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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Kinetics and metabolic specificities of Vero cells in bioreactor cultures with serum-free medium</title><author><name sortKey="Quesney, Sebastien" sort="Quesney, Sebastien" uniqKey="Quesney S" first="Sébastien" last="Quesney">Sébastien Quesney</name><affiliation><nlm:aff id="Aff1">bioMérieux, Chemin de l'orme, Marcy L'etoile, F-69280 France</nlm:aff></affiliation></author><author><name sortKey="Marc, Annie" sort="Marc, Annie" uniqKey="Marc A" first="Annie" last="Marc">Annie Marc</name><affiliation><nlm:aff id="Aff2">Laboratoire des Sciences du Génie Chimique, CNRS, UPR 6811, ENSIC, 1 rue Granville, Nancy, F-54001 France</nlm:aff></affiliation></author><author><name sortKey="Gerdil, Catherine" sort="Gerdil, Catherine" uniqKey="Gerdil C" first="Catherine" last="Gerdil">Catherine Gerdil</name><affiliation><nlm:aff id="Aff3">Aventis Pasteur, Campus Mérieux, 1541 Avenue Marcel Mérieux, Marcy L'etoile, F-69280 France</nlm:aff></affiliation></author><author><name sortKey="Gimenez, Cyrille" sort="Gimenez, Cyrille" uniqKey="Gimenez C" first="Cyrille" last="Gimenez">Cyrille Gimenez</name><affiliation><nlm:aff id="Aff3">Aventis Pasteur, Campus Mérieux, 1541 Avenue Marcel Mérieux, Marcy L'etoile, F-69280 France</nlm:aff></affiliation></author><author><name sortKey="Marvel, Jacqueline" sort="Marvel, Jacqueline" uniqKey="Marvel J" first="Jacqueline" last="Marvel">Jacqueline Marvel</name><affiliation><nlm:aff id="Aff4">Centre Européen de Recherche en Virologie et Immunologie, INSERM, unité 503, 21 Avenue Tony Garnier, Lyon, F-69365 France</nlm:aff></affiliation></author><author><name sortKey="Richard, Yves" sort="Richard, Yves" uniqKey="Richard Y" first="Yves" last="Richard">Yves Richard</name><affiliation><nlm:aff id="Aff5">Ecole Nationale Vétérinaire de Lyon, 1 Avenue Bourgelat B.P. 83, Marcy L'etoile, F-69280 France</nlm:aff></affiliation></author><author><name sortKey="Meignier, Bernard" sort="Meignier, Bernard" uniqKey="Meignier B" first="Bernard" last="Meignier">Bernard Meignier</name><affiliation><nlm:aff id="Aff3">Aventis Pasteur, Campus Mérieux, 1541 Avenue Marcel Mérieux, Marcy L'etoile, F-69280 France</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">19002923</idno><idno type="pmc">3449507</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3449507</idno><idno type="RBID">PMC:3449507</idno><idno type="doi">10.1023/A:1026185615650</idno><date when="2003">2003</date><idno type="wicri:Area/Pmc/Corpus">000017</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000017</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Kinetics and metabolic specificities of Vero cells in bioreactor cultures with serum-free medium</title><author><name sortKey="Quesney, Sebastien" sort="Quesney, Sebastien" uniqKey="Quesney S" first="Sébastien" last="Quesney">Sébastien Quesney</name><affiliation><nlm:aff id="Aff1">bioMérieux, Chemin de l'orme, Marcy L'etoile, F-69280 France</nlm:aff></affiliation></author><author><name sortKey="Marc, Annie" sort="Marc, Annie" uniqKey="Marc A" first="Annie" last="Marc">Annie Marc</name><affiliation><nlm:aff id="Aff2">Laboratoire des Sciences du Génie Chimique, CNRS, UPR 6811, ENSIC, 1 rue Granville, Nancy, F-54001 France</nlm:aff></affiliation></author><author><name sortKey="Gerdil, Catherine" sort="Gerdil, Catherine" uniqKey="Gerdil C" first="Catherine" last="Gerdil">Catherine Gerdil</name><affiliation><nlm:aff id="Aff3">Aventis Pasteur, Campus Mérieux, 1541 Avenue Marcel Mérieux, Marcy L'etoile, F-69280 France</nlm:aff></affiliation></author><author><name sortKey="Gimenez, Cyrille" sort="Gimenez, Cyrille" uniqKey="Gimenez C" first="Cyrille" last="Gimenez">Cyrille Gimenez</name><affiliation><nlm:aff id="Aff3">Aventis Pasteur, Campus Mérieux, 1541 Avenue Marcel Mérieux, Marcy L'etoile, F-69280 France</nlm:aff></affiliation></author><author><name sortKey="Marvel, Jacqueline" sort="Marvel, Jacqueline" uniqKey="Marvel J" first="Jacqueline" last="Marvel">Jacqueline Marvel</name><affiliation><nlm:aff id="Aff4">Centre Européen de Recherche en Virologie et Immunologie, INSERM, unité 503, 21 Avenue Tony Garnier, Lyon, F-69365 France</nlm:aff></affiliation></author><author><name sortKey="Richard, Yves" sort="Richard, Yves" uniqKey="Richard Y" first="Yves" last="Richard">Yves Richard</name><affiliation><nlm:aff id="Aff5">Ecole Nationale Vétérinaire de Lyon, 1 Avenue Bourgelat B.P. 83, Marcy L'etoile, F-69280 France</nlm:aff></affiliation></author><author><name sortKey="Meignier, Bernard" sort="Meignier, Bernard" uniqKey="Meignier B" first="Bernard" last="Meignier">Bernard Meignier</name><affiliation><nlm:aff id="Aff3">Aventis Pasteur, Campus Mérieux, 1541 Avenue Marcel Mérieux, Marcy L'etoile, F-69280 France</nlm:aff></affiliation></author></analytic><series><title level="j">Cytotechnology</title><idno type="ISSN">0920-9069</idno><idno type="eISSN">1573-0778</idno><imprint><date when="2003">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The aim of this study was to understand the metabolism kinetics of Vero cells grown on microcarriers in bioreactors in serum-free medium (SFM). We sought to determine what nutrients are essential for Vero cells and how they are consumed. Contrary to glucose and to most of the amino acids, glutamine and serine were very quickly depleted in this medium and can be supposed to be responsible for cell apoptosis. Lactate and ammonium ions did not reach toxic levels for Vero cells. We payed more attention to the lactate metabolism. Usually we observed that after about 2 days lactate was consumed in serum-containing media, but its concentration plateaud in SFM. Moreover, the addition of serum in SFM provoked lactate consumption and the rate of glucose and glutamine consumption was twice as high as in the SFM not supplemented with serum. The depletion of glutamine and serine and the metabolic deviations leading to a shortage of intermediate products required for other metabolic pathways probably contribute to the lower cell yield and higher cell death rate in SFM.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Cytotechnology</journal-id><journal-id journal-id-type="iso-abbrev">Cytotechnology</journal-id><journal-title-group><journal-title>Cytotechnology</journal-title></journal-title-group><issn pub-type="ppub">0920-9069</issn><issn pub-type="epub">1573-0778</issn><publisher><publisher-name>Kluwer Academic Publishers</publisher-name><publisher-loc>Dordrecht</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">19002923</article-id><article-id pub-id-type="pmc">3449507</article-id><article-id pub-id-type="publisher-id">5119467</article-id><article-id pub-id-type="doi">10.1023/A:1026185615650</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Kinetics and metabolic specificities of Vero cells in bioreactor cultures with serum-free medium</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Quesney</surname><given-names>Sébastien</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Marc</surname><given-names>Annie</given-names></name><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Gerdil</surname><given-names>Catherine</given-names></name><xref ref-type="aff" rid="Aff3">3</xref></contrib><contrib contrib-type="author"><name><surname>Gimenez</surname><given-names>Cyrille</given-names></name><address><email>Cyrille.Gimenez@aventis.com</email></address><xref ref-type="aff" rid="Aff3">3</xref></contrib><contrib contrib-type="author"><name><surname>Marvel</surname><given-names>Jacqueline</given-names></name><xref ref-type="aff" rid="Aff4">4</xref></contrib><contrib contrib-type="author"><name><surname>Richard</surname><given-names>Yves</given-names></name><xref ref-type="aff" rid="Aff5">5</xref></contrib><contrib contrib-type="author"><name><surname>Meignier</surname><given-names>Bernard</given-names></name><xref ref-type="aff" rid="Aff3">3</xref></contrib><aff id="Aff1"><label>1</label>bioMérieux, Chemin de l'orme, Marcy L'etoile, F-69280 France</aff><aff id="Aff2"><label>2</label>Laboratoire des Sciences du Génie Chimique, CNRS, UPR 6811, ENSIC, 1 rue Granville, Nancy, F-54001 France</aff><aff id="Aff3"><label>3</label>Aventis Pasteur, Campus Mérieux, 1541 Avenue Marcel Mérieux, Marcy L'etoile, F-69280 France</aff><aff id="Aff4"><label>4</label>Centre Européen de Recherche en Virologie et Immunologie, INSERM, unité 503, 21 Avenue Tony Garnier, Lyon, F-69365 France</aff><aff id="Aff5"><label>5</label>Ecole Nationale Vétérinaire de Lyon, 1 Avenue Bourgelat B.P. 83, Marcy L'etoile, F-69280 France</aff></contrib-group><pub-date pub-type="ppub"><month>5</month><year>2003</year></pub-date><volume>42</volume><issue>1</issue><fpage>1</fpage><lpage>11</lpage><permissions><copyright-statement>© Kluwer Academic Publishers 2003</copyright-statement></permissions><abstract id="Abs1"><p>The aim of this study was to understand the metabolism kinetics of Vero cells grown on microcarriers in bioreactors in serum-free medium (SFM). We sought to determine what nutrients are essential for Vero cells and how they are consumed. Contrary to glucose and to most of the amino acids, glutamine and serine were very quickly depleted in this medium and can be supposed to be responsible for cell apoptosis. Lactate and ammonium ions did not reach toxic levels for Vero cells. We payed more attention to the lactate metabolism. Usually we observed that after about 2 days lactate was consumed in serum-containing media, but its concentration plateaud in SFM. Moreover, the addition of serum in SFM provoked lactate consumption and the rate of glucose and glutamine consumption was twice as high as in the SFM not supplemented with serum. The depletion of glutamine and serine and the metabolic deviations leading to a shortage of intermediate products required for other metabolic pathways probably contribute to the lower cell yield and higher cell death rate in SFM.</p></abstract><kwd-group xml:lang="en"><kwd>Amino acid</kwd><kwd>Glucose</kwd><kwd>Lactate</kwd><kwd>Metabolism</kwd><kwd>Microcarriers</kwd><kwd>Serum-free medium</kwd><kwd>Vero cells</kwd></kwd-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© Kluwer Academic Publishers 2003</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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