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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Characterization of Vero cell growth and death in bioreactor with serum-containing and serum-free media</title><author><name sortKey="Quesney, Sebastien" sort="Quesney, Sebastien" uniqKey="Quesney S" first="Sébastien" last="Quesney">Sébastien Quesney</name><affiliation><nlm:aff id="Aff1">Development Department, Aventis Pasteur, Campus Mérieux, 1541 Avenue Marcel Mérieux, F-69280 Marcy L'étoile, France</nlm:aff></affiliation></author><author><name sortKey="Marvel, Jacqueline" sort="Marvel, Jacqueline" uniqKey="Marvel J" first="Jacqueline" last="Marvel">Jacqueline Marvel</name><affiliation><nlm:aff id="Aff2">Centre Européen de Recherche en Virologie et Immunologie, INSERM, unité 503, 21 Avenue Tony Garnier, F-69365 Lyon, France</nlm:aff></affiliation></author><author><name sortKey="Marc, Annie" sort="Marc, Annie" uniqKey="Marc A" first="Annie" last="Marc">Annie Marc</name><affiliation><nlm:aff id="Aff3">Laboratoire des Sciences du Génie Chimique, CNRS, UPR 6811, ENSIC, 1 Rue Granville, F-54001 Nancy, France</nlm:aff></affiliation></author><author><name sortKey="Gerdil, Catherine" sort="Gerdil, Catherine" uniqKey="Gerdil C" first="Catherine" last="Gerdil">Catherine Gerdil</name><affiliation><nlm:aff id="Aff4">Development Department, Aventis Pasteur, Campus Mérieux, 1541 Avenue Marcel Mérieux, F-69280 Marcy L'étoile, France</nlm:aff></affiliation></author><author><name sortKey="Meignier, Bernard" sort="Meignier, Bernard" uniqKey="Meignier B" first="Bernard" last="Meignier">Bernard Meignier</name><affiliation><nlm:aff id="Aff1">Development Department, Aventis Pasteur, Campus Mérieux, 1541 Avenue Marcel Mérieux, F-69280 Marcy L'étoile, France</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">19003288</idno><idno type="pmc">3449459</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3449459</idno><idno type="RBID">PMC:3449459</idno><idno type="doi">10.1023/A:1017589526145</idno><date when="2001">2001</date><idno type="wicri:Area/Pmc/Corpus">000018</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000018</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Characterization of Vero cell growth and death in bioreactor with serum-containing and serum-free media</title><author><name sortKey="Quesney, Sebastien" sort="Quesney, Sebastien" uniqKey="Quesney S" first="Sébastien" last="Quesney">Sébastien Quesney</name><affiliation><nlm:aff id="Aff1">Development Department, Aventis Pasteur, Campus Mérieux, 1541 Avenue Marcel Mérieux, F-69280 Marcy L'étoile, France</nlm:aff></affiliation></author><author><name sortKey="Marvel, Jacqueline" sort="Marvel, Jacqueline" uniqKey="Marvel J" first="Jacqueline" last="Marvel">Jacqueline Marvel</name><affiliation><nlm:aff id="Aff2">Centre Européen de Recherche en Virologie et Immunologie, INSERM, unité 503, 21 Avenue Tony Garnier, F-69365 Lyon, France</nlm:aff></affiliation></author><author><name sortKey="Marc, Annie" sort="Marc, Annie" uniqKey="Marc A" first="Annie" last="Marc">Annie Marc</name><affiliation><nlm:aff id="Aff3">Laboratoire des Sciences du Génie Chimique, CNRS, UPR 6811, ENSIC, 1 Rue Granville, F-54001 Nancy, France</nlm:aff></affiliation></author><author><name sortKey="Gerdil, Catherine" sort="Gerdil, Catherine" uniqKey="Gerdil C" first="Catherine" last="Gerdil">Catherine Gerdil</name><affiliation><nlm:aff id="Aff4">Development Department, Aventis Pasteur, Campus Mérieux, 1541 Avenue Marcel Mérieux, F-69280 Marcy L'étoile, France</nlm:aff></affiliation></author><author><name sortKey="Meignier, Bernard" sort="Meignier, Bernard" uniqKey="Meignier B" first="Bernard" last="Meignier">Bernard Meignier</name><affiliation><nlm:aff id="Aff1">Development Department, Aventis Pasteur, Campus Mérieux, 1541 Avenue Marcel Mérieux, F-69280 Marcy L'étoile, France</nlm:aff></affiliation></author></analytic><series><title level="j">Cytotechnology</title><idno type="ISSN">0920-9069</idno><idno type="eISSN">1573-0778</idno><imprint><date when="2001">2001</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The density of viable cells in a culture results from a balance between cell proliferation and cell death. The aim of this study was to characterize and compare these two phenomena in Vero cell cultures in one serum containing medium (ScA) and one serum free medium (SfB) in bioreactors. Cell growth was evaluated by cell counting(after crystal violet staining) and cell cycle analysis. Necrosis and apoptosis were characterized and quantified by measuring the release of LDH, trypan blue exclusion,annex in V-FITC/PI staining and TUNEL assay. ScA supported a higher maximal viable-cell density(2.3 × 10<sup>6</sup> vs. 1.8 × 10<sup>6</sup> cells ml<sup>-1</sup>). However, cell cycle analysis showed that cell division was more active in SfB than in ScA. LDH release in the supernatant increased much earlier in SfB than in ScA (one vs. five days), but trypan blue counts showed no apparent difference in the viability of the cultures. Apoptosis, evidenced by annexin V-FITC/PI staining, could be detected in the population of suspension cells detached from microcarriers, but not among adherent cells; positivity of the TUNEL assay occurred later than that of the annexin V-FITC/PI staining. Our data indicate that the lower cell yield in SfB,compared with that in ScA, results from a higher cell death rate. Apparently, cells die from apoptosis followed by secondary necrosis.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Cytotechnology</journal-id><journal-id journal-id-type="iso-abbrev">Cytotechnology</journal-id><journal-title-group><journal-title>Cytotechnology</journal-title></journal-title-group><issn pub-type="ppub">0920-9069</issn><issn pub-type="epub">1573-0778</issn><publisher><publisher-name>Kluwer Academic Publishers</publisher-name><publisher-loc>Dordrecht</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">19003288</article-id><article-id pub-id-type="pmc">3449459</article-id><article-id pub-id-type="publisher-id">321530</article-id><article-id pub-id-type="doi">10.1023/A:1017589526145</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Characterization of Vero cell growth and death in bioreactor with serum-containing and serum-free media</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Quesney</surname><given-names>Sébastien</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Marvel</surname><given-names>Jacqueline</given-names></name><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Marc</surname><given-names>Annie</given-names></name><xref ref-type="aff" rid="Aff3">3</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Gerdil</surname><given-names>Catherine</given-names></name><address><email>Catherine.Gerdil@aventis.com</email></address><xref ref-type="aff" rid="Aff4">4</xref></contrib><contrib contrib-type="author"><name><surname>Meignier</surname><given-names>Bernard</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><aff id="Aff1"><label>1</label>Development Department, Aventis Pasteur, Campus Mérieux, 1541 Avenue Marcel Mérieux, F-69280 Marcy L'étoile, France</aff><aff id="Aff2"><label>2</label>Centre Européen de Recherche en Virologie et Immunologie, INSERM, unité 503, 21 Avenue Tony Garnier, F-69365 Lyon, France</aff><aff id="Aff3"><label>3</label>Laboratoire des Sciences du Génie Chimique, CNRS, UPR 6811, ENSIC, 1 Rue Granville, F-54001 Nancy, France</aff><aff id="Aff4"><label>4</label>Development Department, Aventis Pasteur, Campus Mérieux, 1541 Avenue Marcel Mérieux, F-69280 Marcy L'étoile, France</aff></contrib-group><pub-date pub-type="ppub"><month>3</month><year>2001</year></pub-date><volume>35</volume><issue>2</issue><fpage>115</fpage><lpage>125</lpage><permissions><copyright-statement>© Kluwer Academic Publishers 2001</copyright-statement></permissions><abstract id="Abs1"><p>The density of viable cells in a culture results from a balance between cell proliferation and cell death. The aim of this study was to characterize and compare these two phenomena in Vero cell cultures in one serum containing medium (ScA) and one serum free medium (SfB) in bioreactors. Cell growth was evaluated by cell counting(after crystal violet staining) and cell cycle analysis. Necrosis and apoptosis were characterized and quantified by measuring the release of LDH, trypan blue exclusion,annex in V-FITC/PI staining and TUNEL assay. ScA supported a higher maximal viable-cell density(2.3 × 10<sup>6</sup> vs. 1.8 × 10<sup>6</sup> cells ml<sup>-1</sup>). However, cell cycle analysis showed that cell division was more active in SfB than in ScA. LDH release in the supernatant increased much earlier in SfB than in ScA (one vs. five days), but trypan blue counts showed no apparent difference in the viability of the cultures. Apoptosis, evidenced by annexin V-FITC/PI staining, could be detected in the population of suspension cells detached from microcarriers, but not among adherent cells; positivity of the TUNEL assay occurred later than that of the annexin V-FITC/PI staining. Our data indicate that the lower cell yield in SfB,compared with that in ScA, results from a higher cell death rate. Apparently, cells die from apoptosis followed by secondary necrosis.</p></abstract><kwd-group xml:lang="en"><kwd>apoptosis</kwd><kwd>cell cycle</kwd><kwd>lactate dehydrogenase</kwd><kwd>microcarriers</kwd><kwd>necrosis</kwd><kwd>serum-free medium</kwd><kwd>TUNEL</kwd><kwd>Vero cells</kwd></kwd-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© Kluwer Academic Publishers 2001</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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