The Molecular Mechanisms of the Antibacterial Effect of Picosecond Laser Generated Silver Nanoparticles and Their Toxicity to Human Cells.
Identifieur interne : 000630 ( Main/Corpus ); précédent : 000629; suivant : 000631The Molecular Mechanisms of the Antibacterial Effect of Picosecond Laser Generated Silver Nanoparticles and Their Toxicity to Human Cells.
Auteurs : Peri Korshed ; Lin Li ; Zhu Liu ; Tao WangSource :
- PloS one [ 1932-6203 ] ; 2016.
English descriptors
- KwdEn :
- Anti-Bacterial Agents (chemistry), Anti-Bacterial Agents (pharmacology), Cell Line (MeSH), Dose-Response Relationship, Drug (MeSH), Escherichia coli (drug effects), Gram-Negative Bacteria (drug effects), Gram-Negative Bacteria (metabolism), Gram-Positive Bacteria (drug effects), Gram-Positive Bacteria (metabolism), Humans (MeSH), Lasers (MeSH), Metal Nanoparticles (chemistry), Pseudomonas aeruginosa (drug effects), Reactive Oxygen Species (metabolism), Silver (chemistry), Silver (pharmacology), Staphylococcus aureus (drug effects), Surface Properties (MeSH).
- MESH :
- chemical , chemistry : Anti-Bacterial Agents, Silver.
- chemical , metabolism : Reactive Oxygen Species.
- chemical , pharmacology : Anti-Bacterial Agents, Silver.
- chemistry : Metal Nanoparticles.
- drug effects : Escherichia coli, Gram-Negative Bacteria, Gram-Positive Bacteria, Pseudomonas aeruginosa, Staphylococcus aureus.
- metabolism : Gram-Negative Bacteria, Gram-Positive Bacteria.
- Cell Line, Dose-Response Relationship, Drug, Humans, Lasers, Surface Properties.
Abstract
Silver nanoparticles (Ag NPs) are known to have antibacterial properties. They are commonly produced by chemical synthesis which involves the use of harmful reducing agents. Contras, the laser technique is able to generate high-purity Ag NPs in water with specified surface charge characteristics. In the past, the molecular mechanisms contributing to the bactericidal effects of Ag NPs have been investigated extensively, but little is known of the antibacterial and toxic effects and mechanisms involved in laser-generated Ag NPs. In the current study Ag NPs were generated by picosecond laser ablation. Their antibacterial activity was determined on the gram-negative bacteria E. coli and Pseudomonas aeruginosa, and the gram positive bacteria Staphylococcus aureus including the methicillin resistant strain MRSA. Results showed that the laser generated Ag NPs exhibited strong dose-dependent antibacterial activity against all the three bacterial strains tested. Using E.coli as a model system, the laser Ag NPs treatment induced significantly high levels of reactive oxygen species (ROS). These ROS did not include detectable hydroxyl radicals, suggesting for the first time the selective ROS induction in bacterial cells by laser generated Ag NPs. The increased ROS was accompanied by significantly reduced cellular glutathione, and increased lipid peroxidation and permeability, suggesting ROS related bacterial cell damage. The laser generated Ag NPs exhibited low toxicity (within 72 hours) to five types of human cells although a weak significant decrease in cell survival was observed for endothelial cells and the lung cells. We conclude that picosecond laser generated Ag NPs have a broad spectrum of antibacterial effects against microbes including MRSA with minimal human cell toxicity. The oxidative stress is likely the key mechanism underlying the bactericidal effect, which leads to lipid peroxidation, depletion of glutathione, DNA damages and eventual disintegration of the cell membrane.
DOI: 10.1371/journal.pone.0160078
PubMed: 27575485
PubMed Central: PMC5004859
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pubmed:27575485Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The Molecular Mechanisms of the Antibacterial Effect of Picosecond Laser Generated Silver Nanoparticles and Their Toxicity to Human Cells.</title><author><name sortKey="Korshed, Peri" sort="Korshed, Peri" uniqKey="Korshed P" first="Peri" last="Korshed">Peri Korshed</name><affiliation><nlm:affiliation>Faculty of Medical and Human Science, the University of Manchester, Manchester, M13 9PL, United Kingdom.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Lin" sort="Li, Lin" uniqKey="Li L" first="Lin" last="Li">Lin Li</name><affiliation><nlm:affiliation>Laser Processing Research Centre, School of Mechanical, Aerospace and Civil Engineering, the University of Manchester, Manchester, M60 IQD, United Kingdom.</nlm:affiliation></affiliation></author><author><name sortKey="Liu, Zhu" sort="Liu, Zhu" uniqKey="Liu Z" first="Zhu" last="Liu">Zhu Liu</name><affiliation><nlm:affiliation>Corrosion and Protection Centre, the Mill, School of Materials, The University of Manchester, Manchester, M13 9PL, United Kingdom.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Tao" sort="Wang, Tao" uniqKey="Wang T" first="Tao" last="Wang">Tao Wang</name><affiliation><nlm:affiliation>Faculty of Medical and Human Science, the University of Manchester, Manchester, M13 9PL, United Kingdom.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27575485</idno><idno type="pmid">27575485</idno><idno type="doi">10.1371/journal.pone.0160078</idno><idno type="pmc">PMC5004859</idno><idno type="wicri:Area/Main/Corpus">000630</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000630</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The Molecular Mechanisms of the Antibacterial Effect of Picosecond Laser Generated Silver Nanoparticles and Their Toxicity to Human Cells.</title><author><name sortKey="Korshed, Peri" sort="Korshed, Peri" uniqKey="Korshed P" first="Peri" last="Korshed">Peri Korshed</name><affiliation><nlm:affiliation>Faculty of Medical and Human Science, the University of Manchester, Manchester, M13 9PL, United Kingdom.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Lin" sort="Li, Lin" uniqKey="Li L" first="Lin" last="Li">Lin Li</name><affiliation><nlm:affiliation>Laser Processing Research Centre, School of Mechanical, Aerospace and Civil Engineering, the University of Manchester, Manchester, M60 IQD, United Kingdom.</nlm:affiliation></affiliation></author><author><name sortKey="Liu, Zhu" sort="Liu, Zhu" uniqKey="Liu Z" first="Zhu" last="Liu">Zhu Liu</name><affiliation><nlm:affiliation>Corrosion and Protection Centre, the Mill, School of Materials, The University of Manchester, Manchester, M13 9PL, United Kingdom.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Tao" sort="Wang, Tao" uniqKey="Wang T" first="Tao" last="Wang">Tao Wang</name><affiliation><nlm:affiliation>Faculty of Medical and Human Science, the University of Manchester, Manchester, M13 9PL, United Kingdom.</nlm:affiliation></affiliation></author></analytic><series><title level="j">PloS one</title><idno type="eISSN">1932-6203</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anti-Bacterial Agents (chemistry)</term><term>Anti-Bacterial Agents (pharmacology)</term><term>Cell Line (MeSH)</term><term>Dose-Response Relationship, Drug (MeSH)</term><term>Escherichia coli (drug effects)</term><term>Gram-Negative Bacteria (drug effects)</term><term>Gram-Negative Bacteria (metabolism)</term><term>Gram-Positive Bacteria (drug effects)</term><term>Gram-Positive Bacteria (metabolism)</term><term>Humans (MeSH)</term><term>Lasers (MeSH)</term><term>Metal Nanoparticles (chemistry)</term><term>Pseudomonas aeruginosa (drug effects)</term><term>Reactive Oxygen Species (metabolism)</term><term>Silver (chemistry)</term><term>Silver (pharmacology)</term><term>Staphylococcus aureus (drug effects)</term><term>Surface Properties (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Anti-Bacterial Agents</term><term>Silver</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Reactive Oxygen Species</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Anti-Bacterial Agents</term><term>Silver</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Metal Nanoparticles</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Escherichia coli</term><term>Gram-Negative Bacteria</term><term>Gram-Positive Bacteria</term><term>Pseudomonas aeruginosa</term><term>Staphylococcus aureus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Gram-Negative Bacteria</term><term>Gram-Positive Bacteria</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Line</term><term>Dose-Response Relationship, Drug</term><term>Humans</term><term>Lasers</term><term>Surface Properties</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Silver nanoparticles (Ag NPs) are known to have antibacterial properties. They are commonly produced by chemical synthesis which involves the use of harmful reducing agents. Contras, the laser technique is able to generate high-purity Ag NPs in water with specified surface charge characteristics. In the past, the molecular mechanisms contributing to the bactericidal effects of Ag NPs have been investigated extensively, but little is known of the antibacterial and toxic effects and mechanisms involved in laser-generated Ag NPs. In the current study Ag NPs were generated by picosecond laser ablation. Their antibacterial activity was determined on the gram-negative bacteria E. coli and Pseudomonas aeruginosa, and the gram positive bacteria Staphylococcus aureus including the methicillin resistant strain MRSA. Results showed that the laser generated Ag NPs exhibited strong dose-dependent antibacterial activity against all the three bacterial strains tested. Using E.coli as a model system, the laser Ag NPs treatment induced significantly high levels of reactive oxygen species (ROS). These ROS did not include detectable hydroxyl radicals, suggesting for the first time the selective ROS induction in bacterial cells by laser generated Ag NPs. The increased ROS was accompanied by significantly reduced cellular glutathione, and increased lipid peroxidation and permeability, suggesting ROS related bacterial cell damage. The laser generated Ag NPs exhibited low toxicity (within 72 hours) to five types of human cells although a weak significant decrease in cell survival was observed for endothelial cells and the lung cells. We conclude that picosecond laser generated Ag NPs have a broad spectrum of antibacterial effects against microbes including MRSA with minimal human cell toxicity. The oxidative stress is likely the key mechanism underlying the bactericidal effect, which leads to lipid peroxidation, depletion of glutathione, DNA damages and eventual disintegration of the cell membrane. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27575485</PMID><DateCompleted><Year>2017</Year><Month>08</Month><Day>22</Day></DateCompleted><DateRevised><Year>2019</Year><Month>02</Month><Day>12</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1932-6203</ISSN><JournalIssue CitedMedium="Internet"><Volume>11</Volume><Issue>8</Issue><PubDate><Year>2016</Year></PubDate></JournalIssue><Title>PloS one</Title><ISOAbbreviation>PLoS One</ISOAbbreviation></Journal><ArticleTitle>The Molecular Mechanisms of the Antibacterial Effect of Picosecond Laser Generated Silver Nanoparticles and Their Toxicity to Human Cells.</ArticleTitle><Pagination><MedlinePgn>e0160078</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pone.0160078</ELocationID><Abstract><AbstractText>Silver nanoparticles (Ag NPs) are known to have antibacterial properties. They are commonly produced by chemical synthesis which involves the use of harmful reducing agents. Contras, the laser technique is able to generate high-purity Ag NPs in water with specified surface charge characteristics. In the past, the molecular mechanisms contributing to the bactericidal effects of Ag NPs have been investigated extensively, but little is known of the antibacterial and toxic effects and mechanisms involved in laser-generated Ag NPs. In the current study Ag NPs were generated by picosecond laser ablation. Their antibacterial activity was determined on the gram-negative bacteria E. coli and Pseudomonas aeruginosa, and the gram positive bacteria Staphylococcus aureus including the methicillin resistant strain MRSA. Results showed that the laser generated Ag NPs exhibited strong dose-dependent antibacterial activity against all the three bacterial strains tested. Using E.coli as a model system, the laser Ag NPs treatment induced significantly high levels of reactive oxygen species (ROS). These ROS did not include detectable hydroxyl radicals, suggesting for the first time the selective ROS induction in bacterial cells by laser generated Ag NPs. The increased ROS was accompanied by significantly reduced cellular glutathione, and increased lipid peroxidation and permeability, suggesting ROS related bacterial cell damage. The laser generated Ag NPs exhibited low toxicity (within 72 hours) to five types of human cells although a weak significant decrease in cell survival was observed for endothelial cells and the lung cells. We conclude that picosecond laser generated Ag NPs have a broad spectrum of antibacterial effects against microbes including MRSA with minimal human cell toxicity. The oxidative stress is likely the key mechanism underlying the bactericidal effect, which leads to lipid peroxidation, depletion of glutathione, DNA damages and eventual disintegration of the cell membrane. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Korshed</LastName><ForeName>Peri</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Faculty of Medical and Human Science, the University of Manchester, Manchester, M13 9PL, United Kingdom.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Lin</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Laser Processing Research Centre, School of Mechanical, Aerospace and Civil Engineering, the University of Manchester, Manchester, M60 IQD, United Kingdom.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Zhu</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Corrosion and Protection Centre, the Mill, School of Materials, The University of Manchester, Manchester, M13 9PL, United Kingdom.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Tao</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Faculty of Medical and Human Science, the University of Manchester, Manchester, M13 9PL, United Kingdom.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><Agency>Wellcome Trust</Agency><Country>United Kingdom</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>08</Month><Day>30</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>PLoS One</MedlineTA><NlmUniqueID>101285081</NlmUniqueID><ISSNLinking>1932-6203</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000900">Anti-Bacterial Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017382">Reactive Oxygen Species</NameOfSubstance></Chemical><Chemical><RegistryNumber>3M4G523W1G</RegistryNumber><NameOfSubstance UI="D012834">Silver</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="ErratumIn"><RefSource>PLoS One. 2018 Aug 30;13(8):e0203636</RefSource><PMID Version="1">30161224</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D000900" MajorTopicYN="N">Anti-Bacterial Agents</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004305" MajorTopicYN="N">Dose-Response Relationship, Drug</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006090" MajorTopicYN="N">Gram-Negative Bacteria</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006094" MajorTopicYN="N">Gram-Positive Bacteria</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007834" MajorTopicYN="N">Lasers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053768" MajorTopicYN="N">Metal Nanoparticles</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011550" MajorTopicYN="N">Pseudomonas aeruginosa</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017382" MajorTopicYN="N">Reactive Oxygen Species</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012834" MajorTopicYN="N">Silver</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013211" 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