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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Acetone and Butanol Production by <italic>Clostridium acetobutylicum</italic>
in a Synthetic Medium</title>
<author><name sortKey="Monot, Frederic" sort="Monot, Frederic" uniqKey="Monot F" first="Frédéric" last="Monot">Frédéric Monot</name>
</author>
<author><name sortKey="Martin, Jean Rene" sort="Martin, Jean Rene" uniqKey="Martin J" first="Jean-René" last="Martin">Jean-René Martin</name>
</author>
<author><name sortKey="Petitdemange, Henri" sort="Petitdemange, Henri" uniqKey="Petitdemange H" first="Henri" last="Petitdemange">Henri Petitdemange</name>
</author>
<author><name sortKey="Gay, Robert" sort="Gay, Robert" uniqKey="Gay R" first="Robert" last="Gay">Robert Gay</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">16346149</idno>
<idno type="pmc">242190</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC242190</idno>
<idno type="RBID">PMC:242190</idno>
<date when="1982">1982</date>
<idno type="wicri:Area/Pmc/Corpus">000047</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000047</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Acetone and Butanol Production by <italic>Clostridium acetobutylicum</italic>
in a Synthetic Medium</title>
<author><name sortKey="Monot, Frederic" sort="Monot, Frederic" uniqKey="Monot F" first="Frédéric" last="Monot">Frédéric Monot</name>
</author>
<author><name sortKey="Martin, Jean Rene" sort="Martin, Jean Rene" uniqKey="Martin J" first="Jean-René" last="Martin">Jean-René Martin</name>
</author>
<author><name sortKey="Petitdemange, Henri" sort="Petitdemange, Henri" uniqKey="Petitdemange H" first="Henri" last="Petitdemange">Henri Petitdemange</name>
</author>
<author><name sortKey="Gay, Robert" sort="Gay, Robert" uniqKey="Gay R" first="Robert" last="Gay">Robert Gay</name>
</author>
</analytic>
<series><title level="j">Applied and Environmental Microbiology</title>
<idno type="ISSN">0099-2240</idno>
<idno type="eISSN">1098-5336</idno>
<imprint><date when="1982">1982</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>The effect of the component concentrations of a synthetic medium on acetone and butanol fermentation by <italic>Clostridium acetobutylicum</italic>
ATCC 824 was investigated. Cell growth was dependent on the presence of Mg, Fe, and K in the medium. Mg and Mn had deleterious effects when in excess. Ammonium acetate in excess caused acid fermentation. The metabolism was composed of two phases: an acid phase and a solvent one. Low concentrations of glucose allowed the first phase only. The theoretical ratio of the conversion of glucose to solvents, which was 28 to 33%, was obtained with the following medium: MgSO<sub>4</sub>
, 50 to 200 mg/liter; MnSO<sub>4</sub>
, 0 to 20 mg/liter; KCl, 0.015 to 8 g/liter (an equivalent concentration of K<sup>+</sup>
was supplied in the form of KH<sub>2</sub>
PO<sub>4</sub>
and K<sub>2</sub>
HPO<sub>4</sub>
); FeSO<sub>4</sub>
, 1 to 50 mg/liter; ammonium acetate, 1.1 to 2.2 g/liter; <italic>para</italic>
-aminobenzoic acid, 1 mg/liter; biotin, 0.01 mg/liter; glucose, 20 to 60 g/liter.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Appl Environ Microbiol</journal-id>
<journal-title>Applied and Environmental Microbiology</journal-title>
<issn pub-type="ppub">0099-2240</issn>
<issn pub-type="epub">1098-5336</issn>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">16346149</article-id>
<article-id pub-id-type="pmc">242190</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Metabolism, Growth, and Industrial Microbiology</subject>
</subj-group>
</article-categories>
<title-group><article-title>Acetone and Butanol Production by <italic>Clostridium acetobutylicum</italic>
in a Synthetic Medium</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Monot</surname>
<given-names>Frédéric</given-names>
</name>
<xref ref-type="aff">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Martin</surname>
<given-names>Jean-René</given-names>
</name>
<xref ref-type="aff">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Petitdemange</surname>
<given-names>Henri</given-names>
</name>
<xref ref-type="aff">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Gay</surname>
<given-names>Robert</given-names>
</name>
<xref ref-type="aff">2</xref>
</contrib>
</contrib-group>
<aff id="af1"><label>1</label>
Laboratoire des Sciences du Génie Chimique, CNRS-ENSIC, 54042 Nancy, Cedex, France, and Université de Nancy I, Laboratoire de Chimie Biologique I, 54037 Nancy, Cedex, France</aff>
<pub-date pub-type="ppub"><month>12</month>
<year>1982</year>
</pub-date>
<volume>44</volume>
<issue>6</issue>
<fpage>1318</fpage>
<lpage>1324</lpage>
<copyright-statement>Copyright © 1982, American Society for Microbiology</copyright-statement>
<abstract><p>The effect of the component concentrations of a synthetic medium on acetone and butanol fermentation by <italic>Clostridium acetobutylicum</italic>
ATCC 824 was investigated. Cell growth was dependent on the presence of Mg, Fe, and K in the medium. Mg and Mn had deleterious effects when in excess. Ammonium acetate in excess caused acid fermentation. The metabolism was composed of two phases: an acid phase and a solvent one. Low concentrations of glucose allowed the first phase only. The theoretical ratio of the conversion of glucose to solvents, which was 28 to 33%, was obtained with the following medium: MgSO<sub>4</sub>
, 50 to 200 mg/liter; MnSO<sub>4</sub>
, 0 to 20 mg/liter; KCl, 0.015 to 8 g/liter (an equivalent concentration of K<sup>+</sup>
was supplied in the form of KH<sub>2</sub>
PO<sub>4</sub>
and K<sub>2</sub>
HPO<sub>4</sub>
); FeSO<sub>4</sub>
, 1 to 50 mg/liter; ammonium acetate, 1.1 to 2.2 g/liter; <italic>para</italic>
-aminobenzoic acid, 1 mg/liter; biotin, 0.01 mg/liter; glucose, 20 to 60 g/liter.</p>
</abstract>
</article-meta>
</front>
</pmc>
</record>
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