Genotypic and Chemotypic Diversity of Neotyphodium Endophytes in Tall Fescue from Greece
Identifieur interne : 000121 ( Pmc/Curation ); précédent : 000120; suivant : 000122Genotypic and Chemotypic Diversity of Neotyphodium Endophytes in Tall Fescue from Greece
Auteurs : Johanna E. Takach ; Shipra Mittal ; Ginger A. Swoboda ; Sherrita K. Bright ; Michael A. Trammell ; Andrew A. Hopkins ; Carolyn A. YoungSource :
- Applied and Environmental Microbiology [ 0099-2240 ] ; 2012.
Abstract
Epichloid endophytes provide protection from a variety of biotic and abiotic stresses for cool-season grasses, including tall fescue. A collection of 85 tall fescue lines from 15 locations in Greece, including both Continental and Mediterranean germplasm, was screened for the presence of native endophytes. A total of 37 endophyte-infected lines from 10 locations were identified, and the endophytes were classified into five distinct groups (G1 to G5) based on physical characteristics such as colony morphology, growth rate, and conidial morphology. These classifications were supported by phylogenetic analyses of housekeeping genes
Url:
DOI: 10.1128/AEM.01084-12
PubMed: 22660705
PubMed Central: 3406137
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Genotypic and Chemotypic Diversity of <named-content content-type="genus-species">Neotyphodium</named-content>
Endophytes in Tall Fescue from Greece</title>
<author><name sortKey="Takach, Johanna E" sort="Takach, Johanna E" uniqKey="Takach J" first="Johanna E." last="Takach">Johanna E. Takach</name>
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<author><name sortKey="Mittal, Shipra" sort="Mittal, Shipra" uniqKey="Mittal S" first="Shipra" last="Mittal">Shipra Mittal</name>
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<author><name sortKey="Swoboda, Ginger A" sort="Swoboda, Ginger A" uniqKey="Swoboda G" first="Ginger A." last="Swoboda">Ginger A. Swoboda</name>
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<author><name sortKey="Bright, Sherrita K" sort="Bright, Sherrita K" uniqKey="Bright S" first="Sherrita K." last="Bright">Sherrita K. Bright</name>
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<author><name sortKey="Trammell, Michael A" sort="Trammell, Michael A" uniqKey="Trammell M" first="Michael A." last="Trammell">Michael A. Trammell</name>
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<author><name sortKey="Hopkins, Andrew A" sort="Hopkins, Andrew A" uniqKey="Hopkins A" first="Andrew A." last="Hopkins">Andrew A. Hopkins</name>
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<author><name sortKey="Young, Carolyn A" sort="Young, Carolyn A" uniqKey="Young C" first="Carolyn A." last="Young">Carolyn A. Young</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Genotypic and Chemotypic Diversity of <named-content content-type="genus-species">Neotyphodium</named-content>
Endophytes in Tall Fescue from Greece</title>
<author><name sortKey="Takach, Johanna E" sort="Takach, Johanna E" uniqKey="Takach J" first="Johanna E." last="Takach">Johanna E. Takach</name>
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<author><name sortKey="Swoboda, Ginger A" sort="Swoboda, Ginger A" uniqKey="Swoboda G" first="Ginger A." last="Swoboda">Ginger A. Swoboda</name>
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<author><name sortKey="Bright, Sherrita K" sort="Bright, Sherrita K" uniqKey="Bright S" first="Sherrita K." last="Bright">Sherrita K. Bright</name>
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<author><name sortKey="Trammell, Michael A" sort="Trammell, Michael A" uniqKey="Trammell M" first="Michael A." last="Trammell">Michael A. Trammell</name>
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<author><name sortKey="Hopkins, Andrew A" sort="Hopkins, Andrew A" uniqKey="Hopkins A" first="Andrew A." last="Hopkins">Andrew A. Hopkins</name>
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<series><title level="j">Applied and Environmental Microbiology</title>
<idno type="ISSN">0099-2240</idno>
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<imprint><date when="2012">2012</date>
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<front><div type="abstract" xml:lang="en"><p>Epichloid endophytes provide protection from a variety of biotic and abiotic stresses for cool-season grasses, including tall fescue. A collection of 85 tall fescue lines from 15 locations in Greece, including both Continental and Mediterranean germplasm, was screened for the presence of native endophytes. A total of 37 endophyte-infected lines from 10 locations were identified, and the endophytes were classified into five distinct groups (G1 to G5) based on physical characteristics such as colony morphology, growth rate, and conidial morphology. These classifications were supported by phylogenetic analyses of housekeeping genes <italic>tefA</italic>
and <italic>tubB</italic>
, and the endophytes were further categorized as <named-content content-type="genus-species">Neotyphodium coenophialum</named-content>
isolates (G1, G4, and G5) or <named-content content-type="genus-species">Neotyphodium</named-content>
sp. FaTG-2 (<named-content content-type="genus-species">Festuca arundinacea</named-content>
taxonomic group 2 isolates (G2 and G3). Analyses of the tall fescue <italic>matK</italic>
chloroplast genes indicated a population-wide, host-specific association between <named-content content-type="genus-species">N. coenophialum</named-content>
and Continental tall fescue and between FaTG-2 and Mediterranean tall fescue that was also reflected by differences in colonization of host tillers by the native endophytes. Genotypic analyses of alkaloid gene loci combined with chemotypic (chemical phenotype) profiles provided insight into the genetic basis of chemotype diversity. Variation in alkaloid gene content, specifically the presence and absence of genes, and copy number of gene clusters explained the alkaloid diversity observed in the endophyte-infected tall fescue, with one exception. The results from this study provide insight into endophyte germplasm diversity present in living tall fescue populations.</p>
</div>
</front>
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<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Appl Environ Microbiol</journal-id>
<journal-id journal-id-type="iso-abbrev">Appl. Environ. Microbiol</journal-id>
<journal-id journal-id-type="hwp">aem</journal-id>
<journal-id journal-id-type="pmc">aem</journal-id>
<journal-id journal-id-type="publisher-id">AEM</journal-id>
<journal-title-group><journal-title>Applied and Environmental Microbiology</journal-title>
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<issn pub-type="ppub">0099-2240</issn>
<issn pub-type="epub">1098-5336</issn>
<publisher><publisher-name>American Society for Microbiology</publisher-name>
<publisher-loc>1752 N St., N.W., Washington, DC</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">22660705</article-id>
<article-id pub-id-type="pmc">3406137</article-id>
<article-id pub-id-type="publisher-id">01084-12</article-id>
<article-id pub-id-type="doi">10.1128/AEM.01084-12</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Genetics and Molecular Biology</subject>
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</article-categories>
<title-group><article-title>Genotypic and Chemotypic Diversity of <named-content content-type="genus-species">Neotyphodium</named-content>
Endophytes in Tall Fescue from Greece</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Takach</surname>
<given-names>Johanna E.</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Mittal</surname>
<given-names>Shipra</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Swoboda</surname>
<given-names>Ginger A.</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Bright</surname>
<given-names>Sherrita K.</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Trammell</surname>
<given-names>Michael A.</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Hopkins</surname>
<given-names>Andrew A.</given-names>
</name>
<xref ref-type="author-notes" rid="FN1">*</xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Young</surname>
<given-names>Carolyn A.</given-names>
</name>
</contrib>
<aff>Forage Improvement Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma, USA</aff>
</contrib-group>
<author-notes><corresp>Address correspondence to Carolyn A. Young, <email>cayoung@noble.org</email>
.</corresp>
<fn id="FN1" fn-type="present-address"><label>*</label>
<p>Present address: Andrew A. Hopkins, Dow AgroSciences, Inc., York, Nebraska, USA.</p>
</fn>
</author-notes>
<pub-date pub-type="ppub"><month>8</month>
<year>2012</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>15</day>
<month>8</month>
<year>2012</year>
</pub-date>
<pmc-comment> PMC Release delay is 0 months and 0 days and was based on the
. </pmc-comment>
<volume>78</volume>
<issue>16</issue>
<fpage>5501</fpage>
<lpage>5510</lpage>
<history><date date-type="received"><day>2</day>
<month>4</month>
<year>2012</year>
</date>
<date date-type="accepted"><day>13</day>
<month>5</month>
<year>2012</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2012, American Society for Microbiology. All Rights Reserved.</copyright-statement>
<copyright-year>2012</copyright-year>
<copyright-holder>American Society for Microbiology</copyright-holder>
<license license-type="open-access"><license-p>The authors have paid a fee to allow immediate free access to this article.</license-p>
</license>
</permissions>
<self-uri xlink:title="pdf" xlink:type="simple" xlink:href="zam01612005501.pdf"></self-uri>
<abstract><p>Epichloid endophytes provide protection from a variety of biotic and abiotic stresses for cool-season grasses, including tall fescue. A collection of 85 tall fescue lines from 15 locations in Greece, including both Continental and Mediterranean germplasm, was screened for the presence of native endophytes. A total of 37 endophyte-infected lines from 10 locations were identified, and the endophytes were classified into five distinct groups (G1 to G5) based on physical characteristics such as colony morphology, growth rate, and conidial morphology. These classifications were supported by phylogenetic analyses of housekeeping genes <italic>tefA</italic>
and <italic>tubB</italic>
, and the endophytes were further categorized as <named-content content-type="genus-species">Neotyphodium coenophialum</named-content>
isolates (G1, G4, and G5) or <named-content content-type="genus-species">Neotyphodium</named-content>
sp. FaTG-2 (<named-content content-type="genus-species">Festuca arundinacea</named-content>
taxonomic group 2 isolates (G2 and G3). Analyses of the tall fescue <italic>matK</italic>
chloroplast genes indicated a population-wide, host-specific association between <named-content content-type="genus-species">N. coenophialum</named-content>
and Continental tall fescue and between FaTG-2 and Mediterranean tall fescue that was also reflected by differences in colonization of host tillers by the native endophytes. Genotypic analyses of alkaloid gene loci combined with chemotypic (chemical phenotype) profiles provided insight into the genetic basis of chemotype diversity. Variation in alkaloid gene content, specifically the presence and absence of genes, and copy number of gene clusters explained the alkaloid diversity observed in the endophyte-infected tall fescue, with one exception. The results from this study provide insight into endophyte germplasm diversity present in living tall fescue populations.</p>
</abstract>
</article-meta>
</front>
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