Stress response of heavy metal mixture present in wastewater and leachate on heat-shock protein 47-transfected cells.
Identifieur interne : 000100 ( PubMed/Curation ); précédent : 000099; suivant : 000101Stress response of heavy metal mixture present in wastewater and leachate on heat-shock protein 47-transfected cells.
Auteurs : Fahmi Ben Fredj [Japon] ; Mitsuteru Irie ; Junkyu Han ; Parida Yamada ; Atef Limam ; Ahmed Ghrabi ; Takahiro Morio ; Hiroko IsodaSource :
- Environmental toxicology and chemistry [ 1552-8618 ] ; 2010.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : HSP47 Heat-Shock Proteins.
- chemical , toxicity : Metals, Heavy, Water Pollutants, Chemical.
- Animals, CHO Cells, Cricetinae, Cricetulus, Stress, Physiological, Transfection.
Abstract
Heavy metals present in water environment and hazardous sites as single compounds or mixture may drastically affect human health. In the present work, we investigated the risk assessment of wastewater effluents and leachate with a focus on three heavy metals-nickel (Ni), cadmium (Cd), and lead (Pb)-and their combined effect on mammalian cells, using Chinese hamster ovary cells transfected with the heat-shock protein (HSP) 47 promoter. The heavy metal mixture model was designed based on the concentrations of metals in wastewater effluents and leachate sampled in Tunisia. Using a ternary diagram, we investigated the stress response of the interaction model. This research indicated that the single heavy metals induced the stress response on HSP(+) cells even at concentrations lower than the local and international guidelines. Differences in water quality likely influenced the metal responses such that the organic composition of the leachate increased the stress response induced by the heavy metals exclusively, whereas the effluents included organic compounds that were able to mask the heavy metal effect. The mixture characterization discovered the key role played by the high levels of Ni or combination of Cd and Pb to induce the highest stress response following 3-h incubation. Heat-shock protein 47 has proven its effectiveness for assessing the heavy metal mixture effect even at low concentrations. Furthermore, the combination of a bioassay system with a statistical model proved extremely useful for better understanding the major contributors to the stress response of the mixture.
DOI: 10.1002/etc.178
PubMed: 20821615
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Han</name></author><author><name sortKey="Yamada, Parida" sort="Yamada, Parida" uniqKey="Yamada P" first="Parida" last="Yamada">Parida Yamada</name></author><author><name sortKey="Limam, Atef" sort="Limam, Atef" uniqKey="Limam A" first="Atef" last="Limam">Atef Limam</name></author><author><name sortKey="Ghrabi, Ahmed" sort="Ghrabi, Ahmed" uniqKey="Ghrabi A" first="Ahmed" last="Ghrabi">Ahmed Ghrabi</name></author><author><name sortKey="Morio, Takahiro" sort="Morio, Takahiro" uniqKey="Morio T" first="Takahiro" last="Morio">Takahiro Morio</name></author><author><name sortKey="Isoda, Hiroko" sort="Isoda, Hiroko" uniqKey="Isoda H" first="Hiroko" last="Isoda">Hiroko Isoda</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:20821615</idno><idno type="pmid">20821615</idno><idno type="doi">10.1002/etc.178</idno><idno type="wicri:Area/PubMed/Corpus">000100</idno><idno type="wicri:explorRef" wicri:stream="PubMed" 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Physiological</term><term>Transfection</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Heavy metals present in water environment and hazardous sites as single compounds or mixture may drastically affect human health. In the present work, we investigated the risk assessment of wastewater effluents and leachate with a focus on three heavy metals-nickel (Ni), cadmium (Cd), and lead (Pb)-and their combined effect on mammalian cells, using Chinese hamster ovary cells transfected with the heat-shock protein (HSP) 47 promoter. The heavy metal mixture model was designed based on the concentrations of metals in wastewater effluents and leachate sampled in Tunisia. Using a ternary diagram, we investigated the stress response of the interaction model. This research indicated that the single heavy metals induced the stress response on HSP(+) cells even at concentrations lower than the local and international guidelines. Differences in water quality likely influenced the metal responses such that the organic composition of the leachate increased the stress response induced by the heavy metals exclusively, whereas the effluents included organic compounds that were able to mask the heavy metal effect. The mixture characterization discovered the key role played by the high levels of Ni or combination of Cd and Pb to induce the highest stress response following 3-h incubation. Heat-shock protein 47 has proven its effectiveness for assessing the heavy metal mixture effect even at low concentrations. Furthermore, the combination of a bioassay system with a statistical model proved extremely useful for better understanding the major contributors to the stress response of the mixture.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20821615</PMID><DateCreated><Year>2010</Year><Month>09</Month><Day>07</Day></DateCreated><DateCompleted><Year>2010</Year><Month>09</Month><Day>27</Day></DateCompleted><DateRevised><Year>2016</Year><Month>10</Month><Day>18</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1552-8618</ISSN><JournalIssue CitedMedium="Internet"><Volume>29</Volume><Issue>7</Issue><PubDate><Year>2010</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Environmental toxicology and chemistry</Title><ISOAbbreviation>Environ. Toxicol. Chem.</ISOAbbreviation></Journal><ArticleTitle>Stress response of heavy metal mixture present in wastewater and leachate on heat-shock protein 47-transfected cells.</ArticleTitle><Pagination><MedlinePgn>1637-47</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/etc.178</ELocationID><Abstract><AbstractText>Heavy metals present in water environment and hazardous sites as single compounds or mixture may drastically affect human health. In the present work, we investigated the risk assessment of wastewater effluents and leachate with a focus on three heavy metals-nickel (Ni), cadmium (Cd), and lead (Pb)-and their combined effect on mammalian cells, using Chinese hamster ovary cells transfected with the heat-shock protein (HSP) 47 promoter. The heavy metal mixture model was designed based on the concentrations of metals in wastewater effluents and leachate sampled in Tunisia. Using a ternary diagram, we investigated the stress response of the interaction model. This research indicated that the single heavy metals induced the stress response on HSP(+) cells even at concentrations lower than the local and international guidelines. Differences in water quality likely influenced the metal responses such that the organic composition of the leachate increased the stress response induced by the heavy metals exclusively, whereas the effluents included organic compounds that were able to mask the heavy metal effect. The mixture characterization discovered the key role played by the high levels of Ni or combination of Cd and Pb to induce the highest stress response following 3-h incubation. Heat-shock protein 47 has proven its effectiveness for assessing the heavy metal mixture effect even at low concentrations. Furthermore, the combination of a bioassay system with a statistical model proved extremely useful for better understanding the major contributors to the stress response of the mixture.</AbstractText><CopyrightInformation>Copyright (c) 2010 SETAC.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ben Fredj</LastName><ForeName>Fahmi</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba City, Ibaraki 305-8572, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Irie</LastName><ForeName>Mitsuteru</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Han</LastName><ForeName>Junkyu</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Yamada</LastName><ForeName>Parida</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Limam</LastName><ForeName>Atef</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Ghrabi</LastName><ForeName>Ahmed</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Morio</LastName><ForeName>Takahiro</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Isoda</LastName><ForeName>Hiroko</ForeName><Initials>H</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Environ Toxicol Chem</MedlineTA><NlmUniqueID>8308958</NlmUniqueID><ISSNLinking>0730-7268</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D050958">HSP47 Heat-Shock Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019216">Metals, Heavy</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014874">Water Pollutants, Chemical</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016466" MajorTopicYN="N">CHO Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006224" MajorTopicYN="N">Cricetinae</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003412" MajorTopicYN="N">Cricetulus</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D050958" MajorTopicYN="N">HSP47 Heat-Shock Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019216" MajorTopicYN="N">Metals, Heavy</DescriptorName><QualifierName UI="Q000633" MajorTopicYN="Y">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013312" MajorTopicYN="Y">Stress, Physiological</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014162" MajorTopicYN="Y">Transfection</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014874" MajorTopicYN="N">Water Pollutants, Chemical</DescriptorName><QualifierName UI="Q000633" MajorTopicYN="Y">toxicity</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>9</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>9</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>9</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20821615</ArticleId><ArticleId IdType="doi">10.1002/etc.178</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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