NickelMaghrebV1, Main, Exploration, bibRecord, 000733

A role for 5,6-epoxyeicosatrienoic acid in calcium entry by de novo conformational coupling in human platelets

Identifieur interne : 000733 ( Main/Exploration ); précédent : 000732; suivant : 000734

A role for 5,6-epoxyeicosatrienoic acid in calcium entry by de novo conformational coupling in human platelets

Auteurs : Nidhal Ben-Amor [Tunisie] ; Pedro C. Redondo [Espagne] ; Aghleb Bartegi [Tunisie] ; José A. Pariente [Espagne] ; Ginés M. Salido [Espagne] ; Juan A. Rosado [Espagne]

Source :

The Journal of Physiology [ 0022-3751 ] ; 2005.

RBID : PMC:1464301

English descriptors

KwdEn :
- 8,11,14-Eicosatrienoic Acid (analogs & derivatives), 8,11,14-Eicosatrienoic Acid (pharmacology), Actins (analysis), Actins (physiology), Antibodies (immunology), Antibodies (pharmacology), Blood Platelets (metabolism), Boron Compounds (pharmacology), Calcium (metabolism), Calcium Channels (physiology), Cell Membrane (chemistry), Cell Membrane (physiology), Cell Membrane Permeability (drug effects), Cytochalasin D (pharmacology), Cytochrome P-450 Enzyme System (physiology), Cytoskeleton (chemistry), Cytoskeleton (drug effects), Cytoskeleton (physiology), Enzyme Activation, Humans, Hydrogen Peroxide (pharmacology), Imidazoles (pharmacology), Inositol 1,4,5-Trisphosphate Receptors, Lanthanum (pharmacology), Manganese (metabolism), Nickel (pharmacology), Protein-Tyrosine Kinases (metabolism), Receptors, Cytoplasmic and Nuclear (physiology), TRPC Cation Channels (immunology), TRPC Cation Channels (physiology), Thapsigargin (pharmacology).
MESH :
- chemical , analogs & derivatives : 8,11,14-Eicosatrienoic Acid.
- chemical , analysis : Actins.
- chemical , immunology : Antibodies, TRPC Cation Channels.
- chemical , metabolism : Calcium, Manganese, Protein-Tyrosine Kinases.
- chemical , pharmacology : 8,11,14-Eicosatrienoic Acid, Antibodies, Boron Compounds, Cytochalasin D, Hydrogen Peroxide, Imidazoles, Lanthanum, Nickel, Thapsigargin.
- chemical , physiology : Actins, Calcium Channels, Cytochrome P-450 Enzyme System, Receptors, Cytoplasmic and Nuclear, TRPC Cation Channels.
- chemistry : Cell Membrane, Cytoskeleton.
- drug effects : Cell Membrane Permeability, Cytoskeleton.
- metabolism : Blood Platelets.
- physiology : Cell Membrane, Cytoskeleton.
- Enzyme Activation, Humans, Inositol 1,4,5-Trisphosphate Receptors.

Abstract

A major pathway for Ca²⁺ entry in non-excitable cells is activated following depletion of intracellular Ca²⁺ stores. A de novo conformational coupling between elements in the plasma membrane (PM) and Ca²⁺ stores has been proposed as the most likely mechanism to activate this capacitative Ca²⁺ entry (CCE) in several cell types, including platelets. Here we report that a cytochrome P450 metabolite, 5,6-EET, might be a component of the de novo conformational coupling in human platelets. In these cells, 5,6-EET induces divalent cation entry without having any detectable effect on Ca²⁺ store depletion. 5,6-EET-induced Ca²⁺ entry was sensitive to the CCE blockers 2-APB, lanthanum, SKF-96365 and nickel and impaired by incubation with anti-hTRPC1 antibody. Ca²⁺ entry stimulated by low concentrations of thapsigargin, which selectively depletes the dense tubular system and induces EET production, was impaired by the cytochrome P450 inhibitor 17-ODYA, which has no effect on CCE mediated by depletion of the acidic stores using 2,5-di-(tert-butyl)-1,4-hydroquinone. We have found that 5,6-EET-induced Ca²⁺ entry requires basal levels of H₂O₂, which might maintain a redox state favourable for this event. Finally, our results indicate that 5,6-EET induces the activation of tyrosine kinase proteins and the reorganization of the actin cytoskeleton, which might provide a support for the transport of portions of the Ca²⁺ store towards the PM to facilitate de novo coupling between IP₃R type II and hTRPC1 detected by coimmunoprecipitation. We propose that the involvement of 5,6-EET in TG-induced coupling between IP₃R type II and hTRPC1 and subsequently CCE is compatible with the de novo conformational coupling in human platelets.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1464301

DOI: 10.1113/jphysiol.2005.100800
PubMed: 16308346
PubMed Central: 1464301

Affiliations:

Espagne, Tunisie

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A role for 5,6-epoxyeicosatrienoic acid in calcium entry by <italic>de novo</italic>
 conformational coupling in human platelets</title>
<author><name sortKey="Ben Amor, Nidhal" sort="Ben Amor, Nidhal" uniqKey="Ben Amor N" first="Nidhal" last="Ben-Amor">Nidhal Ben-Amor</name>
<affiliation wicri:level="1"><nlm:aff id="au1"><institution>Unité de Recherche de Biochimie, Institute Superieur de Biotechnologie</institution>
<addr-line>Monastir, Tunisia</addr-line>
</nlm:aff>
<country xml:lang="fr">Tunisie</country>
<wicri:regionArea>Monastir</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Redondo, Pedro C" sort="Redondo, Pedro C" uniqKey="Redondo P" first="Pedro C" last="Redondo">Pedro C. Redondo</name>
<affiliation wicri:level="1"><nlm:aff id="au2"><institution>Department of Physiology, University of Extremadura</institution>
<addr-line>10071 Cáceres, Spain</addr-line>
</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>10071 Cáceres</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Bartegi, Aghleb" sort="Bartegi, Aghleb" uniqKey="Bartegi A" first="Aghleb" last="Bartegi">Aghleb Bartegi</name>
<affiliation wicri:level="1"><nlm:aff id="au1"><institution>Unité de Recherche de Biochimie, Institute Superieur de Biotechnologie</institution>
<addr-line>Monastir, Tunisia</addr-line>
</nlm:aff>
<country xml:lang="fr">Tunisie</country>
<wicri:regionArea>Monastir</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Pariente, Jose A" sort="Pariente, Jose A" uniqKey="Pariente J" first="José A" last="Pariente">José A. Pariente</name>
<affiliation wicri:level="1"><nlm:aff id="au2"><institution>Department of Physiology, University of Extremadura</institution>
<addr-line>10071 Cáceres, Spain</addr-line>
</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>10071 Cáceres</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Salido, Gines M" sort="Salido, Gines M" uniqKey="Salido G" first="Ginés M" last="Salido">Ginés M. Salido</name>
<affiliation wicri:level="1"><nlm:aff id="au2"><institution>Department of Physiology, University of Extremadura</institution>
<addr-line>10071 Cáceres, Spain</addr-line>
</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>10071 Cáceres</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Rosado, Juan A" sort="Rosado, Juan A" uniqKey="Rosado J" first="Juan A" last="Rosado">Juan A. Rosado</name>
<affiliation wicri:level="1"><nlm:aff id="au2"><institution>Department of Physiology, University of Extremadura</institution>
<addr-line>10071 Cáceres, Spain</addr-line>
</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>10071 Cáceres</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">16308346</idno>
<idno type="pmc">1464301</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1464301</idno>
<idno type="RBID">PMC:1464301</idno>
<idno type="doi">10.1113/jphysiol.2005.100800</idno>
<date when="2005">2005</date>
<idno type="wicri:Area/Pmc/Corpus">000023</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000023</idno>
<idno type="wicri:Area/Pmc/Curation">000023</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000023</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000041</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000041</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="wicri:Area/PubMed/Corpus">000123</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000123</idno>
<idno type="wicri:Area/PubMed/Curation">000123</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000123</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000123</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000123</idno>
<idno type="wicri:Area/Ncbi/Merge">000012</idno>
<idno type="wicri:Area/Ncbi/Curation">000012</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000012</idno>
<idno type="wicri:doubleKey">0022-3751:2005:Ben Amor N:a:role:for</idno>
<idno type="wicri:Area/Main/Merge">000753</idno>
<idno type="wicri:Area/Main/Curation">000733</idno>
<idno type="wicri:Area/Main/Exploration">000733</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A role for 5,6-epoxyeicosatrienoic acid in calcium entry by <italic>de novo</italic>
 conformational coupling in human platelets</title>
<author><name sortKey="Ben Amor, Nidhal" sort="Ben Amor, Nidhal" uniqKey="Ben Amor N" first="Nidhal" last="Ben-Amor">Nidhal Ben-Amor</name>
<affiliation wicri:level="1"><nlm:aff id="au1"><institution>Unité de Recherche de Biochimie, Institute Superieur de Biotechnologie</institution>
<addr-line>Monastir, Tunisia</addr-line>
</nlm:aff>
<country xml:lang="fr">Tunisie</country>
<wicri:regionArea>Monastir</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Redondo, Pedro C" sort="Redondo, Pedro C" uniqKey="Redondo P" first="Pedro C" last="Redondo">Pedro C. Redondo</name>
<affiliation wicri:level="1"><nlm:aff id="au2"><institution>Department of Physiology, University of Extremadura</institution>
<addr-line>10071 Cáceres, Spain</addr-line>
</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>10071 Cáceres</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Bartegi, Aghleb" sort="Bartegi, Aghleb" uniqKey="Bartegi A" first="Aghleb" last="Bartegi">Aghleb Bartegi</name>
<affiliation wicri:level="1"><nlm:aff id="au1"><institution>Unité de Recherche de Biochimie, Institute Superieur de Biotechnologie</institution>
<addr-line>Monastir, Tunisia</addr-line>
</nlm:aff>
<country xml:lang="fr">Tunisie</country>
<wicri:regionArea>Monastir</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Pariente, Jose A" sort="Pariente, Jose A" uniqKey="Pariente J" first="José A" last="Pariente">José A. Pariente</name>
<affiliation wicri:level="1"><nlm:aff id="au2"><institution>Department of Physiology, University of Extremadura</institution>
<addr-line>10071 Cáceres, Spain</addr-line>
</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>10071 Cáceres</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Salido, Gines M" sort="Salido, Gines M" uniqKey="Salido G" first="Ginés M" last="Salido">Ginés M. Salido</name>
<affiliation wicri:level="1"><nlm:aff id="au2"><institution>Department of Physiology, University of Extremadura</institution>
<addr-line>10071 Cáceres, Spain</addr-line>
</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>10071 Cáceres</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Rosado, Juan A" sort="Rosado, Juan A" uniqKey="Rosado J" first="Juan A" last="Rosado">Juan A. Rosado</name>
<affiliation wicri:level="1"><nlm:aff id="au2"><institution>Department of Physiology, University of Extremadura</institution>
<addr-line>10071 Cáceres, Spain</addr-line>
</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>10071 Cáceres</wicri:regionArea>
</affiliation>
</author>
</analytic>
<series><title level="j">The Journal of Physiology</title>
<idno type="ISSN">0022-3751</idno>
<idno type="eISSN">1469-7793</idno>
<imprint><date when="2005">2005</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>8,11,14-Eicosatrienoic Acid (analogs & derivatives)</term>
<term>8,11,14-Eicosatrienoic Acid (pharmacology)</term>
<term>Actins (analysis)</term>
<term>Actins (physiology)</term>
<term>Antibodies (immunology)</term>
<term>Antibodies (pharmacology)</term>
<term>Blood Platelets (metabolism)</term>
<term>Boron Compounds (pharmacology)</term>
<term>Calcium (metabolism)</term>
<term>Calcium Channels (physiology)</term>
<term>Cell Membrane (chemistry)</term>
<term>Cell Membrane (physiology)</term>
<term>Cell Membrane Permeability (drug effects)</term>
<term>Cytochalasin D (pharmacology)</term>
<term>Cytochrome P-450 Enzyme System (physiology)</term>
<term>Cytoskeleton (chemistry)</term>
<term>Cytoskeleton (drug effects)</term>
<term>Cytoskeleton (physiology)</term>
<term>Enzyme Activation</term>
<term>Humans</term>
<term>Hydrogen Peroxide (pharmacology)</term>
<term>Imidazoles (pharmacology)</term>
<term>Inositol 1,4,5-Trisphosphate Receptors</term>
<term>Lanthanum (pharmacology)</term>
<term>Manganese (metabolism)</term>
<term>Nickel (pharmacology)</term>
<term>Protein-Tyrosine Kinases (metabolism)</term>
<term>Receptors, Cytoplasmic and Nuclear (physiology)</term>
<term>TRPC Cation Channels (immunology)</term>
<term>TRPC Cation Channels (physiology)</term>
<term>Thapsigargin (pharmacology)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>8,11,14-Eicosatrienoic Acid</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Actins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies</term>
<term>TRPC Cation Channels</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Calcium</term>
<term>Manganese</term>
<term>Protein-Tyrosine Kinases</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>8,11,14-Eicosatrienoic Acid</term>
<term>Antibodies</term>
<term>Boron Compounds</term>
<term>Cytochalasin D</term>
<term>Hydrogen Peroxide</term>
<term>Imidazoles</term>
<term>Lanthanum</term>
<term>Nickel</term>
<term>Thapsigargin</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Actins</term>
<term>Calcium Channels</term>
<term>Cytochrome P-450 Enzyme System</term>
<term>Receptors, Cytoplasmic and Nuclear</term>
<term>TRPC Cation Channels</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Cell Membrane</term>
<term>Cytoskeleton</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Membrane Permeability</term>
<term>Cytoskeleton</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Blood Platelets</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Cell Membrane</term>
<term>Cytoskeleton</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Enzyme Activation</term>
<term>Humans</term>
<term>Inositol 1,4,5-Trisphosphate Receptors</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>A major pathway for Ca<sup>2+</sup>
 entry in non-excitable cells is activated following depletion of intracellular Ca<sup>2+</sup>
 stores. A <italic>de novo</italic>
 conformational coupling between elements in the plasma membrane (PM) and Ca<sup>2+</sup>
 stores has been proposed as the most likely mechanism to activate this capacitative Ca<sup>2+</sup>
 entry (CCE) in several cell types, including platelets. Here we report that a cytochrome P450 metabolite, 5,6-EET, might be a component of the <italic>de novo</italic>
 conformational coupling in human platelets. In these cells, 5,6-EET induces divalent cation entry without having any detectable effect on Ca<sup>2+</sup>
 store depletion. 5,6-EET-induced Ca<sup>2+</sup>
 entry was sensitive to the CCE blockers 2-APB, lanthanum, SKF-96365 and nickel and impaired by incubation with anti-hTRPC1 antibody. Ca<sup>2+</sup>
 entry stimulated by low concentrations of thapsigargin, which selectively depletes the dense tubular system and induces EET production, was impaired by the cytochrome P450 inhibitor 17-ODYA, which has no effect on CCE mediated by depletion of the acidic stores using 2,5-di-(tert-butyl)-1,4-hydroquinone. We have found that 5,6-EET-induced Ca<sup>2+</sup>
 entry requires basal levels of H<sub>2</sub>
O<sub>2</sub>
, which might maintain a redox state favourable for this event. Finally, our results indicate that 5,6-EET induces the activation of tyrosine kinase proteins and the reorganization of the actin cytoskeleton, which might provide a support for the transport of portions of the Ca<sup>2+</sup>
 store towards the PM to facilitate <italic>de novo</italic>
 coupling between IP<sub>3</sub>
R type II and hTRPC1 detected by coimmunoprecipitation. We propose that the involvement of 5,6-EET in TG-induced coupling between IP<sub>3</sub>
R type II and hTRPC1 and subsequently CCE is compatible with the <italic>de novo</italic>
 conformational coupling in human platelets.</p>
</div>
</front>
</TEI>
<affiliations><list><country><li>Espagne</li>
<li>Tunisie</li>
</country>
</list>
<tree><country name="Tunisie"><noRegion><name sortKey="Ben Amor, Nidhal" sort="Ben Amor, Nidhal" uniqKey="Ben Amor N" first="Nidhal" last="Ben-Amor">Nidhal Ben-Amor</name>
</noRegion>
<name sortKey="Bartegi, Aghleb" sort="Bartegi, Aghleb" uniqKey="Bartegi A" first="Aghleb" last="Bartegi">Aghleb Bartegi</name>
</country>
<country name="Espagne"><noRegion><name sortKey="Redondo, Pedro C" sort="Redondo, Pedro C" uniqKey="Redondo P" first="Pedro C" last="Redondo">Pedro C. Redondo</name>
</noRegion>
<name sortKey="Pariente, Jose A" sort="Pariente, Jose A" uniqKey="Pariente J" first="José A" last="Pariente">José A. Pariente</name>
<name sortKey="Rosado, Juan A" sort="Rosado, Juan A" uniqKey="Rosado J" first="Juan A" last="Rosado">Juan A. Rosado</name>
<name sortKey="Salido, Gines M" sort="Salido, Gines M" uniqKey="Salido G" first="Ginés M" last="Salido">Ginés M. Salido</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Terre/explor/NickelMaghrebV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000733 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000733 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Terre
   |area=    NickelMaghrebV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     PMC:1464301
   |texte=   A role for 5,6-epoxyeicosatrienoic acid in calcium entry by de novo conformational coupling in human platelets
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:16308346" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a NickelMaghrebV1

This area was generated with Dilib version V0.6.27.
Data generation: Fri Mar 24 23:14:20 2017. Site generation: Tue Mar 5 17:03:47 2024

Serveur d'exploration sur le nickel au Maghreb

A role for 5,6-epoxyeicosatrienoic acid in calcium entry by de novo conformational coupling in human platelets

A role for 5,6-epoxyeicosatrienoic acid in calcium entry by de novo conformational coupling in human platelets

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration sur le nickel au Maghreb
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.