Green synthesized silver nanoparticles by marine endophytic fungus Penicillium polonicum and its antibacterial efficacy against biofilm forming, multidrug-resistant Acinetobacter baumanii.
Identifieur interne : 000461 ( Main/Corpus ); précédent : 000460; suivant : 000462Green synthesized silver nanoparticles by marine endophytic fungus Penicillium polonicum and its antibacterial efficacy against biofilm forming, multidrug-resistant Acinetobacter baumanii.
Auteurs : Sahadevan Neethu ; Sebastian Jose Midhun ; E K Radhakrishnan ; Mathew JyothisSource :
- Microbial pathogenesis [ 1096-1208 ] ; 2018.
English descriptors
- KwdEn :
- Acinetobacter baumannii (drug effects), Acinetobacter baumannii (physiology), Anti-Bacterial Agents (metabolism), Anti-Bacterial Agents (pharmacology), Biofilms (growth & development), Chlorophyta (microbiology), Colony Count, Microbial (MeSH), Drug Resistance, Multiple, Bacterial (MeSH), Endophytes (isolation & purification), Endophytes (metabolism), Metal Nanoparticles (MeSH), Microbial Sensitivity Tests (MeSH), Microbial Viability (drug effects), Microscopy, Electron, Transmission (MeSH), Penicillium (isolation & purification), Penicillium (metabolism), Silver (metabolism), Silver (pharmacology), Spectrophotometry (MeSH), Spectroscopy, Fourier Transform Infrared (MeSH), Spectrum Analysis, Raman (MeSH).
- MESH :
- chemical , metabolism : Anti-Bacterial Agents, Silver.
- drug effects : Acinetobacter baumannii, Microbial Viability.
- growth & development : Biofilms.
- isolation & purification : Endophytes, Penicillium.
- metabolism : Endophytes, Penicillium.
- microbiology : Chlorophyta.
- chemical , pharmacology : Anti-Bacterial Agents, Silver.
- physiology : Acinetobacter baumannii.
- Colony Count, Microbial, Drug Resistance, Multiple, Bacterial, Metal Nanoparticles, Microbial Sensitivity Tests, Microscopy, Electron, Transmission, Spectrophotometry, Spectroscopy, Fourier Transform Infrared, Spectrum Analysis, Raman.
Abstract
Acinetobacter baumanii, a gram-negative, non-motile, encapsulated coccobacillus which causes infections worldwide. The objective of this study was to find a fungal strain that could be utilized to biosynthesize antibacterial silver nanoparticles (AgNPs) against Acinetobacter baumanii. The present investigation explains rapid and extracellular biosynthesis of silver nanoparticles by the algicolous endophytic fungus, Penicillium polonicum, isolated from the marine green alga Chetomorpha antennina. The obtained silver nanoparticles were characterized by UV-Vis spectroscopy, Raman spectroscopy, Fourier transformation infrared (FTIR), and Transmission electron microscopy (TEM). The SNPs showed a characteristic UV- visible peak at 430 nm with an average size of 10-15 nm. As evident from the FTIR and Raman spectra, possibly the protein components of fungal extract have caused the reduction of silver nitrate. Parametric optimization, including the concentration of AgNO3, ratio of cell filtrate and AgNO3, fungal biomass, reaction time, pH, and presence of light, was done for rapid AgNPs production. The antibacterial efficacy of AgNPs against multi-drug-resistant, biofilm-forming Acinetobacter baumanii, was evaluated by well diffusion assay. The Minimum inhibitory concentration (MIC) of AgNP was 15.62 μgml-1 and the minimum bactericidal concentration (MBC) was 31.24 μgml-1. Killing kinetic assay revealed complete killing of the bacterial cells within 6 h. Log reduction and percent survival of bacterial cells were analyzed from killing kinetic study. Bactericidal nature of synthesized nanoparticles was confirmed by fluorescent microscopical analysis. The effect of AgNPs on the ultrastructure of bacterial pathogen was evaluated by Transmission electron microscopy.
DOI: 10.1016/j.micpath.2018.01.033
PubMed: 29366864
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Electronic address: jyothismathews@mgu.ac.in.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:29366864</idno><idno type="pmid">29366864</idno><idno type="doi">10.1016/j.micpath.2018.01.033</idno><idno type="wicri:Area/Main/Corpus">000461</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000461</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Green synthesized silver nanoparticles by marine endophytic fungus Penicillium polonicum and its antibacterial efficacy against biofilm forming, multidrug-resistant Acinetobacter baumanii.</title><author><name sortKey="Neethu, Sahadevan" sort="Neethu, Sahadevan" uniqKey="Neethu S" first="Sahadevan" last="Neethu">Sahadevan Neethu</name><affiliation><nlm:affiliation>School of BioSciences, Mahatma Gandhi University, Kottayam, Kerala, India.</nlm:affiliation></affiliation></author><author><name sortKey="Midhun, Sebastian Jose" sort="Midhun, Sebastian Jose" uniqKey="Midhun S" first="Sebastian Jose" last="Midhun">Sebastian Jose Midhun</name><affiliation><nlm:affiliation>School of BioSciences, Mahatma Gandhi University, Kottayam, Kerala, India.</nlm:affiliation></affiliation></author><author><name sortKey="Radhakrishnan, E K" sort="Radhakrishnan, E K" uniqKey="Radhakrishnan E" first="E K" last="Radhakrishnan">E K Radhakrishnan</name><affiliation><nlm:affiliation>School of BioSciences, Mahatma Gandhi University, Kottayam, Kerala, India.</nlm:affiliation></affiliation></author><author><name sortKey="Jyothis, Mathew" sort="Jyothis, Mathew" uniqKey="Jyothis M" first="Mathew" last="Jyothis">Mathew Jyothis</name><affiliation><nlm:affiliation>School of BioSciences, Mahatma Gandhi University, Kottayam, Kerala, India. Electronic address: jyothismathews@mgu.ac.in.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Microbial pathogenesis</title><idno type="eISSN">1096-1208</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acinetobacter baumannii (drug effects)</term><term>Acinetobacter baumannii (physiology)</term><term>Anti-Bacterial Agents (metabolism)</term><term>Anti-Bacterial Agents (pharmacology)</term><term>Biofilms (growth & development)</term><term>Chlorophyta (microbiology)</term><term>Colony Count, Microbial (MeSH)</term><term>Drug Resistance, Multiple, Bacterial (MeSH)</term><term>Endophytes (isolation & purification)</term><term>Endophytes (metabolism)</term><term>Metal Nanoparticles (MeSH)</term><term>Microbial Sensitivity Tests (MeSH)</term><term>Microbial Viability (drug effects)</term><term>Microscopy, Electron, Transmission (MeSH)</term><term>Penicillium (isolation & purification)</term><term>Penicillium (metabolism)</term><term>Silver (metabolism)</term><term>Silver (pharmacology)</term><term>Spectrophotometry (MeSH)</term><term>Spectroscopy, Fourier Transform Infrared (MeSH)</term><term>Spectrum Analysis, Raman (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Anti-Bacterial Agents</term><term>Silver</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Acinetobacter baumannii</term><term>Microbial Viability</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Biofilms</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Endophytes</term><term>Penicillium</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Endophytes</term><term>Penicillium</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Chlorophyta</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Anti-Bacterial Agents</term><term>Silver</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Acinetobacter baumannii</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Colony Count, Microbial</term><term>Drug Resistance, Multiple, Bacterial</term><term>Metal Nanoparticles</term><term>Microbial Sensitivity Tests</term><term>Microscopy, Electron, Transmission</term><term>Spectrophotometry</term><term>Spectroscopy, Fourier Transform Infrared</term><term>Spectrum Analysis, Raman</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Acinetobacter baumanii, a gram-negative, non-motile, encapsulated coccobacillus which causes infections worldwide. The objective of this study was to find a fungal strain that could be utilized to biosynthesize antibacterial silver nanoparticles (AgNPs) against Acinetobacter baumanii. The present investigation explains rapid and extracellular biosynthesis of silver nanoparticles by the algicolous endophytic fungus, Penicillium polonicum, isolated from the marine green alga Chetomorpha antennina. The obtained silver nanoparticles were characterized by UV-Vis spectroscopy, Raman spectroscopy, Fourier transformation infrared (FTIR), and Transmission electron microscopy (TEM). The SNPs showed a characteristic UV- visible peak at 430 nm with an average size of 10-15 nm. As evident from the FTIR and Raman spectra, possibly the protein components of fungal extract have caused the reduction of silver nitrate. Parametric optimization, including the concentration of AgNO<sub>3</sub>, ratio of cell filtrate and AgNO<sub>3</sub>, fungal biomass, reaction time, pH, and presence of light, was done for rapid AgNPs production. The antibacterial efficacy of AgNPs against multi-drug-resistant, biofilm-forming Acinetobacter baumanii, was evaluated by well diffusion assay. The Minimum inhibitory concentration (MIC) of AgNP was 15.62 μgml<sup>-1</sup> and the minimum bactericidal concentration (MBC) was 31.24 μgml<sup>-1</sup>. Killing kinetic assay revealed complete killing of the bacterial cells within 6 h. Log reduction and percent survival of bacterial cells were analyzed from killing kinetic study. Bactericidal nature of synthesized nanoparticles was confirmed by fluorescent microscopical analysis. The effect of AgNPs on the ultrastructure of bacterial pathogen was evaluated by Transmission electron microscopy.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29366864</PMID><DateCompleted><Year>2018</Year><Month>08</Month><Day>24</Day></DateCompleted><DateRevised><Year>2018</Year><Month>08</Month><Day>24</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1096-1208</ISSN><JournalIssue CitedMedium="Internet"><Volume>116</Volume><PubDate><Year>2018</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Microbial pathogenesis</Title><ISOAbbreviation>Microb Pathog</ISOAbbreviation></Journal><ArticleTitle>Green synthesized silver nanoparticles by marine endophytic fungus Penicillium polonicum and its antibacterial efficacy against biofilm forming, multidrug-resistant Acinetobacter baumanii.</ArticleTitle><Pagination><MedlinePgn>263-272</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0882-4010(17)31512-7</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.micpath.2018.01.033</ELocationID><Abstract><AbstractText>Acinetobacter baumanii, a gram-negative, non-motile, encapsulated coccobacillus which causes infections worldwide. The objective of this study was to find a fungal strain that could be utilized to biosynthesize antibacterial silver nanoparticles (AgNPs) against Acinetobacter baumanii. The present investigation explains rapid and extracellular biosynthesis of silver nanoparticles by the algicolous endophytic fungus, Penicillium polonicum, isolated from the marine green alga Chetomorpha antennina. The obtained silver nanoparticles were characterized by UV-Vis spectroscopy, Raman spectroscopy, Fourier transformation infrared (FTIR), and Transmission electron microscopy (TEM). The SNPs showed a characteristic UV- visible peak at 430 nm with an average size of 10-15 nm. As evident from the FTIR and Raman spectra, possibly the protein components of fungal extract have caused the reduction of silver nitrate. Parametric optimization, including the concentration of AgNO<sub>3</sub>, ratio of cell filtrate and AgNO<sub>3</sub>, fungal biomass, reaction time, pH, and presence of light, was done for rapid AgNPs production. The antibacterial efficacy of AgNPs against multi-drug-resistant, biofilm-forming Acinetobacter baumanii, was evaluated by well diffusion assay. The Minimum inhibitory concentration (MIC) of AgNP was 15.62 μgml<sup>-1</sup> and the minimum bactericidal concentration (MBC) was 31.24 μgml<sup>-1</sup>. Killing kinetic assay revealed complete killing of the bacterial cells within 6 h. Log reduction and percent survival of bacterial cells were analyzed from killing kinetic study. Bactericidal nature of synthesized nanoparticles was confirmed by fluorescent microscopical analysis. The effect of AgNPs on the ultrastructure of bacterial pathogen was evaluated by Transmission electron microscopy.</AbstractText><CopyrightInformation>Copyright © 2018 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Neethu</LastName><ForeName>Sahadevan</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>School of BioSciences, Mahatma Gandhi University, Kottayam, Kerala, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Midhun</LastName><ForeName>Sebastian Jose</ForeName><Initials>SJ</Initials><AffiliationInfo><Affiliation>School of BioSciences, Mahatma Gandhi University, Kottayam, Kerala, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Radhakrishnan</LastName><ForeName>E K</ForeName><Initials>EK</Initials><AffiliationInfo><Affiliation>School of BioSciences, Mahatma Gandhi University, Kottayam, Kerala, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jyothis</LastName><ForeName>Mathew</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>School of BioSciences, Mahatma Gandhi University, Kottayam, Kerala, India. Electronic address: jyothismathews@mgu.ac.in.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>01</Month><Day>31</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Microb Pathog</MedlineTA><NlmUniqueID>8606191</NlmUniqueID><ISSNLinking>0882-4010</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000900">Anti-Bacterial Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>3M4G523W1G</RegistryNumber><NameOfSubstance UI="D012834">Silver</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D040981" MajorTopicYN="N">Acinetobacter baumannii</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000900" MajorTopicYN="N">Anti-Bacterial Agents</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018441" MajorTopicYN="N">Biofilms</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000460" MajorTopicYN="N">Chlorophyta</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015169" MajorTopicYN="N">Colony Count, Microbial</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D024901" MajorTopicYN="N">Drug Resistance, Multiple, Bacterial</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D060026" MajorTopicYN="N">Endophytes</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053768" MajorTopicYN="Y">Metal Nanoparticles</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008826" MajorTopicYN="N">Microbial Sensitivity Tests</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D050296" MajorTopicYN="N">Microbial Viability</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D046529" MajorTopicYN="N">Microscopy, Electron, Transmission</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010407" MajorTopicYN="N">Penicillium</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012834" MajorTopicYN="N">Silver</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013053" MajorTopicYN="N">Spectrophotometry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017550" MajorTopicYN="N">Spectroscopy, Fourier Transform Infrared</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013059" MajorTopicYN="N">Spectrum Analysis, Raman</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">AgNP</Keyword><Keyword MajorTopicYN="N">Bactericidal efficacy</Keyword><Keyword MajorTopicYN="N">Biosynthesis</Keyword><Keyword MajorTopicYN="N">Characterization</Keyword><Keyword MajorTopicYN="N">Penicillium polonicum</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>11</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2018</Year><Month>01</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>01</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>1</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>8</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>1</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29366864</ArticleId><ArticleId IdType="pii">S0882-4010(17)31512-7</ArticleId><ArticleId IdType="doi">10.1016/j.micpath.2018.01.033</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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