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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Deficiency of 60 to 70<italic>S</italic> RNA in Murine Leukemia Virus Particles Assembled in Cells Treated with Actinomycin D</title><author><name sortKey="Levin, Judith G" sort="Levin, Judith G" uniqKey="Levin J" first="Judith G." last="Levin">Judith G. Levin</name></author><author><name sortKey="Grimley, Philip M" sort="Grimley, Philip M" uniqKey="Grimley P" first="Philip M." last="Grimley">Philip M. Grimley</name></author><author><name sortKey="Ramseur, Janet M" sort="Ramseur, Janet M" uniqKey="Ramseur J" first="Janet M." last="Ramseur">Janet M. Ramseur</name></author><author><name sortKey="Berezesky, Irene K" sort="Berezesky, Irene K" uniqKey="Berezesky I" first="Irene K." last="Berezesky">Irene K. Berezesky</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">4134468</idno><idno type="pmc">355489</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC355489</idno><idno type="RBID">PMC:355489</idno><date when="1974">1974</date><idno type="wicri:Area/Pmc/Corpus">000084</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000084</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Deficiency of 60 to 70<italic>S</italic> RNA in Murine Leukemia Virus Particles Assembled in Cells Treated with Actinomycin D</title><author><name sortKey="Levin, Judith G" sort="Levin, Judith G" uniqKey="Levin J" first="Judith G." last="Levin">Judith G. Levin</name></author><author><name sortKey="Grimley, Philip M" sort="Grimley, Philip M" uniqKey="Grimley P" first="Philip M." last="Grimley">Philip M. Grimley</name></author><author><name sortKey="Ramseur, Janet M" sort="Ramseur, Janet M" uniqKey="Ramseur J" first="Janet M." last="Ramseur">Janet M. Ramseur</name></author><author><name sortKey="Berezesky, Irene K" sort="Berezesky, Irene K" uniqKey="Berezesky I" first="Irene K." last="Berezesky">Irene K. Berezesky</name></author></analytic><series><title level="j">Journal of Virology</title><idno type="ISSN">0022-538X</idno><idno type="eISSN">1098-5514</idno><imprint><date when="1974">1974</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Production of particles with the ultrastructural appearance of C-type virions persisted for at least 6 h in actinomycin D-treated cells infected with murine leukemia virus. This phenomenon occurred despite severe inhibition of viral RNA synthesis. Virus particles present in a 6-h harvest sedimented in sucrose gradients with the buoyant density characteristic of RNA tumor viruses (1.16 g/cm<sup>3</sup>) and exhibited high levels of reverse transcriptase activity in response to the exogenous template polyriboadenylic acid·oligo deoxythymidylic acid in the range of untreated controls. However, RNase-sensitive endogenous activity was only ⅕ the level found in controls. This observation correlated with a marked reduction in infectivity. Kinetic studies on the appearance of labeled RNA in banded virions revealed that within the first hour after addition of actinomycin D, particles contained 60 to 70<italic>S</italic> RNA and two low-molecular-weight RNA species corresponding to 8 and 4<italic>S</italic> RNA. After approximately 1 h of incubation with actinomycin D, 60 to 70<italic>S</italic> RNA could not be detected and 4<italic>S</italic> RNA was the predominant species. These findings suggest that murine leukemia virus particles assembled in the presence of actinomycin D are deficient in 60 to 70<italic>S</italic> viral RNA.</p><sec sec-type="scanned-figures"><title>Images</title><fig id="F1"><graphic xlink:href="jvirol00247-0165-a" xlink:role="155"></graphic></fig><fig id="F2"><graphic xlink:href="jvirol00247-0165-b" xlink:role="155"></graphic></fig><fig id="F3"><graphic xlink:href="jvirol00247-0168-a" xlink:role="158"></graphic></fig></sec></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Virol</journal-id><journal-title>Journal of Virology</journal-title><issn pub-type="ppub">0022-538X</issn><issn pub-type="epub">1098-5514</issn></journal-meta><article-meta><article-id pub-id-type="pmid">4134468</article-id><article-id pub-id-type="pmc">355489</article-id><article-categories><subj-group subj-group-type="heading"><subject>Animal Viruses</subject></subj-group></article-categories><title-group><article-title>Deficiency of 60 to 70<italic>S</italic> RNA in Murine Leukemia Virus Particles Assembled in Cells Treated with Actinomycin D</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Levin</surname><given-names>Judith G.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Grimley</surname><given-names>Philip M.</given-names></name><xref ref-type="author-notes" rid="au1">1</xref></contrib><contrib contrib-type="author"><name><surname>Ramseur</surname><given-names>Janet M.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Berezesky</surname><given-names>Irene K.</given-names></name></contrib></contrib-group><aff id="af1"><label>a</label>Laboratory of Molecular Genetics, National Institute of Child Health and Human Development and Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014</aff><author-notes><fn id="au1"><label>1</label><p> Present address: Division of Laboratories and Research, New York State Department of Health, Albany, N.Y. 12201.</p></fn></author-notes><pub-date pub-type="ppub"><month>07</month><year>1974</year></pub-date><volume>14</volume><issue>1</issue><fpage>152</fpage><lpage>161</lpage><copyright-statement>Copyright © 1974 American Society for Microbiology</copyright-statement><abstract><p>Production of particles with the ultrastructural appearance of C-type virions persisted for at least 6 h in actinomycin D-treated cells infected with murine leukemia virus. This phenomenon occurred despite severe inhibition of viral RNA synthesis. Virus particles present in a 6-h harvest sedimented in sucrose gradients with the buoyant density characteristic of RNA tumor viruses (1.16 g/cm<sup>3</sup>) and exhibited high levels of reverse transcriptase activity in response to the exogenous template polyriboadenylic acid·oligo deoxythymidylic acid in the range of untreated controls. However, RNase-sensitive endogenous activity was only ⅕ the level found in controls. This observation correlated with a marked reduction in infectivity. Kinetic studies on the appearance of labeled RNA in banded virions revealed that within the first hour after addition of actinomycin D, particles contained 60 to 70<italic>S</italic> RNA and two low-molecular-weight RNA species corresponding to 8 and 4<italic>S</italic> RNA. After approximately 1 h of incubation with actinomycin D, 60 to 70<italic>S</italic> RNA could not be detected and 4<italic>S</italic> RNA was the predominant species. These findings suggest that murine leukemia virus particles assembled in the presence of actinomycin D are deficient in 60 to 70<italic>S</italic> viral RNA.</p><sec sec-type="scanned-figures"><title>Images</title><fig id="F1"><graphic xlink:href="jvirol00247-0165-a" xlink:role="155"></graphic></fig><fig id="F2"><graphic xlink:href="jvirol00247-0165-b" xlink:role="155"></graphic></fig><fig id="F3"><graphic xlink:href="jvirol00247-0168-a" xlink:role="158"></graphic></fig></sec></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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