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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Derivation of 3D coordinate templates for searching structural databases: application to Ser-His-Asp catalytic triads in the serine proteinases and lipases.</title><author><name sortKey="Wallace, A C" sort="Wallace, A C" uniqKey="Wallace A" first="A. C." last="Wallace">A. C. Wallace</name></author><author><name sortKey="Laskowski, R A" sort="Laskowski, R A" uniqKey="Laskowski R" first="R. A." last="Laskowski">R. A. Laskowski</name></author><author><name sortKey="Thornton, J M" sort="Thornton, J M" uniqKey="Thornton J" first="J. M." last="Thornton">J. M. Thornton</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">8762132</idno><idno type="pmc">2143436</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2143436</idno><idno type="RBID">PMC:2143436</idno><date when="1996">1996</date><idno type="wicri:Area/Pmc/Corpus">000095</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000095</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Derivation of 3D coordinate templates for searching structural databases: application to Ser-His-Asp catalytic triads in the serine proteinases and lipases.</title><author><name sortKey="Wallace, A C" sort="Wallace, A C" uniqKey="Wallace A" first="A. C." last="Wallace">A. C. Wallace</name></author><author><name sortKey="Laskowski, R A" sort="Laskowski, R A" uniqKey="Laskowski R" first="R. A." last="Laskowski">R. A. Laskowski</name></author><author><name sortKey="Thornton, J M" sort="Thornton, J M" uniqKey="Thornton J" first="J. M." last="Thornton">J. M. Thornton</name></author></analytic><series><title level="j">Protein Science : A Publication of the Protein Society</title><idno type="ISSN">0961-8368</idno><idno type="eISSN">1469-896X</idno><imprint><date when="1996">1996</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>It is well established that sequence templates (e.g., PROSITE) and databases are powerful tools for identifying biological function and tertiary structure for an unknown protein sequence. Here we describe a method for automatically deriving 3D templates from the protein structures deposited in the Brookhaven Protein Data Bank. As an example, we describe a template derived for the Ser-His-Asp catalytic triad found in the serine proteases and triacylglycerol lipases. We find that the resultant template provides a highly selective tool for automatically differentiating between catalytic and noncatalytic Ser-His-Asp associations. When applied to nonproteolytic proteins, the template picks out two "non-esterase" catalytic triads that may be of biological relevance. This suggests that the development of databases of 3D templates, such as those that currently exist for protein sequence templates, will help identify the functions of new protein structures as they are determined and pinpoint their functionally important regions.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Protein Sci</journal-id><journal-id journal-id-type="pmc">prosci</journal-id><journal-title>Protein Science : A Publication of the Protein Society</journal-title><issn pub-type="ppub">0961-8368</issn><issn pub-type="epub">1469-896X</issn><publisher><publisher-name>Cold Spring Harbor Laboratory Press</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">8762132</article-id><article-id pub-id-type="pmc">2143436</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Derivation of 3D coordinate templates for searching structural databases: application to Ser-His-Asp catalytic triads in the serine proteinases and lipases.</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Wallace</surname><given-names>A. C.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Laskowski</surname><given-names>R. A.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Thornton</surname><given-names>J. M.</given-names></name></contrib></contrib-group><aff>Department of Biochemistry and Molecular Biology, University College, London, England.</aff><pub-date pub-type="ppub"><month>6</month><year>1996</year></pub-date><volume>5</volume><issue>6</issue><fpage>1001</fpage><lpage>1013</lpage><abstract><p>It is well established that sequence templates (e.g., PROSITE) and databases are powerful tools for identifying biological function and tertiary structure for an unknown protein sequence. Here we describe a method for automatically deriving 3D templates from the protein structures deposited in the Brookhaven Protein Data Bank. As an example, we describe a template derived for the Ser-His-Asp catalytic triad found in the serine proteases and triacylglycerol lipases. We find that the resultant template provides a highly selective tool for automatically differentiating between catalytic and noncatalytic Ser-His-Asp associations. When applied to nonproteolytic proteins, the template picks out two "non-esterase" catalytic triads that may be of biological relevance. This suggests that the development of databases of 3D templates, such as those that currently exist for protein sequence templates, will help identify the functions of new protein structures as they are determined and pinpoint their functionally important regions.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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