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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Involvement of <italic>Neisseria meningitidis</italic> Lipoprotein GNA2091 in the Assembly of a Subset of Outer Membrane Proteins</title><author><name sortKey="Bos, Martine P" sort="Bos, Martine P" uniqKey="Bos M" first="Martine P." last="Bos">Martine P. Bos</name><affiliation><nlm:aff id="aff1">From the Department of Molecular Microbiology, Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Grijpstra, Jan" sort="Grijpstra, Jan" uniqKey="Grijpstra J" first="Jan" last="Grijpstra">Jan Grijpstra</name><affiliation><nlm:aff id="aff1">From the Department of Molecular Microbiology, Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Tommassen Van Boxtel, Ria" sort="Tommassen Van Boxtel, Ria" uniqKey="Tommassen Van Boxtel R" first="Ria" last="Tommassen-Van Boxtel">Ria Tommassen-Van Boxtel</name><affiliation><nlm:aff id="aff1">From the Department of Molecular Microbiology, Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Tommassen, Jan" sort="Tommassen, Jan" uniqKey="Tommassen J" first="Jan" last="Tommassen">Jan Tommassen</name><affiliation><nlm:aff id="aff1">From the Department of Molecular Microbiology, Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24755216</idno><idno type="pmc">4140915</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4140915</idno><idno type="RBID">PMC:4140915</idno><idno type="doi">10.1074/jbc.M113.539510</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000131</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000131</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Involvement of <italic>Neisseria meningitidis</italic> Lipoprotein GNA2091 in the Assembly of a Subset of Outer Membrane Proteins</title><author><name sortKey="Bos, Martine P" sort="Bos, Martine P" uniqKey="Bos M" first="Martine P." last="Bos">Martine P. Bos</name><affiliation><nlm:aff id="aff1">From the Department of Molecular Microbiology, Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Grijpstra, Jan" sort="Grijpstra, Jan" uniqKey="Grijpstra J" first="Jan" last="Grijpstra">Jan Grijpstra</name><affiliation><nlm:aff id="aff1">From the Department of Molecular Microbiology, Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Tommassen Van Boxtel, Ria" sort="Tommassen Van Boxtel, Ria" uniqKey="Tommassen Van Boxtel R" first="Ria" last="Tommassen-Van Boxtel">Ria Tommassen-Van Boxtel</name><affiliation><nlm:aff id="aff1">From the Department of Molecular Microbiology, Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Tommassen, Jan" sort="Tommassen, Jan" uniqKey="Tommassen J" first="Jan" last="Tommassen">Jan Tommassen</name><affiliation><nlm:aff id="aff1">From the Department of Molecular Microbiology, Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands</nlm:aff></affiliation></author></analytic><series><title level="j">The Journal of Biological Chemistry</title><idno type="ISSN">0021-9258</idno><idno type="eISSN">1083-351X</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><bold>Background:</bold> Outer membrane protein assembly is an incompletely understood process in Gram-negative bacteria.</p><p><bold>Results:</bold> A <italic>Neisseria meningitidis</italic> mutant lacking the lipoprotein GNA2091 is affected in growth and accumulates unassembled outer membrane proteins.</p><p><bold>Conclusion:</bold> We identified a novel component involved in outer membrane biogenesis.</p><p><bold>Significance:</bold> Our findings contribute to the understanding of a fundamental process occurring in Gram-negative bacteria.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Biol Chem</journal-id><journal-id journal-id-type="iso-abbrev">J. Biol. Chem</journal-id><journal-id journal-id-type="hwp">jbc</journal-id><journal-id journal-id-type="pmc">jbc</journal-id><journal-id journal-id-type="publisher-id">JBC</journal-id><journal-title-group><journal-title>The Journal of Biological Chemistry</journal-title></journal-title-group><issn pub-type="ppub">0021-9258</issn><issn pub-type="epub">1083-351X</issn><publisher><publisher-name>American Society for Biochemistry and Molecular Biology</publisher-name><publisher-loc>9650 Rockville Pike, Bethesda, MD 20814, U.S.A.</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">24755216</article-id><article-id pub-id-type="pmc">4140915</article-id><article-id pub-id-type="publisher-id">M113.539510</article-id><article-id pub-id-type="doi">10.1074/jbc.M113.539510</article-id><article-categories><subj-group subj-group-type="heading"><subject>Microbiology</subject></subj-group></article-categories><title-group><article-title>Involvement of <italic>Neisseria meningitidis</italic> Lipoprotein GNA2091 in the Assembly of a Subset of Outer Membrane Proteins</article-title><alt-title alt-title-type="short">Role of GNA2091 in OMP Assembly</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Bos</surname><given-names>Martine P.</given-names></name><xref ref-type="aff" rid="aff1"></xref><xref ref-type="corresp" rid="cor1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Grijpstra</surname><given-names>Jan</given-names></name><xref ref-type="aff" rid="aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Tommassen-van Boxtel</surname><given-names>Ria</given-names></name><xref ref-type="aff" rid="aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Tommassen</surname><given-names>Jan</given-names></name><xref ref-type="aff" rid="aff1"></xref></contrib><aff id="aff1">From the Department of Molecular Microbiology, Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands</aff></contrib-group><author-notes><corresp id="cor1"><label>1</label> To whom correspondence should be addressed: <addr-line>Dept. of Molecular Microbiology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.</addr-line> Tel.: <phone>31-30-2532999</phone>; Fax: <fax>31-30-2532837</fax>; E-mail: <email>m.bos@uu.nl</email>.</corresp></author-notes><pub-date pub-type="ppub"><day>30</day><month>5</month><year>2014</year></pub-date><pub-date pub-type="epub"><day>22</day><month>4</month><year>2014</year></pub-date><volume>289</volume><issue>22</issue><fpage>15602</fpage><lpage>15610</lpage><history><date date-type="received"><day>30</day><month>11</month><year>2013</year></date><date date-type="rev-recd"><day>18</day><month>4</month><year>2014</year></date></history><permissions><copyright-statement>© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.</copyright-statement><copyright-year>2014</copyright-year></permissions><self-uri xlink:title="pdf" xlink:type="simple" xlink:href="zbc02214015602.pdf"></self-uri><abstract abstract-type="teaser"><p><bold>Background:</bold> Outer membrane protein assembly is an incompletely understood process in Gram-negative bacteria.</p><p><bold>Results:</bold> A <italic>Neisseria meningitidis</italic> mutant lacking the lipoprotein GNA2091 is affected in growth and accumulates unassembled outer membrane proteins.</p><p><bold>Conclusion:</bold> We identified a novel component involved in outer membrane biogenesis.</p><p><bold>Significance:</bold> Our findings contribute to the understanding of a fundamental process occurring in Gram-negative bacteria.</p></abstract><abstract><p>GNA2091 of <italic>Neisseria meningitidis</italic> is a lipoprotein of unknown function that is included in the novel 4CMenB vaccine. Here, we investigated the biological function and the subcellular localization of the protein. We demonstrate that GNA2091 functions in the assembly of outer membrane proteins (OMPs) because its absence resulted in the accumulation of misassembled OMPs. Cell fractionation and protease accessibility experiments showed that the protein is localized at the periplasmic side of the outer membrane. Pulldown experiments revealed that it is not stably associated with the β-barrel assembly machinery, the previously identified complex for OMP assembly. Thus, GNA2091 constitutes a novel outer membrane-based lipoprotein required for OMP assembly. Furthermore, its location at the inner side of the outer membrane indicates that protective immunity elicited by this antigen cannot be due to bactericidal or opsonic activity of antibodies.</p></abstract><kwd-group><kwd>Bacteria</kwd><kwd>Membrane Biogenesis</kwd><kwd>Membrane Protein</kwd><kwd>Microbiology</kwd><kwd>Vaccine</kwd><kwd>BAM Complex</kwd><kwd>Neisseria meningitidis</kwd><kwd>YraP</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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