Limiting Cell Aggregation During Mesenchymal Stem Cell Expansion on Microcarriers
Identifieur interne : 000823 ( Main/Curation ); précédent : 000822; suivant : 000824Limiting Cell Aggregation During Mesenchymal Stem Cell Expansion on Microcarriers
Auteurs : Caroline Ferrari [France] ; Frederique Balandras [France] ; Emmanuel Guedon [France] ; Eric Olmos [France] ; Isabelle Chevalot [France] ; Annie Marc [France]Source :
- Biotechnology Progress [ 8756-7938 ] ; 2012.
English descriptors
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Abstract
Mesenchymal stem cells (MSC) are known to be a valuable cell source for tissue engineering and regenerative medicine. However, one of the main limiting steps in their clinical use is the amplification step. MSC expansion on microcarriers has emerged during the last few years, fulfilling the lack of classical T-flasks expansion. Even if the therapeutic potential of MSC as aggregates has been recently highlighted, cell aggregation during expansion has to be avoided. Thus, MSC culture on microcarriers has still to be improved, notably concerning cell aggregation prevention. The aim of this study was to limit cell aggregation during MSC expansion on Cytodex-1 (R), by evaluating the impact of several culture parameters. First, MSC cultures were performed at different agitation rates (0, 25, and 75 rpm) and different initial cell densities (25 and 50 x 10(6) cell g(-1) Cytodex-1 (R)). Then, the MSC aggregates were put into contact with additional available surfaces (T-flask, fresh and used Cytodex-1 (R)) at different times (before and after cell aggregation). The results showed that cell aggregation was partly induced by agitation and prevented in static cultures. Moreover, cell aggregation was dependent on cell density and correlated with a decrease in the total cell number. It was however shown that the aggregated organization could be dissociated when in contact with additional surfaces such as T-flasks or fresh Cytodex-1 (R) carriers. Finally, cell aggregation could be successfully limited in spinner flask by adding fresh Cytodex-1 (R) carriers before its onset. Those results indicated that MSC expansion on agitated Cytodex-1 (R) microcarriers could be performed without cell aggregation, avoiding a decrease in total cell number.
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DOI: 10.1002/btpr.1527
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Hal:hal-00777492Le document en format XML
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ENSIC, 1 rue de Grandville BP 20451, 54001 Nancy Cedex</addrLine><country key="FR"></country></address><ref type="url">http://lrgp.univ-lorraine.fr/</ref></desc><listRelation><relation active="#struct-413289" type="direct"></relation><relation name="UMR7274" active="#struct-441569" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-413289" type="direct"><org type="institution" xml:id="struct-413289" status="VALID"><idno type="IdRef">157040569</idno><idno type="IdUnivLorraine">[UL]100--</idno><orgName>Université de Lorraine</orgName><orgName type="acronym">UL</orgName><date type="start">2012-01-01</date><desc><address><addrLine>34 cours Léopold - CS 25233 - 54052 Nancy cedex</addrLine><country key="FR"></country></address><ref type="url">http://www.univ-lorraine.fr/</ref></desc></org></tutelle><tutelle name="UMR7274" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"><idno type="ISNI">0000000122597504</idno><idno type="IdRef">02636817X</idno><orgName>Centre National de la Recherche Scientifique</orgName><orgName type="acronym">CNRS</orgName><date type="start">1939-10-19</date><desc><address><country key="FR"></country></address><ref type="url">http://www.cnrs.fr/</ref></desc></org></tutelle></tutelles></hal:affiliation><country>France</country><placeName><settlement type="city">Nancy</settlement><settlement type="city">Metz</settlement><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region></placeName><orgName type="university">Université de Lorraine</orgName></affiliation></author><author><name sortKey="Chevalot, Isabelle" sort="Chevalot, Isabelle" uniqKey="Chevalot I" first="Isabelle" last="Chevalot">Isabelle Chevalot</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-211875" status="VALID"><idno type="IdRef">153068876</idno><idno type="RNSR">201320573K</idno><idno type="IdUnivLorraine">[UL]RQC--</idno><orgName>Laboratoire Réactions et Génie des Procédés</orgName><orgName type="acronym">LRGP</orgName><date type="start">2013-01-01</date><desc><address><addrLine>Université de Lorraine - ENSIC, 1 rue de Grandville BP 20451, 54001 Nancy Cedex</addrLine><country key="FR"></country></address><ref type="url">http://lrgp.univ-lorraine.fr/</ref></desc><listRelation><relation active="#struct-413289" type="direct"></relation><relation name="UMR7274" active="#struct-441569" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-413289" type="direct"><org type="institution" xml:id="struct-413289" status="VALID"><idno type="IdRef">157040569</idno><idno type="IdUnivLorraine">[UL]100--</idno><orgName>Université de Lorraine</orgName><orgName type="acronym">UL</orgName><date type="start">2012-01-01</date><desc><address><addrLine>34 cours Léopold - CS 25233 - 54052 Nancy cedex</addrLine><country key="FR"></country></address><ref type="url">http://www.univ-lorraine.fr/</ref></desc></org></tutelle><tutelle name="UMR7274" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"><idno type="ISNI">0000000122597504</idno><idno type="IdRef">02636817X</idno><orgName>Centre National de la Recherche Scientifique</orgName><orgName type="acronym">CNRS</orgName><date type="start">1939-10-19</date><desc><address><country key="FR"></country></address><ref type="url">http://www.cnrs.fr/</ref></desc></org></tutelle></tutelles></hal:affiliation><country>France</country><placeName><settlement type="city">Nancy</settlement><settlement type="city">Metz</settlement><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region></placeName><orgName type="university">Université de Lorraine</orgName></affiliation></author><author><name sortKey="Marc, Annie" sort="Marc, Annie" uniqKey="Marc A" first="Annie" last="Marc">Annie Marc</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-211875" status="VALID"><idno type="IdRef">153068876</idno><idno type="RNSR">201320573K</idno><idno type="IdUnivLorraine">[UL]RQC--</idno><orgName>Laboratoire Réactions et Génie des Procédés</orgName><orgName type="acronym">LRGP</orgName><date type="start">2013-01-01</date><desc><address><addrLine>Université de Lorraine - ENSIC, 1 rue de Grandville BP 20451, 54001 Nancy Cedex</addrLine><country key="FR"></country></address><ref type="url">http://lrgp.univ-lorraine.fr/</ref></desc><listRelation><relation active="#struct-413289" type="direct"></relation><relation name="UMR7274" active="#struct-441569" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-413289" type="direct"><org type="institution" xml:id="struct-413289" status="VALID"><idno type="IdRef">157040569</idno><idno type="IdUnivLorraine">[UL]100--</idno><orgName>Université de Lorraine</orgName><orgName type="acronym">UL</orgName><date type="start">2012-01-01</date><desc><address><addrLine>34 cours Léopold - CS 25233 - 54052 Nancy cedex</addrLine><country key="FR"></country></address><ref type="url">http://www.univ-lorraine.fr/</ref></desc></org></tutelle><tutelle name="UMR7274" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"><idno type="ISNI">0000000122597504</idno><idno type="IdRef">02636817X</idno><orgName>Centre National de la Recherche Scientifique</orgName><orgName type="acronym">CNRS</orgName><date type="start">1939-10-19</date><desc><address><country key="FR"></country></address><ref type="url">http://www.cnrs.fr/</ref></desc></org></tutelle></tutelles></hal:affiliation><country>France</country><placeName><settlement type="city">Nancy</settlement><settlement type="city">Metz</settlement><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region></placeName><orgName type="university">Université de Lorraine</orgName></affiliation></author></analytic><idno type="DOI">10.1002/btpr.1527</idno><series><title level="j">Biotechnology Progress</title><idno type="ISSN">8756-7938</idno><imprint><date type="datePub">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="mix" xml:lang="en"><term>ADHESION</term><term>CULTIVATION SYSTEM</term><term>DIFFERENTIATION</term><term>INTEGRIN</term><term>SERUM</term><term>SPHEROIDS</term><term>SPIN CULTURE</term><term>STROMAL CELLS</term><term>VERO CELLS</term><term>WETTABILITY</term><term>aggregation</term><term>mesenchymal stem cells</term><term>microcarriers</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Mesenchymal stem cells (MSC) are known to be a valuable cell source for tissue engineering and regenerative medicine. However, one of the main limiting steps in their clinical use is the amplification step. MSC expansion on microcarriers has emerged during the last few years, fulfilling the lack of classical T-flasks expansion. Even if the therapeutic potential of MSC as aggregates has been recently highlighted, cell aggregation during expansion has to be avoided. Thus, MSC culture on microcarriers has still to be improved, notably concerning cell aggregation prevention. The aim of this study was to limit cell aggregation during MSC expansion on Cytodex-1 (R), by evaluating the impact of several culture parameters. First, MSC cultures were performed at different agitation rates (0, 25, and 75 rpm) and different initial cell densities (25 and 50 x 10(6) cell g(-1) Cytodex-1 (R)). Then, the MSC aggregates were put into contact with additional available surfaces (T-flask, fresh and used Cytodex-1 (R)) at different times (before and after cell aggregation). The results showed that cell aggregation was partly induced by agitation and prevented in static cultures. Moreover, cell aggregation was dependent on cell density and correlated with a decrease in the total cell number. It was however shown that the aggregated organization could be dissociated when in contact with additional surfaces such as T-flasks or fresh Cytodex-1 (R) carriers. Finally, cell aggregation could be successfully limited in spinner flask by adding fresh Cytodex-1 (R) carriers before its onset. Those results indicated that MSC expansion on agitated Cytodex-1 (R) microcarriers could be performed without cell aggregation, avoiding a decrease in total cell number.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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