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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Combination of yeast hydrolysates to improve CHO cell growth and IgG production</title><author><name sortKey="Mosser, Mathilde" sort="Mosser, Mathilde" uniqKey="Mosser M" first="Mathilde" last="Mosser">Mathilde Mosser</name><affiliation><nlm:aff id="Aff1">CNRS, Laboratoire Réactions et Génie des Procédés, UPR- 3349, 2 avenue de la forêt de Haye, 54505 Vandœuvre-lès-Nancy, France</nlm:aff></affiliation><affiliation><nlm:aff id="Aff2">ENSAIA, Laboratoire Réactions et Génie des Procédés, Université de Lorraine, 2 avenue de la forêt de Haye, 54505 Vandœuvre-lès-Nancy, France</nlm:aff></affiliation><affiliation><nlm:aff id="Aff3">Bio Springer, 103 rue Jean Jaurès, 94704 Maisons-Alfort, France</nlm:aff></affiliation></author><author><name sortKey="Chevalot, Isabelle" sort="Chevalot, Isabelle" uniqKey="Chevalot I" first="Isabelle" last="Chevalot">Isabelle Chevalot</name><affiliation><nlm:aff id="Aff1">CNRS, Laboratoire Réactions et Génie des Procédés, UPR- 3349, 2 avenue de la forêt de Haye, 54505 Vandœuvre-lès-Nancy, France</nlm:aff></affiliation><affiliation><nlm:aff id="Aff2">ENSAIA, Laboratoire Réactions et Génie des Procédés, Université de Lorraine, 2 avenue de la forêt de Haye, 54505 Vandœuvre-lès-Nancy, France</nlm:aff></affiliation><affiliation><nlm:aff id="Aff4">LRGP, UPR-CNRS 3349, Université de Lorraine, 2, avenue de la forêt de Haye, 54505 Vandœuvre-lès-Nancy, France</nlm:aff></affiliation></author><author><name sortKey="Olmos, Eric" sort="Olmos, Eric" uniqKey="Olmos E" first="Eric" last="Olmos">Eric Olmos</name><affiliation><nlm:aff id="Aff1">CNRS, Laboratoire Réactions et Génie des Procédés, UPR- 3349, 2 avenue de la forêt de Haye, 54505 Vandœuvre-lès-Nancy, France</nlm:aff></affiliation><affiliation><nlm:aff id="Aff2">ENSAIA, Laboratoire Réactions et Génie des Procédés, Université de Lorraine, 2 avenue de la forêt de Haye, 54505 Vandœuvre-lès-Nancy, France</nlm:aff></affiliation></author><author><name sortKey="Blanchard, Fabrice" sort="Blanchard, Fabrice" uniqKey="Blanchard F" first="Fabrice" last="Blanchard">Fabrice Blanchard</name><affiliation><nlm:aff id="Aff1">CNRS, Laboratoire Réactions et Génie des Procédés, UPR- 3349, 2 avenue de la forêt de Haye, 54505 Vandœuvre-lès-Nancy, France</nlm:aff></affiliation><affiliation><nlm:aff id="Aff2">ENSAIA, Laboratoire Réactions et Génie des Procédés, Université de Lorraine, 2 avenue de la forêt de Haye, 54505 Vandœuvre-lès-Nancy, France</nlm:aff></affiliation></author><author><name sortKey="Kapel, Romain" sort="Kapel, Romain" uniqKey="Kapel R" first="Romain" last="Kapel">Romain Kapel</name><affiliation><nlm:aff id="Aff1">CNRS, Laboratoire Réactions et Génie des Procédés, UPR- 3349, 2 avenue de la forêt de Haye, 54505 Vandœuvre-lès-Nancy, France</nlm:aff></affiliation><affiliation><nlm:aff id="Aff2">ENSAIA, Laboratoire Réactions et Génie des Procédés, Université de Lorraine, 2 avenue de la forêt de Haye, 54505 Vandœuvre-lès-Nancy, France</nlm:aff></affiliation></author><author><name sortKey="Oriol, Eric" sort="Oriol, Eric" uniqKey="Oriol E" first="Eric" last="Oriol">Eric Oriol</name><affiliation><nlm:aff id="Aff3">Bio Springer, 103 rue Jean Jaurès, 94704 Maisons-Alfort, France</nlm:aff></affiliation></author><author><name sortKey="Marc, Ivan" sort="Marc, Ivan" uniqKey="Marc I" first="Ivan" last="Marc">Ivan Marc</name><affiliation><nlm:aff id="Aff1">CNRS, Laboratoire Réactions et Génie des Procédés, UPR- 3349, 2 avenue de la forêt de Haye, 54505 Vandœuvre-lès-Nancy, France</nlm:aff></affiliation><affiliation><nlm:aff id="Aff2">ENSAIA, Laboratoire Réactions et Génie des Procédés, Université de Lorraine, 2 avenue de la forêt de Haye, 54505 Vandœuvre-lès-Nancy, France</nlm:aff></affiliation></author><author><name sortKey="Marc, Annie" sort="Marc, Annie" uniqKey="Marc A" first="Annie" last="Marc">Annie Marc</name><affiliation><nlm:aff id="Aff1">CNRS, Laboratoire Réactions et Génie des Procédés, UPR- 3349, 2 avenue de la forêt de Haye, 54505 Vandœuvre-lès-Nancy, France</nlm:aff></affiliation><affiliation><nlm:aff id="Aff2">ENSAIA, Laboratoire Réactions et Génie des Procédés, Université de Lorraine, 2 avenue de la forêt de Haye, 54505 Vandœuvre-lès-Nancy, France</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">23239488</idno><idno type="pmc">3720966</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3720966</idno><idno type="RBID">PMC:3720966</idno><idno type="doi">10.1007/s10616-012-9519-1</idno><date when="2012">2012</date><idno type="wicri:Area/Pmc/Corpus">000020</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000020</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Combination of yeast hydrolysates to improve CHO cell growth and IgG production</title><author><name sortKey="Mosser, Mathilde" sort="Mosser, Mathilde" uniqKey="Mosser M" first="Mathilde" last="Mosser">Mathilde Mosser</name><affiliation><nlm:aff id="Aff1">CNRS, Laboratoire Réactions et Génie des Procédés, UPR- 3349, 2 avenue de la forêt de Haye, 54505 Vandœuvre-lès-Nancy, France</nlm:aff></affiliation><affiliation><nlm:aff id="Aff2">ENSAIA, Laboratoire Réactions et Génie des Procédés, Université de Lorraine, 2 avenue de la forêt de Haye, 54505 Vandœuvre-lès-Nancy, France</nlm:aff></affiliation><affiliation><nlm:aff id="Aff3">Bio Springer, 103 rue Jean Jaurès, 94704 Maisons-Alfort, France</nlm:aff></affiliation></author><author><name sortKey="Chevalot, Isabelle" sort="Chevalot, Isabelle" uniqKey="Chevalot I" first="Isabelle" last="Chevalot">Isabelle Chevalot</name><affiliation><nlm:aff id="Aff1">CNRS, Laboratoire Réactions et Génie des Procédés, UPR- 3349, 2 avenue de la forêt de Haye, 54505 Vandœuvre-lès-Nancy, France</nlm:aff></affiliation><affiliation><nlm:aff id="Aff2">ENSAIA, Laboratoire Réactions et Génie des Procédés, Université de Lorraine, 2 avenue de la forêt de Haye, 54505 Vandœuvre-lès-Nancy, France</nlm:aff></affiliation><affiliation><nlm:aff id="Aff4">LRGP, UPR-CNRS 3349, Université de Lorraine, 2, avenue de la forêt de Haye, 54505 Vandœuvre-lès-Nancy, France</nlm:aff></affiliation></author><author><name sortKey="Olmos, Eric" sort="Olmos, Eric" uniqKey="Olmos E" first="Eric" last="Olmos">Eric Olmos</name><affiliation><nlm:aff id="Aff1">CNRS, Laboratoire Réactions et Génie des Procédés, UPR- 3349, 2 avenue de la forêt de Haye, 54505 Vandœuvre-lès-Nancy, France</nlm:aff></affiliation><affiliation><nlm:aff id="Aff2">ENSAIA, Laboratoire Réactions et Génie des Procédés, Université de Lorraine, 2 avenue de la forêt de Haye, 54505 Vandœuvre-lès-Nancy, France</nlm:aff></affiliation></author><author><name sortKey="Blanchard, Fabrice" sort="Blanchard, Fabrice" uniqKey="Blanchard F" first="Fabrice" last="Blanchard">Fabrice Blanchard</name><affiliation><nlm:aff id="Aff1">CNRS, Laboratoire Réactions et Génie des Procédés, UPR- 3349, 2 avenue de la forêt de Haye, 54505 Vandœuvre-lès-Nancy, France</nlm:aff></affiliation><affiliation><nlm:aff id="Aff2">ENSAIA, Laboratoire Réactions et Génie des Procédés, Université de Lorraine, 2 avenue de la forêt de Haye, 54505 Vandœuvre-lès-Nancy, France</nlm:aff></affiliation></author><author><name sortKey="Kapel, Romain" sort="Kapel, Romain" uniqKey="Kapel R" first="Romain" last="Kapel">Romain Kapel</name><affiliation><nlm:aff id="Aff1">CNRS, Laboratoire Réactions et Génie des Procédés, UPR- 3349, 2 avenue de la forêt de Haye, 54505 Vandœuvre-lès-Nancy, France</nlm:aff></affiliation><affiliation><nlm:aff id="Aff2">ENSAIA, Laboratoire Réactions et Génie des Procédés, Université de Lorraine, 2 avenue de la forêt de Haye, 54505 Vandœuvre-lès-Nancy, France</nlm:aff></affiliation></author><author><name sortKey="Oriol, Eric" sort="Oriol, Eric" uniqKey="Oriol E" first="Eric" last="Oriol">Eric Oriol</name><affiliation><nlm:aff id="Aff3">Bio Springer, 103 rue Jean Jaurès, 94704 Maisons-Alfort, France</nlm:aff></affiliation></author><author><name sortKey="Marc, Ivan" sort="Marc, Ivan" uniqKey="Marc I" first="Ivan" last="Marc">Ivan Marc</name><affiliation><nlm:aff id="Aff1">CNRS, Laboratoire Réactions et Génie des Procédés, UPR- 3349, 2 avenue de la forêt de Haye, 54505 Vandœuvre-lès-Nancy, France</nlm:aff></affiliation><affiliation><nlm:aff id="Aff2">ENSAIA, Laboratoire Réactions et Génie des Procédés, Université de Lorraine, 2 avenue de la forêt de Haye, 54505 Vandœuvre-lès-Nancy, France</nlm:aff></affiliation></author><author><name sortKey="Marc, Annie" sort="Marc, Annie" uniqKey="Marc A" first="Annie" last="Marc">Annie Marc</name><affiliation><nlm:aff id="Aff1">CNRS, Laboratoire Réactions et Génie des Procédés, UPR- 3349, 2 avenue de la forêt de Haye, 54505 Vandœuvre-lès-Nancy, France</nlm:aff></affiliation><affiliation><nlm:aff id="Aff2">ENSAIA, Laboratoire Réactions et Génie des Procédés, Université de Lorraine, 2 avenue de la forêt de Haye, 54505 Vandœuvre-lès-Nancy, France</nlm:aff></affiliation></author></analytic><series><title level="j">Cytotechnology</title><idno type="ISSN">0920-9069</idno><idno type="eISSN">1573-0778</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Many studies underlined the great benefits of hydrolysates used as additives in animal free media on cell culture performances. However, to precisely define hydrolysate supplementation strategies, a deeper understanding of their effect on cell growth and protein production is required. In the present study, the effect of addition of one yeast extract (YE) and two yeast peptones (named YP.A and YP.B) in a chemically defined medium was first assessed on cell culture performances. Interestingly, specific effects were found depending on the degree of degradation of yeast hydrolysates. The YE at 1 g L<sup>−1</sup> increased the maximal cell density by 70 %, while a mixture of YE (1 g L<sup>−1</sup>) and YP.A (4 g L<sup>−1</sup>) increased IgG production by 180 %. These conditions were then evaluated on the CHO cell kinetics all over cultures. Hydrolysates extended the cell growth phase in Erlenmeyer flask and increased the maximal growth rate in bioreactor up to 20 %. Cell growth stimulation induced by hydrolysates addition was linked with energetic metabolism improvement suggesting that they promote oxidative pathway. Furthermore, hydrolysates provided an additional source of substrate that supported cell growth despite glutamine limitation.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Cytotechnology</journal-id><journal-id journal-id-type="iso-abbrev">Cytotechnology</journal-id><journal-title-group><journal-title>Cytotechnology</journal-title></journal-title-group><issn pub-type="ppub">0920-9069</issn><issn pub-type="epub">1573-0778</issn><publisher><publisher-name>Springer Netherlands</publisher-name><publisher-loc>Dordrecht</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">23239488</article-id><article-id pub-id-type="pmc">3720966</article-id><article-id pub-id-type="publisher-id">9519</article-id><article-id pub-id-type="doi">10.1007/s10616-012-9519-1</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Research</subject></subj-group></article-categories><title-group><article-title>Combination of yeast hydrolysates to improve CHO cell growth and IgG production</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Mosser</surname><given-names>Mathilde</given-names></name><xref ref-type="aff" rid="Aff1"></xref><xref ref-type="aff" rid="Aff2"></xref><xref ref-type="aff" rid="Aff3"></xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Chevalot</surname><given-names>Isabelle</given-names></name><address><email>Isabelle.Chevalot@ensaia.inpl-nancy.fr</email></address><xref ref-type="aff" rid="Aff1"></xref><xref ref-type="aff" rid="Aff2"></xref><xref ref-type="aff" rid="Aff4"></xref></contrib><contrib contrib-type="author"><name><surname>Olmos</surname><given-names>Eric</given-names></name><xref ref-type="aff" rid="Aff1"></xref><xref ref-type="aff" rid="Aff2"></xref></contrib><contrib contrib-type="author"><name><surname>Blanchard</surname><given-names>Fabrice</given-names></name><xref ref-type="aff" rid="Aff1"></xref><xref ref-type="aff" rid="Aff2"></xref></contrib><contrib contrib-type="author"><name><surname>Kapel</surname><given-names>Romain</given-names></name><xref ref-type="aff" rid="Aff1"></xref><xref ref-type="aff" rid="Aff2"></xref></contrib><contrib contrib-type="author"><name><surname>Oriol</surname><given-names>Eric</given-names></name><xref ref-type="aff" rid="Aff3"></xref></contrib><contrib contrib-type="author"><name><surname>Marc</surname><given-names>Ivan</given-names></name><xref ref-type="aff" rid="Aff1"></xref><xref ref-type="aff" rid="Aff2"></xref></contrib><contrib contrib-type="author"><name><surname>Marc</surname><given-names>Annie</given-names></name><xref ref-type="aff" rid="Aff1"></xref><xref ref-type="aff" rid="Aff2"></xref></contrib><aff id="Aff1"><label></label>CNRS, Laboratoire Réactions et Génie des Procédés, UPR- 3349, 2 avenue de la forêt de Haye, 54505 Vandœuvre-lès-Nancy, France</aff><aff id="Aff2"><label></label>ENSAIA, Laboratoire Réactions et Génie des Procédés, Université de Lorraine, 2 avenue de la forêt de Haye, 54505 Vandœuvre-lès-Nancy, France</aff><aff id="Aff3"><label></label>Bio Springer, 103 rue Jean Jaurès, 94704 Maisons-Alfort, France</aff><aff id="Aff4"><label></label>LRGP, UPR-CNRS 3349, Université de Lorraine, 2, avenue de la forêt de Haye, 54505 Vandœuvre-lès-Nancy, France</aff></contrib-group><pub-date pub-type="epub"><day>14</day><month>12</month><year>2012</year></pub-date><pub-date pub-type="ppub"><month>8</month><year>2013</year></pub-date><volume>65</volume><issue>4</issue><fpage>629</fpage><lpage>641</lpage><history><date date-type="received"><day>7</day><month>6</month><year>2012</year></date><date date-type="accepted"><day>18</day><month>11</month><year>2012</year></date></history><permissions><copyright-statement>© Springer Science+Business Media Dordrecht 2012</copyright-statement></permissions><abstract id="Abs1"><p>Many studies underlined the great benefits of hydrolysates used as additives in animal free media on cell culture performances. However, to precisely define hydrolysate supplementation strategies, a deeper understanding of their effect on cell growth and protein production is required. In the present study, the effect of addition of one yeast extract (YE) and two yeast peptones (named YP.A and YP.B) in a chemically defined medium was first assessed on cell culture performances. Interestingly, specific effects were found depending on the degree of degradation of yeast hydrolysates. The YE at 1 g L<sup>−1</sup> increased the maximal cell density by 70 %, while a mixture of YE (1 g L<sup>−1</sup>) and YP.A (4 g L<sup>−1</sup>) increased IgG production by 180 %. These conditions were then evaluated on the CHO cell kinetics all over cultures. Hydrolysates extended the cell growth phase in Erlenmeyer flask and increased the maximal growth rate in bioreactor up to 20 %. Cell growth stimulation induced by hydrolysates addition was linked with energetic metabolism improvement suggesting that they promote oxidative pathway. Furthermore, hydrolysates provided an additional source of substrate that supported cell growth despite glutamine limitation.</p></abstract><kwd-group xml:lang="en"><title>Keywords</title><kwd>Yeast extract</kwd><kwd>Yeast peptone</kwd><kwd>CHO cell culture</kwd><kwd>IgG recombinant protein</kwd><kwd>Bioreactor</kwd><kwd>Kinetic studies</kwd></kwd-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© Springer Science+Business Media Dordrecht 2013</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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