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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Rapid degradation of AU-rich element (ARE) mRNAs is activated by ribosome transit and blocked by secondary structure at any position 5' to the ARE.</title><author><name sortKey="Curatola, A M" sort="Curatola, A M" uniqKey="Curatola A" first="A M" last="Curatola">A M Curatola</name></author><author><name sortKey="Nadal, M S" sort="Nadal, M S" uniqKey="Nadal M" first="M S" last="Nadal">M S Nadal</name></author><author><name sortKey="Schneider, R J" sort="Schneider, R J" uniqKey="Schneider R" first="R J" last="Schneider">R J Schneider</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">7565786</idno><idno type="pmc">230885</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC230885</idno><idno type="RBID">PMC:230885</idno><date when="1995">1995</date><idno type="wicri:Area/Pmc/Corpus">000085</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000085</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Rapid degradation of AU-rich element (ARE) mRNAs is activated by ribosome transit and blocked by secondary structure at any position 5' to the ARE.</title><author><name sortKey="Curatola, A M" sort="Curatola, A M" uniqKey="Curatola A" first="A M" last="Curatola">A M Curatola</name></author><author><name sortKey="Nadal, M S" sort="Nadal, M S" uniqKey="Nadal M" first="M S" last="Nadal">M S Nadal</name></author><author><name sortKey="Schneider, R J" sort="Schneider, R J" uniqKey="Schneider R" first="R J" last="Schneider">R J Schneider</name></author></analytic><series><title level="j">Molecular and Cellular Biology</title><idno type="ISSN">0270-7306</idno><idno type="eISSN">1098-5549</idno><imprint><date when="1995">1995</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The 3' noncoding region (NCR) AU-rich element (ARE) selectively confers rapid degradation on many mRNAs via a process requiring translation of the message. The role of cotranslation in destabilization of ARE mRNAs was examined by insertion of translation-blocking stable secondary structure at different sites in test mRNAs containing either the granulocyte-macrophage colony-stimulating factor (GM-CSF) ARE or a control sequence. A strong (-80 kcal/mol [1 kcal = 4.184 kJ]) but not a moderate (-30 kcal/mol) secondary structure prevented destabilization of mRNAs when inserted at any position upstream of the ARE, including in the 3' NCR. Surprisingly, a strong secondary structure did not block rapid mRNA decay when placed immediately downstream of the ARE. Studies are also presented showing that the turnover of mRNAs containing control or ARE sequences is not altered by insertion of long (1,000-nucleotide) intervening segments between the stop codon and the ARE or between the ARE and poly(A) tail. Characterization of ARE-containing mRNAs in polyadenylated and whole cytoplasmic RNA fractions failed to find evidence for decay intermediates degraded to the site of strong secondary structure from either the 5' or 3' end. From these and other data presented, this study demonstrates that complete translation of the coding region is essential for activation of rapid mRNA decay controlled by the GM-CSF ARE and that the structure of the 3' NCR can strongly influence activation. The results are consistent with activation of ARE-mediated decay by possible entry of translation-linked decay factors into the 3' NCR or translation-coupled changes in 3' NCR ribonucleoprotein structure or composition.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Mol Cell Biol</journal-id><journal-title>Molecular and Cellular Biology</journal-title><issn pub-type="ppub">0270-7306</issn><issn pub-type="epub">1098-5549</issn></journal-meta><article-meta><article-id pub-id-type="pmid">7565786</article-id><article-id pub-id-type="pmc">230885</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Rapid degradation of AU-rich element (ARE) mRNAs is activated by ribosome transit and blocked by secondary structure at any position 5' to the ARE.</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Curatola</surname><given-names>A M</given-names></name></contrib><contrib contrib-type="author"><name><surname>Nadal</surname><given-names>M S</given-names></name></contrib><contrib contrib-type="author"><name><surname>Schneider</surname><given-names>R J</given-names></name></contrib></contrib-group><aff>Department of Biochemistry, New York University Medical Center, New York 10016, USA.</aff><pub-date pub-type="ppub"><month>11</month><year>1995</year></pub-date><volume>15</volume><issue>11</issue><fpage>6331</fpage><lpage>6340</lpage><abstract><p>The 3' noncoding region (NCR) AU-rich element (ARE) selectively confers rapid degradation on many mRNAs via a process requiring translation of the message. The role of cotranslation in destabilization of ARE mRNAs was examined by insertion of translation-blocking stable secondary structure at different sites in test mRNAs containing either the granulocyte-macrophage colony-stimulating factor (GM-CSF) ARE or a control sequence. A strong (-80 kcal/mol [1 kcal = 4.184 kJ]) but not a moderate (-30 kcal/mol) secondary structure prevented destabilization of mRNAs when inserted at any position upstream of the ARE, including in the 3' NCR. Surprisingly, a strong secondary structure did not block rapid mRNA decay when placed immediately downstream of the ARE. Studies are also presented showing that the turnover of mRNAs containing control or ARE sequences is not altered by insertion of long (1,000-nucleotide) intervening segments between the stop codon and the ARE or between the ARE and poly(A) tail. Characterization of ARE-containing mRNAs in polyadenylated and whole cytoplasmic RNA fractions failed to find evidence for decay intermediates degraded to the site of strong secondary structure from either the 5' or 3' end. From these and other data presented, this study demonstrates that complete translation of the coding region is essential for activation of rapid mRNA decay controlled by the GM-CSF ARE and that the structure of the 3' NCR can strongly influence activation. The results are consistent with activation of ARE-mediated decay by possible entry of translation-linked decay factors into the 3' NCR or translation-coupled changes in 3' NCR ribonucleoprotein structure or composition.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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