LrgpV1, Hal, Corpus, bibRecord, 000280

Critical agitation for microcarrier suspension in orbital shaken bioreactors : Experimental study and dimensional analysis

Identifieur interne : 000280 ( Hal/Corpus ); précédent : 000279; suivant : 000281

Critical agitation for microcarrier suspension in orbital shaken bioreactors : Experimental study and dimensional analysis

Auteurs : Eric Olmos ; Karine Loubiere ; Céline Martin ; Guillaume Delaplace ; Annie Marc

Source :

Chemical Engineering Science [ 0009-2509 ] ; 2014.

RBID : Hal:hal-01107247

English descriptors

mix :
- Dimensional analysis, Experimental determination, Microcarrier suspension, Shaken flasks.

Abstract

Orbital shaken bioreactors are widely used at the laboratory scale for the culture of animal cells in suspension mode. In the case of adherent-dependent cell lines,the choice of agitation conditions at which all particles are just-suspended (or attain complete suspension) has often to be determined.Indeed, with orbital shaken bioreactors,this choice results from the combination of two parameters : the orbital diameter and the agitation rate. That is why an experimental protocol for the determination of critical agitation conditions for microcarrier complete suspension has been developed in this paper.It consisted in a side-view visualization of blue-stained particles in shaken Erlenmeyer flasks and cylindrical flasks. 220 experiments representative of animal cell culture conditions have been carried out to study the effect of various operating conditions (bioreactor size and geometry, particle type, density and diameter,liquid viscosity, shaking diameter, filling ratio). Furthermore, a dimensional analysis has been performed, leading to a correlation relating a Froude number (in which the critical agitation Nc for complete particle suspension is sembedded) to four other dimensionless numbers. Then,the critical agitation conditions determined in this paper were analyzed and discussed with respect to the data available in the literature on the flow structure occurring inside the flask.Our findings revealed that high orbital shaking diameters and large cylindrical vessels should be promoted to get microcarriers into suspension at a minimized power dissipation per unit of volume.

Url:

https://hal.archives-ouvertes.fr/hal-01107247

DOI: 10.1016/j.ces.2014.08.063

Links to Exploration step

Hal:hal-01107247

Le document en format XML

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<front><div type="abstract" xml:lang="en">Orbital shaken bioreactors are widely used at the laboratory scale for the culture of animal cells in suspension mode. In the case of adherent-dependent cell lines,the choice of agitation conditions at which all particles are just-suspended (or attain complete suspension) has often to be determined.Indeed, with orbital shaken bioreactors,this choice results from the combination of two parameters : the orbital diameter and the agitation rate. That is why an experimental protocol for the determination of critical agitation conditions for microcarrier complete suspension has been developed in this paper.It consisted in a side-view visualization of blue-stained particles in shaken Erlenmeyer flasks and cylindrical flasks. 220 experiments representative of animal cell culture conditions have been carried out to study the effect of various operating conditions (bioreactor size and geometry, particle type, density and diameter,liquid viscosity, shaking diameter, filling ratio). Furthermore, a dimensional analysis has been performed, leading to a correlation relating  a Froude number (in which the critical agitation Nc for complete particle suspension is sembedded) to four other dimensionless numbers. Then,the critical agitation conditions determined in this paper were analyzed and discussed with respect to the data available in the literature on the flow structure occurring inside the flask.Our findings revealed that high orbital shaking diameters and large cylindrical vessels should be promoted to get microcarriers into suspension at a minimized power dissipation per unit of volume.</div>
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<idno type="halRefHtml">Chemical Engineering Science, Elsevier, 2014, vol. 122, pp. 545-554. <10.1016/j.ces.2014.08.063></idno>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Critical agitation for microcarrier suspension in orbital shaken bioreactors : Experimental study and dimensional analysis</title>
<author role="aut"><persName><forename type="first">Eric</forename>
<surname>Olmos</surname>
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<author role="aut"><persName><forename type="first">Céline</forename>
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<author role="aut"><persName><forename type="first">Guillaume</forename>
<surname>Delaplace</surname>
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<idno type="halAuthorId">775093</idno>
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<author role="aut"><persName><forename type="first">Annie</forename>
<surname>MARC</surname>
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<title level="j">Chemical Engineering Science</title>
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<biblScope unit="pp">pp. 545-554</biblScope>
<date type="datePub">2014</date>
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<idno type="doi">10.1016/j.ces.2014.08.063</idno>
<ref type="seeAlso">http://oatao.univ-toulouse.fr/12197</ref>
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<profileDesc><langUsage><language ident="en">English</language>
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<textClass><keywords scheme="author"><term xml:lang="en">Shaken flasks</term>
<term xml:lang="en">Dimensional analysis</term>
<term xml:lang="en">Experimental determination</term>
<term xml:lang="en">Microcarrier suspension</term>
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<abstract xml:lang="en">Orbital shaken bioreactors are widely used at the laboratory scale for the culture of animal cells in suspension mode. In the case of adherent-dependent cell lines,the choice of agitation conditions at which all particles are just-suspended (or attain complete suspension) has often to be determined.Indeed, with orbital shaken bioreactors,this choice results from the combination of two parameters : the orbital diameter and the agitation rate. That is why an experimental protocol for the determination of critical agitation conditions for microcarrier complete suspension has been developed in this paper.It consisted in a side-view visualization of blue-stained particles in shaken Erlenmeyer flasks and cylindrical flasks. 220 experiments representative of animal cell culture conditions have been carried out to study the effect of various operating conditions (bioreactor size and geometry, particle type, density and diameter,liquid viscosity, shaking diameter, filling ratio). Furthermore, a dimensional analysis has been performed, leading to a correlation relating  a Froude number (in which the critical agitation Nc for complete particle suspension is sembedded) to four other dimensionless numbers. Then,the critical agitation conditions determined in this paper were analyzed and discussed with respect to the data available in the literature on the flow structure occurring inside the flask.Our findings revealed that high orbital shaking diameters and large cylindrical vessels should be promoted to get microcarriers into suspension at a minimized power dissipation per unit of volume.</abstract>
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Critical agitation for microcarrier suspension in orbital shaken bioreactors : Experimental study and dimensional analysis

Critical agitation for microcarrier suspension in orbital shaken bioreactors : Experimental study and dimensional analysis

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