Synergistic bactericidal effects of pairs of photosensitizer molecules activated by ultraviolet A light against bacteria in plasma.
Identifieur interne : 000033 ( Main/Corpus ); précédent : 000032; suivant : 000034Synergistic bactericidal effects of pairs of photosensitizer molecules activated by ultraviolet A light against bacteria in plasma.
Auteurs : Qinati Feyissa ; Fei Xu ; Zina Ibrahim ; Ying Li ; Kevin L. Xu ; Zihan Guo ; Justen Ahmad ; Jaroslav G. VostalSource :
- Transfusion [ 1537-2995 ] ; 2020.
Abstract
BACKGROUND
The current approach to reducing bacterial contamination in blood transfusion products is through detection or pathogen reduction methods, some of which utilize ultraviolet (UV) light photosensitizers. A small number of photosensitizers are being used as single agents in combination with UV light, but their efficacy can be limited against some pathogens. Benzophenone (BP) and vitamins B1, B6, and K3 have been identified as effective UVA photosensitizers for inactivation of bacteria. We evaluated whether combining pairs of photosensitizers in this group would have synergistic bactericidal effects on Gram-negative and Gram-positive bacteria.
STUDY DESIGN AND METHODS
Bacteria species of Escherichia coli, Bacillus cereus, Staphylococcus aureus, and Klebsiella pneumoniae were mixed with 0 to 100 mM concentrations of photosensitizers and exposed to UVA irradiation at 18 J/cm
RESULTS
Single photosensitizers irradiated with UVA produced a range of bactericidal activity. When combined in pairs, all demonstrated some synergistic bactericidal effects with up to 4-log reduction above the sum of activities of individual molecules in the pair against bacteria in plasma. Photosensitizer pairs with BP had the highest synergism across all bacteria. With vitamin K3 in the pair, synergism was evident for Gram-positive but not for Gram-negative bacteria. Vitamin B1 and vitamin B6 had the least synergism. These results indicate that a combination approach with multiple photosensitizers may extend effectiveness of pathogen reduction in plasma.
CONCLUSIONS
Combining photosensitizers in pathogen reduction methods could improve bactericidal efficacy and lead to use of lower concentrations of photosensitizers to reduce toxicities and unwanted side effects.
DOI: 10.1111/trf.16180
PubMed: 33219568
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Synergistic bactericidal effects of pairs of photosensitizer molecules activated by ultraviolet A light against bacteria in plasma.</title><author><name sortKey="Feyissa, Qinati" sort="Feyissa, Qinati" uniqKey="Feyissa Q" first="Qinati" last="Feyissa">Qinati Feyissa</name><affiliation><nlm:affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, CBER, FDA, Silver Spring, Maryland.</nlm:affiliation></affiliation></author><author><name sortKey="Xu, Fei" sort="Xu, Fei" uniqKey="Xu F" first="Fei" last="Xu">Fei Xu</name><affiliation><nlm:affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, CBER, FDA, Silver Spring, Maryland.</nlm:affiliation></affiliation></author><author><name sortKey="Ibrahim, Zina" sort="Ibrahim, Zina" uniqKey="Ibrahim Z" first="Zina" last="Ibrahim">Zina Ibrahim</name><affiliation><nlm:affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, CBER, FDA, Silver Spring, Maryland.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Ying" sort="Li, Ying" uniqKey="Li Y" first="Ying" last="Li">Ying Li</name><affiliation><nlm:affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, CBER, FDA, Silver Spring, Maryland.</nlm:affiliation></affiliation></author><author><name sortKey="Xu, Kevin L" sort="Xu, Kevin L" uniqKey="Xu K" first="Kevin L" last="Xu">Kevin L. Xu</name><affiliation><nlm:affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, CBER, FDA, Silver Spring, Maryland.</nlm:affiliation></affiliation></author><author><name sortKey="Guo, Zihan" sort="Guo, Zihan" uniqKey="Guo Z" first="Zihan" last="Guo">Zihan Guo</name><affiliation><nlm:affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, CBER, FDA, Silver Spring, Maryland.</nlm:affiliation></affiliation></author><author><name sortKey="Ahmad, Justen" sort="Ahmad, Justen" uniqKey="Ahmad J" first="Justen" last="Ahmad">Justen Ahmad</name><affiliation><nlm:affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, CBER, FDA, Silver Spring, Maryland.</nlm:affiliation></affiliation></author><author><name sortKey="Vostal, Jaroslav G" sort="Vostal, Jaroslav G" uniqKey="Vostal J" first="Jaroslav G" last="Vostal">Jaroslav G. Vostal</name><affiliation><nlm:affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, CBER, FDA, Silver Spring, Maryland.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:33219568</idno><idno type="pmid">33219568</idno><idno type="doi">10.1111/trf.16180</idno><idno type="wicri:Area/Main/Corpus">000033</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000033</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Synergistic bactericidal effects of pairs of photosensitizer molecules activated by ultraviolet A light against bacteria in plasma.</title><author><name sortKey="Feyissa, Qinati" sort="Feyissa, Qinati" uniqKey="Feyissa Q" first="Qinati" last="Feyissa">Qinati Feyissa</name><affiliation><nlm:affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, CBER, FDA, Silver Spring, Maryland.</nlm:affiliation></affiliation></author><author><name sortKey="Xu, Fei" sort="Xu, Fei" uniqKey="Xu F" first="Fei" last="Xu">Fei Xu</name><affiliation><nlm:affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, CBER, FDA, Silver Spring, Maryland.</nlm:affiliation></affiliation></author><author><name sortKey="Ibrahim, Zina" sort="Ibrahim, Zina" uniqKey="Ibrahim Z" first="Zina" last="Ibrahim">Zina Ibrahim</name><affiliation><nlm:affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, CBER, FDA, Silver Spring, Maryland.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Ying" sort="Li, Ying" uniqKey="Li Y" first="Ying" last="Li">Ying Li</name><affiliation><nlm:affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, CBER, FDA, Silver Spring, Maryland.</nlm:affiliation></affiliation></author><author><name sortKey="Xu, Kevin L" sort="Xu, Kevin L" uniqKey="Xu K" first="Kevin L" last="Xu">Kevin L. Xu</name><affiliation><nlm:affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, CBER, FDA, Silver Spring, Maryland.</nlm:affiliation></affiliation></author><author><name sortKey="Guo, Zihan" sort="Guo, Zihan" uniqKey="Guo Z" first="Zihan" last="Guo">Zihan Guo</name><affiliation><nlm:affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, CBER, FDA, Silver Spring, Maryland.</nlm:affiliation></affiliation></author><author><name sortKey="Ahmad, Justen" sort="Ahmad, Justen" uniqKey="Ahmad J" first="Justen" last="Ahmad">Justen Ahmad</name><affiliation><nlm:affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, CBER, FDA, Silver Spring, Maryland.</nlm:affiliation></affiliation></author><author><name sortKey="Vostal, Jaroslav G" sort="Vostal, Jaroslav G" uniqKey="Vostal J" first="Jaroslav G" last="Vostal">Jaroslav G. Vostal</name><affiliation><nlm:affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, CBER, FDA, Silver Spring, Maryland.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Transfusion</title><idno type="eISSN">1537-2995</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>The current approach to reducing bacterial contamination in blood transfusion products is through detection or pathogen reduction methods, some of which utilize ultraviolet (UV) light photosensitizers. A small number of photosensitizers are being used as single agents in combination with UV light, but their efficacy can be limited against some pathogens. Benzophenone (BP) and vitamins B1, B6, and K3 have been identified as effective UVA photosensitizers for inactivation of bacteria. We evaluated whether combining pairs of photosensitizers in this group would have synergistic bactericidal effects on Gram-negative and Gram-positive bacteria.</p></div><div type="abstract" xml:lang="en"><p><b>STUDY DESIGN AND METHODS</b></p><p>Bacteria species of Escherichia coli, Bacillus cereus, Staphylococcus aureus, and Klebsiella pneumoniae were mixed with 0 to 100 mM concentrations of photosensitizers and exposed to UVA irradiation at 18 J/cm</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>Single photosensitizers irradiated with UVA produced a range of bactericidal activity. When combined in pairs, all demonstrated some synergistic bactericidal effects with up to 4-log reduction above the sum of activities of individual molecules in the pair against bacteria in plasma. Photosensitizer pairs with BP had the highest synergism across all bacteria. With vitamin K3 in the pair, synergism was evident for Gram-positive but not for Gram-negative bacteria. Vitamin B1 and vitamin B6 had the least synergism. These results indicate that a combination approach with multiple photosensitizers may extend effectiveness of pathogen reduction in plasma.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>Combining photosensitizers in pathogen reduction methods could improve bactericidal efficacy and lead to use of lower concentrations of photosensitizers to reduce toxicities and unwanted side effects.</p></div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">33219568</PMID><DateRevised><Year>2020</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1537-2995</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2020</Year><Month>Nov</Month><Day>21</Day></PubDate></JournalIssue><Title>Transfusion</Title><ISOAbbreviation>Transfusion</ISOAbbreviation></Journal><ArticleTitle>Synergistic bactericidal effects of pairs of photosensitizer molecules activated by ultraviolet A light against bacteria in plasma.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.1111/trf.16180</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">The current approach to reducing bacterial contamination in blood transfusion products is through detection or pathogen reduction methods, some of which utilize ultraviolet (UV) light photosensitizers. A small number of photosensitizers are being used as single agents in combination with UV light, but their efficacy can be limited against some pathogens. Benzophenone (BP) and vitamins B1, B6, and K3 have been identified as effective UVA photosensitizers for inactivation of bacteria. We evaluated whether combining pairs of photosensitizers in this group would have synergistic bactericidal effects on Gram-negative and Gram-positive bacteria.</AbstractText><AbstractText Label="STUDY DESIGN AND METHODS" NlmCategory="METHODS">Bacteria species of Escherichia coli, Bacillus cereus, Staphylococcus aureus, and Klebsiella pneumoniae were mixed with 0 to 100 mM concentrations of photosensitizers and exposed to UVA irradiation at 18 J/cm<sup>2</sup> to assess their bactericidal effects.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Single photosensitizers irradiated with UVA produced a range of bactericidal activity. When combined in pairs, all demonstrated some synergistic bactericidal effects with up to 4-log reduction above the sum of activities of individual molecules in the pair against bacteria in plasma. Photosensitizer pairs with BP had the highest synergism across all bacteria. With vitamin K3 in the pair, synergism was evident for Gram-positive but not for Gram-negative bacteria. Vitamin B1 and vitamin B6 had the least synergism. These results indicate that a combination approach with multiple photosensitizers may extend effectiveness of pathogen reduction in plasma.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">Combining photosensitizers in pathogen reduction methods could improve bactericidal efficacy and lead to use of lower concentrations of photosensitizers to reduce toxicities and unwanted side effects.</AbstractText><CopyrightInformation>© 2020 AABB.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Feyissa</LastName><ForeName>Qinati</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, CBER, FDA, Silver Spring, Maryland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Fei</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, CBER, FDA, Silver Spring, Maryland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ibrahim</LastName><ForeName>Zina</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, CBER, FDA, Silver Spring, Maryland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Ying</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, CBER, FDA, Silver Spring, Maryland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Kevin L</ForeName><Initials>KL</Initials><AffiliationInfo><Affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, CBER, FDA, Silver Spring, Maryland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guo</LastName><ForeName>Zihan</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, CBER, FDA, Silver Spring, Maryland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ahmad</LastName><ForeName>Justen</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, CBER, FDA, Silver Spring, Maryland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vostal</LastName><ForeName>Jaroslav G</ForeName><Initials>JG</Initials><AffiliationInfo><Affiliation>Laboratory of Cellular Hematology, Division of Blood Components and Devices, CBER, FDA, Silver Spring, Maryland.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>11</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Transfusion</MedlineTA><NlmUniqueID>0417360</NlmUniqueID><ISSNLinking>0041-1132</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">donors</Keyword><Keyword MajorTopicYN="N">hematology - platelets</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>08</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>10</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>10</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>11</Month><Day>21</Day><Hour>5</Hour><Minute>38</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>11</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>11</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33219568</ArticleId><ArticleId IdType="doi">10.1111/trf.16180</ArticleId></ArticleIdList><ReferenceList><Title>REFERENCES</Title><Reference><Citation>Perelson AS, Essunger P, Cao Y, et al. 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