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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Acetone and Butanol Production by <italic>Clostridium acetobutylicum</italic> in a Synthetic Medium</title><author><name sortKey="Monot, Frederic" sort="Monot, Frederic" uniqKey="Monot F" first="Frédéric" last="Monot">Frédéric Monot</name></author><author><name sortKey="Martin, Jean Rene" sort="Martin, Jean Rene" uniqKey="Martin J" first="Jean-René" last="Martin">Jean-René Martin</name></author><author><name sortKey="Petitdemange, Henri" sort="Petitdemange, Henri" uniqKey="Petitdemange H" first="Henri" last="Petitdemange">Henri Petitdemange</name></author><author><name sortKey="Gay, Robert" sort="Gay, Robert" uniqKey="Gay R" first="Robert" last="Gay">Robert Gay</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">16346149</idno><idno type="pmc">242190</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC242190</idno><idno type="RBID">PMC:242190</idno><date when="1982">1982</date><idno type="wicri:Area/Pmc/Corpus">000047</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000047</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Acetone and Butanol Production by <italic>Clostridium acetobutylicum</italic> in a Synthetic Medium</title><author><name sortKey="Monot, Frederic" sort="Monot, Frederic" uniqKey="Monot F" first="Frédéric" last="Monot">Frédéric Monot</name></author><author><name sortKey="Martin, Jean Rene" sort="Martin, Jean Rene" uniqKey="Martin J" first="Jean-René" last="Martin">Jean-René Martin</name></author><author><name sortKey="Petitdemange, Henri" sort="Petitdemange, Henri" uniqKey="Petitdemange H" first="Henri" last="Petitdemange">Henri Petitdemange</name></author><author><name sortKey="Gay, Robert" sort="Gay, Robert" uniqKey="Gay R" first="Robert" last="Gay">Robert Gay</name></author></analytic><series><title level="j">Applied and Environmental Microbiology</title><idno type="ISSN">0099-2240</idno><idno type="eISSN">1098-5336</idno><imprint><date when="1982">1982</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The effect of the component concentrations of a synthetic medium on acetone and butanol fermentation by <italic>Clostridium acetobutylicum</italic> ATCC 824 was investigated. Cell growth was dependent on the presence of Mg, Fe, and K in the medium. Mg and Mn had deleterious effects when in excess. Ammonium acetate in excess caused acid fermentation. The metabolism was composed of two phases: an acid phase and a solvent one. Low concentrations of glucose allowed the first phase only. The theoretical ratio of the conversion of glucose to solvents, which was 28 to 33%, was obtained with the following medium: MgSO<sub>4</sub>, 50 to 200 mg/liter; MnSO<sub>4</sub>, 0 to 20 mg/liter; KCl, 0.015 to 8 g/liter (an equivalent concentration of K<sup>+</sup> was supplied in the form of KH<sub>2</sub>PO<sub>4</sub> and K<sub>2</sub>HPO<sub>4</sub>); FeSO<sub>4</sub>, 1 to 50 mg/liter; ammonium acetate, 1.1 to 2.2 g/liter; <italic>para</italic>-aminobenzoic acid, 1 mg/liter; biotin, 0.01 mg/liter; glucose, 20 to 60 g/liter.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Appl Environ Microbiol</journal-id><journal-title>Applied and Environmental Microbiology</journal-title><issn pub-type="ppub">0099-2240</issn><issn pub-type="epub">1098-5336</issn></journal-meta><article-meta><article-id pub-id-type="pmid">16346149</article-id><article-id pub-id-type="pmc">242190</article-id><article-categories><subj-group subj-group-type="heading"><subject>Metabolism, Growth, and Industrial Microbiology</subject></subj-group></article-categories><title-group><article-title>Acetone and Butanol Production by <italic>Clostridium acetobutylicum</italic> in a Synthetic Medium</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Monot</surname><given-names>Frédéric</given-names></name><xref ref-type="aff">1</xref></contrib><contrib contrib-type="author"><name><surname>Martin</surname><given-names>Jean-René</given-names></name><xref ref-type="aff">2</xref></contrib><contrib contrib-type="author"><name><surname>Petitdemange</surname><given-names>Henri</given-names></name><xref ref-type="aff">2</xref></contrib><contrib contrib-type="author"><name><surname>Gay</surname><given-names>Robert</given-names></name><xref ref-type="aff">2</xref></contrib></contrib-group><aff id="af1"><label>1</label>Laboratoire des Sciences du Génie Chimique, CNRS-ENSIC, 54042 Nancy, Cedex, France, and Université de Nancy I, Laboratoire de Chimie Biologique I, 54037 Nancy, Cedex, France</aff><pub-date pub-type="ppub"><month>12</month><year>1982</year></pub-date><volume>44</volume><issue>6</issue><fpage>1318</fpage><lpage>1324</lpage><copyright-statement>Copyright © 1982, American Society for Microbiology</copyright-statement><abstract><p>The effect of the component concentrations of a synthetic medium on acetone and butanol fermentation by <italic>Clostridium acetobutylicum</italic> ATCC 824 was investigated. Cell growth was dependent on the presence of Mg, Fe, and K in the medium. Mg and Mn had deleterious effects when in excess. Ammonium acetate in excess caused acid fermentation. The metabolism was composed of two phases: an acid phase and a solvent one. Low concentrations of glucose allowed the first phase only. The theoretical ratio of the conversion of glucose to solvents, which was 28 to 33%, was obtained with the following medium: MgSO<sub>4</sub>, 50 to 200 mg/liter; MnSO<sub>4</sub>, 0 to 20 mg/liter; KCl, 0.015 to 8 g/liter (an equivalent concentration of K<sup>+</sup> was supplied in the form of KH<sub>2</sub>PO<sub>4</sub> and K<sub>2</sub>HPO<sub>4</sub>); FeSO<sub>4</sub>, 1 to 50 mg/liter; ammonium acetate, 1.1 to 2.2 g/liter; <italic>para</italic>-aminobenzoic acid, 1 mg/liter; biotin, 0.01 mg/liter; glucose, 20 to 60 g/liter.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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