Biosynthesis of silver nanoparticles using Bacillus subtilis EWP-46 cell-free extract and evaluation of its antibacterial activity.
Identifieur interne : 000865 ( Main/Corpus ); précédent : 000864; suivant : 000866Biosynthesis of silver nanoparticles using Bacillus subtilis EWP-46 cell-free extract and evaluation of its antibacterial activity.
Auteurs : Palanivel Velmurugan ; Mahudunan Iydroose ; Mohmed Hanifa Abdul Kader Mohideen ; Thankiah Selva Mohan ; Min Cho ; Byung-Taek OhSource :
- Bioprocess and biosystems engineering [ 1615-7605 ] ; 2014.
English descriptors
- KwdEn :
- Anti-Bacterial Agents (chemical synthesis), Anti-Bacterial Agents (chemistry), Anti-Bacterial Agents (pharmacology), Bacillus subtilis (chemistry), Cell-Free System (chemistry), Metal Nanoparticles (chemistry), Pseudomonas fluorescens (growth & development), Silver (chemistry), Silver (pharmacology), Staphylococcus aureus (growth & development).
- MESH :
- chemical , chemical synthesis : Anti-Bacterial Agents.
- chemical , chemistry : Anti-Bacterial Agents, Silver.
- chemical , pharmacology : Anti-Bacterial Agents, Silver.
- chemistry : Bacillus subtilis, Cell-Free System, Metal Nanoparticles.
- growth & development : Pseudomonas fluorescens, Staphylococcus aureus.
Abstract
This study highlights the ability of nitrate-reducing Bacillus subtilis EWP-46 cell-free extract used for preparation of silver nanoparticles (AgNPs) by reduction of silver ions into nano silver. The production of AgNPs was optimized with several parameters such as hydrogen ion concentration, temperature, silver ion (Ag(+) ion) and time. The maximum AgNPs production was achieved at pH 10.0, temperature 60 °C, 1.0 mM Ag(+) ion and 720 min. The UV-Vis spectrum showed surface plasmon resonance peak at 420 nm, energy-dispersive X-ray spectroscopy (SEM-EDX) spectra showed the presence of element silver in pure form. Atomic force microscopy (AFM) and transmission electron microscopy images illustrated the nanoparticle size, shape, and average particle size ranging from 10 to 20 nm. Fourier transform infrared spectroscopy provided the evidence for the presence of biomolecules responsible for the reduction of silver ion, and X-ray diffraction analysis confirmed that the obtained nanoparticles were in crystalline form. SDS-PAGE was performed to identify the proteins and its molecular mass in the purified nitrate reductase from the cell-free extract. In addition, the minimum inhibitory concentration and minimum bactericidal concentration of AgNPs were investigated against gram-negative (Pseudomonas fluorescens) and gram-positive (Staphylococcus aureus) bacteria.
DOI: 10.1007/s00449-014-1124-6
PubMed: 24569955
Links to Exploration step
pubmed:24569955Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Biosynthesis of silver nanoparticles using Bacillus subtilis EWP-46 cell-free extract and evaluation of its antibacterial activity.</title><author><name sortKey="Velmurugan, Palanivel" sort="Velmurugan, Palanivel" uniqKey="Velmurugan P" first="Palanivel" last="Velmurugan">Palanivel Velmurugan</name><affiliation><nlm:affiliation>Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Jeonbuk, 570-752, South Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Iydroose, Mahudunan" sort="Iydroose, Mahudunan" uniqKey="Iydroose M" first="Mahudunan" last="Iydroose">Mahudunan Iydroose</name></author><author><name sortKey="Mohideen, Mohmed Hanifa Abdul Kader" sort="Mohideen, Mohmed Hanifa Abdul Kader" uniqKey="Mohideen M" first="Mohmed Hanifa Abdul Kader" last="Mohideen">Mohmed Hanifa Abdul Kader Mohideen</name></author><author><name sortKey="Mohan, Thankiah Selva" sort="Mohan, Thankiah Selva" uniqKey="Mohan T" first="Thankiah Selva" last="Mohan">Thankiah Selva Mohan</name></author><author><name sortKey="Cho, Min" sort="Cho, Min" uniqKey="Cho M" first="Min" last="Cho">Min Cho</name></author><author><name sortKey="Oh, Byung Taek" sort="Oh, Byung Taek" uniqKey="Oh B" first="Byung-Taek" last="Oh">Byung-Taek Oh</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:24569955</idno><idno type="pmid">24569955</idno><idno type="doi">10.1007/s00449-014-1124-6</idno><idno type="wicri:Area/Main/Corpus">000865</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000865</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Biosynthesis of silver nanoparticles using Bacillus subtilis EWP-46 cell-free extract and evaluation of its antibacterial activity.</title><author><name sortKey="Velmurugan, Palanivel" sort="Velmurugan, Palanivel" uniqKey="Velmurugan P" first="Palanivel" last="Velmurugan">Palanivel Velmurugan</name><affiliation><nlm:affiliation>Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Jeonbuk, 570-752, South Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Iydroose, Mahudunan" sort="Iydroose, Mahudunan" uniqKey="Iydroose M" first="Mahudunan" last="Iydroose">Mahudunan Iydroose</name></author><author><name sortKey="Mohideen, Mohmed Hanifa Abdul Kader" sort="Mohideen, Mohmed Hanifa Abdul Kader" uniqKey="Mohideen M" first="Mohmed Hanifa Abdul Kader" last="Mohideen">Mohmed Hanifa Abdul Kader Mohideen</name></author><author><name sortKey="Mohan, Thankiah Selva" sort="Mohan, Thankiah Selva" uniqKey="Mohan T" first="Thankiah Selva" last="Mohan">Thankiah Selva Mohan</name></author><author><name sortKey="Cho, Min" sort="Cho, Min" uniqKey="Cho M" first="Min" last="Cho">Min Cho</name></author><author><name sortKey="Oh, Byung Taek" sort="Oh, Byung Taek" uniqKey="Oh B" first="Byung-Taek" last="Oh">Byung-Taek Oh</name></author></analytic><series><title level="j">Bioprocess and biosystems engineering</title><idno type="eISSN">1615-7605</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anti-Bacterial Agents (chemical synthesis)</term><term>Anti-Bacterial Agents (chemistry)</term><term>Anti-Bacterial Agents (pharmacology)</term><term>Bacillus subtilis (chemistry)</term><term>Cell-Free System (chemistry)</term><term>Metal Nanoparticles (chemistry)</term><term>Pseudomonas fluorescens (growth & development)</term><term>Silver (chemistry)</term><term>Silver (pharmacology)</term><term>Staphylococcus aureus (growth & development)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemical synthesis" xml:lang="en"><term>Anti-Bacterial Agents</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Anti-Bacterial Agents</term><term>Silver</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Anti-Bacterial Agents</term><term>Silver</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Bacillus subtilis</term><term>Cell-Free System</term><term>Metal Nanoparticles</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Pseudomonas fluorescens</term><term>Staphylococcus aureus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This study highlights the ability of nitrate-reducing Bacillus subtilis EWP-46 cell-free extract used for preparation of silver nanoparticles (AgNPs) by reduction of silver ions into nano silver. The production of AgNPs was optimized with several parameters such as hydrogen ion concentration, temperature, silver ion (Ag(+) ion) and time. The maximum AgNPs production was achieved at pH 10.0, temperature 60 °C, 1.0 mM Ag(+) ion and 720 min. The UV-Vis spectrum showed surface plasmon resonance peak at 420 nm, energy-dispersive X-ray spectroscopy (SEM-EDX) spectra showed the presence of element silver in pure form. Atomic force microscopy (AFM) and transmission electron microscopy images illustrated the nanoparticle size, shape, and average particle size ranging from 10 to 20 nm. Fourier transform infrared spectroscopy provided the evidence for the presence of biomolecules responsible for the reduction of silver ion, and X-ray diffraction analysis confirmed that the obtained nanoparticles were in crystalline form. SDS-PAGE was performed to identify the proteins and its molecular mass in the purified nitrate reductase from the cell-free extract. In addition, the minimum inhibitory concentration and minimum bactericidal concentration of AgNPs were investigated against gram-negative (Pseudomonas fluorescens) and gram-positive (Staphylococcus aureus) bacteria.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24569955</PMID><DateCompleted><Year>2015</Year><Month>03</Month><Day>30</Day></DateCompleted><DateRevised><Year>2014</Year><Month>07</Month><Day>25</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1615-7605</ISSN><JournalIssue CitedMedium="Internet"><Volume>37</Volume><Issue>8</Issue><PubDate><Year>2014</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Bioprocess and biosystems engineering</Title><ISOAbbreviation>Bioprocess Biosyst Eng</ISOAbbreviation></Journal><ArticleTitle>Biosynthesis of silver nanoparticles using Bacillus subtilis EWP-46 cell-free extract and evaluation of its antibacterial activity.</ArticleTitle><Pagination><MedlinePgn>1527-34</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00449-014-1124-6</ELocationID><Abstract><AbstractText>This study highlights the ability of nitrate-reducing Bacillus subtilis EWP-46 cell-free extract used for preparation of silver nanoparticles (AgNPs) by reduction of silver ions into nano silver. The production of AgNPs was optimized with several parameters such as hydrogen ion concentration, temperature, silver ion (Ag(+) ion) and time. The maximum AgNPs production was achieved at pH 10.0, temperature 60 °C, 1.0 mM Ag(+) ion and 720 min. The UV-Vis spectrum showed surface plasmon resonance peak at 420 nm, energy-dispersive X-ray spectroscopy (SEM-EDX) spectra showed the presence of element silver in pure form. Atomic force microscopy (AFM) and transmission electron microscopy images illustrated the nanoparticle size, shape, and average particle size ranging from 10 to 20 nm. Fourier transform infrared spectroscopy provided the evidence for the presence of biomolecules responsible for the reduction of silver ion, and X-ray diffraction analysis confirmed that the obtained nanoparticles were in crystalline form. SDS-PAGE was performed to identify the proteins and its molecular mass in the purified nitrate reductase from the cell-free extract. In addition, the minimum inhibitory concentration and minimum bactericidal concentration of AgNPs were investigated against gram-negative (Pseudomonas fluorescens) and gram-positive (Staphylococcus aureus) bacteria.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Velmurugan</LastName><ForeName>Palanivel</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Jeonbuk, 570-752, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Iydroose</LastName><ForeName>Mahudunan</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Mohideen</LastName><ForeName>Mohmed Hanifa Abdul Kader</ForeName><Initials>MH</Initials></Author><Author ValidYN="Y"><LastName>Mohan</LastName><ForeName>Thankiah Selva</ForeName><Initials>TS</Initials></Author><Author ValidYN="Y"><LastName>Cho</LastName><ForeName>Min</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Oh</LastName><ForeName>Byung-Taek</ForeName><Initials>BT</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>02</Month><Day>26</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Bioprocess Biosyst Eng</MedlineTA><NlmUniqueID>101088505</NlmUniqueID><ISSNLinking>1615-7591</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000900">Anti-Bacterial Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>3M4G523W1G</RegistryNumber><NameOfSubstance UI="D012834">Silver</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000900" MajorTopicYN="Y">Anti-Bacterial Agents</DescriptorName><QualifierName UI="Q000138" MajorTopicYN="N">chemical synthesis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001412" MajorTopicYN="N">Bacillus subtilis</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002474" MajorTopicYN="N">Cell-Free System</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053768" MajorTopicYN="N">Metal Nanoparticles</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011551" MajorTopicYN="N">Pseudomonas fluorescens</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012834" MajorTopicYN="Y">Silver</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013211" MajorTopicYN="N">Staphylococcus aureus</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>12</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>01</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>2</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>2</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>3</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24569955</ArticleId><ArticleId IdType="doi">10.1007/s00449-014-1124-6</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Terre/explor/SilverBacteriV1/Data/Main/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000865 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 000865 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Terre
|area= SilverBacteriV1
|flux= Main
|étape= Corpus
|type= RBID
|clé= pubmed:24569955
|texte= Biosynthesis of silver nanoparticles using Bacillus subtilis EWP-46 cell-free extract and evaluation of its antibacterial activity.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Corpus/RBID.i -Sk "pubmed:24569955" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a SilverBacteriV1
| This area was generated with Dilib version V0.6.38. |  |