Antibacterial-nanocomposite bone filler based on silver nanoparticles and polysaccharides.
Identifieur interne : 000608 ( Main/Corpus ); précédent : 000607; suivant : 000609Antibacterial-nanocomposite bone filler based on silver nanoparticles and polysaccharides.
Auteurs : Davide Porrelli ; Andrea Travan ; Gianluca Turco ; Matteo Crosera ; Massimiliano Borgogna ; Ivan Donati ; Sergio Paoletti ; Gianpiero Adami ; Eleonora MarsichSource :
- Journal of tissue engineering and regenerative medicine [ 1932-7005 ] ; 2018.
English descriptors
- KwdEn :
- Anti-Bacterial Agents (pharmacology), Biofilms (drug effects), Bone and Bones (drug effects), Cell Death (drug effects), Cell Line, Tumor (MeSH), Cell Survival (drug effects), Humans (MeSH), Metal Nanoparticles (chemistry), Microbial Sensitivity Tests (MeSH), Microspheres (MeSH), Nanocomposites (chemistry), Nanocomposites (ultrastructure), Polysaccharides (pharmacology), Pseudomonas aeruginosa (drug effects), Pseudomonas aeruginosa (growth & development), Silver (pharmacology), Staphylococcus epidermidis (drug effects), Staphylococcus epidermidis (growth & development).
- MESH :
- chemical , pharmacology : Anti-Bacterial Agents, Polysaccharides, Silver.
- chemistry : Metal Nanoparticles, Nanocomposites.
- drug effects : Biofilms, Bone and Bones, Cell Death, Cell Survival, Pseudomonas aeruginosa, Staphylococcus epidermidis.
- growth & development : Pseudomonas aeruginosa, Staphylococcus epidermidis.
- ultrastructure : Nanocomposites.
- Cell Line, Tumor, Humans, Microbial Sensitivity Tests, Microspheres.
Abstract
Injectable bone fillers represent an attractive strategy for the treatment of bone defects. These injectable materials should be biocompatible, capable of supporting cell growth and possibly able to exert antibacterial effects. In this work, nanocomposite microbeads based on alginate, chitlac, hydroxyapatite and silver nanoparticles were prepared and characterized. The dried microbeads displayed a rapid swelling in contact with simulated body fluid and maintained their integrity for more than 30 days. The evaluation of silver leakage from the microbeads showed that the antibacterial metal is slowly released in saline solution, with less than 6% of silver released after 1 week. Antibacterial tests proved that the microbeads displayed bactericidal effects toward Staphylococcus aureus, Pseudomonas aeruginosa and Staphylococcus epidermidis, and were also able to damage pre-formed bacterial biofilms. On the other hand, the microbeads did not exert any cytotoxic effect towards osteoblast-like cells. After characterization of the microbeads bioactivity, a possible means to embed them in a fluid medium was explored in order to obtain an injectable paste. Upon suspension of the particles in alginate solution or alginate/hyaluronic acid mixtures, a homogenous and time-stable paste was obtained. Mechanical tests enabled to quantify the extrusion forces from surgical syringes, pointing out the proper injectability of the material. This novel antibacterial bone filler appears as a promising material for the treatment of bone defects, in particular when possible infections could compromise the bone-healing process. Copyright © 2016 John Wiley & Sons, Ltd.
DOI: 10.1002/term.2365
PubMed: 27860429
Links to Exploration step
pubmed:27860429Le document en format XML
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Turco</name><affiliation><nlm:affiliation>Department of Medicine, Surgery and Health Sciences, University of Trieste, Trieste, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Crosera, Matteo" sort="Crosera, Matteo" uniqKey="Crosera M" first="Matteo" last="Crosera">Matteo Crosera</name><affiliation><nlm:affiliation>Department Chemical and Pharmaceutical Science, University of Trieste, Trieste, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Borgogna, Massimiliano" sort="Borgogna, Massimiliano" uniqKey="Borgogna M" first="Massimiliano" last="Borgogna">Massimiliano Borgogna</name><affiliation><nlm:affiliation>Department of Life Sciences, University of Trieste, Trieste, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Donati, Ivan" sort="Donati, Ivan" uniqKey="Donati I" first="Ivan" last="Donati">Ivan Donati</name><affiliation><nlm:affiliation>Department of Life Sciences, University of Trieste, Trieste, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Paoletti, Sergio" sort="Paoletti, Sergio" uniqKey="Paoletti S" first="Sergio" last="Paoletti">Sergio Paoletti</name><affiliation><nlm:affiliation>Department of Life Sciences, University of Trieste, Trieste, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Adami, Gianpiero" sort="Adami, Gianpiero" uniqKey="Adami G" first="Gianpiero" last="Adami">Gianpiero Adami</name><affiliation><nlm:affiliation>Department Chemical and Pharmaceutical Science, University of Trieste, Trieste, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Marsich, Eleonora" sort="Marsich, Eleonora" uniqKey="Marsich E" first="Eleonora" last="Marsich">Eleonora Marsich</name><affiliation><nlm:affiliation>Department of Medicine, Surgery and Health Sciences, University of Trieste, Trieste, Italy.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date 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last="Travan">Andrea Travan</name><affiliation><nlm:affiliation>Department of Life Sciences, University of Trieste, Trieste, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Turco, Gianluca" sort="Turco, Gianluca" uniqKey="Turco G" first="Gianluca" last="Turco">Gianluca Turco</name><affiliation><nlm:affiliation>Department of Medicine, Surgery and Health Sciences, University of Trieste, Trieste, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Crosera, Matteo" sort="Crosera, Matteo" uniqKey="Crosera M" first="Matteo" last="Crosera">Matteo Crosera</name><affiliation><nlm:affiliation>Department Chemical and Pharmaceutical Science, University of Trieste, Trieste, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Borgogna, Massimiliano" sort="Borgogna, Massimiliano" uniqKey="Borgogna M" first="Massimiliano" last="Borgogna">Massimiliano Borgogna</name><affiliation><nlm:affiliation>Department of Life Sciences, University of Trieste, Trieste, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Donati, Ivan" sort="Donati, Ivan" uniqKey="Donati I" first="Ivan" last="Donati">Ivan Donati</name><affiliation><nlm:affiliation>Department of Life Sciences, University of Trieste, Trieste, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Paoletti, Sergio" sort="Paoletti, Sergio" uniqKey="Paoletti S" first="Sergio" last="Paoletti">Sergio Paoletti</name><affiliation><nlm:affiliation>Department of Life Sciences, University of Trieste, Trieste, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Adami, Gianpiero" sort="Adami, Gianpiero" uniqKey="Adami G" first="Gianpiero" last="Adami">Gianpiero Adami</name><affiliation><nlm:affiliation>Department Chemical and Pharmaceutical Science, University of Trieste, Trieste, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Marsich, Eleonora" sort="Marsich, Eleonora" uniqKey="Marsich E" first="Eleonora" last="Marsich">Eleonora Marsich</name><affiliation><nlm:affiliation>Department of Medicine, Surgery and Health Sciences, University of Trieste, Trieste, Italy.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Journal of tissue engineering and regenerative medicine</title><idno type="eISSN">1932-7005</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anti-Bacterial Agents (pharmacology)</term><term>Biofilms (drug effects)</term><term>Bone and Bones (drug effects)</term><term>Cell Death (drug effects)</term><term>Cell Line, Tumor (MeSH)</term><term>Cell Survival (drug effects)</term><term>Humans (MeSH)</term><term>Metal Nanoparticles (chemistry)</term><term>Microbial Sensitivity Tests (MeSH)</term><term>Microspheres (MeSH)</term><term>Nanocomposites (chemistry)</term><term>Nanocomposites (ultrastructure)</term><term>Polysaccharides (pharmacology)</term><term>Pseudomonas aeruginosa (drug effects)</term><term>Pseudomonas aeruginosa (growth & development)</term><term>Silver (pharmacology)</term><term>Staphylococcus epidermidis (drug effects)</term><term>Staphylococcus epidermidis (growth & development)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Anti-Bacterial Agents</term><term>Polysaccharides</term><term>Silver</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Metal Nanoparticles</term><term>Nanocomposites</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Biofilms</term><term>Bone and Bones</term><term>Cell Death</term><term>Cell Survival</term><term>Pseudomonas aeruginosa</term><term>Staphylococcus epidermidis</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Pseudomonas aeruginosa</term><term>Staphylococcus epidermidis</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Nanocomposites</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Line, Tumor</term><term>Humans</term><term>Microbial Sensitivity Tests</term><term>Microspheres</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Injectable bone fillers represent an attractive strategy for the treatment of bone defects. These injectable materials should be biocompatible, capable of supporting cell growth and possibly able to exert antibacterial effects. In this work, nanocomposite microbeads based on alginate, chitlac, hydroxyapatite and silver nanoparticles were prepared and characterized. The dried microbeads displayed a rapid swelling in contact with simulated body fluid and maintained their integrity for more than 30 days. The evaluation of silver leakage from the microbeads showed that the antibacterial metal is slowly released in saline solution, with less than 6% of silver released after 1 week. Antibacterial tests proved that the microbeads displayed bactericidal effects toward Staphylococcus aureus, Pseudomonas aeruginosa and Staphylococcus epidermidis, and were also able to damage pre-formed bacterial biofilms. On the other hand, the microbeads did not exert any cytotoxic effect towards osteoblast-like cells. After characterization of the microbeads bioactivity, a possible means to embed them in a fluid medium was explored in order to obtain an injectable paste. Upon suspension of the particles in alginate solution or alginate/hyaluronic acid mixtures, a homogenous and time-stable paste was obtained. Mechanical tests enabled to quantify the extrusion forces from surgical syringes, pointing out the proper injectability of the material. This novel antibacterial bone filler appears as a promising material for the treatment of bone defects, in particular when possible infections could compromise the bone-healing process. Copyright © 2016 John Wiley & Sons, Ltd.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27860429</PMID><DateCompleted><Year>2019</Year><Month>10</Month><Day>08</Day></DateCompleted><DateRevised><Year>2019</Year><Month>10</Month><Day>08</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1932-7005</ISSN><JournalIssue CitedMedium="Internet"><Volume>12</Volume><Issue>2</Issue><PubDate><Year>2018</Year><Month>02</Month></PubDate></JournalIssue><Title>Journal of tissue engineering and regenerative medicine</Title><ISOAbbreviation>J Tissue Eng Regen Med</ISOAbbreviation></Journal><ArticleTitle>Antibacterial-nanocomposite bone filler based on silver nanoparticles and polysaccharides.</ArticleTitle><Pagination><MedlinePgn>e747-e759</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/term.2365</ELocationID><Abstract><AbstractText>Injectable bone fillers represent an attractive strategy for the treatment of bone defects. These injectable materials should be biocompatible, capable of supporting cell growth and possibly able to exert antibacterial effects. In this work, nanocomposite microbeads based on alginate, chitlac, hydroxyapatite and silver nanoparticles were prepared and characterized. The dried microbeads displayed a rapid swelling in contact with simulated body fluid and maintained their integrity for more than 30 days. The evaluation of silver leakage from the microbeads showed that the antibacterial metal is slowly released in saline solution, with less than 6% of silver released after 1 week. Antibacterial tests proved that the microbeads displayed bactericidal effects toward Staphylococcus aureus, Pseudomonas aeruginosa and Staphylococcus epidermidis, and were also able to damage pre-formed bacterial biofilms. On the other hand, the microbeads did not exert any cytotoxic effect towards osteoblast-like cells. After characterization of the microbeads bioactivity, a possible means to embed them in a fluid medium was explored in order to obtain an injectable paste. Upon suspension of the particles in alginate solution or alginate/hyaluronic acid mixtures, a homogenous and time-stable paste was obtained. Mechanical tests enabled to quantify the extrusion forces from surgical syringes, pointing out the proper injectability of the material. This novel antibacterial bone filler appears as a promising material for the treatment of bone defects, in particular when possible infections could compromise the bone-healing process. Copyright © 2016 John Wiley & Sons, Ltd.</AbstractText><CopyrightInformation>Copyright © 2016 John Wiley & Sons, Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Porrelli</LastName><ForeName>Davide</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Department of Medicine, Surgery and Health Sciences, University of Trieste, Trieste, Italy.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Life Sciences, University of Trieste, Trieste, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Travan</LastName><ForeName>Andrea</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Life Sciences, University of Trieste, Trieste, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Turco</LastName><ForeName>Gianluca</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Department of Medicine, Surgery and Health Sciences, University of Trieste, Trieste, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Crosera</LastName><ForeName>Matteo</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department Chemical and Pharmaceutical Science, University of Trieste, Trieste, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Borgogna</LastName><ForeName>Massimiliano</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Life Sciences, University of Trieste, Trieste, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Donati</LastName><ForeName>Ivan</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Department of Life Sciences, University of Trieste, Trieste, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Paoletti</LastName><ForeName>Sergio</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Life Sciences, University of Trieste, Trieste, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Adami</LastName><ForeName>Gianpiero</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Department Chemical and Pharmaceutical Science, University of Trieste, Trieste, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Marsich</LastName><ForeName>Eleonora</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Department of Medicine, Surgery and Health Sciences, University of Trieste, Trieste, Italy.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>05</Month><Day>07</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Tissue Eng Regen Med</MedlineTA><NlmUniqueID>101308490</NlmUniqueID><ISSNLinking>1932-6254</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000900">Anti-Bacterial Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011134">Polysaccharides</NameOfSubstance></Chemical><Chemical><RegistryNumber>3M4G523W1G</RegistryNumber><NameOfSubstance UI="D012834">Silver</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000900" MajorTopicYN="N">Anti-Bacterial Agents</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018441" MajorTopicYN="N">Biofilms</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001842" MajorTopicYN="N">Bone and Bones</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016923" MajorTopicYN="N">Cell Death</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045744" MajorTopicYN="N">Cell Line, Tumor</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002470" MajorTopicYN="N">Cell Survival</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053768" MajorTopicYN="N">Metal Nanoparticles</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008826" MajorTopicYN="N">Microbial Sensitivity Tests</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008863" MajorTopicYN="N">Microspheres</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053761" MajorTopicYN="N">Nanocomposites</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011134" MajorTopicYN="N">Polysaccharides</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011550" MajorTopicYN="N">Pseudomonas aeruginosa</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012834" MajorTopicYN="N">Silver</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013212" MajorTopicYN="N">Staphylococcus epidermidis</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">antibacterial properties</Keyword><Keyword MajorTopicYN="Y">bone healing</Keyword><Keyword MajorTopicYN="Y">injectable fillers</Keyword><Keyword MajorTopicYN="Y">polysaccharides</Keyword><Keyword MajorTopicYN="Y">silver nanoparticles</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>03</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2016</Year><Month>10</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>11</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>11</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>10</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>11</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27860429</ArticleId><ArticleId IdType="doi">10.1002/term.2365</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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