fcc) crystalline structure of metallic silver. The synthesized AgNPs-glucan conjugates exhibited antibacterial activity against multiple antibiotic resistant (MAR) bacterium Klebsiella pneumoniae YSI6A and the activity was possibly due to damage of cellular macromolecules by the generation of reactive oxygen species (ROS) which was supported by observed degradation of bacterial DNA. Decrease of bactericidal effect of AgNPs-glucan conjugates in dose-dependent manner in presence of a ROS scavenger histidine further ascertained the involvement of ROS in antibacterial activity. AgNPs-glucan conjugates at LD50 dose caused least damage (0.68% hemolysis) to human RBCs. This particular dose of AgNPs-glucan conjugates in combination with each of the four antibiotics (ampicillin, azithromycin, cefepime and tetracycline) to which K. pneumoniae YSI6A was resistant, showed synergistic effect to inhibit almost 100% bacterial growth. It thus opens an avenue to use antibiotics in combination with minimum dosages of AgNPs-glucan conjugates to control MAR bacteria.
DOI: 10.1016/j.ijbiomac.2013.09.019
PubMed: 24076192
Links to Exploration step
pubmed:24076192Le document en format XML
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Ranadhir" sort="Chakraborty, Ranadhir" uniqKey="Chakraborty R" first="Ranadhir" last="Chakraborty">Ranadhir Chakraborty</name></author><author><name sortKey="Islam, Syed Sirajul" sort="Islam, Syed Sirajul" uniqKey="Islam S" first="Syed Sirajul" last="Islam">Syed Sirajul Islam</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:24076192</idno><idno type="pmid">24076192</idno><idno type="doi">10.1016/j.ijbiomac.2013.09.019</idno><idno type="wicri:Area/Main/Corpus">000897</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000897</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Green synthesis of silver nanoparticles using glucan from mushroom and study of antibacterial activity.</title><author><name sortKey="Sen, Ipsita Kumar" sort="Sen, Ipsita Kumar" uniqKey="Sen I" first="Ipsita Kumar" last="Sen">Ipsita Kumar Sen</name><affiliation><nlm:affiliation>Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.</nlm:affiliation></affiliation></author><author><name sortKey="Mandal, Amit Kumar" sort="Mandal, Amit Kumar" uniqKey="Mandal A" first="Amit Kumar" last="Mandal">Amit Kumar Mandal</name></author><author><name sortKey="Chakraborti, Soumyananda" sort="Chakraborti, Soumyananda" uniqKey="Chakraborti S" first="Soumyananda" last="Chakraborti">Soumyananda Chakraborti</name></author><author><name sortKey="Dey, Biswajit" sort="Dey, Biswajit" uniqKey="Dey B" first="Biswajit" last="Dey">Biswajit Dey</name></author><author><name sortKey="Chakraborty, Ranadhir" sort="Chakraborty, Ranadhir" uniqKey="Chakraborty R" first="Ranadhir" last="Chakraborty">Ranadhir Chakraborty</name></author><author><name sortKey="Islam, Syed Sirajul" sort="Islam, Syed Sirajul" uniqKey="Islam S" first="Syed Sirajul" last="Islam">Syed Sirajul Islam</name></author></analytic><series><title level="j">International journal of biological macromolecules</title><idno type="eISSN">1879-0003</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agaricales (chemistry)</term><term>Anti-Bacterial Agents (administration & dosage)</term><term>Anti-Bacterial Agents (chemistry)</term><term>Antioxidants (pharmacology)</term><term>Bacterial Load (MeSH)</term><term>DNA Damage (drug effects)</term><term>Drug Synergism (MeSH)</term><term>Glucans (administration & dosage)</term><term>Glucans (chemistry)</term><term>Hemolysis (drug effects)</term><term>Metal Nanoparticles (chemistry)</term><term>Metal Nanoparticles (ultrastructure)</term><term>Microbial Sensitivity Tests (MeSH)</term><term>Silver (chemistry)</term><term>Spectroscopy, Fourier Transform Infrared (MeSH)</term><term>X-Ray Diffraction (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Anti-Bacterial Agents</term><term>Glucans</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Agaricales</term><term>Anti-Bacterial Agents</term><term>Glucans</term><term>Metal Nanoparticles</term><term>Silver</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>DNA Damage</term><term>Hemolysis</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antioxidants</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Metal Nanoparticles</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Bacterial Load</term><term>Drug Synergism</term><term>Microbial Sensitivity Tests</term><term>Spectroscopy, Fourier Transform Infrared</term><term>X-Ray Diffraction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This work demonstrates synthesis of silver nanoparticles (AgNPs) using glucan isolated from a mushroom Pleurotus florida blue variant. UV-vis spectroscopy showed maximum absorbance at 425 nm due to surface plasmon resonance of AgNPs. Average diameter of the synthesized AgNPs was 2.445 ± 1.08 nm as revealed from TEM analysis. XRD analysis confirmed the face-centered cubic (fcc) crystalline structure of metallic silver. The synthesized AgNPs-glucan conjugates exhibited antibacterial activity against multiple antibiotic resistant (MAR) bacterium Klebsiella pneumoniae YSI6A and the activity was possibly due to damage of cellular macromolecules by the generation of reactive oxygen species (ROS) which was supported by observed degradation of bacterial DNA. Decrease of bactericidal effect of AgNPs-glucan conjugates in dose-dependent manner in presence of a ROS scavenger histidine further ascertained the involvement of ROS in antibacterial activity. AgNPs-glucan conjugates at LD50 dose caused least damage (0.68% hemolysis) to human RBCs. This particular dose of AgNPs-glucan conjugates in combination with each of the four antibiotics (ampicillin, azithromycin, cefepime and tetracycline) to which K. pneumoniae YSI6A was resistant, showed synergistic effect to inhibit almost 100% bacterial growth. It thus opens an avenue to use antibiotics in combination with minimum dosages of AgNPs-glucan conjugates to control MAR bacteria.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24076192</PMID><DateCompleted><Year>2014</Year><Month>07</Month><Day>18</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>22</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-0003</ISSN><JournalIssue CitedMedium="Internet"><Volume>62</Volume><PubDate><Year>2013</Year><Month>Nov</Month></PubDate></JournalIssue><Title>International journal of biological macromolecules</Title><ISOAbbreviation>Int J Biol Macromol</ISOAbbreviation></Journal><ArticleTitle>Green synthesis of silver nanoparticles using glucan from mushroom and study of antibacterial activity.</ArticleTitle><Pagination><MedlinePgn>439-49</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.ijbiomac.2013.09.019</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0141-8130(13)00503-5</ELocationID><Abstract><AbstractText>This work demonstrates synthesis of silver nanoparticles (AgNPs) using glucan isolated from a mushroom Pleurotus florida blue variant. UV-vis spectroscopy showed maximum absorbance at 425 nm due to surface plasmon resonance of AgNPs. Average diameter of the synthesized AgNPs was 2.445 ± 1.08 nm as revealed from TEM analysis. XRD analysis confirmed the face-centered cubic (fcc) crystalline structure of metallic silver. The synthesized AgNPs-glucan conjugates exhibited antibacterial activity against multiple antibiotic resistant (MAR) bacterium Klebsiella pneumoniae YSI6A and the activity was possibly due to damage of cellular macromolecules by the generation of reactive oxygen species (ROS) which was supported by observed degradation of bacterial DNA. Decrease of bactericidal effect of AgNPs-glucan conjugates in dose-dependent manner in presence of a ROS scavenger histidine further ascertained the involvement of ROS in antibacterial activity. AgNPs-glucan conjugates at LD50 dose caused least damage (0.68% hemolysis) to human RBCs. This particular dose of AgNPs-glucan conjugates in combination with each of the four antibiotics (ampicillin, azithromycin, cefepime and tetracycline) to which K. pneumoniae YSI6A was resistant, showed synergistic effect to inhibit almost 100% bacterial growth. It thus opens an avenue to use antibiotics in combination with minimum dosages of AgNPs-glucan conjugates to control MAR bacteria.</AbstractText><CopyrightInformation>Copyright © 2013 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Sen</LastName><ForeName>Ipsita Kumar</ForeName><Initials>IK</Initials><AffiliationInfo><Affiliation>Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mandal</LastName><ForeName>Amit Kumar</ForeName><Initials>AK</Initials></Author><Author ValidYN="Y"><LastName>Chakraborti</LastName><ForeName>Soumyananda</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Dey</LastName><ForeName>Biswajit</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Chakraborty</LastName><ForeName>Ranadhir</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Islam</LastName><ForeName>Syed Sirajul</ForeName><Initials>SS</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType 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effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004357" MajorTopicYN="N">Drug Synergism</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005936" MajorTopicYN="N">Glucans</DescriptorName><QualifierName UI="Q000008" MajorTopicYN="Y">administration & dosage</QualifierName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006461" MajorTopicYN="N">Hemolysis</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053768" MajorTopicYN="N">Metal Nanoparticles</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008826" MajorTopicYN="N">Microbial Sensitivity Tests</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012834" MajorTopicYN="N">Silver</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017550" MajorTopicYN="N">Spectroscopy, Fourier Transform Infrared</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014961" MajorTopicYN="N">X-Ray Diffraction</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">(1→6)-β-d-Glucan</Keyword><Keyword MajorTopicYN="N">Silver nanoparticles</Keyword><Keyword MajorTopicYN="N">Synergistic effect</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>06</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2013</Year><Month>08</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>09</Month><Day>19</Day></PubMedPubDate><PubMedPubDate 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