Hemoglobin linked to polyanionic polymers as potential red blood cell substitutes.
Identifieur interne : 000261 ( PubMed/Corpus ); précédent : 000260; suivant : 000262Hemoglobin linked to polyanionic polymers as potential red blood cell substitutes.
Auteurs : E. Dellacherie ; M. Grandgeorge ; F. Prouchayret ; G. FasanSource :
- Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology [ 1055-7172 ] ; 1992.
English descriptors
- KwdEn :
- MESH :
- chemical , chemical synthesis : Blood Substitutes, Hemoglobins.
- chemical , chemistry : Blood Substitutes, Hemoglobins.
- chemical : Dextrans, Oxygen, Polyethylene Glycols.
- Drug Stability, Humans, Molecular Weight.
Abstract
A variety of chemical modifications of hemoglobin (Hb) have been proposed in order to transform it into cell-free oxygen carriers. These modifications are intended to increase the plasmatic half-life of the protein and to lower its affinity for oxygen. We have designed and prepared derivatives of dextran and polyoxyethylene functionalized so that, after their covalent fixation onto Hb, they can act as permanent effectors and thus lower the oxygen affinity of the protein while decreasing its renal excretion. The main characteristic of these functionalized polymers is that they possess a relatively high density of anionic groups. Benzene hexacarboxylate (BHC) and tetracarboxylate (BTC) linked to water-soluble polymers such as polyoxyethylene or dextran, yielded polymeric derivatives which, even after reaction with oxyHb, gave rise to conjugates with a lower oxygen affinity than the native protein. We showed on a conjugate obtained by the fixation of a BHC-monosubstituted polyoxyethylene onto oxyHb, that the low oxygen affinity was due to the preferential binding of the polymer-linked BHC to the beta-terminal valine residues. In the reaction of dextran-linked benzene tetracarboxylate (dex-BTC) with oxyHb, a lot of parameters had to be optimized in order to obtain conjugates well fitted to the purpose of blood substitute. Thus the polymer content in BTC and the amounts of reagents used for the reaction determined the oxygen-binding properties, the molecular weights and the biochemical characteristics of the conjugates, as well as the viscosity and oncotic pressure of the solutions. This optimization resulted in products which are now studied in-vivo.
PubMed: 1382640
Links to Exploration step
pubmed:1382640Le document en format XML
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Fasan</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1992">1992</date><idno type="RBID">pubmed:1382640</idno><idno type="pmid">1382640</idno><idno type="wicri:Area/PubMed/Corpus">000261</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000261</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Hemoglobin linked to polyanionic polymers as potential red blood cell substitutes.</title><author><name sortKey="Dellacherie, E" sort="Dellacherie, E" uniqKey="Dellacherie E" first="E" last="Dellacherie">E. Dellacherie</name><affiliation><nlm:affiliation>Laboratoire de Chimie-Physique Macromoléculaire, URA CNRS 494, ENSIC, Nancy, France.</nlm:affiliation></affiliation></author><author><name sortKey="Grandgeorge, M" sort="Grandgeorge, M" uniqKey="Grandgeorge M" first="M" last="Grandgeorge">M. Grandgeorge</name></author><author><name sortKey="Prouchayret, F" sort="Prouchayret, F" uniqKey="Prouchayret F" first="F" last="Prouchayret">F. Prouchayret</name></author><author><name sortKey="Fasan, G" sort="Fasan, G" uniqKey="Fasan G" first="G" last="Fasan">G. Fasan</name></author></analytic><series><title level="j">Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology</title><idno type="ISSN">1055-7172</idno><imprint><date when="1992" type="published">1992</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Blood Substitutes (chemical synthesis)</term><term>Blood Substitutes (chemistry)</term><term>Dextrans</term><term>Drug Stability</term><term>Hemoglobins (chemical synthesis)</term><term>Hemoglobins (chemistry)</term><term>Humans</term><term>Molecular Weight</term><term>Oxygen</term><term>Polyethylene Glycols</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemical synthesis" xml:lang="en"><term>Blood Substitutes</term><term>Hemoglobins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Blood Substitutes</term><term>Hemoglobins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Dextrans</term><term>Oxygen</term><term>Polyethylene Glycols</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Drug Stability</term><term>Humans</term><term>Molecular Weight</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A variety of chemical modifications of hemoglobin (Hb) have been proposed in order to transform it into cell-free oxygen carriers. These modifications are intended to increase the plasmatic half-life of the protein and to lower its affinity for oxygen. We have designed and prepared derivatives of dextran and polyoxyethylene functionalized so that, after their covalent fixation onto Hb, they can act as permanent effectors and thus lower the oxygen affinity of the protein while decreasing its renal excretion. The main characteristic of these functionalized polymers is that they possess a relatively high density of anionic groups. Benzene hexacarboxylate (BHC) and tetracarboxylate (BTC) linked to water-soluble polymers such as polyoxyethylene or dextran, yielded polymeric derivatives which, even after reaction with oxyHb, gave rise to conjugates with a lower oxygen affinity than the native protein. We showed on a conjugate obtained by the fixation of a BHC-monosubstituted polyoxyethylene onto oxyHb, that the low oxygen affinity was due to the preferential binding of the polymer-linked BHC to the beta-terminal valine residues. In the reaction of dextran-linked benzene tetracarboxylate (dex-BTC) with oxyHb, a lot of parameters had to be optimized in order to obtain conjugates well fitted to the purpose of blood substitute. Thus the polymer content in BTC and the amounts of reagents used for the reaction determined the oxygen-binding properties, the molecular weights and the biochemical characteristics of the conjugates, as well as the viscosity and oncotic pressure of the solutions. This optimization resulted in products which are now studied in-vivo.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">1382640</PMID><DateCreated><Year>1992</Year><Month>11</Month><Day>18</Day></DateCreated><DateCompleted><Year>1992</Year><Month>11</Month><Day>18</Day></DateCompleted><DateRevised><Year>2015</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1055-7172</ISSN><JournalIssue CitedMedium="Print"><Volume>20</Volume><Issue>2-4</Issue><PubDate><Year>1992</Year></PubDate></JournalIssue><Title>Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology</Title><ISOAbbreviation>Biomater Artif Cells Immobilization Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Hemoglobin linked to polyanionic polymers as potential red blood cell substitutes.</ArticleTitle><Pagination><MedlinePgn>309-17</MedlinePgn></Pagination><Abstract><AbstractText>A variety of chemical modifications of hemoglobin (Hb) have been proposed in order to transform it into cell-free oxygen carriers. These modifications are intended to increase the plasmatic half-life of the protein and to lower its affinity for oxygen. We have designed and prepared derivatives of dextran and polyoxyethylene functionalized so that, after their covalent fixation onto Hb, they can act as permanent effectors and thus lower the oxygen affinity of the protein while decreasing its renal excretion. The main characteristic of these functionalized polymers is that they possess a relatively high density of anionic groups. Benzene hexacarboxylate (BHC) and tetracarboxylate (BTC) linked to water-soluble polymers such as polyoxyethylene or dextran, yielded polymeric derivatives which, even after reaction with oxyHb, gave rise to conjugates with a lower oxygen affinity than the native protein. We showed on a conjugate obtained by the fixation of a BHC-monosubstituted polyoxyethylene onto oxyHb, that the low oxygen affinity was due to the preferential binding of the polymer-linked BHC to the beta-terminal valine residues. In the reaction of dextran-linked benzene tetracarboxylate (dex-BTC) with oxyHb, a lot of parameters had to be optimized in order to obtain conjugates well fitted to the purpose of blood substitute. Thus the polymer content in BTC and the amounts of reagents used for the reaction determined the oxygen-binding properties, the molecular weights and the biochemical characteristics of the conjugates, as well as the viscosity and oncotic pressure of the solutions. This optimization resulted in products which are now studied in-vivo.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Dellacherie</LastName><ForeName>E</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Laboratoire de Chimie-Physique Macromoléculaire, URA CNRS 494, ENSIC, Nancy, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Grandgeorge</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Prouchayret</LastName><ForeName>F</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Fasan</LastName><ForeName>G</ForeName><Initials>G</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>UNITED STATES</Country><MedlineTA>Biomater Artif Cells Immobilization Biotechnol</MedlineTA><NlmUniqueID>9111988</NlmUniqueID><ISSNLinking>1055-7172</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001802">Blood Substitutes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006454">Hemoglobins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C037158">polyhemoglobin</NameOfSubstance></Chemical><Chemical><RegistryNumber>30IQX730WE</RegistryNumber><NameOfSubstance UI="D011092">Polyethylene Glycols</NameOfSubstance></Chemical><Chemical><RegistryNumber>K3R6ZDH4DU</RegistryNumber><NameOfSubstance UI="D003911">Dextrans</NameOfSubstance></Chemical><Chemical><RegistryNumber>S88TT14065</RegistryNumber><NameOfSubstance UI="D010100">Oxygen</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N" UI="D001802">Blood Substitutes</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000138">chemical synthesis</QualifierName><QualifierName MajorTopicYN="N" UI="Q000737">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D003911">Dextrans</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D004355">Drug Stability</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D006454">Hemoglobins</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000138">chemical synthesis</QualifierName><QualifierName MajorTopicYN="N" UI="Q000737">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D006801">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D008970">Molecular Weight</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D010100">Oxygen</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D011092">Polyethylene Glycols</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1992</Year><Month>1</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1992</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1992</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">1382640</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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