Inactivation of bacteria in plasma by photosensitizers benzophenone and vitamins K3, B1 and B6 with UV A light irradiation.
Identifieur interne : 000143 ( Main/Corpus ); précédent : 000142; suivant : 000144Inactivation of bacteria in plasma by photosensitizers benzophenone and vitamins K3, B1 and B6 with UV A light irradiation.
Auteurs : Fei Xu ; Qinati Feyissa ; Zina Ibrahim ; Ying Li ; Kevin L. Xu ; Zihan Guo ; Justen Ahmad ; Jaroslav G. VostalSource :
- Photodiagnosis and photodynamic therapy [ 1873-1597 ] ; 2020.
Abstract
BACKGROUND
A photosensitizer is a light-activated molecule that can generate reactive oxygen species or directly interact with nucleic acids. Both consequences can be applied to reduction of pathogens in various media and to selectively attack tumor cells. Numerous natural and synthesized photosensitizers have been identified for pathogen reduction.
METHODS
The photosensitizers of vitamins K3 (VK3), B1 (VB1), B6 (VB6) and benzophenone (BP) were prepared in 100-200 μM of PBS solution, irradiated with UVA at 0-48 J/cm
RESULTS AND CONCLUSIONS
Over six logs CFU/ml reduction of E. coli suspended in PBS occurred after treatment with either VB1, VB6, VK3 or BP combined with UVA irradiation. When bacteria were suspended in plasma, two to seven logs reduction occurred depending on the UVA dose, photosensitizer concentration, and bacteria species. Among these photosensitizers, BP had the most potent bactericidal effect and is a promising UVA photosensitizer for pathogen reduction. The level of absorption spectrum alteration after UVA irradiation was profound for VK3 and VB6 but minimal for BP and VB1. The UV-vis absorption spectrum changes did not correlate with the bactericidal effect indicating that molecule modification by UVA light is not required for the bactericidal activity.
DOI: 10.1016/j.pdpdt.2020.101713
PubMed: 32165334
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Inactivation of bacteria in plasma by photosensitizers benzophenone and vitamins K3, B1 and B6 with UV A light irradiation.</title><author><name sortKey="Xu, Fei" sort="Xu, Fei" uniqKey="Xu F" first="Fei" last="Xu">Fei Xu</name><affiliation><nlm:affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, Center for Biologics Evaluation and Research, FDA, Silver Spring, MD, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Feyissa, Qinati" sort="Feyissa, Qinati" uniqKey="Feyissa Q" first="Qinati" last="Feyissa">Qinati Feyissa</name><affiliation><nlm:affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, Center for Biologics Evaluation and Research, FDA, Silver Spring, MD, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Ibrahim, Zina" sort="Ibrahim, Zina" uniqKey="Ibrahim Z" first="Zina" last="Ibrahim">Zina Ibrahim</name><affiliation><nlm:affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, Center for Biologics Evaluation and Research, FDA, Silver Spring, MD, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Ying" sort="Li, Ying" uniqKey="Li Y" first="Ying" last="Li">Ying Li</name><affiliation><nlm:affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, Center for Biologics Evaluation and Research, FDA, Silver Spring, MD, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Xu, Kevin L" sort="Xu, Kevin L" uniqKey="Xu K" first="Kevin L" last="Xu">Kevin L. Xu</name><affiliation><nlm:affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, Center for Biologics Evaluation and Research, FDA, Silver Spring, MD, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Guo, Zihan" sort="Guo, Zihan" uniqKey="Guo Z" first="Zihan" last="Guo">Zihan Guo</name><affiliation><nlm:affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, Center for Biologics Evaluation and Research, FDA, Silver Spring, MD, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Ahmad, Justen" sort="Ahmad, Justen" uniqKey="Ahmad J" first="Justen" last="Ahmad">Justen Ahmad</name><affiliation><nlm:affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, Center for Biologics Evaluation and Research, FDA, Silver Spring, MD, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Vostal, Jaroslav G" sort="Vostal, Jaroslav G" uniqKey="Vostal J" first="Jaroslav G" last="Vostal">Jaroslav G. Vostal</name><affiliation><nlm:affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, Center for Biologics Evaluation and Research, FDA, Silver Spring, MD, USA. Electronic address: Jaroslav.Vostal@fda.hhs.gov.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32165334</idno><idno type="pmid">32165334</idno><idno type="doi">10.1016/j.pdpdt.2020.101713</idno><idno type="wicri:Area/Main/Corpus">000143</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000143</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Inactivation of bacteria in plasma by photosensitizers benzophenone and vitamins K3, B1 and B6 with UV A light irradiation.</title><author><name sortKey="Xu, Fei" sort="Xu, Fei" uniqKey="Xu F" first="Fei" last="Xu">Fei Xu</name><affiliation><nlm:affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, Center for Biologics Evaluation and Research, FDA, Silver Spring, MD, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Feyissa, Qinati" sort="Feyissa, Qinati" uniqKey="Feyissa Q" first="Qinati" last="Feyissa">Qinati Feyissa</name><affiliation><nlm:affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, Center for Biologics Evaluation and Research, FDA, Silver Spring, MD, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Ibrahim, Zina" sort="Ibrahim, Zina" uniqKey="Ibrahim Z" first="Zina" last="Ibrahim">Zina Ibrahim</name><affiliation><nlm:affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, Center for Biologics Evaluation and Research, FDA, Silver Spring, MD, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Ying" sort="Li, Ying" uniqKey="Li Y" first="Ying" last="Li">Ying Li</name><affiliation><nlm:affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, Center for Biologics Evaluation and Research, FDA, Silver Spring, MD, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Xu, Kevin L" sort="Xu, Kevin L" uniqKey="Xu K" first="Kevin L" last="Xu">Kevin L. Xu</name><affiliation><nlm:affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, Center for Biologics Evaluation and Research, FDA, Silver Spring, MD, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Guo, Zihan" sort="Guo, Zihan" uniqKey="Guo Z" first="Zihan" last="Guo">Zihan Guo</name><affiliation><nlm:affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, Center for Biologics Evaluation and Research, FDA, Silver Spring, MD, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Ahmad, Justen" sort="Ahmad, Justen" uniqKey="Ahmad J" first="Justen" last="Ahmad">Justen Ahmad</name><affiliation><nlm:affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, Center for Biologics Evaluation and Research, FDA, Silver Spring, MD, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Vostal, Jaroslav G" sort="Vostal, Jaroslav G" uniqKey="Vostal J" first="Jaroslav G" last="Vostal">Jaroslav G. Vostal</name><affiliation><nlm:affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, Center for Biologics Evaluation and Research, FDA, Silver Spring, MD, USA. Electronic address: Jaroslav.Vostal@fda.hhs.gov.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Photodiagnosis and photodynamic therapy</title><idno type="eISSN">1873-1597</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>A photosensitizer is a light-activated molecule that can generate reactive oxygen species or directly interact with nucleic acids. Both consequences can be applied to reduction of pathogens in various media and to selectively attack tumor cells. Numerous natural and synthesized photosensitizers have been identified for pathogen reduction.</p></div><div type="abstract" xml:lang="en"><p><b>METHODS</b></p><p>The photosensitizers of vitamins K3 (VK3), B1 (VB1), B6 (VB6) and benzophenone (BP) were prepared in 100-200 μM of PBS solution, irradiated with UVA at 0-48 J/cm</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS AND CONCLUSIONS</b></p><p>Over six logs CFU/ml reduction of E. coli suspended in PBS occurred after treatment with either VB1, VB6, VK3 or BP combined with UVA irradiation. When bacteria were suspended in plasma, two to seven logs reduction occurred depending on the UVA dose, photosensitizer concentration, and bacteria species. Among these photosensitizers, BP had the most potent bactericidal effect and is a promising UVA photosensitizer for pathogen reduction. The level of absorption spectrum alteration after UVA irradiation was profound for VK3 and VB6 but minimal for BP and VB1. The UV-vis absorption spectrum changes did not correlate with the bactericidal effect indicating that molecule modification by UVA light is not required for the bactericidal activity.</p></div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32165334</PMID><DateRevised><Year>2020</Year><Month>06</Month><Day>17</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-1597</ISSN><JournalIssue CitedMedium="Internet"><Volume>30</Volume><PubDate><Year>2020</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Photodiagnosis and photodynamic therapy</Title><ISOAbbreviation>Photodiagnosis Photodyn Ther</ISOAbbreviation></Journal><ArticleTitle>Inactivation of bacteria in plasma by photosensitizers benzophenone and vitamins K3, B1 and B6 with UV A light irradiation.</ArticleTitle><Pagination><MedlinePgn>101713</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S1572-1000(20)30066-1</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.pdpdt.2020.101713</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">A photosensitizer is a light-activated molecule that can generate reactive oxygen species or directly interact with nucleic acids. Both consequences can be applied to reduction of pathogens in various media and to selectively attack tumor cells. Numerous natural and synthesized photosensitizers have been identified for pathogen reduction.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">The photosensitizers of vitamins K3 (VK3), B1 (VB1), B6 (VB6) and benzophenone (BP) were prepared in 100-200 μM of PBS solution, irradiated with UVA at 0-48 J/cm<sup>2</sup> for absorption spectrum alterations analysis. Bacteria species of E. coli, B. cereus, S. aureus and K. pneumoniae were mixed with 0-200 mM concentration of compounds and exposed to UVA irradiation of different dose at 6, 12 or 18 J/cm<sup>2</sup> to assess the bactericidal effects.</AbstractText><AbstractText Label="RESULTS AND CONCLUSIONS" NlmCategory="CONCLUSIONS">Over six logs CFU/ml reduction of E. coli suspended in PBS occurred after treatment with either VB1, VB6, VK3 or BP combined with UVA irradiation. When bacteria were suspended in plasma, two to seven logs reduction occurred depending on the UVA dose, photosensitizer concentration, and bacteria species. Among these photosensitizers, BP had the most potent bactericidal effect and is a promising UVA photosensitizer for pathogen reduction. The level of absorption spectrum alteration after UVA irradiation was profound for VK3 and VB6 but minimal for BP and VB1. The UV-vis absorption spectrum changes did not correlate with the bactericidal effect indicating that molecule modification by UVA light is not required for the bactericidal activity.</AbstractText><CopyrightInformation>Copyright © 2020. Published by Elsevier B.V.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Fei</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, Center for Biologics Evaluation and Research, FDA, Silver Spring, MD, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Feyissa</LastName><ForeName>Qinati</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, Center for Biologics Evaluation and Research, FDA, Silver Spring, MD, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ibrahim</LastName><ForeName>Zina</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, Center for Biologics Evaluation and Research, FDA, Silver Spring, MD, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Ying</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, Center for Biologics Evaluation and Research, FDA, Silver Spring, MD, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Kevin L</ForeName><Initials>KL</Initials><AffiliationInfo><Affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, Center for Biologics Evaluation and Research, FDA, Silver Spring, MD, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guo</LastName><ForeName>Zihan</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, Center for Biologics Evaluation and Research, FDA, Silver Spring, MD, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ahmad</LastName><ForeName>Justen</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, Center for Biologics Evaluation and Research, FDA, Silver Spring, MD, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vostal</LastName><ForeName>Jaroslav G</ForeName><Initials>JG</Initials><AffiliationInfo><Affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, Center for Biologics Evaluation and Research, FDA, Silver Spring, MD, USA. Electronic address: Jaroslav.Vostal@fda.hhs.gov.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>03</Month><Day>09</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Photodiagnosis Photodyn Ther</MedlineTA><NlmUniqueID>101226123</NlmUniqueID><ISSNLinking>1572-1000</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Antimicrobial Photodynamic therapy</Keyword><Keyword MajorTopicYN="N">Pathogen reduction</Keyword><Keyword MajorTopicYN="N">Photosensitizer</Keyword><Keyword MajorTopicYN="N">Plasma</Keyword><Keyword MajorTopicYN="N">UV</Keyword><Keyword MajorTopicYN="N">Vitamin</Keyword></KeywordList><CoiStatement>Declaration of Competing Interest None.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>02</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>03</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>3</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>3</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>3</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32165334</ArticleId><ArticleId IdType="pii">S1572-1000(20)30066-1</ArticleId><ArticleId IdType="doi">10.1016/j.pdpdt.2020.101713</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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