Assessment of free dye in solutions of dual-labeled antibody conjugates for in vivo molecular imaging.
Identifieur interne : 001589 ( Main/Exploration ); précédent : 001588; suivant : 001590Assessment of free dye in solutions of dual-labeled antibody conjugates for in vivo molecular imaging.
Auteurs : RBID : pubmed:20458634English descriptors
- KwdEn :
- Antibodies, Monoclonal (chemistry), Antibodies, Monoclonal, Humanized, Calibration, Chromatography, High Pressure Liquid, Coloring Agents (analysis), Electrophoresis, Polyacrylamide Gel, Indicators and Reagents, Indium Radioisotopes (chemistry), Limit of Detection, Pentetic Acid (chemistry), Solutions, Spectroscopy, Near-Infrared.
- MESH :
- chemical , analysis : Coloring Agents.
- chemical , chemistry : Antibodies, Monoclonal, Indium Radioisotopes, Pentetic Acid.
- chemical : Antibodies, Monoclonal, Humanized, Indicators and Reagents, Solutions.
- Calibration, Chromatography, High Pressure Liquid, Electrophoresis, Polyacrylamide Gel, Limit of Detection, Spectroscopy, Near-Infrared.
Abstract
Recent preclinical and clinical studies show that dyes that excite and fluoresce in the near-infrared range may be used for tracking and detecting disease targets in vivo. A method for quantifying free dye molecules in antibody conjugate preparations is required for agent batch release and for translation into the clinic.
DOI: 10.1007/s11307-010-0328-7
PubMed: 20458634
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Assessment of free dye in solutions of dual-labeled antibody conjugates for in vivo molecular imaging.</title><author><name sortKey="Aldrich, Melissa B" uniqKey="Aldrich M">Melissa B Aldrich</name><affiliation wicri:level="1"><nlm:affiliation>Division of Molecular Imaging, The Brown Foundation Institute of Molecular Medicine, University of Texas Health Sciences Center-Houston, Houston, TX, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Molecular Imaging, The Brown Foundation Institute of Molecular Medicine, University of Texas Health Sciences Center-Houston, Houston, TX</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Xuejuan" uniqKey="Wang X">Xuejuan Wang</name></author><author><name sortKey="Hart, Amy" uniqKey="Hart A">Amy Hart</name></author><author><name sortKey="Kwon, Sunkuk" uniqKey="Kwon S">Sunkuk Kwon</name></author><author><name sortKey="Sampath, Lakshmi" uniqKey="Sampath L">Lakshmi Sampath</name></author><author><name sortKey="Marshall, Milton V" uniqKey="Marshall M">Milton V Marshall</name></author><author><name sortKey="Sevick Muraca, Eva M" uniqKey="Sevick Muraca E">Eva M Sevick-Muraca</name></author></titleStmt><publicationStmt><date when="2011">2011</date><idno type="doi">10.1007/s11307-010-0328-7</idno><idno type="RBID">pubmed:20458634</idno><idno type="pmid">20458634</idno><idno type="wicri:Area/Main/Corpus">001937</idno><idno type="wicri:Area/Main/Curation">001937</idno><idno type="wicri:Area/Main/Exploration">001589</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Monoclonal (chemistry)</term><term>Antibodies, Monoclonal, Humanized</term><term>Calibration</term><term>Chromatography, High Pressure Liquid</term><term>Coloring Agents (analysis)</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Indicators and Reagents</term><term>Indium Radioisotopes (chemistry)</term><term>Limit of Detection</term><term>Pentetic Acid (chemistry)</term><term>Solutions</term><term>Spectroscopy, Near-Infrared</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Coloring Agents</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Indium Radioisotopes</term><term>Pentetic Acid</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antibodies, Monoclonal, Humanized</term><term>Indicators and Reagents</term><term>Solutions</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Calibration</term><term>Chromatography, High Pressure Liquid</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Limit of Detection</term><term>Spectroscopy, Near-Infrared</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Recent preclinical and clinical studies show that dyes that excite and fluoresce in the near-infrared range may be used for tracking and detecting disease targets in vivo. A method for quantifying free dye molecules in antibody conjugate preparations is required for agent batch release and for translation into the clinic.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">20458634</PMID><DateCreated><Year>2011</Year><Month>01</Month><Day>19</Day></DateCreated><DateCompleted><Year>2011</Year><Month>06</Month><Day>16</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1860-2002</ISSN><JournalIssue CitedMedium="Internet"><Volume>13</Volume><Issue>1</Issue><PubDate><Year>2011</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging</Title><ISOAbbreviation>Mol Imaging Biol</ISOAbbreviation></Journal><ArticleTitle>Assessment of free dye in solutions of dual-labeled antibody conjugates for in vivo molecular imaging.</ArticleTitle><Pagination><MedlinePgn>32-42</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s11307-010-0328-7</ELocationID><Abstract><AbstractText Label="PURPOSE" NlmCategory="OBJECTIVE">Recent preclinical and clinical studies show that dyes that excite and fluoresce in the near-infrared range may be used for tracking and detecting disease targets in vivo. A method for quantifying free dye molecules in antibody conjugate preparations is required for agent batch release and for translation into the clinic.</AbstractText><AbstractText Label="PROCEDURES" NlmCategory="METHODS">Herein, we developed and validated a SDS-PAGE method to determine the percentage of free IRDye 800 CW in (DTPA)(n)-trastuzumab-(IRDye 800)(m) conjugate sample preparations in which high-performance liquid chromatography (HPLC) assessment of free dye was not possible.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">The SDS-PAGE assay was accurate and valid for free IRDye 800 CW amounts between 38 and 4 mol% of total dye. Gel sample preparation reagent affected the specificity of the assay, and lower and upper limits of quantitation and detection were determined.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">This method may be applicable to other near-infrared dye-conjugated antibody-based imaging agents in which HPLC assessment of purity is not feasible. This validated method for quality assurance will facilitate the translation of dual-labeled antibody conjugates for nuclear and optical imaging.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Aldrich</LastName><ForeName>Melissa B</ForeName><Initials>MB</Initials><Affiliation>Division of Molecular Imaging, The Brown Foundation Institute of Molecular Medicine, University of Texas Health Sciences Center-Houston, Houston, TX, USA.</Affiliation></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Xuejuan</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Hart</LastName><ForeName>Amy</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Kwon</LastName><ForeName>Sunkuk</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Sampath</LastName><ForeName>Lakshmi</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Marshall</LastName><ForeName>Milton V</ForeName><Initials>MV</Initials></Author><Author ValidYN="Y"><LastName>Sevick-Muraca</LastName><ForeName>Eva M</ForeName><Initials>EM</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>U54 CA136404-01</GrantID><Acronym>CA</Acronym><Agency>NCI NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, N.I.H., Extramural</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mol Imaging Biol</MedlineTA><NlmUniqueID>101125610</NlmUniqueID><ISSNLinking>1536-1632</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Antibodies, Monoclonal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Antibodies, Monoclonal, Humanized</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Coloring Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Indicators and Reagents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Indium Radioisotopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Solutions</NameOfSubstance></Chemical><Chemical><RegistryNumber>7A314HQM0I</RegistryNumber><NameOfSubstance>Pentetic Acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>P188ANX8CK</RegistryNumber><NameOfSubstance>trastuzumab</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Antibodies, Monoclonal</DescriptorName><QualifierName MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Antibodies, Monoclonal, Humanized</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Calibration</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Chromatography, High Pressure Liquid</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Coloring Agents</DescriptorName><QualifierName MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Electrophoresis, Polyacrylamide Gel</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Indicators and Reagents</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Indium Radioisotopes</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Limit of Detection</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Pentetic Acid</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Solutions</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Spectroscopy, Near-Infrared</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>5</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>5</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>6</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="doi">10.1007/s11307-010-0328-7</ArticleId><ArticleId IdType="pubmed">20458634</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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