Evaluation of behavior, growth, and swarming formation of Escherichia coli and Staphylococcus aureus in culture medium modified with silver nanoparticles.
Identifieur interne : 000072 ( Main/Corpus ); précédent : 000071; suivant : 000073Evaluation of behavior, growth, and swarming formation of Escherichia coli and Staphylococcus aureus in culture medium modified with silver nanoparticles.
Auteurs : Lucio Assis Araujo Neto ; Tatiane Melo Pereira ; Luciano Paulino SilvaSource :
- Microbial pathogenesis [ 1096-1208 ] ; 2020.
Abstract
Silver nanoparticles (AgNPs), as well as silver ions, are described as toxic to a broad spectrum of microorganisms, especially bacteria. In contrast to this, a current trend is to develop and carry out the in vitro cultivation of microorganisms, facilitating the study of interactions between populations of cells and species. Thus, the goal of this study was to evaluate the behavior, growth, and swarming formation of bacteria under conditions of co-culture in solid medium modified with AgNPs. The aqueous extract from the leaves of Handroanthus serratifolius was used to chemically reduce silver nitrate (AgNO3) solution, forming AgNPs. This synthesis route was performed in an aqueous medium at 50 °C for 3 h. The hydrodynamic diameter (HD) and polydispersity index (PdI) were obtained by dynamic light scattering (DLS), and Zeta potential (ZP) of the AgNPs were measured by electrophoretic mobility. Atomic force microscopy (AFM) was used to evaluate the shape of the AgNPs. Luria Bertani (LB) medium was used for the liquid culture steps and for the solid medium, bacterial agar was added. Solutions containing AgNPs or AgNO3 were added at final concentrations of 256, 128, or 64 μM. Subsequently, microorganism Escherichia coli ATCC® 8739 and Staphylococcus aureus ATCC® 25923 were plated with AgNPs, AgNO3, and control media. Analyses of the AgNPs showed an average HD of 76.02 ± 3.08 nm, PdI of 0.461 ± 0.012, and ZP of -21.5 ± 2.2 mV; in addition, AgNPs were nearly spherical. The solid culture medium elaborated and modified with AgNPs at the concentrations of 256 and 128 μM inhibited the growth of the tested microorganisms and decreased the swarming formation. However, those media modified at a concentration of 64 μM did not induce any alteration in the growth and proliferation of the microorganisms. Furthermore, it was observed that plates containing modified culture media with 128 μM, increased proximity between both co-cultured bacteria occurred. Thus, the application of AgNPs in solid culture media becomes a promising and potentially reproducible strategy for evaluating the behavior, swarming formation, and toxicity of AgNPs, making the understanding of possible bactericidal or bacteriostatic effects, and also colonizing strategies.
DOI: 10.1016/j.micpath.2020.104480
PubMed: 32926998
Links to Exploration step
pubmed:32926998Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Evaluation of behavior, growth, and swarming formation of Escherichia coli and Staphylococcus aureus in culture medium modified with silver nanoparticles.</title><author><name sortKey="Araujo Neto, Lucio Assis" sort="Araujo Neto, Lucio Assis" uniqKey="Araujo Neto L" first="Lucio Assis" last="Araujo Neto">Lucio Assis Araujo Neto</name><affiliation><nlm:affiliation>Embrapa Genetic Resources and Biotechnology, Laboratory of Nanobiotechnology (LNANO), Brasilia, 70770-917, DF, Brazil; Federal University of Parana (UFPR), Postgraduate Program in Pharmaceutical Sciences, Curitiba, 80210-170, PR, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Pereira, Tatiane Melo" sort="Pereira, Tatiane Melo" uniqKey="Pereira T" first="Tatiane Melo" last="Pereira">Tatiane Melo Pereira</name><affiliation><nlm:affiliation>Federal University of Parana (UFPR), Postgraduate Program in Pharmaceutical Sciences, Curitiba, 80210-170, PR, Brazil; University of Brasilia (UnB), Postgraduate Program in Nanoscience and Nanobiotechnology, Brasilia, 70910-900, DF, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Silva, Luciano Paulino" sort="Silva, Luciano Paulino" uniqKey="Silva L" first="Luciano Paulino" last="Silva">Luciano Paulino Silva</name><affiliation><nlm:affiliation>Embrapa Genetic Resources and Biotechnology, Laboratory of Nanobiotechnology (LNANO), Brasilia, 70770-917, DF, Brazil; Federal University of Parana (UFPR), Postgraduate Program in Pharmaceutical Sciences, Curitiba, 80210-170, PR, Brazil; University of Brasilia (UnB), Postgraduate Program in Nanoscience and Nanobiotechnology, Brasilia, 70910-900, DF, Brazil. Electronic address: luciano.paulino@embrapa.br.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32926998</idno><idno type="pmid">32926998</idno><idno type="doi">10.1016/j.micpath.2020.104480</idno><idno type="wicri:Area/Main/Corpus">000072</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000072</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Evaluation of behavior, growth, and swarming formation of Escherichia coli and Staphylococcus aureus in culture medium modified with silver nanoparticles.</title><author><name sortKey="Araujo Neto, Lucio Assis" sort="Araujo Neto, Lucio Assis" uniqKey="Araujo Neto L" first="Lucio Assis" last="Araujo Neto">Lucio Assis Araujo Neto</name><affiliation><nlm:affiliation>Embrapa Genetic Resources and Biotechnology, Laboratory of Nanobiotechnology (LNANO), Brasilia, 70770-917, DF, Brazil; Federal University of Parana (UFPR), Postgraduate Program in Pharmaceutical Sciences, Curitiba, 80210-170, PR, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Pereira, Tatiane Melo" sort="Pereira, Tatiane Melo" uniqKey="Pereira T" first="Tatiane Melo" last="Pereira">Tatiane Melo Pereira</name><affiliation><nlm:affiliation>Federal University of Parana (UFPR), Postgraduate Program in Pharmaceutical Sciences, Curitiba, 80210-170, PR, Brazil; University of Brasilia (UnB), Postgraduate Program in Nanoscience and Nanobiotechnology, Brasilia, 70910-900, DF, Brazil.</nlm:affiliation></affiliation></author><author><name sortKey="Silva, Luciano Paulino" sort="Silva, Luciano Paulino" uniqKey="Silva L" first="Luciano Paulino" last="Silva">Luciano Paulino Silva</name><affiliation><nlm:affiliation>Embrapa Genetic Resources and Biotechnology, Laboratory of Nanobiotechnology (LNANO), Brasilia, 70770-917, DF, Brazil; Federal University of Parana (UFPR), Postgraduate Program in Pharmaceutical Sciences, Curitiba, 80210-170, PR, Brazil; University of Brasilia (UnB), Postgraduate Program in Nanoscience and Nanobiotechnology, Brasilia, 70910-900, DF, Brazil. Electronic address: luciano.paulino@embrapa.br.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Microbial pathogenesis</title><idno type="eISSN">1096-1208</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Silver nanoparticles (AgNPs), as well as silver ions, are described as toxic to a broad spectrum of microorganisms, especially bacteria. In contrast to this, a current trend is to develop and carry out the in vitro cultivation of microorganisms, facilitating the study of interactions between populations of cells and species. Thus, the goal of this study was to evaluate the behavior, growth, and swarming formation of bacteria under conditions of co-culture in solid medium modified with AgNPs. The aqueous extract from the leaves of Handroanthus serratifolius was used to chemically reduce silver nitrate (AgNO<sub>3</sub>) solution, forming AgNPs. This synthesis route was performed in an aqueous medium at 50 °C for 3 h. The hydrodynamic diameter (HD) and polydispersity index (PdI) were obtained by dynamic light scattering (DLS), and Zeta potential (ZP) of the AgNPs were measured by electrophoretic mobility. Atomic force microscopy (AFM) was used to evaluate the shape of the AgNPs. Luria Bertani (LB) medium was used for the liquid culture steps and for the solid medium, bacterial agar was added. Solutions containing AgNPs or AgNO<sub>3</sub> were added at final concentrations of 256, 128, or 64 μM. Subsequently, microorganism Escherichia coli ATCC® 8739 and Staphylococcus aureus ATCC® 25923 were plated with AgNPs, AgNO<sub>3</sub>, and control media. Analyses of the AgNPs showed an average HD of 76.02 ± 3.08 nm, PdI of 0.461 ± 0.012, and ZP of -21.5 ± 2.2 mV; in addition, AgNPs were nearly spherical. The solid culture medium elaborated and modified with AgNPs at the concentrations of 256 and 128 μM inhibited the growth of the tested microorganisms and decreased the swarming formation. However, those media modified at a concentration of 64 μM did not induce any alteration in the growth and proliferation of the microorganisms. Furthermore, it was observed that plates containing modified culture media with 128 μM, increased proximity between both co-cultured bacteria occurred. Thus, the application of AgNPs in solid culture media becomes a promising and potentially reproducible strategy for evaluating the behavior, swarming formation, and toxicity of AgNPs, making the understanding of possible bactericidal or bacteriostatic effects, and also colonizing strategies.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32926998</PMID><DateRevised><Year>2020</Year><Month>12</Month><Day>11</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1096-1208</ISSN><JournalIssue CitedMedium="Internet"><Volume>149</Volume><PubDate><Year>2020</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Microbial pathogenesis</Title><ISOAbbreviation>Microb Pathog</ISOAbbreviation></Journal><ArticleTitle>Evaluation of behavior, growth, and swarming formation of Escherichia coli and Staphylococcus aureus in culture medium modified with silver nanoparticles.</ArticleTitle><Pagination><MedlinePgn>104480</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0882-4010(20)30846-9</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.micpath.2020.104480</ELocationID><Abstract><AbstractText>Silver nanoparticles (AgNPs), as well as silver ions, are described as toxic to a broad spectrum of microorganisms, especially bacteria. In contrast to this, a current trend is to develop and carry out the in vitro cultivation of microorganisms, facilitating the study of interactions between populations of cells and species. Thus, the goal of this study was to evaluate the behavior, growth, and swarming formation of bacteria under conditions of co-culture in solid medium modified with AgNPs. The aqueous extract from the leaves of Handroanthus serratifolius was used to chemically reduce silver nitrate (AgNO<sub>3</sub>) solution, forming AgNPs. This synthesis route was performed in an aqueous medium at 50 °C for 3 h. The hydrodynamic diameter (HD) and polydispersity index (PdI) were obtained by dynamic light scattering (DLS), and Zeta potential (ZP) of the AgNPs were measured by electrophoretic mobility. Atomic force microscopy (AFM) was used to evaluate the shape of the AgNPs. Luria Bertani (LB) medium was used for the liquid culture steps and for the solid medium, bacterial agar was added. Solutions containing AgNPs or AgNO<sub>3</sub> were added at final concentrations of 256, 128, or 64 μM. Subsequently, microorganism Escherichia coli ATCC® 8739 and Staphylococcus aureus ATCC® 25923 were plated with AgNPs, AgNO<sub>3</sub>, and control media. Analyses of the AgNPs showed an average HD of 76.02 ± 3.08 nm, PdI of 0.461 ± 0.012, and ZP of -21.5 ± 2.2 mV; in addition, AgNPs were nearly spherical. The solid culture medium elaborated and modified with AgNPs at the concentrations of 256 and 128 μM inhibited the growth of the tested microorganisms and decreased the swarming formation. However, those media modified at a concentration of 64 μM did not induce any alteration in the growth and proliferation of the microorganisms. Furthermore, it was observed that plates containing modified culture media with 128 μM, increased proximity between both co-cultured bacteria occurred. Thus, the application of AgNPs in solid culture media becomes a promising and potentially reproducible strategy for evaluating the behavior, swarming formation, and toxicity of AgNPs, making the understanding of possible bactericidal or bacteriostatic effects, and also colonizing strategies.</AbstractText><CopyrightInformation>Copyright © 2020 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Araujo Neto</LastName><ForeName>Lucio Assis</ForeName><Initials>LA</Initials><AffiliationInfo><Affiliation>Embrapa Genetic Resources and Biotechnology, Laboratory of Nanobiotechnology (LNANO), Brasilia, 70770-917, DF, Brazil; Federal University of Parana (UFPR), Postgraduate Program in Pharmaceutical Sciences, Curitiba, 80210-170, PR, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pereira</LastName><ForeName>Tatiane Melo</ForeName><Initials>TM</Initials><AffiliationInfo><Affiliation>Federal University of Parana (UFPR), Postgraduate Program in Pharmaceutical Sciences, Curitiba, 80210-170, PR, Brazil; University of Brasilia (UnB), Postgraduate Program in Nanoscience and Nanobiotechnology, Brasilia, 70910-900, DF, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Silva</LastName><ForeName>Luciano Paulino</ForeName><Initials>LP</Initials><AffiliationInfo><Affiliation>Embrapa Genetic Resources and Biotechnology, Laboratory of Nanobiotechnology (LNANO), Brasilia, 70770-917, DF, Brazil; Federal University of Parana (UFPR), Postgraduate Program in Pharmaceutical Sciences, Curitiba, 80210-170, PR, Brazil; University of Brasilia (UnB), Postgraduate Program in Nanoscience and Nanobiotechnology, Brasilia, 70910-900, DF, Brazil. Electronic address: luciano.paulino@embrapa.br.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>09</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Microb Pathog</MedlineTA><NlmUniqueID>8606191</NlmUniqueID><ISSNLinking>0882-4010</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Green synthesis</Keyword><Keyword MajorTopicYN="N">Modified culture medium</Keyword><Keyword MajorTopicYN="N">Silver nanoparticles</Keyword><Keyword MajorTopicYN="N">Swarming</Keyword><Keyword MajorTopicYN="N">Toxicity</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>09</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>02</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>08</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>9</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>9</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>9</Month><Day>14</Day><Hour>20</Hour><Minute>12</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32926998</ArticleId><ArticleId IdType="pii">S0882-4010(20)30846-9</ArticleId><ArticleId IdType="doi">10.1016/j.micpath.2020.104480</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Terre/explor/SilverBacteriV1/Data/Main/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000072 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 000072 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Terre
|area= SilverBacteriV1
|flux= Main
|étape= Corpus
|type= RBID
|clé= pubmed:32926998
|texte= Evaluation of behavior, growth, and swarming formation of Escherichia coli and Staphylococcus aureus in culture medium modified with silver nanoparticles.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Corpus/RBID.i -Sk "pubmed:32926998" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a SilverBacteriV1
| This area was generated with Dilib version V0.6.38. |  |