Highly Stabilized Nanoparticles on Poly-l-Lysine-Coated Oxidized Metals: A Versatile Platform with Enhanced Antimicrobial Activity.
Identifieur interne : 000401 ( Main/Corpus ); précédent : 000400; suivant : 000402Highly Stabilized Nanoparticles on Poly-l-Lysine-Coated Oxidized Metals: A Versatile Platform with Enhanced Antimicrobial Activity.
Auteurs : Fiorela Ghilini ; Miriam C. Rodríguez González ; Alejandro G. Mi N ; Diego Pissinis ; Alberto Hernández Creus ; Roberto C. Salvarezza ; Patricia L. SchilardiSource :
- ACS applied materials & interfaces [ 1944-8252 ] ; 2018.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Polylysine.
- chemical : Anti-Bacterial Agents, Anti-Infective Agents, Silver.
- Metal Nanoparticles, Microbial Sensitivity Tests.
Abstract
The increasing incidence of infections in implantable devices has encouraged the search for biocompatible antimicrobial surfaces. To inhibit the bacterial adhesion and proliferation on biomaterials, several surface functionalization strategies have been developed. However, most of these strategies lead to bacteriostatic effect and only few of these are able to reach the bactericidal condition. In this work, bactericidal surfaces were designed through the functionalization of titanium surfaces with poly-l-lysine (PLL) as the mediator for the incorporation of antimicrobial silver nanoparticles (AgNPs). This functionalization influences the adsorption of the particles on the substrate impeding the agglomeration observed when bare titanium surfaces are used, leading to a homogeneous distribution of AgNPs on the surfaces. The antimicrobial activity of this surface has been tested against two different strains, namely, Staphylococcus aureus and Pseudomonas aeruginosa. For both strains and different AgNPs sizes, the surface modified with PLL and AgNPs shows a much enhanced antimicrobial activity in comparison with AgNPs deposited on bare titanium. This enhanced antibacterial activity is high enough to reach bactericidal effect, a condition hard to achieve in antimicrobial surfaces. Importantly, the designed surfaces are able to decrease the bacterial viability more than 5 orders with respect to the initial bacterial inoculum. That means that a relative low load of AgNPs on the PLL-modified titanium surfaces reaches 99.999% bacterial death after 24 h. The results of the present study are important to avoid infections in indwelling materials by reinforcing the preventive antibiotic therapy usually dosed throughout the surgical procedure and during the postoperative period.
DOI: 10.1021/acsami.8b07529
PubMed: 29927235
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Rodríguez González</name><affiliation><nlm:affiliation>Área de Química Física, Departamento de Química, Facultad de Ciencias , Universidad de La Laguna, Instituto de Materiales y Nanotecnología (IMN) , 38200 La Laguna , Tenerife , Spain.</nlm:affiliation></affiliation></author><author><name sortKey="Mi N, Alejandro G" sort="Mi N, Alejandro G" uniqKey="Mi N A" first="Alejandro G" last="Mi N">Alejandro G. Mi N</name><affiliation><nlm:affiliation>Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Facultad de Ciencias Exactas, UNLP-CONICET, CC16 Suc4 , La Plata 1900 , Buenos Aires , Argentina.</nlm:affiliation></affiliation></author><author><name sortKey="Pissinis, Diego" sort="Pissinis, Diego" uniqKey="Pissinis D" first="Diego" last="Pissinis">Diego Pissinis</name><affiliation><nlm:affiliation>Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Facultad de Ciencias Exactas, UNLP-CONICET, CC16 Suc4 , La Plata 1900 , Buenos Aires , Argentina.</nlm:affiliation></affiliation></author><author><name sortKey="Creus, Alberto Hernandez" sort="Creus, Alberto Hernandez" uniqKey="Creus A" first="Alberto Hernández" last="Creus">Alberto Hernández Creus</name><affiliation><nlm:affiliation>Área de Química Física, Departamento de Química, Facultad de Ciencias , Universidad de La Laguna, Instituto de Materiales y Nanotecnología (IMN) , 38200 La Laguna , Tenerife , Spain.</nlm:affiliation></affiliation></author><author><name sortKey="Salvarezza, Roberto C" sort="Salvarezza, Roberto C" uniqKey="Salvarezza R" first="Roberto C" last="Salvarezza">Roberto C. Salvarezza</name><affiliation><nlm:affiliation>Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Facultad de Ciencias Exactas, UNLP-CONICET, CC16 Suc4 , La Plata 1900 , Buenos Aires , Argentina.</nlm:affiliation></affiliation></author><author><name sortKey="Schilardi, Patricia L" sort="Schilardi, Patricia L" uniqKey="Schilardi P" first="Patricia L" last="Schilardi">Patricia L. Schilardi</name><affiliation><nlm:affiliation>Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Facultad de Ciencias Exactas, UNLP-CONICET, CC16 Suc4 , La Plata 1900 , Buenos Aires , Argentina.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:29927235</idno><idno type="pmid">29927235</idno><idno type="doi">10.1021/acsami.8b07529</idno><idno type="wicri:Area/Main/Corpus">000401</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000401</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Highly Stabilized Nanoparticles on Poly-l-Lysine-Coated Oxidized Metals: A Versatile Platform with Enhanced Antimicrobial Activity.</title><author><name sortKey="Ghilini, Fiorela" sort="Ghilini, Fiorela" uniqKey="Ghilini F" first="Fiorela" last="Ghilini">Fiorela Ghilini</name><affiliation><nlm:affiliation>Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Facultad de Ciencias Exactas, UNLP-CONICET, CC16 Suc4 , La Plata 1900 , Buenos Aires , Argentina.</nlm:affiliation></affiliation></author><author><name sortKey="Rodriguez Gonzalez, Miriam C" sort="Rodriguez Gonzalez, Miriam C" uniqKey="Rodriguez Gonzalez M" first="Miriam C" last="Rodríguez González">Miriam C. Rodríguez González</name><affiliation><nlm:affiliation>Área de Química Física, Departamento de Química, Facultad de Ciencias , Universidad de La Laguna, Instituto de Materiales y Nanotecnología (IMN) , 38200 La Laguna , Tenerife , Spain.</nlm:affiliation></affiliation></author><author><name sortKey="Mi N, Alejandro G" sort="Mi N, Alejandro G" uniqKey="Mi N A" first="Alejandro G" last="Mi N">Alejandro G. Mi N</name><affiliation><nlm:affiliation>Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Facultad de Ciencias Exactas, UNLP-CONICET, CC16 Suc4 , La Plata 1900 , Buenos Aires , Argentina.</nlm:affiliation></affiliation></author><author><name sortKey="Pissinis, Diego" sort="Pissinis, Diego" uniqKey="Pissinis D" first="Diego" last="Pissinis">Diego Pissinis</name><affiliation><nlm:affiliation>Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Facultad de Ciencias Exactas, UNLP-CONICET, CC16 Suc4 , La Plata 1900 , Buenos Aires , Argentina.</nlm:affiliation></affiliation></author><author><name sortKey="Creus, Alberto Hernandez" sort="Creus, Alberto Hernandez" uniqKey="Creus A" first="Alberto Hernández" last="Creus">Alberto Hernández Creus</name><affiliation><nlm:affiliation>Área de Química Física, Departamento de Química, Facultad de Ciencias , Universidad de La Laguna, Instituto de Materiales y Nanotecnología (IMN) , 38200 La Laguna , Tenerife , Spain.</nlm:affiliation></affiliation></author><author><name sortKey="Salvarezza, Roberto C" sort="Salvarezza, Roberto C" uniqKey="Salvarezza R" first="Roberto C" last="Salvarezza">Roberto C. Salvarezza</name><affiliation><nlm:affiliation>Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Facultad de Ciencias Exactas, UNLP-CONICET, CC16 Suc4 , La Plata 1900 , Buenos Aires , Argentina.</nlm:affiliation></affiliation></author><author><name sortKey="Schilardi, Patricia L" sort="Schilardi, Patricia L" uniqKey="Schilardi P" first="Patricia L" last="Schilardi">Patricia L. Schilardi</name><affiliation><nlm:affiliation>Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Facultad de Ciencias Exactas, UNLP-CONICET, CC16 Suc4 , La Plata 1900 , Buenos Aires , Argentina.</nlm:affiliation></affiliation></author></analytic><series><title level="j">ACS applied materials & interfaces</title><idno type="eISSN">1944-8252</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anti-Bacterial Agents (MeSH)</term><term>Anti-Infective Agents (MeSH)</term><term>Metal Nanoparticles (MeSH)</term><term>Microbial Sensitivity Tests (MeSH)</term><term>Polylysine (chemistry)</term><term>Silver (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Polylysine</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Anti-Bacterial Agents</term><term>Anti-Infective Agents</term><term>Silver</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Metal Nanoparticles</term><term>Microbial Sensitivity Tests</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The increasing incidence of infections in implantable devices has encouraged the search for biocompatible antimicrobial surfaces. To inhibit the bacterial adhesion and proliferation on biomaterials, several surface functionalization strategies have been developed. However, most of these strategies lead to bacteriostatic effect and only few of these are able to reach the bactericidal condition. In this work, bactericidal surfaces were designed through the functionalization of titanium surfaces with poly-l-lysine (PLL) as the mediator for the incorporation of antimicrobial silver nanoparticles (AgNPs). This functionalization influences the adsorption of the particles on the substrate impeding the agglomeration observed when bare titanium surfaces are used, leading to a homogeneous distribution of AgNPs on the surfaces. The antimicrobial activity of this surface has been tested against two different strains, namely, Staphylococcus aureus and Pseudomonas aeruginosa. For both strains and different AgNPs sizes, the surface modified with PLL and AgNPs shows a much enhanced antimicrobial activity in comparison with AgNPs deposited on bare titanium. This enhanced antibacterial activity is high enough to reach bactericidal effect, a condition hard to achieve in antimicrobial surfaces. Importantly, the designed surfaces are able to decrease the bacterial viability more than 5 orders with respect to the initial bacterial inoculum. That means that a relative low load of AgNPs on the PLL-modified titanium surfaces reaches 99.999% bacterial death after 24 h. The results of the present study are important to avoid infections in indwelling materials by reinforcing the preventive antibiotic therapy usually dosed throughout the surgical procedure and during the postoperative period.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">29927235</PMID><DateCompleted><Year>2019</Year><Month>02</Month><Day>13</Day></DateCompleted><DateRevised><Year>2019</Year><Month>02</Month><Day>15</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1944-8252</ISSN><JournalIssue CitedMedium="Internet"><Volume>10</Volume><Issue>28</Issue><PubDate><Year>2018</Year><Month>Jul</Month><Day>18</Day></PubDate></JournalIssue><Title>ACS applied materials & interfaces</Title><ISOAbbreviation>ACS Appl Mater Interfaces</ISOAbbreviation></Journal><ArticleTitle>Highly Stabilized Nanoparticles on Poly-l-Lysine-Coated Oxidized Metals: A Versatile Platform with Enhanced Antimicrobial Activity.</ArticleTitle><Pagination><MedlinePgn>23657-23666</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/acsami.8b07529</ELocationID><Abstract><AbstractText>The increasing incidence of infections in implantable devices has encouraged the search for biocompatible antimicrobial surfaces. To inhibit the bacterial adhesion and proliferation on biomaterials, several surface functionalization strategies have been developed. However, most of these strategies lead to bacteriostatic effect and only few of these are able to reach the bactericidal condition. In this work, bactericidal surfaces were designed through the functionalization of titanium surfaces with poly-l-lysine (PLL) as the mediator for the incorporation of antimicrobial silver nanoparticles (AgNPs). This functionalization influences the adsorption of the particles on the substrate impeding the agglomeration observed when bare titanium surfaces are used, leading to a homogeneous distribution of AgNPs on the surfaces. The antimicrobial activity of this surface has been tested against two different strains, namely, Staphylococcus aureus and Pseudomonas aeruginosa. For both strains and different AgNPs sizes, the surface modified with PLL and AgNPs shows a much enhanced antimicrobial activity in comparison with AgNPs deposited on bare titanium. This enhanced antibacterial activity is high enough to reach bactericidal effect, a condition hard to achieve in antimicrobial surfaces. Importantly, the designed surfaces are able to decrease the bacterial viability more than 5 orders with respect to the initial bacterial inoculum. That means that a relative low load of AgNPs on the PLL-modified titanium surfaces reaches 99.999% bacterial death after 24 h. The results of the present study are important to avoid infections in indwelling materials by reinforcing the preventive antibiotic therapy usually dosed throughout the surgical procedure and during the postoperative period.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ghilini</LastName><ForeName>Fiorela</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Facultad de Ciencias Exactas, UNLP-CONICET, CC16 Suc4 , La Plata 1900 , Buenos Aires , Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rodríguez González</LastName><ForeName>Miriam C</ForeName><Initials>MC</Initials><AffiliationInfo><Affiliation>Área de Química Física, Departamento de Química, Facultad de Ciencias , Universidad de La Laguna, Instituto de Materiales y Nanotecnología (IMN) , 38200 La Laguna , Tenerife , Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Miñán</LastName><ForeName>Alejandro G</ForeName><Initials>AG</Initials><AffiliationInfo><Affiliation>Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Facultad de Ciencias Exactas, UNLP-CONICET, CC16 Suc4 , La Plata 1900 , Buenos Aires , Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pissinis</LastName><ForeName>Diego</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Facultad de Ciencias Exactas, UNLP-CONICET, CC16 Suc4 , La Plata 1900 , Buenos Aires , Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Creus</LastName><ForeName>Alberto Hernández</ForeName><Initials>AH</Initials><AffiliationInfo><Affiliation>Área de Química Física, Departamento de Química, Facultad de Ciencias , Universidad de La Laguna, Instituto de Materiales y Nanotecnología (IMN) , 38200 La Laguna , Tenerife , Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Salvarezza</LastName><ForeName>Roberto C</ForeName><Initials>RC</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-7617-4539</Identifier><AffiliationInfo><Affiliation>Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Facultad de Ciencias Exactas, UNLP-CONICET, CC16 Suc4 , La Plata 1900 , Buenos Aires , Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schilardi</LastName><ForeName>Patricia L</ForeName><Initials>PL</Initials><Identifier Source="ORCID">http://orcid.org/0000-0003-1704-2867</Identifier><AffiliationInfo><Affiliation>Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Facultad de Ciencias Exactas, UNLP-CONICET, CC16 Suc4 , La Plata 1900 , Buenos Aires , Argentina.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>07</Month><Day>05</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>ACS Appl Mater Interfaces</MedlineTA><NlmUniqueID>101504991</NlmUniqueID><ISSNLinking>1944-8244</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000900">Anti-Bacterial Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000890">Anti-Infective Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>25104-18-1</RegistryNumber><NameOfSubstance UI="D011107">Polylysine</NameOfSubstance></Chemical><Chemical><RegistryNumber>3M4G523W1G</RegistryNumber><NameOfSubstance UI="D012834">Silver</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000900" MajorTopicYN="N">Anti-Bacterial Agents</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000890" MajorTopicYN="N">Anti-Infective Agents</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053768" MajorTopicYN="N">Metal Nanoparticles</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008826" MajorTopicYN="N">Microbial Sensitivity Tests</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011107" MajorTopicYN="N">Polylysine</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012834" MajorTopicYN="N">Silver</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">bactericidal</Keyword><Keyword MajorTopicYN="N">biofilms</Keyword><Keyword MajorTopicYN="N">poly-l-lysine</Keyword><Keyword MajorTopicYN="N">silver nanoparticles</Keyword><Keyword MajorTopicYN="N">titanium functionalization</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>6</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>2</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>6</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29927235</ArticleId><ArticleId IdType="doi">10.1021/acsami.8b07529</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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