NickelMaghrebV1, Pmc, Corpus, bibRecord, 000021

IncH-Type Plasmid Harboring blaCTX-M-15, blaDHA-1, and qnrB4 Genes Recovered from Animal Isolates

Identifieur interne : 000021 ( Pmc/Corpus ); précédent : 000020; suivant : 000022

IncH-Type Plasmid Harboring blaCTX-M-15, blaDHA-1, and qnrB4 Genes Recovered from Animal Isolates

Auteurs : Andreas Schlüter ; Patrice Nordmann ; Rémy A. Bonnin ; Yves Millemann ; Felix G. Eikmeyer ; Daniel Wibberg ; Alfred Pühler ; Laurent Poirel

Source :

Antimicrobial Agents and Chemotherapy [ 0066-4804 ] ; 2014.

RBID : PMC:4068538

Abstract

The whole sequence of plasmid pENVA carrying the extended-spectrum β-lactamase gene bla_CTX-M-15 was determined. It was identified from a series of clonally related Klebsiella pneumoniae sequence type 274 strains recovered from companion animals. This plasmid was 253,984 bp in size and harbored, in addition to bla_CTX-M-15, a large array of genes encoding resistance to many antibiotic molecules, including β-lactams (bla_TEM-1, bla_DHA-1), aminoglycosides (aacA2, aadA1), tetracycline (tetA), quinolones (qnrB4), trimethoprim (dfrA15), and sulfonamides (two copies of sul1). In addition, genes encoding resistance to mercury, tellurium, nickel, and quaternary compounds were identified. It also carried genes encoding DNA damage protection and mutagenesis repair and a locus for a CRISPR system, which corresponds to an immune system involved in protection against bacteriophages and plasmids. Comparative analysis of the plasmid scaffold showed that it possessed a structure similar to that of only a single plasmid, which was pNDM-MAR encoding the carbapenemase NDM-1 and identified from human K. pneumoniae isolates. Both plasmids possessed two replicons, namely, those of IncFIB-like and IncHIB-like plasmids, which were significantly different from those previously characterized. The bla_CTX-M-15 gene, together with the other antibiotic resistance genes, was part of a large module likely acquired through a transposition process. We characterized here a new plasmid type carrying the bla_CTX-M-15 gene identified in a K. pneumoniae isolate of animal origin. The extent to which this plasmid type may spread efficiently and possibly further enhance the dissemination of bla_CTX-M-15 among animal and human isolates remains to be determined.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4068538

DOI: 10.1128/AAC.02695-14
PubMed: 24752252
PubMed Central: 4068538

Links to Exploration step

PMC:4068538

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">IncH-Type Plasmid Harboring <italic>bla</italic>
<sub>CTX-M-15</sub>
, <italic>bla</italic>
<sub>DHA-1</sub>
, and <italic>qnrB4</italic>
 Genes Recovered from Animal Isolates</title>
<author><name sortKey="Schluter, Andreas" sort="Schluter, Andreas" uniqKey="Schluter A" first="Andreas" last="Schlüter">Andreas Schlüter</name>
<affiliation><nlm:aff id="aff1">Center for Biotechnology (CeBiTec), Institute for Genome Research and Systems Biology, Bielefeld University, Bielefeld, Germany</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Nordmann, Patrice" sort="Nordmann, Patrice" uniqKey="Nordmann P" first="Patrice" last="Nordmann">Patrice Nordmann</name>
<affiliation><nlm:aff id="aff2">INSERM U914, South-Paris Medical School, Le Kremlin-Bicêtre, France</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff3">Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Bonnin, Remy A" sort="Bonnin, Remy A" uniqKey="Bonnin R" first="Rémy A." last="Bonnin">Rémy A. Bonnin</name>
<affiliation><nlm:aff id="aff2">INSERM U914, South-Paris Medical School, Le Kremlin-Bicêtre, France</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Millemann, Yves" sort="Millemann, Yves" uniqKey="Millemann Y" first="Yves" last="Millemann">Yves Millemann</name>
<affiliation><nlm:aff id="aff4">Unité Microbiologie Alimentaire, Sécurité et Qualité des Aliments (MASQ), Ecole Nationale Vétérinaire d'Alfort, Université Paris-Est, Maisons-Alfort, France</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Eikmeyer, Felix G" sort="Eikmeyer, Felix G" uniqKey="Eikmeyer F" first="Felix G." last="Eikmeyer">Felix G. Eikmeyer</name>
<affiliation><nlm:aff id="aff1">Center for Biotechnology (CeBiTec), Institute for Genome Research and Systems Biology, Bielefeld University, Bielefeld, Germany</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wibberg, Daniel" sort="Wibberg, Daniel" uniqKey="Wibberg D" first="Daniel" last="Wibberg">Daniel Wibberg</name>
<affiliation><nlm:aff id="aff1">Center for Biotechnology (CeBiTec), Institute for Genome Research and Systems Biology, Bielefeld University, Bielefeld, Germany</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Puhler, Alfred" sort="Puhler, Alfred" uniqKey="Puhler A" first="Alfred" last="Pühler">Alfred Pühler</name>
<affiliation><nlm:aff id="aff1">Center for Biotechnology (CeBiTec), Institute for Genome Research and Systems Biology, Bielefeld University, Bielefeld, Germany</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Poirel, Laurent" sort="Poirel, Laurent" uniqKey="Poirel L" first="Laurent" last="Poirel">Laurent Poirel</name>
<affiliation><nlm:aff id="aff2">INSERM U914, South-Paris Medical School, Le Kremlin-Bicêtre, France</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff3">Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland</nlm:aff>
</affiliation>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">IncH-Type Plasmid Harboring <italic>bla</italic>
<sub>CTX-M-15</sub>
, <italic>bla</italic>
<sub>DHA-1</sub>
, and <italic>qnrB4</italic>
 Genes Recovered from Animal Isolates</title>
<author><name sortKey="Schluter, Andreas" sort="Schluter, Andreas" uniqKey="Schluter A" first="Andreas" last="Schlüter">Andreas Schlüter</name>
<affiliation><nlm:aff id="aff1">Center for Biotechnology (CeBiTec), Institute for Genome Research and Systems Biology, Bielefeld University, Bielefeld, Germany</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Nordmann, Patrice" sort="Nordmann, Patrice" uniqKey="Nordmann P" first="Patrice" last="Nordmann">Patrice Nordmann</name>
<affiliation><nlm:aff id="aff2">INSERM U914, South-Paris Medical School, Le Kremlin-Bicêtre, France</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff3">Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Bonnin, Remy A" sort="Bonnin, Remy A" uniqKey="Bonnin R" first="Rémy A." last="Bonnin">Rémy A. Bonnin</name>
<affiliation><nlm:aff id="aff2">INSERM U914, South-Paris Medical School, Le Kremlin-Bicêtre, France</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Millemann, Yves" sort="Millemann, Yves" uniqKey="Millemann Y" first="Yves" last="Millemann">Yves Millemann</name>
<affiliation><nlm:aff id="aff4">Unité Microbiologie Alimentaire, Sécurité et Qualité des Aliments (MASQ), Ecole Nationale Vétérinaire d'Alfort, Université Paris-Est, Maisons-Alfort, France</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Eikmeyer, Felix G" sort="Eikmeyer, Felix G" uniqKey="Eikmeyer F" first="Felix G." last="Eikmeyer">Felix G. Eikmeyer</name>
<affiliation><nlm:aff id="aff1">Center for Biotechnology (CeBiTec), Institute for Genome Research and Systems Biology, Bielefeld University, Bielefeld, Germany</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wibberg, Daniel" sort="Wibberg, Daniel" uniqKey="Wibberg D" first="Daniel" last="Wibberg">Daniel Wibberg</name>
<affiliation><nlm:aff id="aff1">Center for Biotechnology (CeBiTec), Institute for Genome Research and Systems Biology, Bielefeld University, Bielefeld, Germany</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Puhler, Alfred" sort="Puhler, Alfred" uniqKey="Puhler A" first="Alfred" last="Pühler">Alfred Pühler</name>
<affiliation><nlm:aff id="aff1">Center for Biotechnology (CeBiTec), Institute for Genome Research and Systems Biology, Bielefeld University, Bielefeld, Germany</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Poirel, Laurent" sort="Poirel, Laurent" uniqKey="Poirel L" first="Laurent" last="Poirel">Laurent Poirel</name>
<affiliation><nlm:aff id="aff2">INSERM U914, South-Paris Medical School, Le Kremlin-Bicêtre, France</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff3">Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland</nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">Antimicrobial Agents and Chemotherapy</title>
<idno type="ISSN">0066-4804</idno>
<idno type="eISSN">1098-6596</idno>
<imprint><date when="2014">2014</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
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<profileDesc><textClass></textClass>
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<front><div type="abstract" xml:lang="en"><p>The whole sequence of plasmid pENVA carrying the extended-spectrum β-lactamase gene <italic>bla</italic>
<sub>CTX-M-15</sub>
 was determined. It was identified from a series of clonally related <named-content content-type="genus-species">Klebsiella pneumoniae</named-content>
 sequence type 274 strains recovered from companion animals. This plasmid was 253,984 bp in size and harbored, in addition to <italic>bla</italic>
<sub>CTX-M-15</sub>
, a large array of genes encoding resistance to many antibiotic molecules, including β-lactams (<italic>bla</italic>
<sub>TEM-1</sub>
, <italic>bla</italic>
<sub>DHA-1</sub>
), aminoglycosides (<italic>aacA2</italic>
, <italic>aadA1</italic>
), tetracycline (<italic>tetA</italic>
), quinolones (<italic>qnrB4</italic>
), trimethoprim (<italic>dfrA15</italic>
), and sulfonamides (two copies of <italic>sul1</italic>
). In addition, genes encoding resistance to mercury, tellurium, nickel, and quaternary compounds were identified. It also carried genes encoding DNA damage protection and mutagenesis repair and a locus for a CRISPR system, which corresponds to an immune system involved in protection against bacteriophages and plasmids. Comparative analysis of the plasmid scaffold showed that it possessed a structure similar to that of only a single plasmid, which was pNDM-MAR encoding the carbapenemase NDM-1 and identified from human <named-content content-type="genus-species">K. pneumoniae</named-content>
 isolates. Both plasmids possessed two replicons, namely, those of IncFIB-like and IncHIB-like plasmids, which were significantly different from those previously characterized. The <italic>bla</italic>
<sub>CTX-M-15</sub>
 gene, together with the other antibiotic resistance genes, was part of a large module likely acquired through a transposition process. We characterized here a new plasmid type carrying the <italic>bla</italic>
<sub>CTX-M-15</sub>
 gene identified in a <named-content content-type="genus-species">K. pneumoniae</named-content>
 isolate of animal origin. The extent to which this plasmid type may spread efficiently and possibly further enhance the dissemination of <italic>bla</italic>
<sub>CTX-M-15</sub>
 among animal and human isolates remains to be determined.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
  <front><journal-meta><journal-id journal-id-type="nlm-ta">Antimicrob Agents Chemother</journal-id>
<journal-id journal-id-type="iso-abbrev">Antimicrob. Agents Chemother</journal-id>
<journal-id journal-id-type="hwp">aac</journal-id>
<journal-id journal-id-type="pmc">aac</journal-id>
<journal-id journal-id-type="publisher-id">AAC</journal-id>
<journal-title-group><journal-title>Antimicrobial Agents and Chemotherapy</journal-title>
</journal-title-group>
<issn pub-type="ppub">0066-4804</issn>
<issn pub-type="epub">1098-6596</issn>
<publisher><publisher-name>American Society for Microbiology</publisher-name>
<publisher-loc>1752 N St., N.W., Washington, DC</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">24752252</article-id>
<article-id pub-id-type="pmc">4068538</article-id>
<article-id pub-id-type="publisher-id">02695-14</article-id>
<article-id pub-id-type="doi">10.1128/AAC.02695-14</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Mechanisms of Resistance</subject>
</subj-group>
</article-categories>
<title-group><article-title>IncH-Type Plasmid Harboring <italic>bla</italic>
<sub>CTX-M-15</sub>
, <italic>bla</italic>
<sub>DHA-1</sub>
, and <italic>qnrB4</italic>
 Genes Recovered from Animal Isolates</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Schlüter</surname>
<given-names>Andreas</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Nordmann</surname>
<given-names>Patrice</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>b</sup>
</xref>
<xref ref-type="aff" rid="aff3"><sup>c</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Bonnin</surname>
<given-names>Rémy A.</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>b</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Millemann</surname>
<given-names>Yves</given-names>
</name>
<xref ref-type="aff" rid="aff4"><sup>d</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Eikmeyer</surname>
<given-names>Felix G.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wibberg</surname>
<given-names>Daniel</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Pühler</surname>
<given-names>Alfred</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Poirel</surname>
<given-names>Laurent</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>b</sup>
</xref>
<xref ref-type="aff" rid="aff3"><sup>c</sup>
</xref>
</contrib>
<aff id="aff1"><label>a</label>
Center for Biotechnology (CeBiTec), Institute for Genome Research and Systems Biology, Bielefeld University, Bielefeld, Germany</aff>
<aff id="aff2"><label>b</label>
INSERM U914, South-Paris Medical School, Le Kremlin-Bicêtre, France</aff>
<aff id="aff3"><label>c</label>
Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland</aff>
<aff id="aff4"><label>d</label>
Unité Microbiologie Alimentaire, Sécurité et Qualité des Aliments (MASQ), Ecole Nationale Vétérinaire d'Alfort, Université Paris-Est, Maisons-Alfort, France</aff>
</contrib-group>
<author-notes><corresp id="cor1">Address correspondence to Laurent Poirel, <email>laurent.poirel@unifr.ch</email>
.</corresp>
</author-notes>
<pub-date pub-type="ppub"><month>7</month>
<year>2014</year>
</pub-date>
<volume>58</volume>
<issue>7</issue>
<fpage>3768</fpage>
<lpage>3773</lpage>
<history><date date-type="received"><day>3</day>
<month>3</month>
<year>2014</year>
</date>
<date date-type="accepted"><day>13</day>
<month>4</month>
<year>2014</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2014, American Society for Microbiology. All Rights Reserved.</copyright-statement>
<copyright-year>2014</copyright-year>
<copyright-holder>American Society for Microbiology</copyright-holder>
</permissions>
<self-uri xlink:title="pdf" xlink:type="simple" xlink:href="zac00714003768.pdf"></self-uri>
<abstract><p>The whole sequence of plasmid pENVA carrying the extended-spectrum β-lactamase gene <italic>bla</italic>
<sub>CTX-M-15</sub>
 was determined. It was identified from a series of clonally related <named-content content-type="genus-species">Klebsiella pneumoniae</named-content>
 sequence type 274 strains recovered from companion animals. This plasmid was 253,984 bp in size and harbored, in addition to <italic>bla</italic>
<sub>CTX-M-15</sub>
, a large array of genes encoding resistance to many antibiotic molecules, including β-lactams (<italic>bla</italic>
<sub>TEM-1</sub>
, <italic>bla</italic>
<sub>DHA-1</sub>
), aminoglycosides (<italic>aacA2</italic>
, <italic>aadA1</italic>
), tetracycline (<italic>tetA</italic>
), quinolones (<italic>qnrB4</italic>
), trimethoprim (<italic>dfrA15</italic>
), and sulfonamides (two copies of <italic>sul1</italic>
). In addition, genes encoding resistance to mercury, tellurium, nickel, and quaternary compounds were identified. It also carried genes encoding DNA damage protection and mutagenesis repair and a locus for a CRISPR system, which corresponds to an immune system involved in protection against bacteriophages and plasmids. Comparative analysis of the plasmid scaffold showed that it possessed a structure similar to that of only a single plasmid, which was pNDM-MAR encoding the carbapenemase NDM-1 and identified from human <named-content content-type="genus-species">K. pneumoniae</named-content>
 isolates. Both plasmids possessed two replicons, namely, those of IncFIB-like and IncHIB-like plasmids, which were significantly different from those previously characterized. The <italic>bla</italic>
<sub>CTX-M-15</sub>
 gene, together with the other antibiotic resistance genes, was part of a large module likely acquired through a transposition process. We characterized here a new plasmid type carrying the <italic>bla</italic>
<sub>CTX-M-15</sub>
 gene identified in a <named-content content-type="genus-species">K. pneumoniae</named-content>
 isolate of animal origin. The extent to which this plasmid type may spread efficiently and possibly further enhance the dissemination of <italic>bla</italic>
<sub>CTX-M-15</sub>
 among animal and human isolates remains to be determined.</p>
</abstract>
</article-meta>
</front>
</pmc>
</record>

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IncH-Type Plasmid Harboring blaCTX-M-15, blaDHA-1, and qnrB4 Genes Recovered from Animal Isolates

IncH-Type Plasmid Harboring blaCTX-M-15, blaDHA-1, and qnrB4 Genes Recovered from Animal Isolates

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Abstract

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