Modelling the separation of small peptides by cation exchange chromatography
Identifieur interne : 000A76 ( Hal/Checkpoint ); précédent : 000A75; suivant : 000A77Modelling the separation of small peptides by cation exchange chromatography
Auteurs : Christelle Harscoat-Schiavo [France] ; Fenomanana Raminosoa ; Evelyne Ronat-Heit [France] ; Régis Vanderesse [France] ; Ivan Marc [France]Source :
- Journal of Separation Science [ 1615-9306 ] ; 2010-08.
English descriptors
- mix :
Abstract
Cation-exchange chromatography was applied for the separation of short synthetic peptide standards, with various charges and hydrophobicities. The methodology to develop simple, reproducible and accurate models, based on physicochemical peptide properties, was described. A multilinear regression method was used for the calculation of the models, and descriptors were chosen according to the observed phenomena. The predictive and interpretative ability of the chromatographic models was evaluated considering cross-validated data (root mean-squared error of calibration, root mean-squared error, root mean-squared error of cross-validation and the Fisher ratio). Hydrophobic coefficients for amino acids were calculated with or without consideration of peptide sequences. A simple model, with only two parameters (charge and hydrophobic coefficient) was built. It enabled an accurate prediction of short peptide elution (up to nine residues). As the model was intended for further characterization of complex mixtures of unknown peptides, some mixtures were analyzed to investigate possible interactions between molecules. Peptides eluted in exactly the same pattern as when injected alone, supporting the use of these models for complex mixtures of small peptides.
Url:
DOI: 10.1002/jssc.201000112
Links toward previous steps (curation, corpus...)
Links to Exploration step
Hal:hal-00785045Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Modelling the separation of small peptides by cation exchange chromatography</title>
<author><name sortKey="Harscoat Schiavo, Christelle" sort="Harscoat Schiavo, Christelle" uniqKey="Harscoat Schiavo C" first="Christelle" last="Harscoat-Schiavo">Christelle Harscoat-Schiavo</name>
<affiliation wicri:level="1"><hal:affiliation type="researchteam" xml:id="struct-405377" status="INCOMING"><orgName>BioProMo</orgName>
<desc><address><country key="FR"></country>
</address>
</desc>
<listRelation><relation active="#struct-211875" type="direct"></relation>
<relation active="#struct-413289" type="indirect"></relation>
<relation name="UMR7274" active="#struct-441569" type="indirect"></relation>
</listRelation>
<tutelles><tutelle active="#struct-211875" type="direct"><org type="laboratory" xml:id="struct-211875" status="VALID"><idno type="IdRef">153068876</idno>
<idno type="RNSR">201320573K</idno>
<idno type="IdUnivLorraine">[UL]RQC--</idno>
<orgName>Laboratoire Réactions et Génie des Procédés</orgName>
<orgName type="acronym">LRGP</orgName>
<date type="start">2013-01-01</date>
<desc><address><addrLine>Université de Lorraine - ENSIC, 1 rue de Grandville BP 20451, 54001 Nancy Cedex</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://lrgp.univ-lorraine.fr/</ref>
</desc>
<listRelation><relation active="#struct-413289" type="direct"></relation>
<relation name="UMR7274" active="#struct-441569" type="direct"></relation>
</listRelation>
</org>
</tutelle>
<tutelle active="#struct-413289" type="indirect"><org type="institution" xml:id="struct-413289" status="VALID"><idno type="IdRef">157040569</idno>
<idno type="IdUnivLorraine">[UL]100--</idno>
<orgName>Université de Lorraine</orgName>
<orgName type="acronym">UL</orgName>
<date type="start">2012-01-01</date>
<desc><address><addrLine>34 cours Léopold - CS 25233 - 54052 Nancy cedex</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-lorraine.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7274" active="#struct-441569" type="indirect"><org type="institution" xml:id="struct-441569" status="VALID"><idno type="ISNI">0000000122597504</idno>
<idno type="IdRef">02636817X</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc><address><country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Nancy</settlement>
<settlement type="city">Metz</settlement>
<region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Lorraine (région)</region>
</placeName>
<orgName type="university">Université de Lorraine</orgName>
</affiliation>
</author>
<author><name sortKey="Raminosoa, Fenomanana" sort="Raminosoa, Fenomanana" uniqKey="Raminosoa F" first="Fenomanana" last="Raminosoa">Fenomanana Raminosoa</name>
</author>
<author><name sortKey="Ronat Heit, Evelyne" sort="Ronat Heit, Evelyne" uniqKey="Ronat Heit E" first="Evelyne" last="Ronat-Heit">Evelyne Ronat-Heit</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-211875" status="VALID"><idno type="IdRef">153068876</idno>
<idno type="RNSR">201320573K</idno>
<idno type="IdUnivLorraine">[UL]RQC--</idno>
<orgName>Laboratoire Réactions et Génie des Procédés</orgName>
<orgName type="acronym">LRGP</orgName>
<date type="start">2013-01-01</date>
<desc><address><addrLine>Université de Lorraine - ENSIC, 1 rue de Grandville BP 20451, 54001 Nancy Cedex</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://lrgp.univ-lorraine.fr/</ref>
</desc>
<listRelation><relation active="#struct-413289" type="direct"></relation>
<relation name="UMR7274" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle active="#struct-413289" type="direct"><org type="institution" xml:id="struct-413289" status="VALID"><idno type="IdRef">157040569</idno>
<idno type="IdUnivLorraine">[UL]100--</idno>
<orgName>Université de Lorraine</orgName>
<orgName type="acronym">UL</orgName>
<date type="start">2012-01-01</date>
<desc><address><addrLine>34 cours Léopold - CS 25233 - 54052 Nancy cedex</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-lorraine.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7274" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"><idno type="ISNI">0000000122597504</idno>
<idno type="IdRef">02636817X</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc><address><country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Nancy</settlement>
<settlement type="city">Metz</settlement>
<region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Lorraine (région)</region>
</placeName>
<orgName type="university">Université de Lorraine</orgName>
</affiliation>
</author>
<author><name sortKey="Vanderesse, Regis" sort="Vanderesse, Regis" uniqKey="Vanderesse R" first="Régis" last="Vanderesse">Régis Vanderesse</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-439403" status="VALID"><idno type="IdUnivLorraine">[UL]RQE--</idno>
<idno type="RNSR">199112614E</idno>
<idno type="IdRef">152527311</idno>
<orgName>Laboratoire de Chimie Physique Macromoléculaire</orgName>
<orgName type="acronym">LCPM</orgName>
<date type="start">2012-01-01</date>
<desc><address><addrLine>Université de Lorraine - ENSIC, 1 rue Grandville, BP 20451, 54000 Nancy</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://lcpm.univ-lorraine.fr/</ref>
</desc>
<listRelation><relation active="#struct-413289" type="direct"></relation>
<relation name="UMR7375" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle active="#struct-413289" type="direct"><org type="institution" xml:id="struct-413289" status="VALID"><idno type="IdRef">157040569</idno>
<idno type="IdUnivLorraine">[UL]100--</idno>
<orgName>Université de Lorraine</orgName>
<orgName type="acronym">UL</orgName>
<date type="start">2012-01-01</date>
<desc><address><addrLine>34 cours Léopold - CS 25233 - 54052 Nancy cedex</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-lorraine.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7375" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"><idno type="ISNI">0000000122597504</idno>
<idno type="IdRef">02636817X</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc><address><country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Nancy</settlement>
<settlement type="city">Metz</settlement>
<region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Lorraine (région)</region>
</placeName>
<orgName type="university">Université de Lorraine</orgName>
</affiliation>
</author>
<author><name sortKey="Marc, Ivan" sort="Marc, Ivan" uniqKey="Marc I" first="Ivan" last="Marc">Ivan Marc</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-211875" status="VALID"><idno type="IdRef">153068876</idno>
<idno type="RNSR">201320573K</idno>
<idno type="IdUnivLorraine">[UL]RQC--</idno>
<orgName>Laboratoire Réactions et Génie des Procédés</orgName>
<orgName type="acronym">LRGP</orgName>
<date type="start">2013-01-01</date>
<desc><address><addrLine>Université de Lorraine - ENSIC, 1 rue de Grandville BP 20451, 54001 Nancy Cedex</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://lrgp.univ-lorraine.fr/</ref>
</desc>
<listRelation><relation active="#struct-413289" type="direct"></relation>
<relation name="UMR7274" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle active="#struct-413289" type="direct"><org type="institution" xml:id="struct-413289" status="VALID"><idno type="IdRef">157040569</idno>
<idno type="IdUnivLorraine">[UL]100--</idno>
<orgName>Université de Lorraine</orgName>
<orgName type="acronym">UL</orgName>
<date type="start">2012-01-01</date>
<desc><address><addrLine>34 cours Léopold - CS 25233 - 54052 Nancy cedex</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-lorraine.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7274" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"><idno type="ISNI">0000000122597504</idno>
<idno type="IdRef">02636817X</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc><address><country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Nancy</settlement>
<settlement type="city">Metz</settlement>
<region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Lorraine (région)</region>
</placeName>
<orgName type="university">Université de Lorraine</orgName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">HAL</idno>
<idno type="RBID">Hal:hal-00785045</idno>
<idno type="halId">hal-00785045</idno>
<idno type="halUri">https://hal.archives-ouvertes.fr/hal-00785045</idno>
<idno type="url">https://hal.archives-ouvertes.fr/hal-00785045</idno>
<idno type="doi">10.1002/jssc.201000112</idno>
<date when="2010-08">2010-08</date>
<idno type="wicri:Area/Hal/Corpus">000777</idno>
<idno type="wicri:Area/Hal/Curation">000777</idno>
<idno type="wicri:Area/Hal/Checkpoint">000A76</idno>
<idno type="wicri:explorRef" wicri:stream="Hal" wicri:step="Checkpoint">000A76</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Modelling the separation of small peptides by cation exchange chromatography</title>
<author><name sortKey="Harscoat Schiavo, Christelle" sort="Harscoat Schiavo, Christelle" uniqKey="Harscoat Schiavo C" first="Christelle" last="Harscoat-Schiavo">Christelle Harscoat-Schiavo</name>
<affiliation wicri:level="1"><hal:affiliation type="researchteam" xml:id="struct-405377" status="INCOMING"><orgName>BioProMo</orgName>
<desc><address><country key="FR"></country>
</address>
</desc>
<listRelation><relation active="#struct-211875" type="direct"></relation>
<relation active="#struct-413289" type="indirect"></relation>
<relation name="UMR7274" active="#struct-441569" type="indirect"></relation>
</listRelation>
<tutelles><tutelle active="#struct-211875" type="direct"><org type="laboratory" xml:id="struct-211875" status="VALID"><idno type="IdRef">153068876</idno>
<idno type="RNSR">201320573K</idno>
<idno type="IdUnivLorraine">[UL]RQC--</idno>
<orgName>Laboratoire Réactions et Génie des Procédés</orgName>
<orgName type="acronym">LRGP</orgName>
<date type="start">2013-01-01</date>
<desc><address><addrLine>Université de Lorraine - ENSIC, 1 rue de Grandville BP 20451, 54001 Nancy Cedex</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://lrgp.univ-lorraine.fr/</ref>
</desc>
<listRelation><relation active="#struct-413289" type="direct"></relation>
<relation name="UMR7274" active="#struct-441569" type="direct"></relation>
</listRelation>
</org>
</tutelle>
<tutelle active="#struct-413289" type="indirect"><org type="institution" xml:id="struct-413289" status="VALID"><idno type="IdRef">157040569</idno>
<idno type="IdUnivLorraine">[UL]100--</idno>
<orgName>Université de Lorraine</orgName>
<orgName type="acronym">UL</orgName>
<date type="start">2012-01-01</date>
<desc><address><addrLine>34 cours Léopold - CS 25233 - 54052 Nancy cedex</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-lorraine.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7274" active="#struct-441569" type="indirect"><org type="institution" xml:id="struct-441569" status="VALID"><idno type="ISNI">0000000122597504</idno>
<idno type="IdRef">02636817X</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc><address><country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Nancy</settlement>
<settlement type="city">Metz</settlement>
<region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Lorraine (région)</region>
</placeName>
<orgName type="university">Université de Lorraine</orgName>
</affiliation>
</author>
<author><name sortKey="Raminosoa, Fenomanana" sort="Raminosoa, Fenomanana" uniqKey="Raminosoa F" first="Fenomanana" last="Raminosoa">Fenomanana Raminosoa</name>
</author>
<author><name sortKey="Ronat Heit, Evelyne" sort="Ronat Heit, Evelyne" uniqKey="Ronat Heit E" first="Evelyne" last="Ronat-Heit">Evelyne Ronat-Heit</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-211875" status="VALID"><idno type="IdRef">153068876</idno>
<idno type="RNSR">201320573K</idno>
<idno type="IdUnivLorraine">[UL]RQC--</idno>
<orgName>Laboratoire Réactions et Génie des Procédés</orgName>
<orgName type="acronym">LRGP</orgName>
<date type="start">2013-01-01</date>
<desc><address><addrLine>Université de Lorraine - ENSIC, 1 rue de Grandville BP 20451, 54001 Nancy Cedex</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://lrgp.univ-lorraine.fr/</ref>
</desc>
<listRelation><relation active="#struct-413289" type="direct"></relation>
<relation name="UMR7274" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle active="#struct-413289" type="direct"><org type="institution" xml:id="struct-413289" status="VALID"><idno type="IdRef">157040569</idno>
<idno type="IdUnivLorraine">[UL]100--</idno>
<orgName>Université de Lorraine</orgName>
<orgName type="acronym">UL</orgName>
<date type="start">2012-01-01</date>
<desc><address><addrLine>34 cours Léopold - CS 25233 - 54052 Nancy cedex</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-lorraine.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7274" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"><idno type="ISNI">0000000122597504</idno>
<idno type="IdRef">02636817X</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc><address><country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Nancy</settlement>
<settlement type="city">Metz</settlement>
<region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Lorraine (région)</region>
</placeName>
<orgName type="university">Université de Lorraine</orgName>
</affiliation>
</author>
<author><name sortKey="Vanderesse, Regis" sort="Vanderesse, Regis" uniqKey="Vanderesse R" first="Régis" last="Vanderesse">Régis Vanderesse</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-439403" status="VALID"><idno type="IdUnivLorraine">[UL]RQE--</idno>
<idno type="RNSR">199112614E</idno>
<idno type="IdRef">152527311</idno>
<orgName>Laboratoire de Chimie Physique Macromoléculaire</orgName>
<orgName type="acronym">LCPM</orgName>
<date type="start">2012-01-01</date>
<desc><address><addrLine>Université de Lorraine - ENSIC, 1 rue Grandville, BP 20451, 54000 Nancy</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://lcpm.univ-lorraine.fr/</ref>
</desc>
<listRelation><relation active="#struct-413289" type="direct"></relation>
<relation name="UMR7375" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle active="#struct-413289" type="direct"><org type="institution" xml:id="struct-413289" status="VALID"><idno type="IdRef">157040569</idno>
<idno type="IdUnivLorraine">[UL]100--</idno>
<orgName>Université de Lorraine</orgName>
<orgName type="acronym">UL</orgName>
<date type="start">2012-01-01</date>
<desc><address><addrLine>34 cours Léopold - CS 25233 - 54052 Nancy cedex</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-lorraine.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7375" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"><idno type="ISNI">0000000122597504</idno>
<idno type="IdRef">02636817X</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc><address><country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Nancy</settlement>
<settlement type="city">Metz</settlement>
<region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Lorraine (région)</region>
</placeName>
<orgName type="university">Université de Lorraine</orgName>
</affiliation>
</author>
<author><name sortKey="Marc, Ivan" sort="Marc, Ivan" uniqKey="Marc I" first="Ivan" last="Marc">Ivan Marc</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-211875" status="VALID"><idno type="IdRef">153068876</idno>
<idno type="RNSR">201320573K</idno>
<idno type="IdUnivLorraine">[UL]RQC--</idno>
<orgName>Laboratoire Réactions et Génie des Procédés</orgName>
<orgName type="acronym">LRGP</orgName>
<date type="start">2013-01-01</date>
<desc><address><addrLine>Université de Lorraine - ENSIC, 1 rue de Grandville BP 20451, 54001 Nancy Cedex</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://lrgp.univ-lorraine.fr/</ref>
</desc>
<listRelation><relation active="#struct-413289" type="direct"></relation>
<relation name="UMR7274" active="#struct-441569" type="direct"></relation>
</listRelation>
<tutelles><tutelle active="#struct-413289" type="direct"><org type="institution" xml:id="struct-413289" status="VALID"><idno type="IdRef">157040569</idno>
<idno type="IdUnivLorraine">[UL]100--</idno>
<orgName>Université de Lorraine</orgName>
<orgName type="acronym">UL</orgName>
<date type="start">2012-01-01</date>
<desc><address><addrLine>34 cours Léopold - CS 25233 - 54052 Nancy cedex</addrLine>
<country key="FR"></country>
</address>
<ref type="url">http://www.univ-lorraine.fr/</ref>
</desc>
</org>
</tutelle>
<tutelle name="UMR7274" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"><idno type="ISNI">0000000122597504</idno>
<idno type="IdRef">02636817X</idno>
<orgName>Centre National de la Recherche Scientifique</orgName>
<orgName type="acronym">CNRS</orgName>
<date type="start">1939-10-19</date>
<desc><address><country key="FR"></country>
</address>
<ref type="url">http://www.cnrs.fr/</ref>
</desc>
</org>
</tutelle>
</tutelles>
</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Nancy</settlement>
<settlement type="city">Metz</settlement>
<region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Lorraine (région)</region>
</placeName>
<orgName type="university">Université de Lorraine</orgName>
</affiliation>
</author>
</analytic>
<idno type="DOI">10.1002/jssc.201000112</idno>
<series><title level="j">Journal of Separation Science</title>
<idno type="ISSN">1615-9306</idno>
<imprint><date type="datePub">2010-08</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="mix" xml:lang="en"><term>AMINO-ACID-COMPOSITION</term>
<term>Cation-exchange chromatography</term>
<term>Hydrophobic index</term>
<term>ION-EXCHANGE</term>
<term>MASS-SPECTROMETRY</term>
<term>Modeling</term>
<term>PERFORMANCE LIQUID-CHROMATOGRAPHY</term>
<term>PREDICTION</term>
<term>PROTEIN</term>
<term>Peptide</term>
<term>RAPESEED</term>
<term>RETENTION TIMES</term>
<term>RP-HPLC</term>
<term>STRONG ANION-EXCHANGE</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Cation-exchange chromatography was applied for the separation of short synthetic peptide standards, with various charges and hydrophobicities. The methodology to develop simple, reproducible and accurate models, based on physicochemical peptide properties, was described. A multilinear regression method was used for the calculation of the models, and descriptors were chosen according to the observed phenomena. The predictive and interpretative ability of the chromatographic models was evaluated considering cross-validated data (root mean-squared error of calibration, root mean-squared error, root mean-squared error of cross-validation and the Fisher ratio). Hydrophobic coefficients for amino acids were calculated with or without consideration of peptide sequences. A simple model, with only two parameters (charge and hydrophobic coefficient) was built. It enabled an accurate prediction of short peptide elution (up to nine residues). As the model was intended for further characterization of complex mixtures of unknown peptides, some mixtures were analyzed to investigate possible interactions between molecules. Peptides eluted in exactly the same pattern as when injected alone, supporting the use of these models for complex mixtures of small peptides.</div>
</front>
</TEI>
<hal api="V3"><titleStmt><title xml:lang="en">Modelling the separation of small peptides by cation exchange chromatography</title>
<author role="crp"><persName><forename type="first">Christelle</forename>
<surname>Harscoat-Schiavo</surname>
</persName>
<email>Christelle.Harscoat@ensic.inpl-nancy.fr</email>
<idno type="halauthor">807021</idno>
<affiliation ref="#struct-405377"></affiliation>
</author>
<author role="aut"><persName><forename type="first">Fenomanana</forename>
<surname>Raminosoa</surname>
</persName>
<email></email>
<idno type="halauthor">813624</idno>
</author>
<author role="aut"><persName><forename type="first">Evelyne</forename>
<surname>Ronat-Heit</surname>
</persName>
<email></email>
<idno type="halauthor">807023</idno>
<affiliation ref="#struct-211875"></affiliation>
</author>
<author role="aut"><persName><forename type="first">Régis</forename>
<surname>Vanderesse</surname>
</persName>
<email>Regis.Vanderesse@univ-lorraine.fr</email>
<idno type="halauthor">807024</idno>
<affiliation ref="#struct-439403"></affiliation>
</author>
<author role="aut"><persName><forename type="first">Ivan</forename>
<surname>Marc</surname>
</persName>
<email></email>
<idno type="halauthor">806829</idno>
<affiliation ref="#struct-211875"></affiliation>
</author>
<editor role="depositor"><persName><forename>Gabriel</forename>
<surname>Wild</surname>
</persName>
<email>gabriel.wild@univ-lorraine.fr</email>
</editor>
</titleStmt>
<editionStmt><edition n="v1" type="current"><date type="whenSubmitted">2013-02-05 11:18:14</date>
<date type="whenModified">2016-05-10 16:53:50</date>
<date type="whenReleased">2013-02-05 11:18:14</date>
<date type="whenProduced">2010-08</date>
</edition>
<respStmt><resp>contributor</resp>
<name key="179637"><persName><forename>Gabriel</forename>
<surname>Wild</surname>
</persName>
<email>gabriel.wild@univ-lorraine.fr</email>
</name>
</respStmt>
</editionStmt>
<publicationStmt><distributor>CCSD</distributor>
<idno type="halId">hal-00785045</idno>
<idno type="halUri">https://hal.archives-ouvertes.fr/hal-00785045</idno>
<idno type="halBibtex">harscoatschiavo:hal-00785045</idno>
<idno type="halRefHtml">Journal of Separation Science, Wiley-VCH Verlag, 2010, 33 (16), pp.2447-2457. <10.1002/jssc.201000112></idno>
<idno type="halRef">Journal of Separation Science, Wiley-VCH Verlag, 2010, 33 (16), pp.2447-2457. <10.1002/jssc.201000112></idno>
</publicationStmt>
<seriesStmt><idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
<idno type="stamp" n="INPL">Institut National Polytechnique de Lorraine</idno>
<idno type="stamp" n="UNIV-LORRAINE">Université de Lorraine</idno>
<idno type="stamp" n="LRGP-UL">LRGP</idno>
</seriesStmt>
<notesStmt><note type="audience" n="2">International</note>
<note type="popular" n="0">No</note>
<note type="peer" n="1">Yes</note>
</notesStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Modelling the separation of small peptides by cation exchange chromatography</title>
<author role="crp"><persName><forename type="first">Christelle</forename>
<surname>Harscoat-Schiavo</surname>
</persName>
<email>Christelle.Harscoat@ensic.inpl-nancy.fr</email>
<idno type="halAuthorId">807021</idno>
<affiliation ref="#struct-405377"></affiliation>
</author>
<author role="aut"><persName><forename type="first">Fenomanana</forename>
<surname>Raminosoa</surname>
</persName>
<idno type="halAuthorId">813624</idno>
</author>
<author role="aut"><persName><forename type="first">Evelyne</forename>
<surname>Ronat-Heit</surname>
</persName>
<idno type="halAuthorId">807023</idno>
<affiliation ref="#struct-211875"></affiliation>
</author>
<author role="aut"><persName><forename type="first">Régis</forename>
<surname>Vanderesse</surname>
</persName>
<email>Regis.Vanderesse@univ-lorraine.fr</email>
<idno type="halAuthorId">807024</idno>
<affiliation ref="#struct-439403"></affiliation>
</author>
<author role="aut"><persName><forename type="first">Ivan</forename>
<surname>Marc</surname>
</persName>
<idno type="halAuthorId">806829</idno>
<affiliation ref="#struct-211875"></affiliation>
</author>
</analytic>
<monogr><idno type="localRef">BIOPROMO</idno>
<idno type="halJournalId" status="VALID">16196</idno>
<idno type="issn">1615-9306</idno>
<idno type="eissn">1615-9314</idno>
<title level="j">Journal of Separation Science</title>
<imprint><publisher>Wiley-VCH Verlag</publisher>
<biblScope unit="volume">33</biblScope>
<biblScope unit="issue">16</biblScope>
<biblScope unit="pp">2447-2457</biblScope>
<date type="datePub">2010-08</date>
</imprint>
</monogr>
<idno type="doi">10.1002/jssc.201000112</idno>
</biblStruct>
</sourceDesc>
<profileDesc><langUsage><language ident="en">English</language>
</langUsage>
<textClass><keywords scheme="author"><term xml:lang="en">Cation-exchange chromatography</term>
<term xml:lang="en">Hydrophobic index</term>
<term xml:lang="en">Modeling</term>
<term xml:lang="en">Peptide</term>
<term xml:lang="en">PERFORMANCE LIQUID-CHROMATOGRAPHY</term>
<term xml:lang="en">AMINO-ACID-COMPOSITION</term>
<term xml:lang="en">STRONG ANION-EXCHANGE</term>
<term xml:lang="en">ION-EXCHANGE</term>
<term xml:lang="en">MASS-SPECTROMETRY</term>
<term xml:lang="en">RETENTION TIMES</term>
<term xml:lang="en">RP-HPLC</term>
<term xml:lang="en">PREDICTION</term>
<term xml:lang="en">PROTEIN</term>
<term xml:lang="en">RAPESEED</term>
</keywords>
<classCode scheme="halDomain" n="spi.gproc">Engineering Sciences [physics]/Chemical and Process Engineering</classCode>
<classCode scheme="halDomain" n="chim.anal">Chemical Sciences/Analytical chemistry</classCode>
<classCode scheme="halTypology" n="ART">Journal articles</classCode>
</textClass>
<abstract xml:lang="en">Cation-exchange chromatography was applied for the separation of short synthetic peptide standards, with various charges and hydrophobicities. The methodology to develop simple, reproducible and accurate models, based on physicochemical peptide properties, was described. A multilinear regression method was used for the calculation of the models, and descriptors were chosen according to the observed phenomena. The predictive and interpretative ability of the chromatographic models was evaluated considering cross-validated data (root mean-squared error of calibration, root mean-squared error, root mean-squared error of cross-validation and the Fisher ratio). Hydrophobic coefficients for amino acids were calculated with or without consideration of peptide sequences. A simple model, with only two parameters (charge and hydrophobic coefficient) was built. It enabled an accurate prediction of short peptide elution (up to nine residues). As the model was intended for further characterization of complex mixtures of unknown peptides, some mixtures were analyzed to investigate possible interactions between molecules. Peptides eluted in exactly the same pattern as when injected alone, supporting the use of these models for complex mixtures of small peptides.</abstract>
</profileDesc>
</hal>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Lorraine/explor/LrgpV1/Data/Hal/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000A76 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Hal/Checkpoint/biblio.hfd -nk 000A76 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Lorraine |area= LrgpV1 |flux= Hal |étape= Checkpoint |type= RBID |clé= Hal:hal-00785045 |texte= Modelling the separation of small peptides by cation exchange chromatography }}
![]() | This area was generated with Dilib version V0.6.32. | ![]() |