Genomic and Evolutionary Analysis of Two Salmonella enterica Serovar Kentucky Sequence Types Isolated from Bovine and Poultry Sources in North America
Identifieur interne : 000445 ( Pmc/Corpus ); précédent : 000444; suivant : 000446Genomic and Evolutionary Analysis of Two Salmonella enterica Serovar Kentucky Sequence Types Isolated from Bovine and Poultry Sources in North America
Auteurs : Bradd J. Haley ; Seon Woo Kim ; James Pettengill ; Yan Luo ; Jeffrey S. Karns ; Jo Ann S. Van KesselSource :
- PLoS ONE [ 1932-6203 ] ; 2016.
Abstract
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DOI: 10.1371/journal.pone.0161225
PubMed: 27695032
PubMed Central: 5047448
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Genomic and Evolutionary Analysis of Two <italic>Salmonella enterica</italic> Serovar Kentucky Sequence Types Isolated from Bovine and Poultry Sources in North America</title><author><name sortKey="Haley, Bradd J" sort="Haley, Bradd J" uniqKey="Haley B" first="Bradd J." last="Haley">Bradd J. Haley</name><affiliation><nlm:aff id="aff001"><addr-line>Environmental Microbial and Food Safety Laboratory, Beltsville Area Research Center, Agricultural Research Services, United States Department of Agriculture, Beltsville, MD, United States of America</addr-line></nlm:aff></affiliation></author><author><name sortKey="Kim, Seon Woo" sort="Kim, Seon Woo" uniqKey="Kim S" first="Seon Woo" last="Kim">Seon Woo Kim</name><affiliation><nlm:aff id="aff001"><addr-line>Environmental Microbial and Food Safety Laboratory, Beltsville Area Research Center, Agricultural Research Services, United States Department of Agriculture, Beltsville, MD, United States of America</addr-line></nlm:aff></affiliation></author><author><name sortKey="Pettengill, James" sort="Pettengill, James" uniqKey="Pettengill J" first="James" last="Pettengill">James Pettengill</name><affiliation><nlm:aff id="aff002"><addr-line>Office of Analytics and Outreach, Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, College Park, MD, United States of America</addr-line></nlm:aff></affiliation></author><author><name sortKey="Luo, Yan" sort="Luo, Yan" uniqKey="Luo Y" first="Yan" last="Luo">Yan Luo</name><affiliation><nlm:aff id="aff002"><addr-line>Office of Analytics and Outreach, Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, College Park, MD, United States of America</addr-line></nlm:aff></affiliation></author><author><name sortKey="Karns, Jeffrey S" sort="Karns, Jeffrey S" uniqKey="Karns J" first="Jeffrey S." last="Karns">Jeffrey S. Karns</name><affiliation><nlm:aff id="aff001"><addr-line>Environmental Microbial and Food Safety Laboratory, Beltsville Area Research Center, Agricultural Research Services, United States Department of Agriculture, Beltsville, MD, United States of America</addr-line></nlm:aff></affiliation></author><author><name sortKey="Van Kessel, Jo Ann S" sort="Van Kessel, Jo Ann S" uniqKey="Van Kessel J" first="Jo Ann S." last="Van Kessel">Jo Ann S. Van Kessel</name><affiliation><nlm:aff id="aff001"><addr-line>Environmental Microbial and Food Safety Laboratory, Beltsville Area Research Center, Agricultural Research Services, United States Department of Agriculture, Beltsville, MD, United States of America</addr-line></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27695032</idno><idno type="pmc">5047448</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5047448</idno><idno type="RBID">PMC:5047448</idno><idno type="doi">10.1371/journal.pone.0161225</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000445</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000445</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Genomic and Evolutionary Analysis of Two <italic>Salmonella enterica</italic> Serovar Kentucky Sequence Types Isolated from Bovine and Poultry Sources in North America</title><author><name sortKey="Haley, Bradd J" sort="Haley, Bradd J" uniqKey="Haley B" first="Bradd J." last="Haley">Bradd J. Haley</name><affiliation><nlm:aff id="aff001"><addr-line>Environmental Microbial and Food Safety Laboratory, Beltsville Area Research Center, Agricultural Research Services, United States Department of Agriculture, Beltsville, MD, United States of America</addr-line></nlm:aff></affiliation></author><author><name sortKey="Kim, Seon Woo" sort="Kim, Seon Woo" uniqKey="Kim S" first="Seon Woo" last="Kim">Seon Woo Kim</name><affiliation><nlm:aff id="aff001"><addr-line>Environmental Microbial and Food Safety Laboratory, Beltsville Area Research Center, Agricultural Research Services, United States Department of Agriculture, Beltsville, MD, United States of America</addr-line></nlm:aff></affiliation></author><author><name sortKey="Pettengill, James" sort="Pettengill, James" uniqKey="Pettengill J" first="James" last="Pettengill">James Pettengill</name><affiliation><nlm:aff id="aff002"><addr-line>Office of Analytics and Outreach, Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, College Park, MD, United States of America</addr-line></nlm:aff></affiliation></author><author><name sortKey="Luo, Yan" sort="Luo, Yan" uniqKey="Luo Y" first="Yan" last="Luo">Yan Luo</name><affiliation><nlm:aff id="aff002"><addr-line>Office of Analytics and Outreach, Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, College Park, MD, United States of America</addr-line></nlm:aff></affiliation></author><author><name sortKey="Karns, Jeffrey S" sort="Karns, Jeffrey S" uniqKey="Karns J" first="Jeffrey S." last="Karns">Jeffrey S. Karns</name><affiliation><nlm:aff id="aff001"><addr-line>Environmental Microbial and Food Safety Laboratory, Beltsville Area Research Center, Agricultural Research Services, United States Department of Agriculture, Beltsville, MD, United States of America</addr-line></nlm:aff></affiliation></author><author><name sortKey="Van Kessel, Jo Ann S" sort="Van Kessel, Jo Ann S" uniqKey="Van Kessel J" first="Jo Ann S." last="Van Kessel">Jo Ann S. Van Kessel</name><affiliation><nlm:aff id="aff001"><addr-line>Environmental Microbial and Food Safety Laboratory, Beltsville Area Research Center, Agricultural Research Services, United States Department of Agriculture, Beltsville, MD, United States of America</addr-line></nlm:aff></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><italic>Salmonella enterica</italic> subsp. <italic>enterica</italic> serovar Kentucky is frequently isolated from healthy poultry and dairy cows and is occasionally isolated from people with clinical disease. A genomic analysis of 119 isolates collected in the United States from dairy cows, ground beef, poultry and poultry products, and human clinical cases was conducted. Results of the analysis demonstrated that the majority of poultry and bovine-associated <italic>S</italic>. Kentucky were sequence type (ST) 152. Several bovine-associated (n = 3) and food product isolates (n = 3) collected from the United States and the majority of human clinical isolates were ST198, a sequence type that is frequently isolated from poultry and occasionally from human clinical cases in Northern Africa, Europe and Southeast Asia. A phylogenetic analysis indicated that both STs are more closely related to other <italic>Salmonella</italic> serovars than they are to each other. Additionally, there was strong evidence of an evolutionary divergence between the poultry-associated and bovine-associated ST152 isolates that was due to polymorphisms in four core genome genes. The ST198 isolates recovered from dairy farms in the United States were phylogenetically distinct from those collected from human clinical cases with 66 core genome SNPs differentiating the two groups, but more isolates are needed to determine the significance of this distinction. Identification of <italic>S</italic>. Kentucky ST198 from dairy animals in the United States suggests that the presence of this pathogen should be monitored in food-producing animals.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Foley, Sl" uniqKey="Foley S">SL Foley</name></author><author><name sortKey="Nayak, R" uniqKey="Nayak R">R Nayak</name></author><author><name sortKey="Hanning, Ib" uniqKey="Hanning I">IB Hanning</name></author><author><name sortKey="Johnson, Tj" uniqKey="Johnson T">TJ Johnson</name></author><author><name sortKey="Han, J" uniqKey="Han J">J Han</name></author><author><name sortKey="Ricke, Sc" uniqKey="Ricke S">SC Ricke</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Kessel, Js" uniqKey="Van Kessel J">JS Van Kessel</name></author><author><name sortKey="Karns, Js" uniqKey="Karns J">JS Karns</name></author><author><name sortKey="Wolfgang, Dr" uniqKey="Wolfgang D">DR 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<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, CA USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">27695032</article-id><article-id pub-id-type="pmc">5047448</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0161225</article-id><article-id pub-id-type="publisher-id">PONE-D-15-43222</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Research and Analysis Methods</subject><subj-group><subject>Database and Informatics Methods</subject><subj-group><subject>Biological Databases</subject><subj-group><subject>Genomic Databases</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Computational Biology</subject><subj-group><subject>Genome Analysis</subject><subj-group><subject>Genomic Databases</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Genetics</subject><subj-group><subject>Genomics</subject><subj-group><subject>Genome Analysis</subject><subj-group><subject>Genomic Databases</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Organisms</subject><subj-group><subject>Animals</subject><subj-group><subject>Vertebrates</subject><subj-group><subject>Amniotes</subject><subj-group><subject>Birds</subject><subj-group><subject>Fowl</subject><subj-group><subject>Gamefowl</subject><subj-group><subject>Chickens</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Organisms</subject><subj-group><subject>Animals</subject><subj-group><subject>Vertebrates</subject><subj-group><subject>Amniotes</subject><subj-group><subject>Birds</subject><subj-group><subject>Poultry</subject><subj-group><subject>Chickens</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Agriculture</subject><subj-group><subject>Livestock</subject><subj-group><subject>Poultry</subject><subj-group><subject>Chickens</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Organisms</subject><subj-group><subject>Animals</subject><subj-group><subject>Vertebrates</subject><subj-group><subject>Amniotes</subject><subj-group><subject>Birds</subject><subj-group><subject>Poultry</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Agriculture</subject><subj-group><subject>Livestock</subject><subj-group><subject>Poultry</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Molecular Biology</subject><subj-group><subject>Molecular Biology Techniques</subject><subj-group><subject>Molecular Biology Assays and Analysis Techniques</subject><subj-group><subject>Phylogenetic Analysis</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Research and Analysis Methods</subject><subj-group><subject>Molecular Biology Techniques</subject><subj-group><subject>Molecular Biology Assays and Analysis Techniques</subject><subj-group><subject>Phylogenetic Analysis</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and life sciences</subject><subj-group><subject>Biochemistry</subject><subj-group><subject>Proteins</subject><subj-group><subject>DNA-binding proteins</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>People and places</subject><subj-group><subject>Geographical locations</subject><subj-group><subject>North America</subject><subj-group><subject>United States</subject><subj-group><subject>Kentucky</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>People and places</subject><subj-group><subject>Geographical locations</subject><subj-group><subject>North America</subject><subj-group><subject>United States</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Medicine and Health Sciences</subject><subj-group><subject>Infectious Diseases</subject><subj-group><subject>Bacterial Diseases</subject><subj-group><subject>Salmonella</subject><subj-group><subject>Salmonella Enterica</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Microbiology</subject><subj-group><subject>Medical Microbiology</subject><subj-group><subject>Microbial Pathogens</subject><subj-group><subject>Bacterial Pathogens</subject><subj-group><subject>Salmonella</subject><subj-group><subject>Salmonella Enterica</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Medicine and Health Sciences</subject><subj-group><subject>Pathology and Laboratory Medicine</subject><subj-group><subject>Pathogens</subject><subj-group><subject>Microbial Pathogens</subject><subj-group><subject>Bacterial Pathogens</subject><subj-group><subject>Salmonella</subject><subj-group><subject>Salmonella Enterica</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Organisms</subject><subj-group><subject>Bacteria</subject><subj-group><subject>Enterobacteriaceae</subject><subj-group><subject>Salmonella</subject><subj-group><subject>Salmonella Enterica</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group></article-categories><title-group><article-title>Genomic and Evolutionary Analysis of Two <italic>Salmonella enterica</italic> Serovar Kentucky Sequence Types Isolated from Bovine and Poultry Sources in North America</article-title><alt-title alt-title-type="running-head">Genomic Analysis from <italic>Salmonella</italic> Kentucky Recovered from Cattle and Poultry</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Haley</surname><given-names>Bradd J.</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Kim</surname><given-names>Seon Woo</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Pettengill</surname><given-names>James</given-names></name><xref ref-type="aff" rid="aff002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Luo</surname><given-names>Yan</given-names></name><xref ref-type="aff" rid="aff002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Karns</surname><given-names>Jeffrey S.</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Van Kessel</surname><given-names>Jo Ann S.</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="corresp" rid="cor001">*</xref></contrib></contrib-group><aff id="aff001"><label>1</label><addr-line>Environmental Microbial and Food Safety Laboratory, Beltsville Area Research Center, Agricultural Research Services, United States Department of Agriculture, Beltsville, MD, United States of America</addr-line></aff><aff id="aff002"><label>2</label><addr-line>Office of Analytics and Outreach, Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, College Park, MD, United States of America</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Cloeckaert</surname><given-names>Axel</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>Institut National de la Recherche Agronomique, FRANCE</addr-line></aff><author-notes><fn fn-type="COI-statement" id="coi001"><p><bold>Competing Interests: </bold>The authors have declared that no competing interests exist.</p></fn><fn fn-type="con"><p><list list-type="simple"><list-item><p><bold>Conceived and designed the experiments:</bold> BJH JP YL JSK JVK.</p></list-item><list-item><p><bold>Performed the experiments:</bold> BJH SWK JP YL.</p></list-item><list-item><p><bold>Analyzed the data:</bold> BJH SWK JP JSK JVK.</p></list-item><list-item><p><bold>Contributed reagents/materials/analysis tools:</bold> BJH JP YL JSK JVK.</p></list-item><list-item><p><bold>Wrote the paper:</bold> BJH JP JSK JVK.</p></list-item></list></p></fn><corresp id="cor001">* E-mail: <email>joann.vankessel@ars.usda.gov</email></corresp></author-notes><pub-date pub-type="epub"><day>3</day><month>10</month><year>2016</year></pub-date><pub-date pub-type="collection"><year>2016</year></pub-date><volume>11</volume><issue>10</issue><elocation-id>e0161225</elocation-id><history><date date-type="received"><day>30</day><month>9</month><year>2015</year></date><date date-type="accepted"><day>2</day><month>8</month><year>2016</year></date></history><permissions><license xlink:href="https://creativecommons.org/publicdomain/zero/1.0/"><license-p>This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the <ext-link ext-link-type="uri" xlink:href="https://creativecommons.org/publicdomain/zero/1.0/">Creative Commons CC0</ext-link> public domain dedication.</license-p></license></permissions><self-uri content-type="pdf" xlink:href="pone.0161225.pdf"></self-uri><abstract><p><italic>Salmonella enterica</italic> subsp. <italic>enterica</italic> serovar Kentucky is frequently isolated from healthy poultry and dairy cows and is occasionally isolated from people with clinical disease. A genomic analysis of 119 isolates collected in the United States from dairy cows, ground beef, poultry and poultry products, and human clinical cases was conducted. Results of the analysis demonstrated that the majority of poultry and bovine-associated <italic>S</italic>. Kentucky were sequence type (ST) 152. Several bovine-associated (n = 3) and food product isolates (n = 3) collected from the United States and the majority of human clinical isolates were ST198, a sequence type that is frequently isolated from poultry and occasionally from human clinical cases in Northern Africa, Europe and Southeast Asia. A phylogenetic analysis indicated that both STs are more closely related to other <italic>Salmonella</italic> serovars than they are to each other. Additionally, there was strong evidence of an evolutionary divergence between the poultry-associated and bovine-associated ST152 isolates that was due to polymorphisms in four core genome genes. The ST198 isolates recovered from dairy farms in the United States were phylogenetically distinct from those collected from human clinical cases with 66 core genome SNPs differentiating the two groups, but more isolates are needed to determine the significance of this distinction. Identification of <italic>S</italic>. Kentucky ST198 from dairy animals in the United States suggests that the presence of this pathogen should be monitored in food-producing animals.</p></abstract><funding-group><funding-statement>This project was supported by internal USDA/ARS research funding.</funding-statement></funding-group><counts><fig-count count="12"></fig-count><table-count count="7"></table-count><page-count count="36"></page-count></counts><custom-meta-group><custom-meta id="data-availability"><meta-name>Data Availability</meta-name><meta-value>Accession numbers for genomic data in NCBI are available in the relevant Supporting Information file.</meta-value></custom-meta></custom-meta-group></article-meta><notes><title>Data Availability</title><p>Accession numbers for genomic data in NCBI are available in the relevant Supporting Information file.</p></notes></front><body><sec sec-type="intro" id="sec001"><title>Introduction</title><p><italic>Salmonella enterica</italic> subsp. <italic>enterica</italic> is a major cause of human and animal salmonellosis worldwide. The majority of human salmonellosis in the United States is caused by several serovars, namely Enteritidis, Typhimurium, Newport, and Javiana [<xref rid="pone.0161225.ref001" ref-type="bibr">1</xref>]. Although it is currently assumed that all serovars are potentially pathogenic to humans, the association between <italic>S</italic>. <italic>enterica</italic> and non-human animal illness as well as aymptomatic carriage is not as clear and frequently animals shedding several serovars such as Kentucky, Enteritidis, and Seftenberg are asymptomatic [<xref rid="pone.0161225.ref001" ref-type="bibr">1</xref>][<xref rid="pone.0161225.ref002" ref-type="bibr">2</xref>][<xref rid="pone.0161225.ref003" ref-type="bibr">3</xref>]. <italic>S</italic>. Kentucky is frequently isolated from both asymptomatic cattle, poultry and poultry products in the United States, but has been isolated from other sources such as the environment and domesticated dogs [<xref rid="pone.0161225.ref002" ref-type="bibr">2</xref>][<xref rid="pone.0161225.ref003" ref-type="bibr">3</xref>][<xref rid="pone.0161225.ref004" ref-type="bibr">4</xref>][<xref rid="pone.0161225.ref005" ref-type="bibr">5</xref>][<xref rid="pone.0161225.ref006" ref-type="bibr">6</xref>];;. Recently, the global spread of multi-drug resistant <italic>S</italic>. Kentucky ST198 has been described, indicating this serovar is an emerging public health threat[<xref rid="pone.0161225.ref007" ref-type="bibr">7</xref>]. In North America, human clinical cases of <italic>S</italic>. Kentucky ST198 infections have been associated with travel to the Middle East, Southeast Asia or Africa [<xref rid="pone.0161225.ref007" ref-type="bibr">7</xref>][<xref rid="pone.0161225.ref008" ref-type="bibr">8</xref>][<xref rid="pone.0161225.ref009" ref-type="bibr">9</xref>], and clinical cases caused by <italic>S</italic>. Kentucky ST152 are relatively rare.</p><p><italic>Salmonella</italic> Kentucky has been identified as the most frequently isolated serovar from non-human non-clinical cases in the United States [<xref rid="pone.0161225.ref001" ref-type="bibr">1</xref>] and until recently, has mostly been considered a concern with poultry due to its high prevalence in broilers as well as the established link between poultry products and human salmonellosis. Further, <italic>S</italic>. Kentucky isolated from poultry and poultry products in the United States has been reported to be resistant to multiple antibiotics [<xref rid="pone.0161225.ref004" ref-type="bibr">4</xref>][<xref rid="pone.0161225.ref010" ref-type="bibr">10</xref>][<xref rid="pone.0161225.ref011" ref-type="bibr">11</xref>][<xref rid="pone.0161225.ref012" ref-type="bibr">12</xref>][<xref rid="pone.0161225.ref013" ref-type="bibr">13</xref>]. This serovar is currently the most frequently isolated serovar from poultry in the United States, recently supplanting Enteritidis and Heidelberg in poultry flocks [<xref rid="pone.0161225.ref002" ref-type="bibr">2</xref>]. In recent years, research on dairy farms has demonstrated that <italic>S</italic>. Kentucky is frequently isolated from dairy cows and dairy production operations in the United States and the isolation rates of this serovar in dairy cows appear to be increasing [<xref rid="pone.0161225.ref003" ref-type="bibr">3</xref>][<xref rid="pone.0161225.ref014" ref-type="bibr">14</xref>][<xref rid="pone.0161225.ref015" ref-type="bibr">15</xref>]). What remains unclear is how <italic>S</italic>. Kentucky is capable of colonizing these two distantly related hosts and if there are genomic differences between poultry-associated and bovine-associated <italic>S</italic>. Kentucky.</p><p>Both <italic>S</italic>. Kentucky ST152 and ST198 have been isolated globally, but the degree of relatedness among <italic>S</italic>. Kentucky isolates recovered on a global scale is not yet known. Multi-Locus Sequence Typing (MLST) analyses have demonstrated that there are no common alleles between these two STs, indicating that they are distantly related [<xref rid="pone.0161225.ref007" ref-type="bibr">7</xref>]. However, comprehensive description of the genomic differences between these two STs has not yet been conducted, and a comparison of the two may identify genomic factors involved in host-specificity and virulence potential. In a whole-genome phylogenetic analysis of <italic>S</italic>. <italic>enterica</italic> Timme et al. [<xref rid="pone.0161225.ref016" ref-type="bibr">16</xref>] demonstrated that <italic>S</italic>. Kentucky was polyphyletic; a phenomenon identified in other serovars [<xref rid="pone.0161225.ref017" ref-type="bibr">17</xref>]. For <italic>S</italic>. <italic>enterica</italic> and other bacteria, polyphyly has been associated with lateral gene transfer (LGT) of the antigenic coding regions [<xref rid="pone.0161225.ref017" ref-type="bibr">17</xref>][<xref rid="pone.0161225.ref018" ref-type="bibr">18</xref>]. The historical application of serology to strain naming and description has thus been misleading in that distantly related strains that have acquired an antigenic coding region from the same source through LGT have been presumed to be similar based on this scheme, when in fact their genomes may be highly diverged.</p><p>The objective of this study was to infer the phylogeny of <italic>S</italic>. Kentucky, identify the genomic features associated with the apparent specificity of <italic>S</italic>. Kentucky ST152 to bovine and poultry hosts/products, and to identify differences between <italic>S</italic>. Kentucky ST152 and ST198 genomes. To accomplish this we sequenced the genomes of 49 dairy-cow associated isolates and two poultry-associated isolates that were collected from across the United States, and coupled these data with the genomes of 68 isolates previously collected from poultry, poultry products and production environments and clinical cases in the United States, Canada, and the Middle East.</p></sec><sec sec-type="materials|methods" id="sec002"><title>Materials and Methods</title><p><italic>Salmonella</italic> Kentucky isolates from dairy cows were recovered from previously collected National Animal Health Monitoring System (NAHMS) samples as well as an eight-year dairy farm monitoring program conducted in south-central Pennsylvania [<xref rid="pone.0161225.ref003" ref-type="bibr">3</xref>][<xref rid="pone.0161225.ref019" ref-type="bibr">19</xref>] (two poultry-associated isolates were supplied by S. Parveen). Isolates were serogrouped following the methods of Herrera-Leon et al. [<xref rid="pone.0161225.ref020" ref-type="bibr">20</xref>]and Karns et al. [<xref rid="pone.0161225.ref021" ref-type="bibr">21</xref>]and serotypes were identified by National Veterinary Services Laboratories (NVSL; Ames, IA). Isolates were streaked onto tryptic soy agar, and a single colony was inoculated in tryptic soy broth overnight at 37°C. This inoculum was centrifuged, decanted, and then processed for DNA extraction using a Qiagen DNeasy Kit (Qiagen, Valencia, CA). Nextera XT libraries were made for each sample and pooled into equimolar concentrations following the manufacturer’s instructions (Illumina, San Diego, CA). Paired-end sequencing (2 X 151 bp) was conducted on an Illumina NextSeq 500 sequencing platform with a High-Output flow cell. Data were demultiplexed and trimmed to remove adaptor sequences using the BCL2FastQ program and PhiX reads were removed using DeconSeq [<xref rid="pone.0161225.ref022" ref-type="bibr">22</xref>]. Reads were further cleaned using Trimmomatic [<xref rid="pone.0161225.ref023" ref-type="bibr">23</xref>] and assembled using SPAdes 3.6.2 [<xref rid="pone.0161225.ref024" ref-type="bibr">24</xref>]. ST56 complex genomes were downloaded from NCBI GenBank prior to February 2016 and ST15 complex genomes were downloaded prior to December 2015 (PRJNA242614, PRJNA273513, PRJNA78335, PRJNA78339, PRJNA78337, PRJNA225734, PRJNA225734, PRJNA66693, PRJNA20069, PRJNA19457, PRJEB6491, PRJNA337914, and PRJNA186035) (<xref ref-type="supplementary-material" rid="pone.0161225.s001">S1 Table</xref>). Genomes from BioProject PRJEB6491 [<xref rid="pone.0161225.ref025" ref-type="bibr">25</xref>] were labeled as 915c and 917c in this analysis. Bovine-associated <italic>S</italic>. Kentucky isolates used in this study will be provided upon request.</p><p>Three methods were used to identify SNPs among the <italic>S</italic>. Kentucky genomes. To identify high quality SNPs based on read coverage, Lyve-SET [<xref rid="pone.0161225.ref026" ref-type="bibr">26</xref>] [<xref rid="pone.0161225.ref027" ref-type="bibr">27</xref>]and CFSAN SNP Pipeline [<xref rid="pone.0161225.ref028" ref-type="bibr">28</xref>] [<xref rid="pone.0161225.ref029" ref-type="bibr">29</xref>] were used. For the Lyve-SET analysis SNP identification was conducted with a minimum 10X coverage requirement and the CGP read cleaner. When fastq files were not available, assembled genomes were included in the project/asm directory prior to SNP detection. Default settings were used for the CFSAN SNP Pipeline analysis. For this analysis only genomes for which fastq files or.sff files were available could be used for the SNP search and therefore one ST152 (strain CDC 191) genome and several ST198 genomes were excluded (strains CVM 43824, CVM 43780, CVM 43756). To identify SNPs in assembled genomes, Parsnp from the Harvest package was used [<xref rid="pone.0161225.ref030" ref-type="bibr">30</xref>][<xref rid="pone.0161225.ref031" ref-type="bibr">31</xref>]. Regions undergoing high levels of recombination were removed using the–x option (PhiPack) and all genomes were included using the–c option. The chromosome of <italic>S</italic>. Kentucky CVM21988 was selected as the reference genome for all analyses. Parsnp was used to determine the core-genome SNP differences between <italic>S</italic>. Kentucky ST152 and ST198 genomes and other subclade A1 serovars identified by Timme et al. [<xref rid="pone.0161225.ref016" ref-type="bibr">16</xref>]. SNPs were annotated using snpEff [<xref rid="pone.0161225.ref032" ref-type="bibr">32</xref>].</p><p>To infer the phylogeny of <italic>S</italic>. Kentucky ST152/318/2132 isolates the multi-fasta SNP matrices from each of the three SNP detection methods were imported into MEGA6 [<xref rid="pone.0161225.ref033" ref-type="bibr">33</xref>] and RAxML [<xref rid="pone.0161225.ref034" ref-type="bibr">34</xref>]. Maximum Parsimony (MP) trees were inferred for all <italic>S</italic>. Kentucky isolates (ST198, ST152/318/2132, and unknown STs) as well as <italic>S</italic>. Kentucky isolates with non-<italic>S</italic>. Kentucky isolates using MEGA6. For phylogenetic inference of ST152/318/2132 isolates a Maximum Likelihood phylogeny was inferred using RAxML version 8.2.8 with the General Time Reversible (GTR) selected as the model of nucleotide substitution and all other parameters set to default settings. MP and ML analyses were each conducted with 1000 bootstrap replicates. The bootstrapped MP tree inferred from the Parsnp method of SNP detection was used to determine the genealogical sorting index (gsi) [<xref rid="pone.0161225.ref035" ref-type="bibr">35</xref>] for bovine and poultry isolates using the GSI webserver [<xref rid="pone.0161225.ref036" ref-type="bibr">36</xref>]. MLST data were retrieved from the University of Warwick <italic>Salmonella enterica</italic> MLST database [<xref rid="pone.0161225.ref006" ref-type="bibr">6</xref>]and through the Center for Genomic Epidemiology server [<xref rid="pone.0161225.ref037" ref-type="bibr">37</xref>].</p><p>The resulting SNP matrices were also analyzed using the Bayesian clustering program STRUCTURE v2.3.4 [<xref rid="pone.0161225.ref038" ref-type="bibr">38</xref>]. This program clusters individuals based on patterns of SNP differences without enforcing a bifurcating tree-like structure and, thus, may reveal additional details about the genomic content, similarity, and differences among isolates. In particular, STRUCTURE is suitable for detecting evidence for admixture (i.e., individuals whose genomes appear to be a combination of SNPs associated with distinct groups). STRUCTURE analyses were run with default settings and 60,000 generations, the first 10,000 of which served as the burnin. STRUCTURE results were visualized using DISTRUCT v1.1 [<xref rid="pone.0161225.ref039" ref-type="bibr">39</xref>].</p><p>Putative plasmid sequences were identified with PlasmidFinder [<xref rid="pone.0161225.ref040" ref-type="bibr">40</xref>] using the <italic>Enterobacteriaceae</italic> database with the detection thresholds set to 95% sequence identity. The identified HSP fragments were compared to the NCBI database (BLASTN analysis) to identify similar plasmids. The presence/absence of protein coding genes was conducted using BLASTP. Putative genomic islands in <italic>S</italic>. Kentucky CVM29188 (ST152) and <italic>S</italic>. Kentucky CVM N51290 (ST198) were identified using IslandViewer 3 [<xref rid="pone.0161225.ref041" ref-type="bibr">41</xref>].</p><p>Forty nine bovine-associated <italic>S</italic>. Kentucky isolates, and two poultry-associated isolates (including type strain ATCC 9263) were evaluated for their susceptibility to 25 antimicrobials (azithromycin, ciprofloxacin, gentamicin, tetracycline, nalidixic acid, cefoxitin, chloramphenicol, ceftriaxone, amoxicillin/clavulanic acid, ceftiofur, sulfisoxazole, trimethoprim/sulfamethoxazole, ampicillin, streptomycin, cephalothin, cefotaxime, cefotaxime/clavulanic acid, ceftazidime, ceftazidime/clavulanic acid, imipenem, cefepime, cefpodoxime, piperacillin/tazobactam, meropenem, and cefazolin) using an automated microdilution procedure (Sensititre, ThermoFisher, Lenexa, KS) and specialty plates CVM3AGNF and ESB1F. Antimicrobial minimum inhibitory concentrations (MICs) were interpreted based upon the epidemiological cut-off values (ECOFFs) used by the National Antimicrobial Resistance Monitoring System (NARMS).</p></sec><sec id="sec003"><title>Results and Discussion</title><sec id="sec004"><title>Description of Isolates and Antibiotic Susceptibility Testing of Bovine-associated Isolates</title><p>In total 119 <italic>S</italic>. Kentucky genomes were utilized in the <italic>in silico</italic> analyses of this study. Of these, 49 dairy cow-associated isolates were selected from a collection of isolates that have been recovered from routine analysis of milk, milk filters, fecal samples from dairy cows, and the dairy cow farm environment in the United States over a 13-year period (<xref ref-type="table" rid="pone.0161225.t001">Table 1</xref>). Two poultry isolates recovered from a broiler operation in the Eastern Shore of Maryland were used in this study, and the remaining poultry-associated genomes (n = 59) were gathered as assembled genomes and raw sequencing reads from the NCBI GenBank database and the SRA database, respectively. All human clinical isolates (n = 6) were all gathered from NCBI (two as raw sequencing reads and four as assembled genomes).</p><table-wrap id="pone.0161225.t001" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0161225.t001</object-id><label>Table 1</label><caption><title>Kentucky genomes used in this study.</title><p><italic>S</italic>.</p></caption><alternatives><graphic id="pone.0161225.t001g" xlink:href="pone.0161225.t001"></graphic><table frame="hsides" rules="groups"><colgroup span="1"><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col></colgroup><thead><tr><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Genome</th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Source</th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Location of Isolation</th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Year of Isolation</th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Sequence Type (ST)<xref ref-type="table-fn" rid="t001fn001"><sup>1</sup></xref></th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">ST Complex <xref ref-type="table-fn" rid="t001fn002"><sup>2</sup></xref></th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Phylogenetic Cluster <xref ref-type="table-fn" rid="t001fn003"><sup>3</sup></xref></th></tr></thead><tbody><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC97</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Fecal composite (dairy cow farm)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pennsylvania (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2004</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">318</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">0253</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Dairy cow feces</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pennsylvania (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2004</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">318</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">5349</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Dairy cow feces</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pennsylvania (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2006</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC444</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Milk filter (dairy cow farm)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Wisconsin (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2007</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC457</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Milk filter (dairy cow farm)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Texas (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2007</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC515</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Bulk tank milk (dairy cow farm)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Wisconsin (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2007</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC521</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Milk filter (dairy cow farm)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Wisconsin (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2007</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC572</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Milk filter (dairy cow farm)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Wisconsin (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2007</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC913</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Dairy cow feces</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Virginia (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2007</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC526</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Bulk tank milk (dairy cow farm)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pennsylvania (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2007</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC496</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Milk filter (dairy cow farm)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">New York (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2007</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CFSAN011775</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Dairy cow feces</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pennsylvania (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2008</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CFSAN011776</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Dairy cow feces</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pennsylvania (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2009</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CFSAN011777</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Dairy cow feces</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pennsylvania (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2009</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CFSAN011778</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Dairy cow feces</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pennsylvania (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2009</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CFSAN011779</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Dairy cow feces</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pennsylvania (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2010</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CFSAN011780</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Dairy cow feces</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pennsylvania (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2010</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CFSAN011782</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Dairy cow feces</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pennsylvania (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2011</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC6181</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Milk filter (dairy cow farm)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pennsylvania (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2011</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC6183</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Milk filter (dairy cow farm)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pennsylvania (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2011</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC6204</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Milk filter (dairy cow farm)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pennsylvania (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2011</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC6329</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Milk filter (dairy cow farm)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pennsylvania (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2011</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC6333</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Milk filter (dairy cow farm)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pennsylvania (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2011</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC6340</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Milk filter (dairy cow farm)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pennsylvania (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2011</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC8561</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Dairy cow feces</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pennsylvania (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2011</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC8574</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Fecal composite (dairy cow farm)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pennsylvania (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2011</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC8601</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Dairy cow feces</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pennsylvania (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2011</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC8619</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Dairy cow feces</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pennsylvania (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2011</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC8624</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Dairy cow feces</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pennsylvania (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2011</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC8625</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Dairy cow feces</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pennsylvania (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2011</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC8633</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Fecal composite (dairy cow farm)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pennsylvania (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2011</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CFSAN011781</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Dairy cow feces</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pennsylvania (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2012</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC7487</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Dairy cow hide swab</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pennsylvania (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC8078</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Fecal composite (dairy cow farm)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pennsylvania (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC8289</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Milk filter (dairy cow farm)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ohio (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2014</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC8294</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Milk filter (dairy cow farm)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pennsylvania (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2014</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC8297</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Milk filter (dairy cow farm)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ohio (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2014</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC8417</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Trough water (dairy cow farm)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pennsylvania (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2014</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC8446</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Milk filter (dairy cow farm)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ohio (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2014</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1.2.3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC353</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Milk filter (dairy cow farm)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">New York (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2007</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC469</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Bulk tank milk (dairy cow farm)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Vermont (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2007</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC661</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Milk filter (dairy cow farm)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Vermont (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2007</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC690</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Milk filter (dairy cow farm)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Wisconsin (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2007</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC912</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Dairy cow feces</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Wisconsin (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2007</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC917</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Dairy cow feces</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Vermont (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2007</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CDC 191</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Human clinical</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Wisconsin (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2002</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC621</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Milk filter (dairy cow farm)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Texas (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2007</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N50435</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">New Mexico (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.3.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N51252</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken wings</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Colorado (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.3.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N51981</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Louisiana (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.3.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N43447</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">California (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.3.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">13562</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Iowa (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2001</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.3.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N43450</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">California (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.3.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N43478</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Oregon (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.3.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N43820</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Georgia (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.3.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">SA20030505</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken cecal contents</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ontario (Canada)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2002</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.3.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ABBSB1008-2</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken feces</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">British Columbia (Canada)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2006</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.3.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N45412</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Washington (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.3.3.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N47718</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">New Mexico (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.3.3.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N47730</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">New Mexico (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.3.3.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N51241</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">California (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.3.3.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N47729</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Washington (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">unknown</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.3.3.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N45934</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Maryland (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.3.3.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N45937</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Maryland (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.3.3.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N45939</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Maryland (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.3.3.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N45944</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Maryland (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.3.3.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N46849</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pennsylvania (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.3.3.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N47721</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">New Mexico (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.3.3.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N47723</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ground turkey</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">New Mexico (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.3.3.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N48688</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Maryland (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.3.3.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N48710</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Tennessee (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.3.3.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N47722</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ground turkey</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">New Mexico (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.3.3.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N48687</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Maryland (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.3.3.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N48705</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Louisiana (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.3.3.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N44693</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken wings</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">California (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.3.3.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N43448</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken wings</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">California (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.4.1.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N43455</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Colorado (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.4.1.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N43835</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Washington (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.4.1.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N46820</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Colorado (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.4.1.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N46857</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pennsylvania (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.4.1.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N50419</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Connecticut (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.4.1.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N50421</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken wings</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Georgia (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.4.1.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N51294</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">New Mexico (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.4.1.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N51313</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Tennessee (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.4.1.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ABB07-SB3057-2</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken feces</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">British Columbia (Canada)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2005</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.4.1.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ABB1087-1</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken feces</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">British Columbia (Canada)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2005</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.4.1.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">22694</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Massachusetts (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2002</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.4.1.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N51982</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken wings</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Louisiana (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.4.1.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM29188</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Georgia (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2003</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.4.1.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N43465</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Minnesota (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.4.1.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N43466</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken wings</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Minnesota (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.4.1.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N44708</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">New York (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.4.1.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N47720</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">New Mexico (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.4.1.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N48707</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">New York (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.4.1.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N48711</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Tennessee (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.4.1.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N50437</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken wings</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">New York (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.4.1.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N51256</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken wings</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Connecticut (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.4.1.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N51273</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Maryland (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.4.1.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC5795</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Poultry</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Maryland (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2010</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.4.1.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC5805</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Poultry</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Maryland (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2010</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.4.1.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N50429</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">New Mexico (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.4.1.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N51277</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Maryland (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.4.1.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N51249</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Colorado (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2132</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.4.1.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N43471</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">New Mexico (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.4.1.3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">SALC-205-3</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken feces</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">British Columbia (Canada)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2004</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.4.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">20793</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Georgia (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2002</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.4.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">N312</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Chicken breast</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Minnesota (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2004</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2.4.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC938</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Milk filter (dairy cow farm)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Florida (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2003</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">198</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">56</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">198.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ATCC 9263</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">unknown</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">unknown</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Unknown</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">198</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">56</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">198.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N51290</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ground beef</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">New Mexico (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2013</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">198</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">56</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">198.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC273</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Dairy calf feces</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Florida (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2011</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">198</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">56</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">198.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC274</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Dairy calf feces</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Florida (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2011</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">198</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">56</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">198.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N41913</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ground beef</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Minnesota (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2012</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">198</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">56</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">198.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N42453</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ground turkey</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">California (USA)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2012</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">unknown</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">56</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">198.1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">915c</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Human clinical (sacral wound)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Kuwait</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2012</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">198</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">56</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">198.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">917c</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Human clinical (stool)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Kuwait</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2012</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">198</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">56</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">198.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM 43824</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Human clinical</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">unknown</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2012</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">198</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">56</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">198.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM 43780</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Human clinical</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">unknown</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2011</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">198</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">56</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">198.2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM 43756</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Human clinical</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">unknown</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2012</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">198</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">56</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">198.2</td></tr></tbody></table></alternatives><table-wrap-foot><fn id="t001fn001"><p><sup>1</sup> as determined by the Center for Genomic Epidemiology web server [<xref rid="pone.0161225.ref037" ref-type="bibr">37</xref>].</p></fn><fn id="t001fn002"><p><sup>2</sup> determined by the University of Warwick MLST database [<xref rid="pone.0161225.ref006" ref-type="bibr">6</xref>]. ST complex assignment was given to unknown ST based on their similarity to known ST.</p></fn><fn id="t001fn003"><p><sup>3</sup> based on MP tree inferred from SNPs identified by Parsnp (See <xref ref-type="fig" rid="pone.0161225.g002">Fig 2</xref>).</p></fn></table-wrap-foot></table-wrap><p>For in-house isolates (bovine-associated isolates and two poultry isolates, and ATCC 9263) antibiotic susceptibility tests (AST) were conducted. All bovine-associated isolates and ATCC 9263 were susceptible to all tested antibiotics. Poultry isolate ARS-CC5795 was resistant to amoxicillin/clauvulanic acid, ampicillin, cefitoxin, ceftiofur, and ceftriaxone, while ARS-CC5805 (poultry isolate) was resistant to streptomycin and tetracycline. The absence of antibiotic resistance in a diverse collection of <italic>S</italic>. Kentucky recovered from dairy cows is consistent with other studies and is notable due to the reported prevalence of antibiotic resistance among poultry-associated <italic>S</italic>. Kentucky within the United States [<xref rid="pone.0161225.ref004" ref-type="bibr">4</xref>][<xref rid="pone.0161225.ref010" ref-type="bibr">10</xref>][<xref rid="pone.0161225.ref011" ref-type="bibr">11</xref>][<xref rid="pone.0161225.ref012" ref-type="bibr">12</xref>][<xref rid="pone.0161225.ref013" ref-type="bibr">13</xref>][<xref rid="pone.0161225.ref042" ref-type="bibr">42</xref>][<xref rid="pone.0161225.ref043" ref-type="bibr">43</xref>]. Antibiotic resistance conferring plasmids are frequently identified in poultry-associated <italic>S</italic>. Kentucky ST152 isolates [<xref rid="pone.0161225.ref012" ref-type="bibr">12</xref>] while antibiotic resistance in human clinical S. Kentucky ST198 is associated with acquisition of the <italic>Salmonella</italic> Genomic Island 1 (SGI1), plasmids, and core genome polymorphisms [<xref rid="pone.0161225.ref007" ref-type="bibr">7</xref>][<xref rid="pone.0161225.ref009" ref-type="bibr">9</xref>][<xref rid="pone.0161225.ref044" ref-type="bibr">44</xref>][<xref rid="pone.0161225.ref045" ref-type="bibr">45</xref>].</p></sec><sec id="sec005"><title>Phylogenetic Inference</title><p>To date at least 15 <italic>S</italic>. Kentucky sequence types (ST) have been described MLST studies [<xref rid="pone.0161225.ref007" ref-type="bibr">7</xref>][<xref rid="pone.0161225.ref046" ref-type="bibr">46</xref>]. In these assays, each ST differs from another ST by at least one of seven alleles with some sequence types of the same serovar sharing no alleles. Based on the number of deposited sequences in the MLST database, ST152 and ST198 are among the most frequently isolated <italic>S</italic>. Kentucky sequence types. However, they share no common alleles indicating they are highly diverged from each other [<xref rid="pone.0161225.ref007" ref-type="bibr">7</xref>]. These two STs are part of two larger groups of “ST complexes” which consist of closely related STs. ST152 is a member of the ST15 complex which to date also includes ST151, 212, 318, and 723 among several others. ST198 is a member of ST56 complex, which also includes ST727, 835, and 1680. Outside of these defined complexes there are other closely related STs sharing one or more alleles with members of each complex. A pairwise comparison of seven MLST loci of ST152 and ST198 identified 42 SNPs resulting in a 1.25% sequence divergence (data not shown). When applying the same MLST criteria to the <italic>S</italic>. Kentucky genomes used in this study, three STs were identified (<xref ref-type="table" rid="pone.0161225.t001">Table 1</xref>). All of the poultry-associated isolates were ST152 with one identified as 2132 (one allele difference with ST152), while two bovine-associated isolates were ST318 (one allele difference with ST152 and ST2132), 42 were ST152, and three were ST198. Two strains could not be accurately typed due to abbreviated genes at the ends of contigs. <italic>S</italic>. Kentucky CVM N47729, isolated from a chicken breast, shared six of seven alleles with ST152 and <italic>S</italic>. Kentucky CVM N42453 shared six of seven alleles with ST198. Eight <italic>S</italic>. Kentucky genomes gathered from the NCBI database were identified as ST198. These included five human clinical isolates, two ground beef isolates, and the <italic>S</italic>. Kentucky type strain ATCC 9263. These results are consistent with those of the MLST database in that ST152 from the United States are commonly isolated from poultry and cattle. A single ST318 isolate is included in the MLST database and, consistent with our study, was isolated from cattle in the mid-Atlantic region of the United States. Based on data entries in the MLST database ST198 isolates were recovered on an apparently broader global scale and were more frequently associated with human infections and poultry, cattle, and occasionally other organisms, and the environment.</p><p>To investigate the relatedness among the <italic>S</italic>. Kentucky isolates and infer their evolutionary history on a genome-wide scale, SNPs across the genomes of all <italic>S</italic>. Kentucky and closely related serovars were identified. Using a core-genome SNP matrix determined from representative genomes of <italic>S</italic>. <italic>enterica</italic> subclade A1 as described by Timme et al. [<xref rid="pone.0161225.ref016" ref-type="bibr">16</xref>], two distant lineages of <italic>S</italic>. Kentucky were identified (<xref ref-type="fig" rid="pone.0161225.g001">Fig 1</xref>). These two <italic>S</italic>. Kentucky lineages correspond to the ST15 complex (ST152, 318 and 2132) and ST56 complex (ST198) of <italic>S</italic>. Kentucky sequence types described above (<xref ref-type="table" rid="pone.0161225.t001">Table 1</xref>).</p><fig id="pone.0161225.g001" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0161225.g001</object-id><label>Fig 1</label><caption><title>Phylogenetic relationships of <italic>S</italic>. Kentucky ST152 and ST198 with representatives of subclade A1 serovars as described by Timme et al.</title><p>[<xref rid="pone.0161225.ref016" ref-type="bibr">16</xref>] inferred using the Maximum Likelihood method with the General Time Reversible model of nucleotide substitution. Bar length represents number of substitutions per site.</p></caption><graphic xlink:href="pone.0161225.g001"></graphic></fig><p>SNPs within the <italic>S</italic>. Kentucky genomes (excluding other subclade A1 serovars) were identified using three methods and phylogenetic trees were inferred from these data. Phylogenies inferred from data derived from the three SNP-detection methods were, for the most part, approximately similar in topology. For all SNP detection methods and both methods of tree inference the ST15 complex was observed to have two major sublineages (labeled as Lineages 1.0 and 2.0 in this analysis) (Figs <xref ref-type="fig" rid="pone.0161225.g002">2</xref>–<xref ref-type="fig" rid="pone.0161225.g007">7</xref>). For both MP and ML analyses there was weak support for the placement of some sublineages in the ancestral node of the Lineage 1.0 and for the Parsnp ML analysis the human clinical isolate (<italic>S</italic>. Kentucky CDC 191) is rooted in the ancestral node of the tree while it is placed in Lineage 2.0 in the other phylogenetic analyses of this study (Figs <xref ref-type="fig" rid="pone.0161225.g002">2</xref>, <xref ref-type="fig" rid="pone.0161225.g004">4</xref>, and <xref ref-type="fig" rid="pone.0161225.g005">5</xref>).</p><fig id="pone.0161225.g002" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0161225.g002</object-id><label>Fig 2</label><caption><title>Maximum Parsimony tree of <italic>S</italic>. Kentucky ST152/318/2132 genomes based on 40,795 SNPs detected by Parsnp and rooted in twelve <italic>S</italic>. Kentucky ST198 isolates.</title><p>Bovine-associated strains are labeled in blue, poultry-associated strains are labeled in red, and the human clinical isolate CDC 191 is labeled in black. Clusters of genomes are labeled on right of tree. Tree was inferred with 1000 bootstrap replicates. Bar length = number of substitutions.</p></caption><graphic xlink:href="pone.0161225.g002"></graphic></fig><fig id="pone.0161225.g003" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0161225.g003</object-id><label>Fig 3</label><caption><title>Maximum Likelihood tree of <italic>S</italic>. Kentucky ST152/318/2132 genomes based on SNPs detected by Parsnp.</title><p>Bovine-associated strains are labeled in blue, poultry-associated strains are labeled in red, and the human clinical isolate CDC 191 is labeled in black. Tree was inferred using the General Time Reversible model of nucleotide substitution in RAxML with 1000 bootstrap replicates and rooted in twelve <italic>S</italic>. Kentucky ST198 genomes. Bar = number of substitutions per site.</p></caption><graphic xlink:href="pone.0161225.g003"></graphic></fig><fig id="pone.0161225.g004" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0161225.g004</object-id><label>Fig 4</label><caption><title>Maximum Parsimony tree of <italic>S</italic>. Kentucky ST152/318/2132 genomes based on 45,999 SNPs detected by Lyve-SET and rooted in twelve <italic>S</italic>. Kentucky ST198 genomes.</title><p>Bovine-associated strains are labeled in blue, poultry-associated strains are labeled in red, and the human clinical isolate CDC 191 is labeled in black. Tree was inferred with 1000 bootstrap replicates. Bar length = number of substitutions.</p></caption><graphic xlink:href="pone.0161225.g004"></graphic></fig><fig id="pone.0161225.g005" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0161225.g005</object-id><label>Fig 5</label><caption><title>Maximum Likelihood tree of <italic>S</italic>. Kentucky ST152/318/2132 genomes based on SNPs detected by Lyve-SET.</title><p>Bovine-associated strains are labeled in blue, poultry-associated strains are labeled in red, and the human clinical isolate CDC 191 is labeled in black. Tree was inferred using the General Time Reversible model of nucleotide substitution in RAxML with 1000 bootstrap replicates and rooted in twelve <italic>S</italic>. Kentucky ST198 genomes. Bar = number of substitutions per site.</p></caption><graphic xlink:href="pone.0161225.g005"></graphic></fig><fig id="pone.0161225.g006" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0161225.g006</object-id><label>Fig 6</label><caption><title>Maximum Parsimony tree of <italic>S</italic>. Kentucky ST152/318/2132 genomes based on 48,269 SNPs detected by CFSAN SNP Pipeline and rooted in seven <italic>S</italic>. Kentucky ST198 isolates.</title><p>Bovine-associated strains are labeled in blue and poultry-associated strains are labeled in red. Tree was inferred with 1000 bootstrap replicates. Bar length = number of substitutions.</p></caption><graphic xlink:href="pone.0161225.g006"></graphic></fig><fig id="pone.0161225.g007" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0161225.g007</object-id><label>Fig 7</label><caption><title>Maximum Likelihood tree of <italic>S</italic>. Kentucky ST152/318/2132 genomes based on SNPs detected by CFSAN SNP Pipeline.</title><p>Bovine-associated strains are labeled in blue and poultry-associated strains are labeled in red. Tree was inferred using the General Time Reversible model of nucleotide substitution in RAxML with 1000 bootstrap replicates and rooted in seven <italic>S</italic>. Kentucky ST198 genomes. Bar = number of substitutions per site.</p></caption><graphic xlink:href="pone.0161225.g007"></graphic></fig><p>For five of the six analyses Lineage 1.0 is composed to several clusters of bovine isolates (n = 39) labeled as clusters 1.1, 1.2.1, 1.2.2 and 1.2.3. Cluster 1.1 consists of five ST152 isolates, one recovered from Texas and four from Wisconsin in 2007. The Wisconsin isolates grouped together, separately from the single Texas isolate. Cluster 1.2.1 consisted of one isolate from Pennsylvania and one from Virginia. Cluster 1.2.2 consisted of three isolates, two of them were typed as ST318 and collected from the same farm in Pennsylvania while the third isolate was a highly diverged ST152 isolate collected from Ohio. Cluster 1.2.3 consisted of 29 isolates, mainly from Pennsylvania with one isolate from New York, and two from Ohio. These results, however, are not consistent across all tree inference methods as the ML analysis of the SNPs detected with Parsnp place all bovine isolates except ARS-CC621 in Lineage 1.0 (<xref ref-type="fig" rid="pone.0161225.g003">Fig 3</xref>), albeit with low bootstrap support, and consistent with other methods place all poultry isolates in Lineage 2.0.</p><p>The topology of Lineage 2.0 is moderately to strongly supported for all analyses and is composed of three to four clusters of strains. For the Parsnp MP and Lyve-SET and CFSAN SNP Pipeline MP and ML analyses the ancestral Lineage 2.0 node moderately to strongly supported (80 to 94%) with much stronger support for the node from which the bovine-associated isolate ARS-CC621 and all poultry-associated isolates descended for all analyses.</p><p>For all analyses excluding the Parsnp ML analysis the Lineage 2.0 clusters included one group of bovine/human-associated genomes, (cluster 2.1), one bovine-associated genome (cluster 2.2) and two groups of poultry-associated genomes (clusters 2.3.1, 2.3.2, 2.3.3.1, 2.3.3.2 and 2.4.1.1, 2.4.1.2, 2.4.1.3, and 2.4.2) which is consistent with the two dominant poultry-associated pulsotypes described by Ladely et al. [<xref rid="pone.0161225.ref013" ref-type="bibr">13</xref>]. Cluster 2.1 consists of seven isolates; three from Vermont, one from New York two from Wisconsin, and a distantly related human clinical isolate (CDC 191) from Wisconsin in 2002. Within this group the Vermont isolates are more closely related to each other than they are to the New York and Wisconsin isolates, and the Wisconsin isolates are more closely related to each other than they are to the New York or Vermont isolates. Cluster 2.2 consists of a single bovine-associated isolate (ARS-CC621) recovered in Texas in 2007.</p><p>Clusters 2.3.1 to 2.4.2 consist solely of isolates recovered from poultry, poultry products or processing plants and chicken feces/cecum samples collected across North America over a 12 year period. These clusters are monophyletic (geneaological sorting index = 1, P <0.0001) and do not include any bovine isolates indicating complete lineage sorting from the most recent common ancestor that gave rise to poultry-associated isolates. All poultry-associated clusters were moderately to strongly supported for all MP analyses, while ML analyses resulted in lower bootstrap support for clusters 2.4.1.1 to 2.4.2. Within the latter tree, the topologies of cluster 2.4.1 and 2.4.2 are not consistent with those of all other MP and ML trees.</p><p>Within the paraphyletic bovine-associated groups presence of different clusters in the same region (Wisconsin, Texas, and Pennsylvania) indicates multiple evolutionary sublineages of <italic>S</italic>. Kentucky ST152 are circulating in dairy cow herds of the same region. For example, clusters 1.1 and 2.1 isolates were recovered from dairy cows in Wisconsin, cluster 1.1 and 2.2 isolates were recovered from Texas, and clusters 1.2.1, 1.2.2 and 1.2.3 isolates were detected in Pennsylvania. Thus, broad geographic dispersal of <italic>S</italic>. Kentucky strains may have occurred repeatedly over time. Dairy farms are open environments in that there is a potential of wildlife intrusions on the farm and interactions with dairy cows [<xref rid="pone.0161225.ref047" ref-type="bibr">47</xref>]. For example, migratory birds are known vehicles of enteric bacteria and have been suggested to be a transmission route to livestock [<xref rid="pone.0161225.ref048" ref-type="bibr">48</xref>] and some wild bird species such as European starlings (<italic>Sturnus vulgaris</italic>) are known to concentrate in areas of cattle operations where their presence has been associated with increased <italic>S</italic>. <italic>enterica</italic> contamination of feed and water [<xref rid="pone.0161225.ref049" ref-type="bibr">49</xref>].</p><p>Within the poultry-associated clusters, a clear geographic pattern of distribution was not observed. For example, isolates from New Mexico, Tennessee, California, and British Columbia were identified in both clusters 2.3 and 2.4. Along with the presence of isolates from the same state in multiple clusters, geographically disparate isolates grouped together as well. For instance, some isolates from British Columbia (ABBSB1008-2) are sister taxa on the same phylogenetic sublineage with one from Georgia (CVM N43820), two regions that are separated by over 4500 km. Similarly, isolates from California also clustered with those from Tennessee, Massachusetts, New York, Colorado, and Washington State in cluster 2.4. This lack of geographic clustering among the poultry isolates is most likely due to the fact that the majority of these isolates were not collected on-farm, but rather after poultry product processing. Sequencing of isolates collected from farms would help identify any possible geographic signatures present in the currently circulating ST152 populations.</p><p>Based on the genomes analyzed in this study there is strong evidence to support the hypothesis that bovine-associated ST152 isolates are phylogenetically distinct from poultry-associated ST152 isolates. However, these data only reflect those <italic>S</italic>. Kentucky ST152 isolates collected in North America in recent years. It is known that ST152 isolates have been recovered from various sources worldwide and as far back as the 1950s, but what is not well documented in the literature is how frequently ST152 are recovered from cattle and poultry in other continents, or if this ST152-Bovine/Poultry relationship occurs in other regions globally, i.e, if the ST152 strains have entered bovine and/or poultry populations outside of North America or if they are circulating through the populations of other animals. At present, the metadata of only five ST152 strains from outside of North America are deposited in the MLST database and none of these were recovered from poultry or bovine sources, but rather fish meal and human clinical cases. Addition of other ST152 genomes from a variety of sources may result in a somewhat different phylogenetic relationship among strains than what is presented here, as well as the presence of other evolutionary lineages not detected in this study.</p></sec><sec id="sec006"><title>Genomic Polymorphisms within the ST152/ST318/2132 Isolates (ST complex 15)</title><p>In total there were 2662 SNPs identified in the core genome analysis by Parsnp, and 3353 and 3336 identified by Lyve-SET and CFSAN SNP Pipeline, respectively. We further identified Lineage and host-associated SNPs. The Lineage 1.0/2.0 divergence event for all trees excluding the Parsnp ML tree is marked by eight identified SNPs; four in intergenic regions and four in protein coding genes resulting in three synonymous mutations and one non-synonymous mutation (SeKA_A1002, SeKA_A1027, SeKA_A1948, SeKA_A4700) (<xref ref-type="table" rid="pone.0161225.t002">Table 2</xref>).</p><table-wrap id="pone.0161225.t002" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0161225.t002</object-id><label>Table 2</label><caption><title>Identified SNPs defining the Lineage 1.0/2.0 divergence (Top) and the Bovine-associated/Poultry-associated divergence (Bottom).</title><p>S = synonymous mutation. NS = non-synonymous mutation.</p></caption><alternatives><graphic id="pone.0161225.t002g" xlink:href="pone.0161225.t002"></graphic><table frame="hsides" rules="groups"><colgroup span="1"><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col></colgroup><tbody><tr><td align="center" rowspan="9" style="background-color:#FFFFFF" colspan="1">Lineage 1.0 / 2.0</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Locus Tag <xref ref-type="table-fn" rid="t002fn001"><sup>a</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Annotation <xref ref-type="table-fn" rid="t002fn002"><sup>b</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Genome Position <xref ref-type="table-fn" rid="t002fn001"><sup>a</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">nt Lineage 2.0</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">nt Lineage 1.0</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Substitution <xref ref-type="table-fn" rid="t002fn003"><sup>c</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">aa Lineage 2.0</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">aa Lineage 1.0</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">aa Position <xref ref-type="table-fn" rid="t002fn001"><sup>a</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Method</td></tr><tr><td align="left" colspan="2" style="background-color:#FFFFFF" rowspan="1">intergenic</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">528068</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" colspan="4" style="background-color:#FFFFFF" rowspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1, 2, 3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A1002</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">LysR substrate binding domain protein</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">953413</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">S</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Leu</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Leu</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">223</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1, 2, 3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A1027</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">nitrite extrusion protein 2</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">984718</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">S</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pro</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pro</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">288</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1, 2, 3</td></tr><tr><td align="left" colspan="2" style="background-color:#FFFFFF" rowspan="1">intergenic</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1032798</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" colspan="4" style="background-color:#FFFFFF" rowspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2, 3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A1948</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">amidophosphoribosyltransferase</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1876321</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">S</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ala</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ala</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">288</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1, 2, 3</td></tr><tr><td align="left" colspan="2" style="background-color:#FFFFFF" rowspan="1">intergenic</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2432705</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" colspan="4" style="background-color:#FFFFFF" rowspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1, 2, 3</td></tr><tr><td align="left" colspan="2" style="background-color:#FFFFFF" rowspan="1">intergenic</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">3169966</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" colspan="4" style="background-color:#FFFFFF" rowspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1, 2, 3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A4700</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">propionate—CoA ligase</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">4587482</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Asn</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Thr</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">240</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2, 3</td></tr><tr><td align="center" rowspan="5" style="background-color:#FFFFFF" colspan="1">Bovine / Poultry</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Locus Tag <xref ref-type="table-fn" rid="t002fn001"><sup>a</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Annotation <xref ref-type="table-fn" rid="t002fn002"><sup>b</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Genome Position <xref ref-type="table-fn" rid="t002fn001"><sup>a</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">nt Poultry-associated</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">nt Bovine-associated</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Substitution <xref ref-type="table-fn" rid="t002fn003"><sup>c</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">aa Poultry</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">aa Bovine</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">aa Position</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Method</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A1094</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">protein YdcF</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1055798</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Thr</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ala</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">43</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1, 2, 3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A2591</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">hemolysin-3</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2519134</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Phe</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Val</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">32</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A2812</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">methyltransferase family protein</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2713142</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ser</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Leu</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">39</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1, 2, 3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A4467</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">carbonate dehydratase</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">4347265</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ala</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Asp</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">22</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1, 2, 3</td></tr></tbody></table></alternatives><table-wrap-foot><fn id="t002fn001"><p><sup>a</sup> = Locus/Position in <italic>S</italic>. Kentucky CVM29188</p></fn><fn id="t002fn002"><p><sup>b</sup> = Annotation in <italic>S</italic>. Kentucky CVM29188 (accession no. ABAK02000001.1)</p></fn><fn id="t002fn003"><p><sup>c</sup> = S(synonymous), NS(nonsynonymous)</p></fn><fn id="t002fn004"><p>1 = Parsnp</p></fn><fn id="t002fn005"><p>2 = Lyve-SET</p></fn><fn id="t002fn006"><p>3 = CFSAN SNP Pipeline</p></fn></table-wrap-foot></table-wrap><p>In a comparison of bovine-associated isolates to poultry-associated isolates an average of 217, 247, and 281 SNP differences were identified between the two by the Parsnp, Lyve-SET, and CFSAN SNP Finder analyses, respectively (Parsnp range = 62 to 308 SNPs, Lyve-SET range = 72 to 377 SNPs, and CFSAN SNP Pipeline range = 72 to 489). Within these SNP matrices there were four SNPs in protein coding genes, all resulting in non-synonymous mutations, that defined the bovine-poultry divergence for all strains (SeKA_A1094, SeKA_A2591, SeKA_A2812, SeKA_A4467) (<xref ref-type="table" rid="pone.0161225.t002">Table 2</xref>). No evidence of the role of these genes in host-specificity, colonization of the gut by <italic>S</italic>. <italic>enterica</italic> or persistence of <italic>S</italic>. <italic>enterica</italic> in cows, poultry, poultry and bovine products, or the environment could be found in the literature. However, hemolysin-3 (SeKA_A2591) has been shown in Gram-negative and Gram-positive organisms to be involved in the infection process of other mammals, particularly <italic>Vibrio vulnificus</italic> and <italic>Bacillus cereus</italic> [<xref rid="pone.0161225.ref050" ref-type="bibr">50</xref>][<xref rid="pone.0161225.ref051" ref-type="bibr">51</xref>]. The hemolysin-3 in <italic>S</italic>. Kentucky demonstrates 71% and 45% amino acid identity to that of <italic>V</italic>. <italic>vulnificus</italic> and <italic>B</italic>. <italic>cereus</italic>. <italic>In vitro</italic> and <italic>in vivo</italic> assays need to be conducted to further elucidate the potential roles of these protein-coding genes in persistence in the poultry and bovine hosts or specificity to either environment.</p><p>A Bayesian analysis of SNP characters using the STRUCTURE v 2.3.4 program was consistent with the inferred ML and MP phylogenies (<xref ref-type="fig" rid="pone.0161225.g008">Fig 8</xref>) in showing that there were primarily two distinct groups. However, these results provided additional details that help explain the placement of certain isolates within the phylogenetic analysis. For example, STRUCTURE showed that isolates within Cluster 1.2.3 have SNP profiles that are, for the most part, distinct from all others (blue profiles). In contrast, clusters 1.1, 1.2.1, and 1.2.2 showed evidence of admixture (blue/orange profiles) where those isolates had some proportion of SNPs that are indicative of clusters 1.2.3 (blue profiles) and Lineage 2.0 isolates (orange profiles). Cluster 1.1 isolates had SNP profiles more closely related to clusters 2.1 to 2.4 than to 1.2.3. All poultry isolates all show approximately similar SNP profiles with the basal cow isolates in clusters 2.1 and 2.2 indicating a high level of similarity among the cow and poultry isolates within evolutionary Lineage 2.0.</p><fig id="pone.0161225.g008" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0161225.g008</object-id><label>Fig 8</label><caption><title>STRUCTURE analysis of the proportion of each isolate’s SNP profile attributed to each of the different groups.</title><p>Dashed line = Lineage 1.0/2.0 divergence. Profiles to the left of the solid black line = bovine-associated isolates, while profiles to the right of the solid black line = poultry-associated isolates. Asterisk = human clinical isolate CDC 191.</p></caption><graphic xlink:href="pone.0161225.g008"></graphic></fig></sec><sec id="sec007"><title>Plasmid Detection</title><p>Using the assembled genomes, multiple plasmids were detected in the <italic>S</italic>. Kentucky ST152 isolates while several were identified as plasmid-free (<xref ref-type="table" rid="pone.0161225.t003">Table 3</xref> and <xref ref-type="supplementary-material" rid="pone.0161225.s002">S2 Table</xref>). Thirty three poultry-associated isolates encoded sequences similar to the ColV plasmid pCVM29188_146 plasmid (IncFIB), which is somewhat consistent with previous studies of poultry-isolated <italic>S</italic>. Kentucky in North America that have shown that the majority of these strains (72.9%) harbored markers of ColV plasmids [<xref rid="pone.0161225.ref052" ref-type="bibr">52</xref>]. However, this study also demonstrated that <italic>S</italic>. Kentucky isolates collected on farms were more likely to harbor this plasmid than those collected from retail meats [<xref rid="pone.0161225.ref052" ref-type="bibr">52</xref>], while the majority of isolates analyzed here were recovered from the latter. This plasmid has been shown to be involved in enhanced survival of <italic>S</italic>. Kentucky in poultry and is highly similar in gene content and nucleotide sequence similarity to the ColV plasmid of avian pathogenic <italic>Escherichia coli</italic> (APEC) [<xref rid="pone.0161225.ref052" ref-type="bibr">52</xref>]. The presence of this plasmid was restricted to poultry-associated isolates, providing further evidence that it is involved in interactions with the poultry host. However, it should be noted that Fricke et al. [<xref rid="pone.0161225.ref012" ref-type="bibr">12</xref>] identified six bovine-associated isolates with PCR markers indicative of the presence of APEC-like plasmids similar to those of pCVM29188_146 suggesting that more bovine-specific <italic>S</italic>. Kentucky need to be evaluated for the presence of this or highly similar plasmids. Although, Johnson et al. [<xref rid="pone.0161225.ref052" ref-type="bibr">52</xref>]demonstrated the role of this plasmid in enhanced extracellular survival in poultry, its absence from poultry-associated <italic>S</italic>. Kentucky suggests there are other factors involved in specificity of these strains to the poultry host. Similar to Ladely et al. [<xref rid="pone.0161225.ref013" ref-type="bibr">13</xref>] the IncFIB plasmid type was not evenly distributed across poultry-associated strains as it was identified in 81% of cluster 2.4.1.1 to 2.4.2 isolates but only in 25% of cluster 2.3.1 to 2.3.3.2 isolates.</p><table-wrap id="pone.0161225.t003" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0161225.t003</object-id><label>Table 3</label><caption><title>Plasmids identified by PlasmidFinder in genomes analyzed in this study.</title></caption><alternatives><graphic id="pone.0161225.t003g" xlink:href="pone.0161225.t003"></graphic><table frame="hsides" rules="groups"><colgroup span="1"><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col></colgroup><thead><tr><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">ST</th><th align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"> Poulty/Bovine</th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Plasmid Replicon</th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Number of Plasmids Detected</th></tr></thead><tbody><tr><td align="center" rowspan="11" style="background-color:#FFFFFF" colspan="1">ST152</td><td align="center" rowspan="5" style="background-color:#FFFFFF" colspan="1">Poultry-associated</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">IncFIB</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">33</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">IncX1</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">58</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">IncHI2</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">12</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">Col156</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">IncI1</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">29</td></tr><tr><td align="center" rowspan="6" style="background-color:#FFFFFF" colspan="1">Bovine-associated</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">IncI1</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">34</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">IncI2</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">IncA/C2</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">4</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ColpVC</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">Col8282</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">3</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">No plasmid detected</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">12</td></tr><tr><td align="center" colspan="2" rowspan="2" style="background-color:#FFFFFF">ST198</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">IncI1</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">No plasmid detected</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">11</td></tr></tbody></table></alternatives></table-wrap><p>The most frequently detected plasmid sequences in poultry-associated isolates were those similar to the canonical pCVM29188_46 (IncX1) plasmid, which was also not detected in any of the bovine-associated isolates (<xref ref-type="table" rid="pone.0161225.t003">Table 3</xref> and <xref ref-type="supplementary-material" rid="pone.0161225.s002">S2 Table</xref>). The biological role of this plasmid has not been well-elucidated, but its high prevalence among these strains indicates it may play a significant role in the survival of <italic>S</italic>. Kentucky within the poultry host, poultry production environment, or in transmission of <italic>S</italic>. Kentucky between animals. However, this would need to be further evaluated <italic>in vivo</italic>. Coupling the plasmid presence/absence data with the inferred phylogeny suggests that poultry-associated <italic>S</italic>. Kentucky acquired this plasmid, as well as the IncFIB plasmid after diverging from the most recent common ancestor shared with the bovine-associated isolates.</p><p>Within the bovine-associated <italic>S</italic>. Kentucky 12 isolates were identified as plasmid-free and these isolates belonged to clusters 1.1, 1.2.1, 2.1, and 2.2 (<xref ref-type="table" rid="pone.0161225.t003">Table 3</xref> and <xref ref-type="supplementary-material" rid="pone.0161225.s002">S2 Table</xref>). Isolates from several clusters harbored a variety of plasmids, including those belonging to IncI1 (34 isolates), IncI2 (3 isolates), and IncA/C2 (4 isolates) replicon types, and plasmids identified by PlasmidFinder as Col8282 (3 isolates) and one as ColpVC. The IncI1 plasmid, represented by sequence AOYZ01000068.1 in GenBank of <italic>S</italic>. Kentucky 5349, was the most frequently detected plasmid sequence among the bovine-associated isolates. This sequence is ca. 92 kb, encodes 100 ORFs and is somewhat similar to plasmid sequences available in the NCBI database. These include plasmid pSTY1-1898 of <italic>S</italic>. Typhimurium str. USDA-ARS-USMARC-1898 (coverage = 81%, similarity = 99%) and <italic>S</italic>. Kentucky plasmid pCS0010A_95 (coverage = 79%, similarity = 99%), among several others.</p><p>The majority of isolates that harbored plasmids were from the mid-Atlantic region of the United States. The absence of plasmids in many bovine-associated isolates (25%) suggests they are not necessary for survival or persistence within the bovine gastrointestinal tract or the dairy farm environment. However, the high level prevalence of IncI1 plasmids in cluster 1.2.3 isolates indicates that they may provide an advantage in these environments, and their restriction to isolates from the mid-Atlantic and Northeast United States may be indicative of their importance to the ecology of these strains in this region.</p></sec><sec id="sec008"><title>Phylogeny and Genomic Polymorphisms within the ST198 Isolates (ST complex 56)</title><p>Within the <italic>S</italic>. Kentucky ST198 lineage two major clusters were identified (labeled here as clusters 198.1 and 198.2) (<xref ref-type="fig" rid="pone.0161225.g009">Fig 9</xref>). Cluster 198.1 consists of isolates recovered from agricultural sources in the United States (dairy cow feces, dairy cow milk, ground beef, and ground turkey) and the <italic>S</italic>. Kentucky type strain ATCC 9263. Cluster 198.2 consists of five human clinical isolates, two of these collected from the same patient in Kuwait in 2012 (915c from a sacral wound and 917c from a stool sample) [<xref rid="pone.0161225.ref025" ref-type="bibr">25</xref>], and three were collected from human clinical cases in the United States. It is not known if these three infections were acquired abroad or within the United States, as metadata for these isolates are lacking in the public database. Source attribution would need to be verified before the locations of infections can be confirmed. <italic>S</italic>. Kentucky ST198 infections have been reported in Africa, Eastern Europe, and Southeast Asia and travel-acquired infections with these organisms has been reported in people traveling to these regions [<xref rid="pone.0161225.ref007" ref-type="bibr">7</xref>][<xref rid="pone.0161225.ref008" ref-type="bibr">8</xref>]. Until a more comprehensive geophylogeny of <italic>S</italic>. Kentucky ST198 strains is conducted assumptions about strain traceback are speculative.</p><fig id="pone.0161225.g009" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0161225.g009</object-id><label>Fig 9</label><caption><title>Maximum Parsimony tree of <italic>S</italic>. Kentucky ST198 isolates collected from human clinical cases (cluster 198.2) and dairy cows and food products in North America (198.1).</title><p>The tree was inferred from an alignment of SNPs detected among all <italic>S</italic>. Kentucky ST198 genomes and using the chromosome of <italic>S</italic>. Kentucky CVM29188 as a reference. The tree is based on 42,958 SNPs (Parsnp analysis), of these, 532 SNPs were identified among the ST198 genomes. The tree displayed here is rooted in the distantly related <italic>S</italic>. Kentucky CVM29188. Tree was inferred with 1000 bootstrap replicates. Bar length = number of substitutions.</p></caption><graphic xlink:href="pone.0161225.g009"></graphic></fig><p>Using Parsnp, with <italic>S</italic>. Kentucky CVM29188 as a reference genome, there were 532 SNPs identified in the core-genomes of the ST198 isolates with an average of 209 SNP differences between the clusters 198.1 and 198.2 genomes (range = 169 to 228 SNPs). Cluster 198.1 isolates collected from agricultural sources in the United States had, for the most part, fewer SNPs between each other (mean = 138, range = 10 to 210) than they did with the human clinical isolates (cluster 198.2) (<xref ref-type="table" rid="pone.0161225.t004">Table 4</xref>). Similarly, the human clinical isolates (cluster 198.2) had fewer SNP differences between each other than they did with the cluster 198.1 isolates (mean = 32, range = 2 to 48). Interestingly, in a genome-genome comparison fewer SNPs were detected between ATCC 9263 and 198.2 genomes than between ATCC 9263 and 198.1 genomes. However, there were fewer SNPs between ATCC 9263 and the 198.1 cluster than ATCC 9263 and the 198.2 cluster when the average base pair differences over all of the sequence pairs were calculated per cluster.</p><table-wrap id="pone.0161225.t004" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0161225.t004</object-id><label>Table 4</label><caption><title>SNP differences among <italic>S</italic>. Kentucky ST198 genomes.</title></caption><alternatives><graphic id="pone.0161225.t004g" xlink:href="pone.0161225.t004"></graphic><table frame="hsides" rules="groups"><colgroup span="1"><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col></colgroup><thead><tr><th align="center" colspan="2" style="background-color:#FFFFFF" rowspan="1">Cluster / Genome</th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">ATCC 9263</th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N51290</th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N42453</th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N41913</th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC274</th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC273</th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC938</th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM 43780</th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM 43756</th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM 43824</th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">915c</th></tr></thead><tbody><tr><td align="center" rowspan="7" style="background-color:#FFFFFF" colspan="1">198.1</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ATCC 9263</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N51290</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">195</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N42453</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">201</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">94</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM N41913</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">200</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">193</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">197</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC274</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">210</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">69</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">107</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">206</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC273</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">210</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">71</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">111</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">210</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">10</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ARS-CC938</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">197</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">58</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">98</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">197</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">35</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">33</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td></tr><tr><td align="center" rowspan="5" style="background-color:#FFFFFF" colspan="1">198.2</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM 43780</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">169</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">204</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">210</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">209</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">219</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">219</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">206</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM 43756</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">178</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">211</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">215</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">214</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">224</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">228</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">215</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">19</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">CVM 43824</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">174</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">207</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">211</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">210</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">220</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">224</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">211</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">17</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">22</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">915c</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">177</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">208</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">214</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">213</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">223</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">225</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">212</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">41</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">48</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">44</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1"> </td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">917c</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">179</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">210</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">214</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">213</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">223</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">227</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">214</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">43</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">48</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">44</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2</td></tr></tbody></table></alternatives></table-wrap><p>There were 66 conserved SNP differences identified between clusters 198.1 and 198.2 (<xref ref-type="table" rid="pone.0161225.t005">Table 5</xref>). Of particular interest are the DNA gyrase subunit A (<italic>gyrA</italic>) (AEX15_13770) substitutions Ser83 → Phe in 198.2; Asp87 → Tyr in CVM 43780, CVM 43756, and CVM 43824; and Asp87 → Asn in 915c and 917c. These substitutions confer resistance to nalidixic acid, an attribute that is presumed to have emerged in ST198 isolates in the early 2000s in Egypt[<xref rid="pone.0161225.ref007" ref-type="bibr">7</xref>]. Substitution Ser80 → Ile in DNA topoisomerase IV subunit A (<italic>parC</italic>) (AEX15_04910) conferring resistance to ciprofloxacin, was also observed in 198.2 isolates. These substitutions are characteristic of ST198 strains circulating in North and East Africa and the Middle East [<xref rid="pone.0161225.ref007" ref-type="bibr">7</xref>] suggesting that the infections caused by these strains (<italic>S</italic>. Kentucky CVM 43780, CVM 43756, and CVM 43824) may have been acquired outside of the United States or from exposure to imported products.</p><table-wrap id="pone.0161225.t005" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0161225.t005</object-id><label>Table 5</label><caption><title>SNP differences between 198.1 (non-clinical North American agricultural isolates) and 198.2 (human clinical isolates).</title><p>The reference genome for this analysis is the chromosome of <italic>S</italic>. Kentucky CVM29188.</p></caption><alternatives><graphic id="pone.0161225.t005g" xlink:href="pone.0161225.t005"></graphic><table frame="hsides" rules="groups"><colgroup span="1"><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col></colgroup><thead><tr><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Locus Tag in CVM N51290</th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Locus Tag in CVM29188</th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Annotation <xref ref-type="table-fn" rid="t005fn001"><sup>a</sup></xref></th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Genome Position <xref ref-type="table-fn" rid="t005fn001"><sup>a</sup></xref></th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">nt 198.1</th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">nt 198.2</th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Substitution <xref ref-type="table-fn" rid="t005fn002"><sup>b</sup></xref></th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">aa 198.1</th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">aa 198.2</th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">aa Position</th></tr></thead><tbody><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_00985</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A0118</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">putative cytoplasmic protein</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">112694</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Asn</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ser</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">215</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_01465</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A0272</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ABC transporter ATP-binding protein</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">259861</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">S</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Asn</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Asn</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">352</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_07125</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A0450</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">outer membrane protein F</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">449431</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">S</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ala</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ala</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">310</td></tr><tr><td align="left" colspan="3" style="background-color:#FFFFFF" rowspan="1">intergenic</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">511489</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" colspan="4" style="background-color:#FFFFFF" rowspan="1"> </td></tr><tr><td align="left" colspan="3" style="background-color:#FFFFFF" rowspan="1">intergenic</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">528058</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" colspan="4" style="background-color:#FFFFFF" rowspan="1"> </td></tr><tr><td align="left" colspan="3" style="background-color:#FFFFFF" rowspan="1">intergenic</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">768380</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" colspan="4" style="background-color:#FFFFFF" rowspan="1"> </td></tr><tr><td align="left" colspan="3" style="background-color:#FFFFFF" rowspan="1">intergenic</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">932324</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" colspan="4" style="background-color:#FFFFFF" rowspan="1"> </td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_09820</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A1028</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">Nitrate reductase alpha subunit</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">986092</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ser</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Tyr</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">249</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_09905</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A1046</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">virulence factor SrfB</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1006926</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">S</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Asn</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Asn</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">389</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_10005</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A1065</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">PTS system, IIc component</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1028557</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Val</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Gly</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">232</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_10580</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A1172</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">phosphatidylglycerophosphatase B</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1132142</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Thr</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ala</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">184</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_10645</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A1188</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">anthranilate synthase component I</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1148000</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pro</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ser</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">225</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_11230</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A1320</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">hypothetical protein</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1268383</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Gly</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ala</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">47</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_11825</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A1435</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">aspartyl-tRNA synthetase</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1376156</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ala</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Thr</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">273</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_11880</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A1448</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">flagellar protein FlhE</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1388508</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">S</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Leu</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Leu</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">624</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_11945</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A1461</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">transcriptional activator FlhD</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1402136</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">S</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ser</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ser</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">4</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_12480</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A1563</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">arsenical pump-driving ATPase</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1479380</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Leu</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ser</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">578</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_13315</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A1750</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">beta-D-glucoside glucohydrolase</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1662011</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">S</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Thr</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Thr</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">560</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_13615</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A1814</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">nucleoid-associated protein NdpA</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1730145</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Asn</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Asp</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">136</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_13770</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A1846</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">DNA gyrase subunit A (<italic>gyrA</italic>)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1764089</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ser</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Phe</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">83</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_13940</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A1884</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">O-succinylbenzoate synthase</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1805065</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Glu</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Asp</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">44</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_15785</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A2190</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">DNA repair protein RecO</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2129001</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Lys</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Glu</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">156</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_21540</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A2229</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">phospho-2-dehydro-3-deoxyheptonate aldolase</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2169354</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">S</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Asn</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Asn</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">149</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_21995</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A2343</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">murein hydrolase effector LrgB</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2282024</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">S</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Gly</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Gly</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">38</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_22185</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A2383</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">putative type III secretion system effector protein OrgC</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2317497</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ser</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Leu</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">98</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_22530</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A2451</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">sulfate adenylyltransferase subunit 1</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2379190</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ile</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ser</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">339</td></tr><tr><td align="left" colspan="3" style="background-color:#FFFFFF" rowspan="1">intergenic</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2476378</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" colspan="4" style="background-color:#FFFFFF" rowspan="1"> </td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_04160</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A2564</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">nickel/cobalt efflux protein RcnA</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2496525</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pro</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ser</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">31</td></tr><tr><td align="left" colspan="3" style="background-color:#FFFFFF" rowspan="1">intergenic</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2497451</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" colspan="4" style="background-color:#FFFFFF" rowspan="1"> </td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_04380</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A2619</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">D-erythrose-4-phosphate dehydrogenase</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2542815</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pro</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ser</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">224</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_04910</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A2749</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">DNA topoisomerase IV subunit A (<italic>parC</italic>)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2654521</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ser</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ile</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">80</td></tr><tr><td align="left" colspan="3" style="background-color:#FFFFFF" rowspan="1">intergenic</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2760667</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" colspan="4" style="background-color:#FFFFFF" rowspan="1"> </td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_03530</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A3124</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">cytoplasmic protein</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2998220</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">S</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Thr</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Thr</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">395</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_02625</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A3321</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">putative glucarate transporter</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">3208206</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Leu</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Gln</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">355</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_02555</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A3336</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">ADP-heptose—LPS heptosyltransferase</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">3221877</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">S</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pro</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pro</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">122</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_02175</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A3395</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">PTS sorbose transporter subunit IIC</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">3280995</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Gly</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Asp</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">241</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_14010</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A3451</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">trimethylamine N-oxide reductase I catalytic subunit</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">3334071</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">S</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Cys</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Cys</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">320</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_14230</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A3482</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">phosphate transporter permease subunit PstC</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">3369116</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pro</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">His</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">74</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_14255</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A3487</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">glucosamine—fructose-6-phosphate aminotransferase</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">3375283</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ile</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Val</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">490</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_20905</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A3529</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">threonine dehydratase</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">3421216</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">S</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ala</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ala</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">446</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_21030</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A3553</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">putative common antigen polymerase</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">3445490</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">S</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Leu</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Leu</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">125</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_21080</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A3565</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">porphobilinogen deaminase</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">3453638</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Asp</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Glu</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">117</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_21220</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A3595</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">chondroitin sulfate/heparin utilization regulation protein</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">3480727</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">S</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Asn</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Asn</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">103</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_21260</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A3603</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">sec-independent translocase</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">3489186</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pro</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ser</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">142</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_21305</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A3612</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">proline dipeptidase</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">3500226</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">S</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ser</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ser</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">179</td></tr><tr><td align="left" colspan="3" style="background-color:#FFFFFF" rowspan="1">intergenic</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">3575800</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" colspan="4" style="background-color:#FFFFFF" rowspan="1"> </td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_21420</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A3805</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">thiamine-phosphate pyrophosphorylase</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">3686081</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ala</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ser</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">57</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_18585</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A3887</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">LexA repressor</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">3764969</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">S</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ser</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ser</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">60</td></tr><tr><td align="left" colspan="3" style="background-color:#FFFFFF" rowspan="1">intergenic</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">3770533</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" colspan="4" style="background-color:#FFFFFF" rowspan="1"> </td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_18425</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A3921</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">Na+/H+ antiporter</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">3813232</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">S</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ser</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ser</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">30</td></tr><tr><td align="left" colspan="3" style="background-color:#FFFFFF" rowspan="1">intergenic</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">3829881</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" colspan="4" style="background-color:#FFFFFF" rowspan="1"> </td></tr><tr><td align="left" colspan="3" style="background-color:#FFFFFF" rowspan="1">intergenic</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">3832063</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" colspan="4" style="background-color:#FFFFFF" rowspan="1"> </td></tr><tr><td align="left" colspan="3" style="background-color:#FFFFFF" rowspan="1">intergenic</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">3896075</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" colspan="4" style="background-color:#FFFFFF" rowspan="1"> </td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_17850</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A4040</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">putative cytoplasmic protein</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">3929069</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">S</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Thr</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Thr</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">54</td></tr><tr><td align="left" colspan="3" style="background-color:#FFFFFF" rowspan="1">intergenic</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">4002925</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" colspan="4" style="background-color:#FFFFFF" rowspan="1"> </td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_20245</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A4293</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">chitinase</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">4165915</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">S</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Val</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Val</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">533</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_20105</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A4321</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">isoleucyl-tRNA synthetase</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">4192511</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">S</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Thr</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Thr</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">62</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_17125</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A4346</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">carnitine operon protein CaiE</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">4219742</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Glu</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Lys</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">128</td></tr><tr><td align="left" colspan="3" style="background-color:#FFFFFF" rowspan="1">intergenic</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">4233585</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" colspan="4" style="background-color:#FFFFFF" rowspan="1"> </td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_16055</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A4409</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">4286504</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ser</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Pro</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">265</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_16220</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A4445</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">hypothetical protein</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">4325107</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">S</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Gly</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Gly</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">137</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_16390</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A4481</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">putative fimbrial outer membrane usher protein</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">4359640</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Leu</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Met</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">704</td></tr><tr><td align="left" colspan="3" style="background-color:#FFFFFF" rowspan="1">intergenic</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">4463447</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">T</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" colspan="4" style="background-color:#FFFFFF" rowspan="1"> </td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_22970</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A4665</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">fimbrial outer membrane usher protein StbC</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">4548312</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">C</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Val</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Gly</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">119</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_06235</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A4762</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">2-aminoethylphosphonate transport system permease PhnU</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">4645904</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">NS</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ile</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Thr</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">92</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_06585</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A4840</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">copper exporting ATPase</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">4725203</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">A</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">G</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">S</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ser</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Ser</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">111</td></tr></tbody></table></alternatives><table-wrap-foot><fn id="t005fn001"><p><sup>a</sup> = Position/annotation in <italic>S</italic>. Kentucky CVM29188 (accession no. ABAK02000001.1)</p></fn><fn id="t005fn002"><p><sup>b</sup> = S(synonymous), NS(nonsynonymous)</p></fn></table-wrap-foot></table-wrap><p>A Bayesian analysis of SNP differences among isolates using the STRUCTURE program indicated that for the most part 198.1 and 198.2 isolates were distinct with the exception of ATCC 9263 and CVM N41913, which were basal within the cluster 198.1 lineage (<xref ref-type="fig" rid="pone.0161225.g010">Fig 10</xref>). For these two isolates a proportion of SNPs detected in their genomes (ATCC 9263 = 0.24 and CVM N41913 = 0.078) were characteristic of those identified in 198.2.</p><fig id="pone.0161225.g010" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0161225.g010</object-id><label>Fig 10</label><caption><title>STRUCTURE analysis of the proportion of each isolate’s SNP profile attributed to each of the different clusters (198.1 and 198.2).</title></caption><graphic xlink:href="pone.0161225.g010"></graphic></fig><p>All cluster 198.2 genomes were identified as encoding ORFs homologous to the complete sequence of <italic>Salmonella</italic> Genomic Island 1 inserted at the <italic>trmE</italic>-<italic>yidY</italic> locus (SGI-K used as a reference; NCBI accession AY463797) (<xref ref-type="fig" rid="pone.0161225.g011">Fig 11</xref>). However, the structure of this island was difficult to discern due to the presence of SGI1 ORFs on multiple contigs in the 198.2 cluster genomes. SGI1 is an integrative and mobilizable element found in several <italic>S</italic>. <italic>enterica</italic> serovars and other non-salmonellae and responsible for resistance to multiple antibiotics [<xref rid="pone.0161225.ref053" ref-type="bibr">53</xref>][<xref rid="pone.0161225.ref054" ref-type="bibr">54</xref>]. The structure of this island is known to be highly variable [<xref rid="pone.0161225.ref044" ref-type="bibr">44</xref>][<xref rid="pone.0161225.ref055" ref-type="bibr">55</xref>]. Four of five SGI1 encoding genomes encoded regions known for resistance to mercury. However, this regions is absent from in CVM 43756 (<xref ref-type="fig" rid="pone.0161225.g011">Fig 11</xref>). For all cluster 198.2 genomes excluding 915c, <italic>strB</italic> (streptomycin phosphotransferase) and <italic>strA</italic> were not detected.</p><fig id="pone.0161225.g011" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0161225.g011</object-id><label>Fig 11</label><caption><title>BLASTN comparison of SGI1-K against the ST198 genomes (Top).</title><p>Blue squares indicate level of similarity, red squares indicate that homologs were not detected in the query genome sequence, orange squares = homolog was identified but at < 90% coverage. BLASTN comparison of a ca. 17 kb region inserted at the <italic>trmE</italic>-<italic>yidY</italic> locus in cluster 198.1 genomes (Bottom).</p></caption><graphic xlink:href="pone.0161225.g011"></graphic></fig><p>At the <italic>trmE</italic>-<italic>yidY</italic> insertion locus (AEX15_14100, AEX15_14180) all 198.1 genomes, excluding ATCC 9263, encoded a ca. 17 kb region with ORFs annotated as an integrase, transcriptional regulator, helicase, five ORFs annotated as hypothetical proteins, a single ORF annotated as 2-hydroxyacid dehydrogenase, ATP F0F1 synthase synthase, addiction module antitoxin, resolvase, and three restriction endonuclease ORFs. To-date, this region has only been identified in these six <italic>S</italic>. Kentucky ST198 genomes, <italic>S</italic>. Seftenberg ATCC 43845 and <italic>S</italic>. Agona strains 18.H.07, 557928, and 620239.</p><p>Based on the PlasmidFinder analysis, plasmids were not detected in any of the ST198 strains except for <italic>S</italic>. Kentucky CVM 51290 (<xref ref-type="table" rid="pone.0161225.t003">Table 3</xref>). However, strains harboring plasmids carrying antibiotic resistance genes have been collected from human clinical cases [<xref rid="pone.0161225.ref009" ref-type="bibr">9</xref>] suggesting that, like ST152 and other <italic>S</italic>. <italic>enterica</italic> serovars, plasmid presence is variable in ST198. The IncI1 plasmid sequence identified in <italic>S</italic>. Kentucky CVM 51290 is similar to others currently in a NCBI plasmid database and shows 99% sequence similarity across 90% of the putative plasmid region with plasmid pSL476_91 from S. Heidelberg str. SL476, and 99% similarity across 88% of the plasmid region with <italic>S</italic>. Kentucky plasmid pCS0010A_95.</p><p>Few conclusions, based on genomic data, can be made about the ST198 group at this time due to the limited number of available <italic>S</italic>. Kentucky ST198 genome sequences deposited in public databases. However, it is clear that there is considerable genomic diversity among the genomes that have been sequenced to date. Sequencing of more ST198 genomes will allow for a more comprehensive understanding of the geophylogeny, global diversity, and presence of variable regions, such as SGI1, in these isolates.</p><p>It is important to note that ST198 has been isolated from avian and bovine sources in the United States [<xref rid="pone.0161225.ref006" ref-type="bibr">6</xref>] indicating that it may have the potential to become established in poultry flocks, dairy herds, other livestock, and/or wildlife in the United States. However, more work needs to be conducted to understand its apparent limited distribution in these animals as well as the presence of any potential wildlife reservoirs. Further, more work needs to be conducted to estimate the virulence potential of the ST198 that is endemic in the United States and abroad.</p></sec><sec id="sec009"><title>Differences between <italic>S</italic>. Kentucky ST152 and ST198 Isolates</title><p>Although frequently considered to be similar groups of strains due to their serological-based nomenclature, <italic>S</italic>. Kentucky ST198 and <italic>S</italic>. Kentucky ST152 isolates demonstrate considerable differences in their genetic backgrounds that have not been adequately described. Using assembled genomes, with the chromosome of <italic>S</italic>. Kentucky CVM29188 as a reference, there were between 27,369 and 30,485 SNP differences between <italic>S</italic>. Kentucky CVM29188 and representatives of other subclade A1 serovars in a core-genome alignment of length 3,465,932 bp (ca. 72% coverage of the <italic>S</italic>. Kentucky CVM29188 chromosome) (<xref ref-type="table" rid="pone.0161225.t006">Table 6</xref>, <xref ref-type="fig" rid="pone.0161225.g012">Fig 12</xref>). In this analysis there were between 29,952 and 29,990 SNPs between CVM29188 and ST198 genomes. Thirteen serovars had fewer SNP differences with <italic>S</italic>. Kentucky CVM29188 than did <italic>S</italic>. Kentucky ST198 with CVM29188 (<xref ref-type="table" rid="pone.0161225.t006">Table 6</xref>, <xref ref-type="fig" rid="pone.0161225.g012">Fig 12</xref>), while, 11 serovars of subclade A1 demonstrated fewer SNP differences with ST198 <italic>S</italic>. Kentucky ATCC 9263 than did <italic>S</italic>. Kentucky CVM29188 with <italic>S</italic>. Kentucky ATCC 9263. These data indicate a significant difference in the nucleotide sequence content between ST152 and ST198 genomes and coupled with the core-genome phylogeny (<xref ref-type="fig" rid="pone.0161225.g001">Fig 1</xref>) indicate that <italic>S</italic>. Kentucky ST152 and ST198 are more similar to other serovars than they are to each other.</p><fig id="pone.0161225.g012" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0161225.g012</object-id><label>Fig 12</label><caption><title>Number of SNP differences between <italic>S</italic>. Kentucky CVM29188 (ST152) and other <italic>S</italic>. <italic>enterica</italic> subclade A1 genomes (Top).</title><p>Number of SNP differences between <italic>S</italic>. Kentucky ATCC 9263 (ST198) and other <italic>S</italic>. <italic>enterica</italic> subclade A1 genomes (bottom). X-axes show genomes used in this analysis and Y-axes show number of SNP differences.</p></caption><graphic xlink:href="pone.0161225.g012"></graphic></fig><table-wrap id="pone.0161225.t006" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0161225.t006</object-id><label>Table 6</label><caption><title>Number of SNP differences between ST198/ST152 and subclade A1 serovars.</title><p>For this analysis representatives of subclade A1 were selected. Parsnp was used to identify core-genome SNPs among the sequenced isolates. The chromosome of <italic>S</italic>. Kentucky CVM29188 was used as a reference genome for the SNP analysis.</p></caption><alternatives><graphic id="pone.0161225.t006g" xlink:href="pone.0161225.t006"></graphic><table frame="hsides" rules="groups"><colgroup span="1"><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col></colgroup><thead><tr><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Serovar/Strain</th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">No. SNP differences with <italic>S</italic>. Kentucky CVM29188</th><th align="center" style="background-color:#000000" rowspan="1" colspan="1"> </th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Serovar/Strain</th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">No. SNP differences with <italic>S</italic>. Kentucky ATCC 9263</th></tr></thead><tbody><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Cubana CFSAN001083</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">27369</td><td align="left" style="background-color:#000000" rowspan="1" colspan="1"> </td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Senftenberg ATCC 43845</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">27140</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Tennessee TXSC-TXSC08-21</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">27457</td><td align="left" style="background-color:#000000" rowspan="1" colspan="1"> </td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Agona 632182–2</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">27743</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Meleagridis 0047</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">27821</td><td align="left" style="background-color:#000000" rowspan="1" colspan="1"> </td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Worthington ATCC 9607</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">27746</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Soerenga 695</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">27927</td><td align="left" style="background-color:#000000" rowspan="1" colspan="1"> </td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Havana CFSAN001082</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">28073</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Rissen 150</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">27958</td><td align="left" style="background-color:#000000" rowspan="1" colspan="1"> </td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Paratyphi B ATCC BAA-1585</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">28638</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Seftenberg 604314</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">28066</td><td align="left" style="background-color:#000000" rowspan="1" colspan="1"> </td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Albany ATCC 51960</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">28658</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Paratyphi B ATCC BAA-1585</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">28156</td><td align="left" style="background-color:#000000" rowspan="1" colspan="1"> </td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Seftenberg 604314</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">29329</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Agona ATCC 51957</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">28445</td><td align="left" style="background-color:#000000" rowspan="1" colspan="1"> </td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Rissen 150</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">29582</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Albany ATCC 51960</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">28815</td><td align="left" style="background-color:#000000" rowspan="1" colspan="1"> </td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Meleagridis 0047</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">29614</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Derby 626</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">29621</td><td align="left" style="background-color:#000000" rowspan="1" colspan="1"> </td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Cubana CFSAN001083</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">29685</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Havana CFSAN001082</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">29635</td><td align="left" style="background-color:#000000" rowspan="1" colspan="1"> </td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Tennessee TXSC-TXSC08-21</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">29768</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Senftenberg ATCC 43845</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">29718</td><td align="left" style="background-color:#000000" rowspan="1" colspan="1"> </td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Kentucky CVM29188 (ST152)</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">29971</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Agona 632182–2</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">29940</td><td align="left" style="background-color:#000000" rowspan="1" colspan="1"> </td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Agona ATCC 51957</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">29974</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Kentucky ATCC 9263 (ST198)</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">29971</td><td align="left" style="background-color:#000000" rowspan="1" colspan="1"> </td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Soerenga 695</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">30015</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Weltevreden HI-N05-537</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">30078</td><td align="left" style="background-color:#000000" rowspan="1" colspan="1"> </td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Derby 626</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">30451</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Worthington ATCC 9607</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">30140</td><td align="left" style="background-color:#000000" rowspan="1" colspan="1"> </td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Weltevreden HI-N05-537</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">31088</td></tr><tr><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Sloterdijk ATCC 15791</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">30485</td><td align="left" style="background-color:#000000" rowspan="1" colspan="1"> </td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1"><italic>S</italic>. Sloterdijk ATCC 15791</td><td align="right" style="background-color:#FFFFFF" rowspan="1" colspan="1">31309</td></tr></tbody></table></alternatives></table-wrap><p>In an analysis comparing only ST152 genomes to ST198 genomes 37,769 SNP differences were detected (<xref ref-type="supplementary-material" rid="pone.0161225.s003">S3 Table</xref>). These SNPs were annotated and results demonstrated a significant number of polymorphisms in the protein-coding regions of the genome (<xref ref-type="supplementary-material" rid="pone.0161225.s004">S4 Table</xref>). Of a total of 4452 annotated protein-coding genes, at least one SNP was detected in 3713 (83.5%) genes while no SNPs were detected in 737 (16.5%) genes. Three hundred seventeen of these genes had ≥ 20 conserved SNP differences with ST152. Of particular interest are several protein coding genes that are involved with interactions with the animal host environment, specifically in the colonization and infection processes by <italic>S</italic>. <italic>enterica</italic>. For example, 239 conserved SNP differences were identified in SeKA_A3913, a 16,683 bp ORF annotated as a conserved hypothetical protein, located within SPI-4, and involved in colonization of the bovine gastrointestinal tract [<xref rid="pone.0161225.ref056" ref-type="bibr">56</xref>]. It should be noted that there was an appreciable level of sequence divergence in this gene within ST152 isolates, particularly in isolate ABB1087-1, and this is most likely due to the likelihood of synonymous substitutions to accumulate across a large protein coding gene. SeKA_A0255 of ST152, an ORF homologous to the secreted virulence protein SlrP (STM0800) of <italic>S</italic>. Typhimurium LT2, had 84 conserved SNP differences with ST198 genomes. A large repetitive protein (SeKA_A2250) homologous to BapA (STM2689) involved in host-colonization, organ invasion, and biofilm formation [<xref rid="pone.0161225.ref057" ref-type="bibr">57</xref>] encoded 68 conserved SNP differences between the two STs. An invasion-like protein (SeKA_A1129), homologous to STM1669 (ZirT), involved in virulence modulation [<xref rid="pone.0161225.ref058" ref-type="bibr">58</xref>] encoded 51 conserved SNP differences between the two STs. ShdA (SeKA_A1896), an outer-membrane protein that is expressed in the intestine and is involved in long-term fecal shedding [<xref rid="pone.0161225.ref059" ref-type="bibr">59</xref>][<xref rid="pone.0161225.ref060" ref-type="bibr">60</xref>], had 48 conserved SNP differences between the two STs. Multiple effector proteins also demonstrated appreciable divergence between ST152 and ST198 (SeKA_A1084 = <italic>sseJ</italic>; SeKA_A1053 = <italic>sifB</italic>; SeKA_A1621 = <italic>sopA</italic>; SeKA_A0837 = <italic>sseC</italic>, SeKA_A0835 = <italic>sseB</italic>; SeKA_A2465 = <italic>sopD</italic>; SeKA_A2283 = <italic>pipB</italic>; SeKA_A2398 = <italic>sipD</italic>; SeKA_A0839 = <italic>sseE</italic>; SeKA_A2393 = <italic>sptP</italic>; SeKA_A0841 = <italic>sseF</italic>; SeKA_A2380 = <italic>avrA</italic>). These proteins interact with host-cells and are intimately involved in the infection process [<xref rid="pone.0161225.ref061" ref-type="bibr">61</xref>][<xref rid="pone.0161225.ref062" ref-type="bibr">62</xref>][<xref rid="pone.0161225.ref063" ref-type="bibr">63</xref>] and their sequence divergence may result in different interactions in the human, cow, and poultry hosts, with the potential for different outcomes for each host. However, to fully assess the biological consequences of these differences <italic>in vivo</italic> analyses targeting these regions would need to be conducted.</p><p>Regions identified in one ST but absent in the other, as well as regions present in both but flanked by unique ORFs were identified by conducting a whole-genome reciprocal BLASTP analysis (<xref ref-type="table" rid="pone.0161225.t007">Table 7</xref>). Sixteen regions of contiguous protein-coding genes present in ST198 genomes but absent in ST152 genomes, or highly diverged and flanked by unique regions were identified. Of these, six were completely or partially identified as genomic islands. Of particular interest in the ST198 genomes is an eight ORF region (region 3 in <xref ref-type="table" rid="pone.0161225.t007">Table 7</xref>) responsible for sialic acid transport, a key feature of mammalian pathogens that allows them to scavenge sialic acid and utilize it as a carbon source in the host intestine (AEX15_07505, AEX15_07550) [<xref rid="pone.0161225.ref064" ref-type="bibr">64</xref>][<xref rid="pone.0161225.ref065" ref-type="bibr">65</xref>][<xref rid="pone.0161225.ref066" ref-type="bibr">66</xref>]. This region is flanked on one side by a tRNA-Ser locus suggesting it is a putative transmissible genomic island. A BLASTP analysis demonstrates there is a unidirectional match at low to moderate amino acid similarity (23 to 70% similarity) when these ORFs are compared to ST152 genomes indicating they are highly diverged from ORFs with a similar functional annotations encoded in the ST152 backbone. This sialic acid transport region was identified in other <italic>S</italic>. <italic>enterica</italic> serotypes, many of which are known pathogens of humans and other mammals, such as <italic>S</italic>. Typhimurium, <italic>S</italic>. Paratyphi A, <italic>S</italic>. Enteritidis, and <italic>S</italic>. Dublin. Another region of interest within the ST198 genomes consists of 15 ORFs involved inositol catabolism (region 4) (AEX15_17525, AEX15_17625). This region was also identified in several other serovars and has been identified as a myo-Inositol utilization island by Kröger and Fuchs [<xref rid="pone.0161225.ref067" ref-type="bibr">67</xref>]. Chaudhuri <italic>et al</italic>. [<xref rid="pone.0161225.ref068" ref-type="bibr">68</xref>] demonstrated this region as being associated with proliferation in the host gut. Further, <italic>in vivo</italic> studies have shown that salmonellae with an inactive <italic>reiD</italic> (the orphan regulator of the myo-inositol utilization island) demonstrated reduced fitness following oral infections or chickens, calf, and pigs [<xref rid="pone.0161225.ref068" ref-type="bibr">68</xref>]. <italic>S</italic>. Kentucky ST152 isolates, however, lack this island and, based on their prevalence in this environment, are suitably adapted for persistence in the bovine gut. A third region of interest is a nine ORF region identified within SPI-6 and encoding genes involved in alpha-fimbriae expression, a transcriptional regulator and a mobile genetic element (region 5) (AEX15_23130, AEX15_23170). This region is homologous to the Typhi-colonization factor (tcf) operon (STY0345-STY0348) and should be further investigated as a possible mechanism by which ST198 isolates colonize and infect human and animal hosts.</p><table-wrap id="pone.0161225.t007" orientation="portrait" position="float"><object-id pub-id-type="doi">10.1371/journal.pone.0161225.t007</object-id><label>Table 7</label><caption><title>Regions identified in one <italic>S</italic>. Kentucky ST (ST152 or ST198) and absent or partially present in the other.</title></caption><alternatives><graphic id="pone.0161225.t007g" xlink:href="pone.0161225.t007"></graphic><table frame="hsides" rules="groups"><colgroup span="1"><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col><col align="left" valign="middle" span="1"></col></colgroup><thead><tr><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">ST</th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Region</th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Features</th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Size <xref ref-type="table-fn" rid="t007fn001"><sup>A</sup></xref></th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Flanking Loci in ST198 (CVM N51290 or ATCC 9263) <xref ref-type="table-fn" rid="t007fn002"><sup>B</sup></xref></th><th align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Homologous Flanking Loci in ST152 (CVM29188)</th></tr></thead><tbody><tr><td align="center" rowspan="16" style="background-color:#FFFFFF" colspan="1">198</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">histone acetyltransferase HPA2, hypothetical proteins</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">4405</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_02835, AEX15_02865</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A3272, SeKA_A3273</td></tr><tr><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">putative superfamily I DNA helicases, putative restriction endonuclease, putative type II restriction enzyme methylase subunit, putative cytoplasmic protein, putative inner membrane protein</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">24503</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SEEK9263_16827, SEEK9263_t17079 (tRNA-Leu)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A4142 (tRNA-Leu), SeKA_A4161</td></tr><tr><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">3</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">sialic acid metabolism (transport) <xref ref-type="table-fn" rid="t007fn004"><sup>D</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">9036</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_07510, AEX15_07550 (tRNA-Ser)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A0529, SeKA_A0537 (tRNA-Ser)</td></tr><tr><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">4</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">inositol catabolism</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">23081</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_17525, AEX15_17625</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A4089, SeKA_A4088</td></tr><tr><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">5</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">SPI-6 [Typhi colonization factor (<italic>tcf</italic>) operon] <xref ref-type="table-fn" rid="t007fn004"><sup>D</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">8399</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_23130, AEX15_23170</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A4630, SeKA_A4631</td></tr><tr><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">6</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">anaerobic dimethyl sulfoxide reductase chain A, B, C <xref ref-type="table-fn" rid="t007fn005"><sup>E</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">13280</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_10355, AEX15_10415 <xref ref-type="table-fn" rid="t007fn003"><sup>C</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A1139, SeKA_A1140 <xref ref-type="table-fn" rid="t007fn003"><sup>C</sup></xref></td></tr><tr><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">7</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">DNA-binding protein, phosphorylase, nucleotidyltransferas, exonuclease <xref ref-type="table-fn" rid="t007fn004"><sup>D</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">9323</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_12395, AEX15_12455 (tRNA-Asn)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A1557, SeKA_A1558 (tRNA-Asn)</td></tr><tr><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">8</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">hypothetical protein (secretin domain/putative phage-related secreted protein)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2459</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_09660, AEX15_09685 <xref ref-type="table-fn" rid="t007fn003"><sup>C</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A0993, SeKA_A1001 <xref ref-type="table-fn" rid="t007fn003"><sup>C</sup></xref></td></tr><tr><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">9</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">carbon Starvation, D-gluconate and ketogluconates metabolism, Lactate utilization</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">5674</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_10030, AEX15_10060</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A1070, SeKA_A1073</td></tr><tr><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">10</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">SPI-13 with inserted iron reductase, FMN-dependent NADH-azoreductase, hypothetical proteins</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">3314</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_04620 (tRNA-Phe), AEX15_04645</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A2676 (tRNA-Phe), SeKA_A2689</td></tr><tr><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">11</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">hypothetical proteins, reverse transcriptase (adjacent to SPI-9) <xref ref-type="table-fn" rid="t007fn004"><sup>D</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">4247</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_21645/AEX15_21650, AEX15_21675 <xref ref-type="table-fn" rid="t007fn003"><sup>C</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A2255 (tmRNA), SeKA_A2274 <xref ref-type="table-fn" rid="t007fn003"><sup>C</sup></xref></td></tr><tr><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">12</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">glycine biosynthesis, glycine and serine utilization, serine-glyoxylate cycle, serine biosynthesis, glutamine, glutamate, aspartate and asparagine biosynthesis, threonine and homoserine biosynthesis</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">3958</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_19945, AEX15_19970</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A3752, SeKA_A3753</td></tr><tr><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">13</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">probable integrase remnant</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1050</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_01875, AEX15_01895 (tRNA-Ser)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A0372, SeKA_A0399 (tRNA-Ser)</td></tr><tr><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">14</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">type I restriction-modification system, putative inner membrane protein, hypothetical protein</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">10325</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_20690, AEX15_20730 <xref ref-type="table-fn" rid="t007fn003"><sup>C</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A4188, SeKA_A4198 <xref ref-type="table-fn" rid="t007fn003"><sup>C</sup></xref></td></tr><tr><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">hypothetical protein</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">693</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_14050, AEX15_14060</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A3461, SeKA_A3465</td></tr><tr><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">16</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">hypothetical proteins, phage tail protein, oxidoreductase, transglycosylase <xref ref-type="table-fn" rid="t007fn005"><sup>E</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">25556</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_11375, AEX15_11530</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A1348, SeKA_A1377 <xref ref-type="table-fn" rid="t007fn003"><sup>C</sup></xref></td></tr><tr><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">ST</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Region</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Features</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Size <sup>A</sup></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Flanking Loci in ST152 (CVM29188)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">Homologous Flanking Loci in ST198 (CVM N51290 or ATCC 9263)</td></tr><tr><td align="center" rowspan="17" style="background-color:#FFFFFF" colspan="1">152</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">transcriptional regulator (LysR family) hypothetical protein, major facilitator superfamily MFS_1, 3-oxoacyl-[acyl-carrier-protein] reductase, transketolase, aldehyde-alcohol dehydrogenase 2</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">8040</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A0275, SeKA_A0283 <xref ref-type="table-fn" rid="t007fn003"><sup>C</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_01480, AEX15_01485</td></tr><tr><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">2</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">Entner-Doudoroff pathway, glycolysis and gluconeogenesis, respiratory dehydrogenases 1, inositol catabolism, benzoate degradation <xref ref-type="table-fn" rid="t007fn004"><sup>D</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">23239</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A0372, SeKA_A0399 (tRNA-Ser)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_01875, AEX15_01895 (tRNA-Ser)</td></tr><tr><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">3</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">hypothetical proteins <xref ref-type="table-fn" rid="t007fn004"><sup>D</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">3951</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A0529, SeKA_A0537 (tRNA-Ser)</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_07510, AEX15_07550 (tRNA-Ser)</td></tr><tr><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">4</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">hypothetical proteins, protein TolA</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">4683</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A0993, SeKA_A1001 <xref ref-type="table-fn" rid="t007fn003"><sup>C</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_09685, AEX15_09660 <xref ref-type="table-fn" rid="t007fn003"><sup>C</sup></xref></td></tr><tr><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">5</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">phage ORFs <xref ref-type="table-fn" rid="t007fn004"><sup>D</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">33283</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A1348, SeKA_A1377 <xref ref-type="table-fn" rid="t007fn003"><sup>C</sup></xref><sup>,</sup><xref ref-type="table-fn" rid="t007fn006"><sup>F</sup></xref><sup>,</sup><xref ref-type="table-fn" rid="t007fn007"><sup>G</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_11530, AEX15_11375 <xref ref-type="table-fn" rid="t007fn003"><sup>C</sup></xref></td></tr><tr><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">6</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">hypothetical proteins, protein YibA, protein RhsD, IS1-family insertion element <xref ref-type="table-fn" rid="t007fn004"><sup>D</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">4847</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A1701, SeKA_A1712</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_13120, AEX15_13125</td></tr><tr><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">7</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">hypothetical proteins, putative membrane protein, putative cytoplasmic proteins, transposases, HTH domain protein, integrase <xref ref-type="table-fn" rid="t007fn004"><sup>D</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">11111</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A2096, SeKA_A2114 <xref ref-type="table-fn" rid="t007fn003"><sup>C</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_15380, AEX15_15440 <xref ref-type="table-fn" rid="t007fn003"><sup>C</sup></xref></td></tr><tr><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">8</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">prophage integrase, hypothetical proteins, nuclease-related domain family protein, protein YpjI, DNA repair protein, toxin-antitoxin system (adjacent to SPI-9) <xref ref-type="table-fn" rid="t007fn004"><sup>D</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">12402</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A2255 (tmRNA), SeKA_A2274 <xref ref-type="table-fn" rid="t007fn003"><sup>C</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_21645/AEX15_21650, AEX15_21675 <xref ref-type="table-fn" rid="t007fn003"><sup>C</sup></xref></td></tr><tr><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">9</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">glycolysis and gluconeogenesis, mannitol Utilization</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">5553</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A2624, SeKA_A2631</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_04405, AEX15_04410</td></tr><tr><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">10</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">hypothetical protein, colicin-E7 immunity protein, bacteriophage integrase (fragments overlap with SPIs-8 and 13) <xref ref-type="table-fn" rid="t007fn004"><sup>D</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">4943</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A2676 (tRNA-Phe), SeKA_A2689</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_04620 (tRNA-Phe), AEX15_04645</td></tr><tr><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">11</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">hypothetical proteins, putative autotransporter</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">5146</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A3310, SeKA_A3314</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_02655, AEX15_02660</td></tr><tr><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">12</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">hypothetical proteins</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">5073</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A3672, SeKA_A3678</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_19610, AEX15_19615</td></tr><tr><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">13</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">DNA phosphorothioation, hypothetical proteins, integrase <xref ref-type="table-fn" rid="t007fn004"><sup>D</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">17034</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A4142 (tRNA-Leu), SeKA_A4161 <xref ref-type="table-fn" rid="t007fn003"><sup>C</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SEEK9263_t17079 (tRNA-Leu), SEEK9263_16827 <xref ref-type="table-fn" rid="t007fn003"><sup>C</sup></xref></td></tr><tr><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">14</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">type I restriction-modification system, putative inner membrane protein, hypothetical protein</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">10240</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A4188, SeKA_A4198</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_20690, AEX15_20730</td></tr><tr><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">15</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">oxidoreductase, inner membrane metabolite transport protein, sorbitol dehydrogenase, fructose-bisphosphate aldolase, kinase.</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">9227</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A0714, SeKA_A0725 <xref ref-type="table-fn" rid="t007fn003"><sup>C</sup></xref></td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_08380, AEX15_08375 <xref ref-type="table-fn" rid="t007fn003"><sup>C</sup></xref></td></tr><tr><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">16</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">putative membrane transport protein, mandelate racemase/muconate lactonizing enzyme, putative transcriptional regulator</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">3481</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A3461, SeKA_A3465</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_14060, AEX15_14050</td></tr><tr><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">17</td><td align="left" style="background-color:#FFFFFF" rowspan="1" colspan="1">peroxisomal (S)-2-hydroxy-acid oxidase, putative glycolate oxidase</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">1202</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">SeKA_A1070, SeKA_A1073</td><td align="center" style="background-color:#FFFFFF" rowspan="1" colspan="1">AEX15_10030, AEX15_10060</td></tr></tbody></table></alternatives><table-wrap-foot><fn id="t007fn001"><p><sup>A</sup> = based on start/stop positions of proten encoding genes within region</p></fn><fn id="t007fn002"><p><sup>B</sup> = flanking loci</p></fn><fn id="t007fn003"><p><sup>C</sup> = encodes a string of homologous sequences but with unique flanking regions/may be unidirectional hits.</p></fn><fn id="t007fn004"><p><sup>D</sup> = putative genomic island by as determined by IslandViewer3 [<xref rid="pone.0161225.ref041" ref-type="bibr">41</xref>].</p></fn><fn id="t007fn005"><p><sup>E</sup> = part of this region predicted to be a genomic island by IslandViewer3 [<xref rid="pone.0161225.ref041" ref-type="bibr">41</xref>].</p></fn><fn id="t007fn006"><p><sup>F</sup> = not detected in ARS-CC7487</p></fn><fn id="t007fn007"><p><sup>G</sup> = truncated in CVM N45934</p></fn></table-wrap-foot></table-wrap><p>Seventeen contiguous regions of ST152 genomes were determined to be absent in ST198 genomes by a similar reciprocal BLASTP analysis. Eight of these regions were identified as putative genomic islands. Several of these regions were annotated as arrays of hypothetical proteins with no known function. Other islands encoded ORFs annotated as being involved in metabolic functions such as region 2 (ORFs involved in glycolysis and gluconeogenesis, inositol catabolism, benzoate degradation) (SeKA_A0372, SeKA_A0399), region 9 (ORFs involved in glycolysis and gluconeogenesis, mannitol utilization) (SeKA_A2624, SeKA_A2631), and region 15 (sorbitol dehydrogenase, fructose-bisphosphate aldolase) (SeKA_A0714, SeKA_A0725). The presence of these operons in strains frequently isolated from dairy cows and poultry suggests their roles in these environments should be further investigated.</p><p>In both STs ten homologous insertion loci flanking different protein encoding regions were identified, indicating that they may be hotspots for integration of laterally transferred DNA in <italic>S</italic>. <italic>enterica</italic>. Five insertion loci encoded tRNA sequences and one encoded a tmRNA sequence, which are known to be regions of genomic island insertion [<xref rid="pone.0161225.ref069" ref-type="bibr">69</xref>]. For the most part the ST-specific islands were conserved among all members of each ST indicating that they may play a significant role in the ecology of these strains and/or they are anchored in the genomes of either host ST.</p></sec></sec><sec sec-type="conclusions" id="sec010"><title>Conclusions</title><p>Based on the analysis conducted herein, there is a phylogenetic difference between poultry-associated and bovine-associated North American <italic>S</italic>. Kentucky ST152 isolates which is discernible due to four core-genome SNPs. However, several clusters of bovine-associated <italic>S</italic>. Kentucky ST152 isolates are more closely related to some poultry-associated isolates than they are to other bovine-associated isolates. This divergence is also associated, <italic>in silico</italic>, with the presence of several plasmids, one of which (ColV) has been demonstrated to enhance the colonization capacity of these strains in the chicken gastrointestinal tract, the other being a high frequency IncX1 plasmid with no known biological role. The influence of the core-genome SNPs and this IncX1 plasmid on the ecology of <italic>S</italic>. Kentucky ST152 in the bovine and poultry hosts, or survival in food sources or the environment should be further evaluated <italic>in vivo</italic>.</p><p>A significant difference in gene content and core-genome nucleotide sequence divergence between <italic>S</italic>. Kentucky ST152 and ST198 isolates was also observed. Based on the methods used in this study, both sequence types are phylogenetically more closely related to other serovars than they are to each other and their shared nomenclature stems from the high level of similarity between their antigenic coding regions which may have transferred laterally between sequence types. Several genomic elements in ST198, such as a sialic acid transport region, inositol catabolism and a homolog of the Typhi colonization factor (tcf), and differences in amino acid sequence of virulence-associated proteins may result in different interactions in the human, bovine, and poultry gastrointestinal tracts, an area that requires further research.</p><p>Although the predominant <italic>S</italic>. Kentucky strains recovered from both dairy cows and poultry in the United States do not appear to cause considerable disease in the animal hosts, the apparent high prevalence in these food animals represents a food safety risk to consumers of beef, dairy, and poultry products. Although infrequent, human clinical salmonellosis caused by <italic>S</italic>. Kentucky has been reported in the United States, but there is not much available data on the sequence types of human-clinical <italic>S</italic>. Kentucky in this country. The recent establishment of CIP<sup>R</sup><italic>S</italic>. Kentucky ST198 in poultry in France and Poland and other regions along with the detection of this ST in dairy cows, raw milk, ground beef, and ground turkey suggests the presence of this potential emerging pathogen should be monitored in food-producing animals.</p></sec><sec sec-type="supplementary-material" id="sec011"><title>Supporting Information</title><supplementary-material content-type="local-data" id="pone.0161225.s001"><label>S1 Table</label><caption><title>Genbank/SRA accession numbers for genomes sequenced for this study.</title><p>(XLSX)</p></caption><media xlink:href="pone.0161225.s001.xlsx"><caption><p>Click here for additional data file.</p></caption></media></supplementary-material><supplementary-material content-type="local-data" id="pone.0161225.s002"><label>S2 Table</label><caption><title>Plasmids identified in each genome analyzed in this study.</title><p>(XLSX)</p></caption><media xlink:href="pone.0161225.s002.xlsx"><caption><p>Click here for additional data file.</p></caption></media></supplementary-material><supplementary-material content-type="local-data" id="pone.0161225.s003"><label>S3 Table</label><caption><title>SNP differences between all <italic>S</italic>. Kentucky ST152 genomes and all <italic>S</italic>. Kentucky ST198 genomes.</title><p>(XLSX)</p></caption><media xlink:href="pone.0161225.s003.xlsx"><caption><p>Click here for additional data file.</p></caption></media></supplementary-material><supplementary-material content-type="local-data" id="pone.0161225.s004"><label>S4 Table</label><caption><title>Annotation of SNPs in protein coding genes identified in an analysis of all <italic>S</italic>. Kentucky ST152 genomes versus all <italic>S</italic>. Kentucky ST198 genomes.</title><p>(XLSX)</p></caption><media xlink:href="pone.0161225.s004.xlsx"><caption><p>Click here for additional data file.</p></caption></media></supplementary-material></sec></body><back><ack><p>We gratefully acknowledge Jakeitha Sonnier for laboratory assistance. This project was supported by internal USDA/ARS research funding. 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